The animal experiments,clinical researches and epidemiological investigations indicated that genistein has the effect for prevention and treatment of osteoporosis,cardiovascular disease and cancer.Genistein can inhibit cellular and humoral immunity and can regulate cell apoptosis.The chemical structure,biological effects and the mechanism of genistein attracted much attention.The current research advance on genistein was summarized and the prospect on the theoretical significance,clinical importance was presented in this paper.

Hypothyroidism is one of the most common endocrine disorders in childbearing or pregnant women.Gestational hypothyroidism can increase the incidence rate of the variety of pregnancy complications and adverse pregnancy outcome.It also can make adverse effects on the fetus and neonate,such as death,placental abruption,fetal malformation,intrauterine growth retardation and preterm,low birth weight,neonatal respiratory distress syndrome,it can also cause abnormal thyroid function of neonate,severely can cause nerve,mental,physical and mental developmental abnormalities and even death.Therefore,it is recommended that childbearing women who are at high risk for pregnancy should strengthen to monitor pregnancy thyroid function and treat as soon as possible in order to reduce the adverse effects on offspring.

Diagnosis, Differential ; Female ; Humans ; Immunohistochemistry ; In Situ Hybridization, Fluorescence ; Leukemia, Myeloid, Chronic-Phase ; genetics ; Lymphoma ; classification ; diagnosis ; pathology ; Male ; Neoplasms ; genetics ; metabolism ; pathology ; Oncogene Proteins ; metabolism ; Prognosis ; Prostatic Neoplasms ; genetics ; Reverse Transcriptase Polymerase Chain Reaction ; Translocation, Genetic ; Tumor Suppressor Proteins ; metabolism

Vertical root fracture (VRF) is different from odontoclasis caused by trauma or injury. VRF is defined as a complete or incomplete fracture of roots caused by long-term and persistent stimulus, such as excessive forces from mastication or occlusion, improper tooth anatomy, and tooth treatment accident. Early diagnosis of VRF is essential to prevent the absorption of alveolar bone, thereby improving prognosis. Radiographic examination is the most common and effective evaluation method for VRF. Cone beam computed tomography can provide three-dimensional information for fracture details, which are more precise than traditional periapical films. In this paper, we present the radiographic features, differential diagnosis, and new treatment techniques for VRF.
Cone-Beam Computed Tomography ; Humans ; Tooth Fractures ; Tooth Root

Objective:To investigate whether the fish oil improves intestinal motility function during intra-abdominal sepsis and hypertension.Methods: 25 rats were divided into three groups.The rats were supplemented by gavage with: ①normal saline as control group(n=9);②fish oil(0.3%,n=8)and ③fish oil(0.6%,n=8)for 4 weeks before operation.The rats were killed on the 24th hour after operation.In vitro ileual smooth muscle contractions were recorded.Ileum sections were assessed pathologically.Immunopositivity of c-kit as markers of ICC-MY by confocal microscopy were evaluated.Results: In the bowel segment with NS,muscles showed a significant reduction in the frequency of contraction compared to that with FO.This change was associated with the changes of ICC-MY network.Conclusion:The intestinal motility may be protected by an enteral fish oil nutrition through attenuating injury of ICC-MY network.

Objective:To investigate whether an enteral fish oil immunonutrition during intra-abdominal sepsis and hypertension dose improve pulmonary function.Methods: We divided 25 rats into three groups.The rats were supplemented by gavage with:(Ⅰ)normal saline as control group(9);(Ⅱ)fish oil(0.3%,8) and(III)fish oil(0.6%,8).The cytokine levels were analyzed.The lungs were harvested,stained with hematoxylin-eosin,and analyzed. Results:Intra-abdominal sepsis and hypertension induced thickening of alveolar walls and inflammatory reaction.Fish oil decreased expression of pro-inflammatory cytokines(TNF-? and IL-1?),inhibitted infiltration of MPO positive cells,and protected pulmonary structure.Conclusion: The lung may be protected by an enteral fish oil nutrition through attenuating inflammatory reaction.

