Using observation of the gross and histological changes of skin, intestine, liver and spleen, the spleen index, the popliteal lymph node weight gaining, and the ~(125)I-UdRincorporation assay, the effects of GvHR in the pathogenesis of immunologically mediatedaplastic anemia in mice were studied. Rtsults showed that there were no gross and histological changes due to GvHR foundin skin, intestine, liver and spleen, and the spleen index decreased markedly, and therewere positive popliteal lymph node weight gaining and positive ~(125)I-UdR incorporationassay which showed a dose dependent corrlation. These results suggested that, transfusion of DBA/2 lymphocytes into lethally irradia-ted BALB/c mice could evoke GvHR, but on the other hand, the effects of GvHR werenot very important in the pathogenesis of immunologically mediated aplastic anemia inmice.

Animals ; Body Water ; Brain ; anatomy & histology ; Male ; Organ Size ; Rats ; Rats, Wistar ; Specific Gravity

Objective To investigate the expressions of STAT3 and HIF-1α in esophageal squamous cell carcinoma (ESCC) and its relationships with clinical pathological characteristics and prognosis. Methods The expressions of STAT3 and HIF-1α protein were examined via immunohistochemistry in 50 cases of ESCC and normal esophageal mucosa tissue. Results In the immunohistochemistry of 50 ESCC , the positive expression rates of STAT3 and HIF-1α were 70% and 58%, respectively , significantly higher than those at the adjacent normal mucosa (P < 0.05). The expression of STAT3 in ESCC patients was significantly related to the lymph node metastasis, depth of invasion and TNM stage. The 30-month survival rates were positively correlated with the positive expression of STAT3 (P < 0.05). The expression of HIF-1α in ESCC patients was significantly related to the degree of differentiation and lymph node metastasis. The patients with HIF-1α negative had a higher cumulative survival rate than those with HIF-1α positive (P < 0.05), but the difference was not significant. STAT3 and HIF-1α were positively correlation with statistical significance (r = 0.362,P < 0.05). Conclusion STAT3 and HIF-1α are frequently expressed in ESCC. High expressions of STAT3 and HIF-1αmay contribute to the poor prognosis of human esophageal squamous cell carcinoma.

Background and purpose: Unbalance of apoptosis regulation plays an important role in tumor initiation and progression. Survivin and caspase-3 all take part in the regulation of cell cycle and apoptosis. The expressions of survivin and caspase-3 were detected in the tissues of gastric cancer to explore their association with the carcinogenesis of gastric cancer. Methods: The expressions of survivin and caspase-3 in 79 cases of gastric cancer and 30 cases of adjacent were detected by using reverse-transcription PCR (RT-PCR) and immunohistochemistry. Results: The expressions of survivin mRNA and caspase-3 mRNA were significantly related with the depth of invasion, lymph-node metastasis and TNM stage (P<0.05). The expressions of survivin mRNA and caspase-3 mRNA had significantly differences in gastric cancer (P<0.01). Conclusion: Survivin and caspase-3 are both important factors that cause tumorigenesis of gastric cancer, while survivin may promote the progression of gastric cancer through inhibiting the expression of caspase-3.

Objective To investigate effects of Kanglaite injection on proliferation, cycle and apoptosis of human breast cancer MCF-7 cells;To discuss its relevant mechanism. Methods Logarithmic growth phase cells were divided into control group and Kanglaite-treatment group (10, 20, 40μL/mL). Cells were cultured in RPMI-1640 for 24 h before drug treatment. The inhibition rate of Kanglaite injection on proliferation of human breast cancer MCF-7 cells was detected by MTT assay. Apoptosis and cell cycle of MCF-7 cells were detected by flow cytometry. Changes in cell nucleus were determined by Hochest staining assay. Protein expressions of Bcl-2 and Bax were detected by ELISA and Western blot. Results Kanglaite injection for 12 h, 24 h or 48 h resulted in a significant inhibition of MCF-7 cells proliferation (P<0.05, P<0.01);Compared with the control group, Kanglaite injection-treated cells showed increased percentage in G2/M and G0/G1 phases (P<0.001, P<0.01), but showed decreased percentage in S phase (P<0.01), and apoptosis rate increased (P<0.05, P<0.001). Kanglaite injection significantly decreased protein expression of Bcl-2, and enhanced protein expression of Bax of MCF-7 cells (P<0.01, P<0.001). Conclusion Kanglaite injection can inhibit the proliferation of human breast cancer MCF-7 cells, decrease cell cycle and induce apoptosis, the mechanism is related with decreasing protein expression of Bcl-2 and enhance the protein expression of Bax.

