The Wnt/β-catenin signal transduction pathway is essential for regulating cell proliferation, movement, differentiation and embryo development. Researchers have found that Wnt pathway is an important regulation factor in the early stimulation of the cardiogenesis and contacts closely the several key events during cardiac morphogenesis. The article reviews the recent studies about the relation between cardiac development and Wnt pathway in early embryo.

The Wnt/?-catenin signal transduction pathway is essential for regulating cell proliferation,movement,differentiation and embryo development. Researchers have found that Wnt pathway is an important regulation factor in the early stimulation of the cardiogenesis and contacts closely the several key events during cardiac morphogenesis. The article reviews the recent studies about the relation between cardiac development and Wnt pathway in early embryo.

Aim To investigate the effects of curcumin on expression of HIF-1? in human hepatocellular carcinoma BEL-7402 cells.Methods Different concentrations of 0,2.5,5,10,15 and 20 ?mol?L~(-1) curcumin-treated human hepatocellular carcinoma BEL-7402 cells were cultured under hypoxia for 6 hours.The cell proliferation and cell viability were assayed by WST-8 and the trypan blue dye exclusion method.The expression level of HIF-1? in human hepatocellular carcinoma BEL-7402 cells was checked by RT-PCR and Western Blot;0,10 ?mol?L~(-1) curcumin,10 ?mol?L~(-1) MG-132,and 10 ?mol?L~(-1) curcumin +10 ?mol? L~(-1) MG-132-treated human hepatocellular carcinoma BEL-7402 cells were cultured under hypoxia for 6 hours.The protein expression level of HIF-1? protein in human hepatocellular carcinoma BEL-7402 cells was checked by Western Blot.Results ① No significant difference was found in the cell proliferation rate and cell viability between control and different concentrations of curcumin.② Curcumin can down-regulate the expression of HIF-1? protein with the increasing concentrations.③ the effect of curcumin on expression of HIF-1?mRNA had no significant difference between control and different concentrations.④ MG-132 can reverse the curcumin-mediated decrease of HIF-1? protein.Conclusion Inhibitory effect of curcumin on expression of HIF-1? in human hepatocellular carcinoma BEL-7402 cells was acted through posttranscriptional mechanisms and the proteasome pathway.

As part of medical scientific spirits and closely associated with medical ethics,medical rational spirit is the spiritual foundation of medical treatment activities and thus should evolve compatibly with medical science.Due to the specific characteristics of medical science,a noble and steady medical rational spirit is an important factor in accelerating harmonious medical consumption and basic request for the application of logic inevitability of medical purpose.

That sparkplugs and cognizances caution of medical morality base line is a realistic proposition that to spurn mammonism phenomenon filters medical service industry and enhances diathesis of medical affairs personnel.Medical service incited by mammonism phenomenon forms a menace to the medical base line,it is important inducement of medical action of getting out of line,and duties of medical risk and complaints of medical comsumer.Mammonism phenomenon form a nubbin quoted by medical morality base line and duties of medical risk.

Animals ; Cells ; metabolism ; radiation effects ; Electromagnetic Fields ; Humans ; Reactive Oxygen Species ; metabolism

Objective To investigate the intelligence level and characters in children with cerebral palsy of 4~6 years old, and the factors related with their intelligence. Methods 215 cerebral palsy children of 4~6 years old were assessed with Wechsler Intelligence Scale for children,Gross Motor Function Classification System (GMFCS). Results 210 children finished their intelligence test, and 113 presented mental retardation. The intelligence level was higher in the children with spastic diaplegia, hemiplegia and dyskinetic type than those with quadriplegia and ataxia type. The difference was significant between VIQ and PIQ in the spastic diplegia (P<0.05). The mental retardation was more frequent in the children with GMFCS Ⅳ~Ⅴ, or with epilepsy or microcephaly than those with GMFCS Ⅰ~Ⅲ or without the complications (P<0.05). Conclusion The incidence of mental retardation in children with cerebral palsy was related with the neurotypes, motor function and complications.

ObjectiveTo investigate the cause, treatment and prevention of splenic injury in radical nephrectomy for left renal cell carcinoma.MethodsThe clinical data of 27 cases of splenic injury in 458 cases of radical nephrectomy for renal cell carcinoma were retrospectively analyzed.ResultsThe injured spleen was saved in 21 cases (18 gradeⅠ injury,3 grade Ⅱ).The injured spleen was sewed up and pressed with hemostatic ganze in 7,pressed with medical sponge laid with spurting argon on biological glue in 11 and the same was carried out in 3 grade Ⅱ injury.Spleenectomy was conducted in the other 6 cases (4 grade Ⅱ injury,2 grade Ⅲ).The patients have been followed up for 6 months to 5 years.The outcome has been good in all except 1 patient died of tumor recurrence 1 month after operation.No late bleeding or adverse side effects has been noted.Conclusionssplenic injury may occur in radical nephrectomy for renal cell carcinoma especially the tumor is lasge or adhered to spleen in the upper past of left kidney.The occurrence of splenic injury is also related to the tumor stage.Attention should be called to the mentioned surgical complication.

[Objective]To investigate the possibility of using poly(hydroxybutyrate-co-hydroxyvalerate)(PHBV)as scaffolds for cartilage tissue engineering and to compare the engineered cartilage generated in vitro with those in nude mice modles.[Method]PHBV porous scaffolds were fabricated using a compression moulding,thermal processing and salt particulate leaching methods.Chondrocytes isolated from articular cartilage were seeded into porous PHBV scaffolds.After incubation for 2 weeks in vitro,chondrocytes-PHBV constructs were implanted subcutaneously in the dorsum of athymic nude mice.Control groups were established by subcutaneous implantation of PHBV alone.The implants harvested after in vivo incubation of 4 and 8 weeks and cell-scaffolds cultured in vitro for 6 and 10 weeks were respectively examined histologically.Chondrocytes cultured for 3,7 and 14 days on the scaffolds were examined by SEM.[Result]SEM showed that chondrocytes could aggregate and synthesize extracellular matrix on PHBV scaffolds.Both specimens harvested from nude mice and those cultured in vitro demonstrated new cartilage formation,while characteristics of the engineered cartilage generated in vivo were more typical.Those from control groups showed no cartilage formation.[Conclusion]PHBV can be used as scaffolds for cartilage tissue engineering and nude mice modle seems to facilitate chondrogenesis compared with that in vitro system.

Objective To establish an effective cleaning process for automatic spray cleaning machine in case of without water purification system and to reduce the formation of scale on the surface of machine and instruments with increased cleaning degree.Methods Descale process was added to the previons concrete cleaning process.The clean effect Was compared with previous concrete process.The blocking of the pipeline was observed.Results The clean degree by new cleaning process reached 97.23%,which was,better than that of the previous process.The problem of blocking of the pipeline was also solved.Conclusions Addition of descale process when using automatic spray cleaning machine in case of without water purification system could guarantee the clean quality of instruments.