In recent years,with the deepening research on autoinflammatory diseases (AID),more and more diseases have been found and included in the AID category.The clinical manifestations of AID are various,but most of them are not specific.Therefore,it is difficult to distinguish AID from infectious diseases,tumors and other autoimmune diseases,which causes misdiagnosis and delayed diagnosis.This article aims at discussing about the clinical diagnosis and treatment procedure of AID.

Abstracting and Indexing as Topic ; Academic Dissertations as Topic

The atrio-ventricular valves of the normal heart in 200 Chinese cadavers of different ages were studied. The right atrioventricular valves were classified into three types and the left were classified into two types, according to the number of the main cusps. It was found that percentage of the second type (53.5%) of the right atrio-ventricular valves was higher than that of the first type (43.5%) and the first type (83.0%) of the left atrio-ventricular valves is in the majority, while the second type (17.0%) represents only a small percentage.An accessory cusp is situated between two main cusps. The accessory cusp was found in the right atrio-ventricular valves of 132 cases (66.0%), the majority of which had only one accessory cusp (95 cases. 72.0%), and among them there were 65 cases (37.4%) in which antero-medial accessory cusps were present. The accessory cusps were found in the left atrio-ventricular valves of 103 cases (51.5%), most of them also had only one cusp (92 cases 89.3%) and among them there were 71 cases (62.3) in which postero-medial accessory cusps were present.If the total number of the main and the accessory cusps was considered, in the right ventriculus, four cusps were found in 40.5% of the cases, five in 29.0%, three in 19.5%, six in 9.0%, two in 1.5%, seven in 0.5%; while in the left ventriculus, three cusps were found in 51.5% of the cases, two in 37.0%, four in 11.0% and five in 0.5%.The size of the valves was measured. In the right ventriculus the postero-medial valve is the broadest (40.1 mm), the postero-lateral valve is the highest (24.5 mm). In the left ventriculus the postero-lateral valve is the broadest (36.9 mm), the antero-medial valve is the hightest (20.8 mm). The associated valves are usually larger than the other common main valves, the diameters of which are larger than that of the accessory valves. The perimeter of left artrio-ventricular ring is 79.69 mm and the right is 97.79 mm.

Spontaneous abortion is one of the most common gestational conditions, with the morbidity of 1% to 5% in married women. It is a serious problem troubling women at the reproductive age. There are many factors causing spontaneous abortion, among them, spontaneous abortion resulting from endocrine abnormalities can be detected and treated. Prolactin is a major endocrine hormone participating in reproductive regulation and playing an important role in early pregnancy implantation and pregnancy maintenance. When there are unusual levels it is closely related to spontaneous abortion, leading to a necessity to further study the relationship between them. International and domestic research developments in recent years about the correlation between prolactin and spontaneous abortion are discussed in this paper.

According to existing problems in polysaccharide determination methods in new Chinese drug applications, the method suitability, chemical reference selection, components interference and method research were introduced. The author suggests that suitable determination method should be selected according to the structure and property of the polysaccharide, and validated. Some influent factors should be examined to assure the accuracy of the method, such as the stability and using amount of the visualizing reagent, visualizing time, maximum detection wavelength etc. Monosaccharide and other water soluble components should be removed from polysaccharide sample, and suitable reference substance and detection wavelength should be selected. It should pay attention to mutual interference of neutral and acidic saccharide, and use inhibitor to eliminate the interference. Because the slopes of the standard curves are different for different monosaccharide, it is proposed that the types and ratios of the monosaccharide in heteroglycan should be understood, and mixed reference substance solution in the ratio is prepared for determination.
Drugs, Chinese Herbal ; chemistry ; Monosaccharides ; analysis ; isolation & purification ; Polysaccharides ; analysis ; isolation & purification ; Research ; trends ; Technology, Pharmaceutical ; methods ; trends

