Nitric oxide (NO) is a vascular endothelium-derived relaxing factor.NO inhalation therapy is widely used in the field of neonatology.When NO acts as selective pulmonary vasodilation,it can reduce pulmonary arterial pressure and improve oxygenation.When NO serves as a major regulator of cerebral vasomotor factor,it may have physiological and pathological impact in cerebral ischemia-reperfusion injury.So NO has a wide range of applications in persistent pulmonary hypertension of the newborn,bronchopulmonary dysplasia,hypoxia ischemic encephalopathy.With the recently in-depth study of NO,its biological role has more and more clinical attention in the field of neonatology and has broad valid prospects.However,NO has some side effects which still have some controversial aspects of the application in premature,especially in very low birth weight children.The article focused on the mechanism and clinical applications of NO in the neonatal disease.

Objective To investigate the clinical effect of integrated care model in improving the quality of life and enthusiasm of treatment in patients with cardiovascular diseases. Methods 60 patients in department of cardiology adopting integrated care from September 2008 to April 2011 were chosen as the observation group.60 patients with ordinary care were set as the control group.The quality of life and the situation for enthusiasm of treatment were taken for statistical analysis. Results Patients with good quality of life in the observation group was 39 cases (65.00％),more than 21 cases (35.00％) in the control group.Patients obtaining effective treatment result in the observation group was 48 cases (80.00％) and 15 cases (25.00％) in the control group,there was significant difference.It was statistical significant.Adherence to medication,reasonable diet,adequate exercise and proper understanding of disease status in the observation group were significantly better than the control group. Conclusions Application of integrated care models in nursing of disease and life of patients with cardiovascular diseases can improve the quality of life and enthusiasm of treatment of patients,which is better than general model of care.

There are multiple genes involved in the progress of evolution in breast cancer.Recent study showed that Twist gene may play key role in promoting tumor growth,invasion and metastasis through the epithelial-mesenchymal transition (EMT).Other studies also demonstrated that Twist gene expression maybe associated with patient outcomes and guiding clinical tailored therapies.

Objective To understand cerebral edema and development procedure of different time windows.Methods To analyze their prognosis through image document and operation findings of 52 cerebral trauma patients which were confirmed cerebral edema by CT and operation.Results he light cerebral oedema patients have a better prognosis,10 patients died and 1 patient became a plant man in 11 malignant brain dilatation patients.Conclusion The infernal circle of high brain pressure-cerebral ischemia,cerebral anoxia-cerebral edema result in cerebral edema-brain dilatation-malignant brain dilatation after cerebral trauma which leads to unremitting aggravated pathogenefic condition.So effective means should be used to interrupt the unremitting heightening ICP.

To explore the stability of impact assay for determination of microorganism in indoor air, the comparisons of continued sampling times of 2-minute and 4-minute for monitoring on microorganism of indoor air were carried out with JWL- Ⅱ B multifunctiom air microorganison monitor in 11 hotels, public places of entertainment, barbar shops and beauty shops. The results showed that, under a same environmental condition, there was no significant differences between the data obtained by 2-minute monitoring and 4-minute monitoring (t=1.854, P＞0.05).It indicated that impact assay presented very good stability in detecting the microorganism of indoor air within certaindetecting duration.

Objective To analyze the mechanism of hemiparalysis children,cerebral vessels' physiological feature of children, pathological change and therapeutic efficacy after light craniocerebral trauma. Methods To analyze clinical manifestation,imageology data,therapy and therapeutic efficacy of 32 hemiparalysis children patients after light craniocerebral trauma. Results Mon-basal ganglia cerebrel infarction was discovered in all children,and cure rate of broadening blood vessel therapy was 84% in a week after trauma. Conclusion Blood vessel spasm and thrombosis happened after light craniocerebral trauma to artery of cerebral hemorrhage that erupt perpendicularly from arteria cerebri media and had incomplete tunica intima development. Nonage broadening blood vessel therapy could gain well curative effect.

Objective To study the correlation of blood uric acid,lipoprotein associated phospholipase A2(Lp-PLA2) and arterial stiffness in young patients with coronary heart disease.Methods 56 young patients with coronary heart disease were selected as the observation group,50 young healthy subjects were selected as control group.The serum uric acid,Lp-PLA2 and arterial stiffness in the two groups were determined.Blood uric acid,Lp-PLA2 and arterial stiffness in patients with coronary heart disease were analyzed.Results There were no significant differences in age,sex,BMI,systolic blood pressure,diastolic blood pressure and triglyceride between the two groups (all P>0.05).The total cholesterol,low density lipoprotein,uric acid and Lp-PLA2 in the observation group were significantly higher than those in the control group (t=3.927 4,4.162 4,5.772 6,9.770 1,P<0.05,P<0.01).High density lipoprotein in the observation group was significantly lower than that in the control group (t=2.6043,P<0.05).Multivariate Logistic regression analysis showed that low density lipoprotein cholesterol,uric acid,Lp-PLA2 were independent risk factors for coronary heart disease (low density lipoprotein uric acid OR=1.053,95% CI:1.031-1.106;uric acid OR=1.082,95% CI:1.043-1.115;Lp-PLA2 OR=1.007,95% CI:1.002-1.011).The arterial stiffness of the observation group was (8.86±1.63)v·cm-1·s-1,and had positive correlation with serum uric acid (r=0.221,P<0.05) and Lp-PLA2 (r=0.361,P<0.05).Conclusion Blood uric acid,Lp-PLA2 can reflect the early arterial stiffness in young patients with coronary heart disease.

Looking back to the history of the 20th century contributes to the rise of bioethics and the unification of international law on the present age.In the times of rapid scientific and economic development,it is either a core issue of bioethics or a starting point of international law that how humans should survive and establish a relationship with the nature.And a series of common behavior criteria for human being are being or will being established to seek a co-existent path between peoples as well as between human being and nature.

Objective: To establish a model of rat osteosarcoma in nude mice and to study its biological characteristics.Methods: Intact tumor pieces were inoculated into the right tibia of nude mice.The nude mice were killed at different time and the growth of tumor and metastasis were observed.Result: The tumor growth rate was 100%.From the first week to the second week after implantation it grew into outer tissue from tibia,and widely invaded Soft tissue on the third week.Pulmonary metastasis took place from the fourth week to the fifth week,and the nude mice died of cachexia and respiratory failure resulting from the widely whole pulmonary metastasis on the sixth week or so.Conclusion: The tumor growth rate is high.The model simulates the clinical process of human osteosarcoma,and could be a satisfactory model for studying osteosarcoma.

Objective:To develop an HPLC method for the assay of the content of triptolide in triptolide gel. Methods:The content of triptolide was measured by HPLC on Diamonsil C18 column(250mm?4.6mm i.d,5?m)using methanol-water(46∶54,v/v)as mobile phase,at a flow rate of 1.0ml/min. The UV detection was set at 220nm. Results:The calibration curve was proved to be linear in the range of 10.24~51.20?g/ml with a regression coeficient of 0.999 7. The average recovery of the method was 98.3%;RSD was 0.23%(n=5). Conclusion:This method is found to be rapid and accurate for the measurement of triptolide in triptolide gel.