Objective To detect the phenotype and genotype of 11 vancomycin-resistant enterococci(VRE).Methods Disk diffusion test,microdilution broth method,VITEK-2 and Etest were used to determin the susceptibility of 11 VRE to vancomycin and teicoplanin. MIC to 10 antimicrobial agents were determined with microdiluton broth method. vanA, vanB ,vanC_1, vanC_2 were detected with multiplex PCR and were further confirmed by DNA sequencing. TEM, tetM,ermB,aac(6′)/aph(2′′),ant(6)- I,aph(3′)-Ⅲ were detected with PCR.Results Susceptibilities of 11 VRE to vancomycin and teicoplanin were different in four vitro susceptibility testing methods. VRE showed resistance to erythromycin(9/11),ciprfloxacin(7/11),levofloxacin(7/11),rifampin(8/11) and chloramphenicol(7/11). HLGR and HLSR is 10/11 and 9/11 respectively. 1 VanA,4 VanB,5 VanC_1 and 1 VanC_2 were detected and their phenotypes confirmed to the genotypes. High rate of TEM(8/11), tetM(6/11), ermB(7/11), aac(6′)/aph(2′′)(7/11), ant(6)- I(5/11) and aph(3′)-III(9/11) were detected in 11 VRE.Conclusions VRE screening test and the determination of MIC are reliable in finding VRE. PCR is precise and specific in the testing and confirmation of VRE. The high rates of drug resistance genes showed the complicated mechanisms of drug resistance in VRE.

Objective To explore the characteristics of TCM syndromes of acute coronary syndrome(ACS). Methods Clinical data of 127 ACS patients were collected and then a database was established.After that,the distribution of syndrome patterns was analyzed.Results The incidences of the following excess syndrome patterns were in a decreasing sequence:stasis syndrome,phlegm syndrome,cold syndrome and heat syndromes.The incidence of stasis syndrome was 90.5%,which was much higher than other excess syndromes.The incidences of the following deficiency syndrome patterns were in a decreasing sequence:heart-Qi deficiency syndrome,heart-Qi exhaustion syndrome,heart-yin deficiency syndrome,heart-Qi depletion syndrome,heart-kidney deficiency syndrome and heart-blood deficiency syndrome.The incidence of heart-Qi deficiency syndrome was 75.6%,which was much higher than other deficiency syndromes.The incidences of the stasis syndrome and cold syndrome in acute myocardial infarction(AMI)were higher than those in unstable angina(UA),and the incidences of heart-Qi deficiency syndrome and heart-Qi exhaustion syndrome in AMI were higher than those in UA(P

Objective To study the clinical analysis in the neonatal hypoxic-ischemic encephalopathy (HIE)for evealuation of the severity of brain injury and prognosis. Methods The manifestation,laboratory findings,outcomes of 117 HIE were analyzed. Results Manifestations of HIE in 117 infants included 3 groups(minor level,moderate level,severe level). There was no significant difference in the weight and gestation-age among 3 groups.Scores of Apgar lowing,which Was marked in the severe level(P＜0.01). There was significant difference in the level of pH and PaCO2(P＜0.01). There was significant difference in the level of blood GLU and BE reduced aboviusly in the severe level,and in the level of BUN,CK-MB,CK increased aboviusly in the moderate and severe level,among all(P＜0.01). 69 patients were taked CT on the brain,the abnormal rate of which was 82.6%. The abnormal rate of CT on the brain increased 100% changing with the severity of brain injury on HIE especially in the moderate and severe level. Conclusion The prognosis of the minor level of brain injury on HIE is the best. The prognosis of the moderate-level group who was treated reasonably and enough,is better mostly.

Xenogeneic/Allogeneic bone materials are among the hot research interests of biological scaffolds.These materials are ideal materials for repairing bone defects because they have the structure and morphology of the natural bones,are able to be degraded and absorbed in the body,and are less immunogenic through appropriato treatment.Through further modification with biological molecules such as growth factors,they could provide a better microenvironment for the bone cells to grow into and fully differentiate.This article summarizes the factors affecting the immunogenicity of xenogeneic/allogeneic bone and the methods of removing immunogenicity.Advances in sintered bone materials,defatted bone materials,deproteinated bone materials,decalcifying bone materials,and composite materials are reviewed in greater details.