BACKGROUND: Migraine is usually located in the course of Shaoyang channel, and the curative effect of treatment with methods of treating Shaoyang disease by dispersing and dispelling wind (DW) as well as relieving pain (RP) on migraine is prominent, but how about the effects of these two ways on thrombogenesis in vitro and the function of platelet aggregation (PA)?OBJECTIVE: To probe into the effect of treatment of Shaoyang disease and methods of DW and RP on thrombogenesis in vitro and function of PA throughthe experiments of thrombogenesis in vitro and function of PA in rats, and investigate the compatible significance of DS and DW as well as RP through formula compatible experiment.DESIGN: Completely randomized grouping design and controlled trial.SETTING: Department of Prescription, College of Basic Medical Sciences,Heilongjiang University of Chinese Medicine.MATERIALS: The experiment was conducted in the Laboratory of Department of Prescription, Heilongjiang University of Traditional Chinese Medicine from March to August 2000. Sixty healthy adult Wistar rats were randomly divided into 6 groups: Normal control group, Shaoyang treatment group (group Ⅰ ), DW and RP group (group Ⅱ ), high-dose and whole-prescription group (group Ⅲ), low-dose and whole-prescription group (group Ⅳ), and positive control group with 10 rats in each group.METHODS:① Positive control group:Gastric perfusion of 0.034 g/mL compound divaside slice suspension (made by Harbin Second Biochemical Pharmacy co., Ltd) was given to rats (0.39/kg); Group Ⅲ and Ⅳ (ingredients: Chaihu 20 g, huangqin 10 g, banxia 15 g, gancao 10 g, chuanqiong 20 g, tianma 15 g, xixin 5 g, quanxie 5 g, wugong 5 g): Gastric perfusion of 1.5, 0.75 g/mL of complete prescription was given to rats (17.40, 8.70 g/kg);Group Ⅰ (ingredients: Chaihu 20 g, huangqin 10 g, banxia 15 g, gancao 10 g) and group Ⅱ (ingredients: Chuanqiong 20 g, tianma 15 g, xixin 5 g, quanxie 5 g, wugong 5 g): Gastric perfusion of 0.75 g/mL agents of treatment with DS and DW wind antidyne was given to rats as 8.70 g/kg; Normal control group: Gastric perfusion of normal saline of the same volume wasgiven to rats.Intervention on rats of all groups lasted 12 days.② Experiment of thrombogenesis in vitro: The cephalic artery of one side was seperated 2 hours after the last time of administration. The distal part was deligated, the blood current in proximal part was blocked with bulldog clamp and the arterial cannula was inserted into the cephalic artery. Loosened the bulldog clamp, blood of 1.8 mL was collected and put into the rotary ring of thrombogensis meter to rotate for 15 minutes at 17 r/min,and then poured the thrombus, measured the length and humid weight of thromb. After that,dried the humid thromb and measured the dry weight.③ Determination of PA:The packing fraction (PF) in the 1st and 5th minutes as well as the maximal packing fraction (MPF) and assembling inhibition ratio of platelet-rich plasma within 5 minutes were recorded with test.MAIN OUTCOME MEASURES: Effect of treating Shaoyang disease nephelometry.④ Differences of measurement data were compared with tand methods of DW as well as RP on thrombogenesis in vitro and the function of PA.RESULTS: A total of 60 rats were involved in the analysis of results. ①The length, humid weight and dry weight of thrombogenesis in vitro in group Ⅲ and Ⅳ, positive control group and group Ⅱ were obviously lower than those in group Ⅰ (P ＜ 0.05). ② The PF in 1st, 5th minute and the maximal aggregathe normal control group (P ＜ 0.05-0.01); The humid weight and dry weight of thromb in group Ⅳ were significantly lower than those in tion (MA) of blood platelet in rats of each group were significantly lower than those in the normal control group (P ＜ 0.05-0.01), and the curative effect on rats in the positive control group, group Ⅲ and Ⅳ as well as group Ⅱ were better than that in group Ⅰ.CONCLUSION: There are no significant effects of dispersing thrombogenesis in vitro and inhibiting PA by only treating Shaoyang disease. The effect of combined prescription of DW and RP as well as treatment of Shaoyang disease are obviously enhanced.

