Objective To evaluate the performance of a latex enhanced immunoturbidimetric?2-microglobulin kit in clinical use. Methods The lower limit of detection,precision,accuracy and linearity range was determined on the automatic chemistry analyzer Hitachi 7170S. Results The lower limit of detection was 0. 09mg/L. The within-run precision was less than 4% while the between-run precision was less than 8%. The accuracy was 99.29 % and the linearity range was 0.2～18mg/L. Conclusion The?2-microgiobulin kit shows good performance in clinical use.

T-cell acute lymphoblastic leukemia (T-ALL) is a highly aggressive hematologic malignancy associated with poor prognosis.Increasing data regarding to alteration of gene expression signatures of oncogenes and tumor suppressors involved in the pathogenesis of T-ALL and the major mechanisms of T-cell transformation may contribute to define the biological markers for treatment response and prognosis,and has important clinical implications.In this review,advance knowledge concerning the characteristics of early T-cell precursor ALL,the alteration of TAL1 and NOTCH1 related genes and target inhibiton effects based on these alterations from 2012 the 54th ASH annual meeting ars summarized.

Angioplasty, Balloon, Coronary ; Humans ; Myocardial Infarction ; therapy

Objective To investigate the association between malnutrition parameters and serum N-terminal pro-brain natriuretic peptide (NT-proBNP) level in male patients aged ≥ 80 years with normal ejection fraction.Methods A total of 197 elderly male patients aged 80 95 years (average 85.4±9.7) were enrolled.Each patient received echocardiograms following admission,and ejection fraction was calculated.Serum concentrations of NT-proBNP were measured by electrochemiluminescence immunoassay.Multiple Logistic regression analyses and receiver operating characteristic (ROC) curves were used to assess the association between malnutrition parameters and serum level of NT-proBNP.Results Serum NT-proBNP level was higher in patients with malnutrition than in the controls [(711.9±1063.3) ng/L vs.(1375.2-±1891.7) ng/L,P=0.006].Pearson correlation analysis showed that the malnutrition parameters such as body mass index (BMI),blood hemoglobin,albumin and pre-albumin were negatively correlated with serum NT proBNP level (all P＜0.05).Logistic regression analysis showed that BMI and serum pre albumin level were independently associated with serum NT-proBNP level elevation (P=0.028 and 0.006,respectively).ROC curve analysis showed that the malnutrition parameters could predict the serum NT-proBNP level elevation.The area under the ROC curves for BMI,levels of blood hemoglobin,albumin and prealbumin in predicting serum NT proBNP level elevation was 0.7 (P=0.002),0.7 (P=0.004),0.6 (P=0.036),0.6 P=0.002),the sensitivity was 75.5％,51.0％,38.8％,89.8％,and the specificity was 54.4％,70.2 ％,84.2％ and 31.6 ％,respectively.Conclusions The malnutrition parameters including BMI,blood hemoglobin,albumin and pre-albumin can predict serum NT-proBNP level elevation.BMI and serum pre-albumin level are independently associated with serum NT-proBNP level elevation in male patients aged ≥ 80 years with normal ejection fraction.

Objective To reconstruct the soft tissue defect in the back or neck after excision of hypertrophoc scar or giant nevus by using transmidline pre expanding scapula flap vascularied by ipsilateral circumflex scapular artery. Methods The reconstruction can be accomplished in two stage procedures. First, scapular flaps were expanded for 4 6 weeks prior to transfer in 8 patients. Second,the pedicled flaps were designed across the entire back to incorporate both scapular territories but were rotated on a single vascular pedicle. Results All flaps survived. Only two flaps had marginal necrosis, and the wound healed well by dressing. Conclusion Flap expansion produces a delay that augments blood supply and increases the area of skin that survives on a single vascular pedicle. This technique may be useful in selected patients in whom a large and thin fasciocutaneous flap is required and there is sufficient time to allow flap expansion prior to thansfer. It is easy to harvest, and donor site morbidity is negligible. The patients can get better functions and quality of life.

The authors stated post-operative adjuvant therapy, combined chemotherapy, and palliative treatment for colorectal cancer, trying to summarize progress of the treatment of colorectal cancer with integrated traditional Chinese and western medicine, researching the mechanism of action. The authors also presented their arguments.

Recently a number of acupuncture clinical trial projects mainly conducted by conventional scientists have generated many negative results. A large meta-analysis of patient-level acupuncture data for the treatment of chronic pain conditions have demonstrated that the effects of verum acupuncture on pain improvement have statistically significant, but small, differences compared with sham-acupuncture procedures and no difference between acupuncture points and non-points. These conclusions have puzzled the acupuncture community and made confusion for acupuncture research and practices. The purpose of this paper was to compare differences between acupuncture clinical practices and the trial studies, which include "acupuncture technical principles", "acupuncture clinical trial design", and "acupuncture practice based on the theory of traditional Chinese medicine". These factors contribute to the puzzle between the acupuncture community/practice and acupuncture clinical trials, which can be improved in future studies.
Acupuncture Points ; Acupuncture Therapy ; Chronic Disease ; Clinical Trials as Topic ; Humans ; Meta-Analysis as Topic ; Pain Management ; methods ; Research Design

Amino Acids ; metabolism ; Animals ; Brain ; metabolism ; Electromagnetic Fields ; Electrophysiological Phenomena ; Glutamates ; metabolism ; Male ; Neurotransmitter Agents ; metabolism ; Pain ; physiopathology ; Pain Threshold ; Rats ; Rats, Sprague-Dawley ; gamma-Aminobutyric Acid ; metabolism

Objective To evaluate the laparoscopic ovarian cystectomy of benign lesions of security,superiority.Methods Laparoscopic ovarian cystectomy of benign lesions of security,superiority.Results The operation time of laparoscopic group (40.5 ± 5.3) min,significantly shorter than the control group (60.7 ± 6.8) min (t =-14.815,P =0.000) ; Bleeding (55.1 ± 9.8)ml was significantly less than that in the control group (90.0 ±15.0) ml (t =-18.275,P =0.001) ; Anus exhaust time (13.5 ± 8.3) hour was significantly shorter than that in control group (24.5 ± 9.6) hour (t =-4.351,P =0.000).The average postoperative hospital stay was (3.5 ±1.3) days was significantly shorter than that in control group (5 ± 2) days (t =-2.158,P =0.000).Postoperative morbidity was not statistically different from controls (t =0,5,P =0.053).Postoperative pain was significantly lower than that in control group (x2 =8.525,P =0.002).Conversion to open operation in laparoscopic group:2 cases,postoperative analgesic drugs were not needed,the postoperative 12h after catheter removal started eating and ambulation,postoperative shoulder pain in 2 cases ; open operation group after all need to use analgesics,postoperative 24h after catheter removal started eating and ambulation,abdominal incision fat liquefaction and delayed healing in 4 cases.The two groups had no visceral injury,postoperative bleeding,and chemical peritonitis.Conclusion Laparoscopy in the treatment of benign ovarian tumor is safe,less bleeding,rapid recovery,fewer complications and other characteristics.