Objective:To analyze the clinical data of primary pulmonary hypertension in children and improve its clinical diagnosis and treatment.Methods:The clinical data of 6 patients with primary pulmonary hypertension were collected and analyzed.Clinical diagnosis was made by ways of electrocardiography,ultrasound,and X-ray.All of them were received catheterization examination and angiography.Results:Heart failures were found in all patients with PPH.Signs of pulmonary hypertension were found by accessory examination.No heart malformation was found by catheterization examination.Conclusion:There are all kinds of clinical manifestations of PPH.The definite diagnosis of PPH will rely on catheterization examination.

Objective:Analysis of complex congenital heart disease of children and the incidence of coronary abnormalities to explore the21(4):453-457. diagnostic value of coronary angiography.Methods:Angiocardio graphy cardiac catheterization of 229 children in Heart Center of Children's Hospital Chongqing Medical University from January 2006 to June 2009,the results were retrospectively analyzed,including the types and proportion of congenital heart diseases,incidences of coronary artery abnormalities,incidences of abnormal coronary artery crossing right ventricular outflow tract and prognosis.Results:Altogether,27(11.8%)cases of children with Congenital Coronary Artery Anomalies were detected.Highest incidenc(e6.55%)is L-coronary artery,which occupy 55.56% of Coronary Artery Anomalies.16(6.99%) cases of abnormal coronary artery crossing the right ventricular outflow tract,which including 7 cases of TOF,3 cases of DORV,3 cases of PA/VSD,1 case of TGA,1 case of CAVC and 1 case of SV/TGA.1 patient died of angiocardiography among the 27 cases of complicating coronary artery malformation,accounting for 3.7%in the total number of the angiography of complex congenital heart disease complicating coronary artery malformation,accounting for 0.44%in the total number of complex congenital heart disease.3 cases died after surgery, accounting for 16.7% of surgery.Conclusion:The incidence of coronary artery abnormalities in complex congenital heart disease in children is higher than the normal population;coronary angiography is an important means of final diagnosis coronary artery congenital variation.

China ; epidemiology ; Humans ; Metabolic Syndrome ; epidemiology ; prevention & control

Micrometastasis are frequently accompanied by the majority of cases of invasive malignant tumor, being a compacted relationship with the prognosis of patients. We should pay more attentions to the occurrence of micrometastasis in the diagnosis and management of carcinoma of urinary bladder, which is the most commonly diagnosed malignancy in urinary system and a significant cause of morbidity and mortality.This article introduces the diachronic concept of micrometastasis and the major methods for the detections of micrometastasis, moreover,it reviews recent advances about the detection of micrometastasis in bladder cancer, and about the benefits that patients of the disease might have received in the treatment of micrometastasis.

Objective To investigate how genistein to inhibit the invasion of human ovarian serous papillary cystadenocarcinoma cell line SKOV3.Methods Millicell chamber and coculture method were used to establish chemotactic migration model in vitro to observe the effect of genistein on directional chemotactic migration movement of SKOV3 cells.The protein expressions of cell surface adhesion molecule CD44v6,matrix metalloproteinases and tissue inhibitor of metalloproteinases MMP-9/TIMP-1 and MMP-2/TIMP-2 were determined by using immunocytochemical method and their mRNA levels were examined by in situ hybridization.Results Compared with control group,the directional chemotactic migration of SKOV3 were significantly decreased after treated with 20 ?mol/L and 40 ?mol/L genistein,reached to(46.9?5.8)% and(28.3?4.7)% respectively(P

Objective To investigate the inhibitory effect of genistein on the invasion of ovarian carcinoma cell line SKOV 3 in vivo and in vitro. Methods The abilities of the genistein-treated SKOV 3 cells to invade through reconstituted matrigel in transwell chambers were investigated in vitro and the invasion effect in vivo was determined using the xenograft models of SKOV 3 in nude mice. Results The ability of the 20 ?mol/L genistein-treated cells to invade the reconstituted basement membrane was decreased significantly at 72 h. This inhibition was in a dose-dependent manner. Genistein at the dose of 40 ?mol/L had the strongest effect. The results in vivo suggested that the grade of invasion in control SKOV 3 cells was in a time-dependent manner and genistein-treated group could apparently inhibit the progress of invasion, localizing the tumor in invasion grade 0 or grade I, and decreasing the proportion of grades Ⅱ, Ⅲ, Ⅳ. Conclusion The results suggest that genistein has inhibitory effect on the invasion of human ovarian carcinoma cell lines SKOV 3 in vivo and in vitro.