The current enrollment system, curriculum, teacher quality, the quality of doctoral thesis research and scientific research and innovation ability are the main factors influencing the quality of doctoral cultivation of traditional Chinese medicine. By analyzing these influence factors, the article try to explore traditional Chinese culture quality assurance factors of doctoral students, that is, to control the quality of students, to clear training objectives, to strengthen the responsibility of instructors, to supervise the quality and training of scientific research and innovation ability of students and so on.

Gastric cancer is a malignant tumor with the second highest incidence and mortality in the world, and China is one of the countries with the highest incidence of gastric cancer in the world. Research on the regulation of cell cycle protein can provide effective evidence for the mechanism of tumor and gene therapy.G1/S phase is the key point for cell cycle regulation,Cyclin D1 and CDK4 gene and P16 play important roles in this phase and have great correlation with gastric cancer,but the exact relationship between these genes and gastric cancer is still unclear. In order to better understand the expression of Cyclin D1 and CDK4 gene and P16 in gastric cancer, the related researches in recent years were summarized in this paper.

OBJECTIVE:To provide reference for the rational use of TCM injections. METHODS:The related data of using TCM injections was drawn from May 2009 to May 2014. And the data of 5 TCM injections,including Shengmai and Shenmai in-jection,were statistically analyzed,the indications of 8 TCM injections,including Shengmai and Shenmai injection,were also sta-tistically analyzed. RESULTS:In solvents evaluation,the 5 TCM injections were used 501 times,in which 391 times(78.04%)of the solvents used were rational,381 times(76.05%)of the solvent doses used were rational;in clinical indications evaluation,the 8 TCM injections were used for 2 579 patients,in which 1 120 cases were in accordance with the instructions while the other 1 459 cases were not,the qualification ratio was 43.43%. CONCLUSIONS:There were still some problems in using TCM injection in our hospital in the aspects of solvent as well as indications.

Objective To further study the pathogenesis for congenital heart disease (CHD) and connective tissue growth factor(CTGF) for the early diagnosis of CHD and pulmonary hypertension (PH).Methods Sixty-six cases of children with CHD were selected from the thoracic surgery who were performed in the Children's Hospital of Shanxi Province during the period between Sep.2012 and Jan.2013.Based on the cardiac color Doppler estimation of the pulmonary artery systolic pressure,they were classified into several groups:unincorporated PH group with 16 cases,mild PH group with 18 cases,moderate PH group with 17 cases,and severe PH group with 15 cases.Based on types,the 66 cases could be classified as ventricular septal defect (VSD) group with 23 cases,atrial septal defect (ASD) group with 17 cases,patent ductus arteriosus(PDA) group with 14 cases,and composite(including 2 or more of the congenital cardiovascular anomalies) group with 12 cases.Twenty healthy children who had medical examination over the same period in the Children's Hospital of Shanxi Province were randomly chosen as the healthy control group.The serum concentration of CTGF in the research objects were determined with the aid of double antibody sandwich enzyme-linked immunosorbent assay method.According to the different pulmonary artery systolic pressure and different types of CHD,the serum concentration of CTGF were compared among groups.The correlation of between the CTGF concentration in the serum and pulmonary artery systolic pressure were also analyzed among the groups of children with CHD.Results Quantitative measurement revealed that the serum CTGF concentration measured in the groups of children with CHD were significantly higher than that in the healthy control group(P ＜ 0.05) ; the serum CTGF concentration of the children with CHD and PH was higher than that in the group of children with CHD alone(P ＜ 0.05).With the increase in pulmonary artery pressure,CHD patients serum levels of CTGF also increased(P ＜ 0.05).By the correlation analysis,the results showed that the serum level of CTGF in the CHD groups were positively correlated with the pulmonary artery pressure (r =0.670,P =0.005 ; r =0.514.P =0.029 ; r =0.517,P =0.034 ; r =0.707,P =0.003).The difference in terms of serum CTGF concentration in different types of CHD patients was not significant (F =0.270,P =0.847).Conclusions There exists certain damage in CHD early myocardial fibrosis(unincorporated PH),when combined with PH,myocardial fibrosis increases with the gradual increase of pulmonary artery pressure.However,the degree of myocardial fibrosis shows no correlation with the type of CHD.