AIM: To investigate the expression of aquaporins-1 (AQP-1)in cultured bovine corneal endothelial cells and to explore the role of AQP-1 in corneal endothelial fluid transport.METHODS:The bovine corneal cells were cultured in DMEM containing 200mL/L neonate bovine serum. AQP-1 expression in the bovine corneal endothelial cells was detected with immunohistochemistry method before and after treatment of the cells with aquaporin inhibitor, p-chloromercuribenzene sulfonate. The osmotic water permeability was determined bymonitoring volume changes of cultured bovine corneal endothelial cells.RESULTS: AQP-1 expression in the membrane of cultured bovine corneal endothelial cells was revealed by positive staining (in brown color). The reading of osmotic water permeability of the cultured bovine corneal endothelial cells before treatment with p-chloromercuribenzene sulfonate was 0.044±0.005cm/s, which significantly decreased to 0.017 0.003 cm/s(n= 15) after treatment.CONCLUSION: AQP-1 expressed in the membrane of cultured bovine corneal endothelial cells may play an important role in fluid transport of corneal endothelial cells. Alteration of the AQP-1 expression may cause abnormal corneal function and corneal edema.

OBJECTIVE:To explore the general rules and characteristics of ADR/ADE induced by Glycerol fructose injection and provide reference for clinical ration drug use. METHODS:284 cases of ADR/ADE induced by Glycerol fructose injection from Jan. 2004 to Oct. 2014 in Drug ADR Monitoring Center of Henan Province were statistically analyzed and comparatively analyzed with the drug instructions in 11 domestic drug manufacturing enterprises. RESULTS:ADR/ADE induced by Glycerol fructose injec-tion was related to gender,mainly adult male,and ADR/ADE might occurred within 30 min;main clinical manifestations were skin and its appendages damages,medication site damage and systemic damage. There were larger differences among the notes de-scription in the package inserts from 11 drug manufact urers. CONCLUSIONS:The clinic should strengthen the monitoring of ADR/ADE induced by Glycerol fructose injection,try to avoid or reduce the incidence of ADR,improve the drug instructions and ensure medication safety.

[Objective]To summarize the professor WU Lianzhong's experience in treatment of spasmodic torticollis and myelitis sequela by using acupuncture,so as to guide clinical practice better.[Methods] From following professor WU Lianzhong clinical studies,studying professor WU Lianzhong's medical cases,introducing Professor WU to treat spasmodic torticollis with Du channel four points and five points and treat myelitis sequela by using acupuncture and traditional Chinese medicine,we can summarize his academic thoughts and experience on treating spasmodic torticollis and myelitis sequela.[Results] Professor WU thinks the key pathogenesis of Du channel type spastic torticollis is Orifices blocked and consciousness delirium,so the treatment principle is to clear the heart and reduce the fire,and regulate consciousness,at the same time strengthen the Du channel and break the nodules,this method is effective in clinical treatment.He holds that the etiology and pathogenesis of myelitis mainly for lack of spirit,attack of exogenous pathogens,prolonged illness lead to deficiency of liver and kidney,qi and blood deficiency,all these lead to tendon and vessel malnutrition.The cardinal treatment principle is to nourish liver and kidney,tonify qi and blood,dredge the channels and collaterals,also comprehensivey treat this disease by acupuncture combined with traditional Chinese medicine,the clinical effect is satisfying.[Conclusion] Professor WU's experience in treatment of Du channel type spasmodic torticollis and myelitis sequela is worth studying and popularizing in clinic.

Hepatitis B virus infection can result in different clinical outcomes which consist of self-limited infection(virus clearance),asymtomatic carrier status,chronic hepatitis B and cirrhosis.The mechanism led to individual discrepancy for outcome of hepatitis B virus infection is unknown yet.It is suspected that the outcomes of hepatitis B virus infection might be associated with the inherent factors of host.The results of association studies between genetic polymorphisms of HLA-I,Ⅱ class,TNF-? and VDR and different clinical outcome of hepatitis B virus infection in recent years are reviewed.

Objective To explore the whole constructive conception and organization structure strategy of multi-disciplinary team (MDT) for colorectal cancer (CRC). Methods Combined the characteristics of large public hospital, with recognized treatment pathway in MDT for CRC and the way of medical project construction, MDT for CRC project team summarized a system of MDT for CRC of West China Hospital (MDT-CRC-WCH) by own characteristics and subject feature. Results MDT for CRC summarized the 5 basic characteristics about profession, classification, interaction, optimization and fast. The project has the core competencies: system new operation types for colorectal cancer and volunteer culture. By the matrix organization structure, MDT set the main departments: database team, follow-up team, nursing team and public team. Conclusion With effective MDT whole construction and suitable organization structure, MDT will develop in long time.

Objective The article explained how to build the data system and its running strategy in the mode of multi-disciplinary team (MDT) for colorectal carcinoma. Methods It illuminated the cause of the data system building, also described the essential composition of the data system and how to support the running of the data system, and it discussed the value feedback of the data system, lastly the author proposed the prospect of the data system building. Results The data system could work normally through consultation of doctors, follow-up, clinical support and appropriate implement of construction of information flow-sheet in colorectal carcinoma MDT mode. Conclusion As the foundation of colorectal carcinoma MDT, data system could show both medical and social value through change of medical mode.

Objective To report the progression of breviscapine's protective effect to hepatic ischemia-reperfusion injury.Methods Pertinent literatures and journal articles published in recent years were reviewed,and the progression of breviscapine protecting hepatic ischemia-reperfusion injury in the experimental and clinical research were analyzed and summarized.Results The role of breviscapine is considerable extensive.It can protect hepatic ischemia-reperfusion injury by anti-oxyradical and anti-lipid peroxidation,inhibiting mitochondrial damage,intracellular calcium overload,intra-thromboxane and apoptosis,improving microcirculation,and so on.Conclusion Breviscapine plays a protective role in hepatic ischemia-reperfusion injury,and it will be of great value to application and research.

Objective: To study the effect of EEG biofeedback in the treatment of children with ADHD and comorbid tic disorder. Methods:14 children with ADHD and comorbid tic (criteria of DSM-IV) aged 7 to 14 received EEG biofeedback treatment (average 34 sessions). The outcome was evaluated with Conners Parent Symptom Questionnaire(PSQ),Rutter Questionnaire,Achenbach CBCL,and C-WISC-R,WMS,CPT before and after treatment. YGTSS was used to evaluate the severity of tic disorder. Results: Hyperactivity index in PSQ, the total score of Rutter, and hyperactivity factor in CBCL were greatly reduced after treatment (P

Internet not only substantially improve the efficiency of work, but also realize the sharing of information resources to the broadest extend. At present, China has the world's largest number of Internet users and the large basis network, a very serious problem is that Internet security issues. The issue of the status on network security of domestic and abroad is discussed and the related recommendations of internet security are putted forward.

Birds,especially wild waterfowls,are a natural reservoir of influenza virus,though poultry,mammalians and even human beings are also thought to be the potential hosts.Influenza virus can be classified into subtypes according to the characteristics of the nucleocapsid protein and membrane protein,each subtype with its own most adaptable host,so called host range restriction.However,the outbreak and spreading of influenza in different species has aroused increasing attention since its disastrous consequence on the ecological system of the earth.Large-scale epidemic survey,prediction of possible mutation of the virus,development and application of vaccines are key actions to be taken to prevent the pandemic of influenza.This article offers an overview of the ecology of influenza virus,features of recent great outbreaks of avian influenza,and relevant issues of concern.