Objective To investigate the role of T follicular helper ( Tfh) cells in the immuno-pathogenesis of persistent immune thrombocytopenic purpura ( pITP) .Methods Twenty children with pITP and twenty healthy controls were enrolled in this study .The proportion of CD4+CXCR5+ICOShigh PD-1high T ( cTfh) cells and the expression of ICOSL on CD 19+B cells in peripheral blood of the patients and healthy subjects were analyzed by flow cytometry .The expressions of Bcl-6, c-Maf, IL-21 and ICOSL at mRNA level were detected by real-time PCR.The plasma concentrations of IL-2, IL-6 and IL-21 were determined by ELISA.Results (1) Compared with the healthy controls , the proportions of cTfh cells increased signifi-cantly in patients with pITP [(17.45±9.04) %vs.(7.57±2.57) %, P<0.05], but decreased with the treatment of dexamethasone (DEX) for 7 days [(5.93±1.64) %vs.(17.45±9.04) %, P<0.05].(2) The expression of Bcl-6, c-Maf and IL-21 at mRNA level in patients with pITP were higher than those in healthy controls (P<0.05).(3) Compared with healthy controls, the expression of ICOSL at mRNA and protein levels in CD19+B cells were significantly up-regulated in patients with pITP (P<0.05), which showed no significant changes after treatment with DEX (P>0.05).(4) The plasma concentration of IL-21 was remarkably elevated in patients with pITP , regardless of DEX treatment (P<0.05).But the plasma concentrations of IL-2 and IL-6 showed no significant changes compared with control group (P>0.05).Con-clusion The immunopathogenesis of persistent immune thrombocytopenic purpura might be associated with the hyper-activation of Tfh cells caused by excessive expression of ICOSL and IL-21.The persistent high expression of ICOSL and IL-21 might be one of the important factors resulting in the recurrence of pITP in children .

Objective To compare the effectiveness and the influence on life quality of endovenous laser obliteration with those of traditional surgical ligation in treating varicosis of lower extremity.Methods By using numerical random grouping method,40 patients with varicosis of lower extremity were divided into traditional surgical ligation group (high ligation plus stripping,traditional group,n=20) and endovenous laser obliteration group (laser group,n=20).The curative effect and complications were compared between the two groups.According to the brief health survey (SF-36) scale,the influences of the two treatments on the life quality were evaluated.Results During the follow-up period,the differences in the curative effect and complications between two groups were not significant (P＞0.05).The life quality assessed by SF-36 scores in the laser group was better than that in the traditional group,the difference between the two groups was statistically significant (P＜0.05).Conclusion For the treatment of varicosis of lower extremity,endovenous laser obliteration is safe and effective,it carries less influence on the patient's life quality when compared with traditional surgical ligation therapy.

Objective To study the relationship between FAB‐classifying standard and immunophenotype of leukemia in clinical diagnostic and treatment .Methods There were 462 cases of leukemia according to FAB‐classifying standard and 34 cases by immu‐nophenotype in our hospital from 2011 to 2013 .The results of FAB‐classifying standard and immunophenotype were statisticed and compared .Results Among the 462 cases ,there were 171 acute myeloid leukemia (AML) ,50 acute lymphoblastic leukemia (ALL) , 76 chronic leukemia ,3 rare types ,56 unclear types and 27 mismatched according the FAB‐classifying standard .The diagnostic rate was 82 .03% .34 cases of immunophenotype included 29 single phenotype ,4 double phenotypes and 1 unknown .The diagnosis rate was 97 .06% .CD13 and CD33 have high positive express in AML ;CD34 and HLA‐DR do not express in M3 ;CD7 which was known as a lymphatic antigen also was founded in AML .Conclusion The combing use of FAB‐classifying standard and immunophenotype can improve diagnostic rate ,and immunophenotype has an important role in differentiating different subtypes of leukemia .

Objective To study the effect of cypermethrine (CP) on the reproductive organs of female rats.Methods Fifty female SD rats (90-110 g) were randomly divided into 5 groups,negative control (earthnut oil),three CP treatment groups(20,40 and 80 mg/kg body) and positive control (E_2 100?g/kg body),treated by gavage,once a day,for 28 consecutive days.Results Compared with the negative group,the opening of vagina in different cypermethrine groups were significantly moved up in the first week (P

AlM: To analyze, summarize and describe ophthalmic imaging features of posterior scleritis.
METHODS: Clinical data of 16 patients ( 21 eyes ) with posterior scleritis diagnosed in our hospital from October 2008 to June 2013 were retrospectively analyzed. The results of type-B ultrasonic, fundus chromophotograph, fundus fluorescein angiography, CT were recorded for comprehensive evaluation and analysis of ophthalmic imaging features of posterior scleritis.
RESULTS: All patients underwent type-B ultrasonic examination and manifested as diffuse and nodular types. The diffuse type showed diffusely thickened sclera and a dark hypoechoic area that connected with the optic nerve to form a typical “T”-shaped sign. The nodular type showed scleral echogenic nodules and relatively regular internal structure. FFA showed that relatively weak mottled fluorescences were visible in the arterial early phase and strong multiple needle-like fluorescences were visible in the arteriovenous phase, which were then progressively larger and fused; fluorescein was leaked to the subretinal tissue in the late phase;varying degrees of strong fluorescences with less clear or unclear boundaries were visible in the optic disk. CT results showed thickened eyeball wall.
CONCLUSlON: Posterior scleritis is common in young female patients, whose ophthalmic imaging features are varied and more specific in type-B ultrasonic. Selection of rational ophthalmic imaging examination method, combined with clinical manifestations, can accurately diagnose posterior scleritis and avoid the incidence of missed and delayed diagnosis.