Aldehyde oxidase (AOX), a highly conserved molybdoflavoenzyme in mammal cytoplasm, has broad substrate specificity and ability to catalyze the oxidation of aldehydes and nitrogen, oxygen-containing heterocyclic rings. AOX was found to widely distribute with the individual differences in vivo and plays an important role in phase I metabolism of drugs and xenobiotics. The biological characteristics of AOX and its contributions in drug metabolism are introduced briefly in this review.

Angiogenesis plays an important role in solid tumors and hematologic malignancies. Angiopoietins act as essential regulators in this process,complementary with another mediator-VEGF. In the development of hematologic malignancies, the expression of Angs/Tie-2 system is identified abnormal, especially for Ang-2, whose expression and prognostic impact have gained significant attention recently.

Objective To understand the drug resistance in cancer patients with secondary non-fermenting bacterial lower respir-atory tract infection in order to provide a basis for clinical rational use of antibacterial drugs.Methods The lower respiratory tract specimens were collected from the patients with malignant tumor and identified by the fully automated microbial identification sys-tem,the drug susceptibility test was performed by using K-B method and the drug susceptibility test results were judged according to CLSI 2012 standard.The data were analyzed by the WHONET 5.6 software.Results 172 strains of non-fermenting bacteria were isolated from the lower respiratory tract specimens in the patients with malignant tumors,in which Pseudomonas aeruginosa was maximum,accounted for 45.9%,followed by Acinetobacter baumannii and Stenotrophomonas maltophilia ,accounted for 36. 0% and 10.5 % respectively.The drug susceptibility test showed that five kinds of non-fermenter demonstrated the high resistance or multi-resistance to multiple antibacterial drugs.Conclusion Non-fermenting bacterial multi-drug resistant phenomenon is seri-ous,clinic should pay attention to non-fermenting bacterial infection and drug resistance monitoring,antibacterial drugs should be rationally used according to the drug susceptibility test results in order to reduce the generation of drug-resistant strains.

BACKGROUND:In the whole world, spinal cord injuries caused by trauma lead to more than 180 000 people presenting with permanent impairment annualy. A large number of experiments have confirmed in recent years, under physiological conditions, microRNA has specific expression and plays an important role in the nervous system. OBJECTIVE: To discuss the changes in microRNA expression induced by injuries as wel as the pathophysiological significance in spinal cord injury, and to explore the development potential of microRNA in tissue-engineered and clinical repair of spinal cord injury. METHODS:A computer-based search of PubMed and Chinese Journal Database was performed for related articles published from January 2000 to December 2014 using the keywords of “SCI, microRNA, transcriptional control, clinical research progress” in English and Chinese. Finaly, 38 articles were included for result analysis. RESULTS AND CONCLUSION: Mechanical injury initialy triggers a series of complex secondary damages, including nervous, vascular and immune systems, which can influence the severity of spinal cord injury to a great extent. Secondary damage to the spinal cord is mainly attributed to the activation and deactivation of some specific genes associated with celular and biochemical mechanisms, such as cysteine aspartate specific protease (caspase) gene family, apoptosis related protein Fas and its ligand Fasl system, P53 gene, apoptosis related gene Bcl-2 family. Recent studies have proved that the functional activation of microRNA expression is the key to spinal cord injury. With the development of biological information engineering, studies and controling technologies associated with microRNA expression have been gradualy dominated, some clinical application based on microRNA technology has entered the clinical trial stage. It is believed that with the continuous development of technology and decrease of cost, permanent dysfunction due to spinal cord injury can be regulated and repaired through the microRNA technology at gene level in the future.