Objective To determine the levels of radiation-induced expressions of HIF-1α and Survivin in three human lymphoma cell lines with or without HIF-1a specific antagonist or HIF-1a siRNA transfection and to explore the possible mechanism of cell radioresistance.Methods Namalwa,Ramos,Raji cells were irradiated with X-rays with or without the combination of echinomycin (EC),a HIF-1α specific inhibitor,or transfection of HIF-1α siRNA.Then the expression levels of HIF-1α and Survivin proteins and mRNAs were determined by Western blot and real-time PCR,respectively.Results After irradiation,the expression level of HIF-1α protein was up-regulated at 10-20 h followed by up-regulation of survivin protein at 24 h (t =7.53-31.31,P ＜ 0.01 ) in three lymphoma cell lines.Radiation-induced up-expression of survivin protein was significantly reduced by either HIF-1α inhibition or EC treatment (t =7.21-32.81,P ＜0.01 ).However,radiation failed to induce up-erpression of survivin at both mRNA and protein levels after transfection with HIF-1α siRNA.Conclusion HIF-1α-Survivin pathway is activated in human lymphoma cells after radiation,which might be involved in the cell radioresistance.

Objective To investigate the effect of recombinant human erythropoietin (rhEPO)injection on the nutritional quality and cardiac function in the patients with maintenance hemodialysis.Methods Sixty-two patients with chronic kidney failure were divided into two groups by random digits table:treatment group(46 patients, rhEPO injection therapy was given) and control group( 16 patients, without rhEPO injection therapy). The serum biochemical indexes and color sonography was respectively performed before and after the treatment. Results In treatment group, the cardiac function and the serum biochemical indexes improved obviously after 6 months' treatment (P ＜ 0.05 ). But there was no change in control group.Conclusion The treatment with rhEPO injection can effectively improve the nutritional quality and cardiac function in the patients with maintenance hemodialysis, and improve life quality.

Food allergy is a common illness in children,which is divided into IgE-mediated,non-IgE mediated and mixed-mediated food allergy.Gastrointestinal tract is the mainly affected organ,mostly mediated by non-IgE mechanism.Gastrointestinal food allergy are lack of unified diagnostic criteria currently,whose mechanism is also elusive.Moreover,the double-blind placebo-controlled food challenge is the gold standard for the diagnosis of food allergy.This review focuses on the procedure for the oral food challenge and its application in the diagnosis of gastrointestinal food allergy.

Most epidemiological investigations and basic studies have demonstrated that estrogen has neuroprotective effects,and the application of estrogen in cerebrovascular disease has a positive attitude.However,large clinical trials of the application of estrogen have indicated that harms outweigh benefits,and even have the risks of stroke.This article reviews the related studies about the neuroprotective effects of estrogen from the positive and negative aspects.

The post-stroke depression refers to a affective disorders within 2 to 3 years after stroke in patients with stroke.Its main features are depressed mood,decreased interest,irritability,pessimism,and slow thinking,etc.Usually the symptoms will last for more than 2 weeks.It is one of the most common complications of stroke and may seriously affect the rehabilitation and quality of life of patients.Some patients even have suicidal tendency.Early intervention is very important for late neurological recovery in patients with stroke.It can not only improve cognitive function,but also reduce the morbidity and mortality.This article reviews the treatment of post-stroke depression.

With the development of genomics , bioinformation , engineering , computer science and other fields, it has witnessed explosive growth of molecular diagnostic technologies.More and more technologies are used in desease diagnosis , therapy and prevention , which have presented a huge opportunity and challenge for clinical laboratory.Each technology has corresponding field of application , so it is a crucial problem for clinical laboratory to select appropriate molecular diagnostic technology for personalized medicine.

Recent studies have found that inflammatory response is positively associated with the progression of liver cirrhosis,acute-on-chronic liver failure,and primary liver cancer and can affect their prognosis,and as a marker for inflammatory response,neutrophil/lymphocyte ratio (NLR) is easy to calculate,has good repeatability,and holds promise for prognostic evaluation.This article introduces the research advances in the association of NLR with the prognosis of liver cirrhosis,acute-on-chronic liver failure,and primary liver cancer and points out that NLR plays an important role in evaluating the prognosis of liver cirrhosis,acute-on-chronic liver failure,and primary liver cancer.However,there are still controversies over the selection of optimal cut-off values for different diseases,and further studies are needed.

Objective To study the relationship between serum lactadherin (MFG-E8),sialic acid (SA) level and coronary heart disease (CHD) occurrence and development.Methods 166 patients in our hospital were selected from June 2014 to November 2015,and then according to the result of coronary angiography divide the subjects into control group (n=56,the result of coronary angiography completely normal) and CHD group,including stable angina 48 cases (StA subgroup),unstable angina 30 cases (UA subgroup) and acute myocardial infarction 32 cases (AMI subgroup).The serum level of MFG-E8 was detected by ELISA method,the serum level of SA,hs-CRP and lipid index were detected by biochemical analysis.Gensini score showed the severity of coronary stenosis.The correlation analysis between detecting parameters was carried out by Spearman method.Results Compared with the control group,the patients with CHD have a higher level of serum SA and a lower level of MFG-E8 (both P<0.05).The AMI subgroup has a lower level of serum MFG-E8 than StA and UA subgroups,and the AMI subgroup has a higher level of serum SA than StA and UA subgroups (all P<0.05).The correlation analysis showed that the serum SA has a positively correlation with the level of TG,hs-CRP,and Gensini score,and has a negatively correlated with the level of MFG-E8 (all P<0.05).The MFG-E8 has a negatively correlated with the level of TG,hs-CRP,and Gensini score (all P<0.05).Conclusion MFG-E8 and SA play an important role in the development of CHD,and combined detection of serum MFG-E8 and SA may be helpful in the clinical diagnosis and risk assessment of patients with CHD.

In order to detect the expression of RhoA in placenta from normal pregnancy and preeclampsia and evaluate the role of RhoA in preeclampsia, the expression of RhoA in placenta collected from 40 preeclampsia patients and 20 normotensive controls was determined by immunohistochemistry and RT-PCR. RhoA was found in syncytiotrophoblasts and cytotrophoblasts. The mean density of RhoA protein in placental tissues of mild and severe preeclampsia groups was significantly higher than that in normal pregnancy. The expression level of RhoA mRNA in mild and severe preeclampsia groups was significantly higher than that of normal pregnancy. Increased expression of RhoA in placental tissues might play an important role in the pathogenesis of preeclampsia.