ItisacknowIedgedthatintestinaImicrobiotapIaysanimportantroIeinIifeprocess.IntestinaIdysbiosisis invoIved in the pathogenesis of many diseases reIated to aImost every system of the body. Probiotics and fecaI microbiota transpIantation,which can moduIate intestinaI microbiota without severe adverse effects,has become a hot topic for disease treatment. This articIe summarized the use of intestinaI microbiota moduIation for treatment of digestive system diseases.

Objective: To investigate the expression of Bcl-2 and Bax in renal tissues of patients with hepatitis B virus-associated glomerulonephritis (HBV-GN). Methods: Twenty HBV-GN specimens with complete nephrology data and 10 normal renal specimens were randomly chosen for the present study. Cell apoptosis was detected by means of terminal deoxynucleotidyl transferase mediated d-UTP nick end labeling (TUNEL) and the apoptotic index was calculated; immunohistochemistry was used to detect the protein expression of Bax and Bcl-2. Results: The apoptotic index in HBV-GN group was obviously higher than that of the control group; the apoptotic cells were mainly distributed in the proximal and distal renal tubules and the collecting duct epithelial cells, seldom seen in the glomerular cells. The expression of Bcl-2 in HBV-GN patients was predominately present in the renal tubular epithelia cells (positive in the plasma, membrane and nuclear); the expression of Bax was found in both glomerular cells and renal tubular cells, mainly in tubular epithelial cells, seldom seen in Bowman's capsule or glomerular mesangial region. Conclusion: Apoptosis in the kidney of HBV-GN patients mainly occurs in the renal tubular epithelial cells; expression of Bax and Bcl-2 is mainly in the renal tubular epithelial cells, suggesting that the injury of tubular interstitial damage may be one of the important factors for the development of HBV-GN.

The mechanism of long chain non-coding RNA (lncRNA) is diverse. LncRNA can regulate the expression of genes at multiple levels and is closely related to some pathological process, such as tumor formation, viral replication, inflammatory damage, etc. The abnormal expression of lncRNA is involved in the patho-physiological changes of the disease, and plays a key role in many links, such as the stability of mRNA, the regulation of translation level, protein transport, RNA processing and modification, and so on. The development of sepsis inflammatory injury is a complex process involving multiple genes and pathways, in which the abnormal structure and expression of many encoding genes or non-coding genes exist.This paper review the research progress on lncRNA in the regulation of chondriokinesis, pro-inflammatory cytokines, calcium ion transport, nuclear factor-κB (NF-κB) in signal pathway and other inflammatory lesions, which may provide a new method for the diagnosis and treatment of sepsis.

Objective To explore the effects of cryopreservation on biological characteristics of cultured limbal stem cells.Design Experimental study.Participants Twenty New Zealand white rabbits.Methods Twenty New Zealand white rabbits(40 eyes) were preparated of peripheral cornea with 2 mm and 2 mm circular shallow conjunctival limbal graft.Rabbit limbal stem cells(Rb-LSCs) used for cell-suspention culture were isolated form New Zealand white rabbit limbal tissues.The limbal stem cells,having formated monolayer cultured limbal stem cells,were cryopreservated one month,then thawing.By immunofluorescence staining,flow cytometry,inverted microscope,MTT assay,we compared the subculture of limbal stem cells cryopreservation before and after the structure and properties.Main Outcome Measurements Limbal stem cell activity and biological characteristics.Results Compared with freshing cells,the cells after cryopreservation in morphology were poor adherence late,small nuclear cytoplasm ratio,irregular shape.Im munofluorescence staining to detect the low-temperature frozen cells ABCG2 monoclonal antibody 81.7% positive rate decreased to 46.9%,the difference was statistically significant(P

Objective To study whether a lactose-free,hydrolyzed protein formula would ameliorate feeding intolerance and weight gain in very low birth weight infants.Methods The study was conducted on very low birth weight infants ( gestational age ≤34 weeks, birth weight ≤1500 g) in our NICU from August 2010 to August 2013. According to the number of admission, the cases were assigned into lactose-free hydrolyzed protein formula group ( hydrolyzed protein group) and preterm formula group. The data of basic information ( gestational age, birth weight, way of labor, sex, asphyxia ), commencement of feeding time ( days) , enteral caloric intake, parenteral nutrition caloric intake, The time ( days) required to regain birth weight, weight gain index, days of parenteral nutrition, prealbumin level, feeding intolerance, the incidence of necrotizing enterocolitis ( NEC ) and extrauterine growth retardation(EUGR)werecollected.Results A total of 83 infants were recruited in the study. 40 infants were in hydrolyzed protein group and 43 infants were in preterm formula group. There were no differences between groups for basic information ( P>0. 05). The weight gain index of hydrolyzed protein group was higher than that of preterm formula group (13. 5 ± 2. 4 g/d vs. 11. 9 ± 3. 7 g/d, P=0. 036). There were less cases of feeding intolerance in hydrolyzed protein group than in preterm formula group & nbsp;(28/40 vs. 38/43, P=0. 038). The differences of the incidence of NEC (1/40 vs. 2/43) and EUGR (21/40vs.25/43)showed no significance statistically.Conclusions Lactose-free hydrolyzed protein formula can obviously improve feeding intolerance and early weight gain of premature infants.

Many malfunctions may happen in the operation of plasma sterilization machine.The basic principle and running procedure of plasma sterilization system under normal condition are introduced,and the causes of malfunctions are mainly analyzed so that users can discover and obviate them immediately.

Objective To observe the relationship between the levels of plasma VEGF,PAI-1 and the severity of the diabetic retinopathy in type 2 diabetic patients.Methods ELISA method was used to measure the levels of VEGF and PAI-1.Results The level of VEGF was increased in all diabetic patients compared with the normal control group[(17.86?12.25)ng/L],and that of the patients with BDR[(93.41?54.69)ng/L]was higher than that of the patients with NDR[(52.17?21.81)ng/L]and PDR[(61.24?37.55)ng/L].The level of PAI-1 was also increased in all diabetic patients.The increase tendency was more obvious in patients with BDR[(58.29?20.53)?g/L]and PDR[(66.84?23.81)?g/L].Conclusion The damage of the vascular endothelial cell and the impaired fibrinolysis state exist in diabetic retinopathy.So it is very important to measure the levels of VEGF and PAI-1 for the early diagnosis and intervention treatment of diabetic retinopathy.

Objective To study the diagnostic value of cavernosography in the venous leakage impotence.Methods Cavernosography were performed for 25 patients with the venous leakage impotence who had been diagnosed by the clinic just after the contrast agent had been injected.The appearance of cavernosography were analysed.Results Different degree venous leakage had been shown in 25 cases.The diagnostic accordance rate of the cavernosography,clinic impotence for the venous leakage was 100%(25/25).The accordance rate of the degree of the venous leakage shown by the cavernosography and pointed out by the clinical impotence was 36%(9/25).Conclusion Cavernosography not only can show the venous leakage and its location,but also can know the degree of the venous leakage.

Objective To study occupational hazards effects of electric welding,and evaluate protective measurements for different occupational hazards. Methods During 2008,occupational health services for 11 factories with shield carbon-dioxide arc welding work were provided,including surveillance of the work environment and surveillance of workers’health. Surveys of workers’health through various types of health examinations were carried out. Four hundred and thirty-three welding workers of 11 factories were included. Results The concentrations of 17 welding fume samples and 6 manganese dioxide samples were over the released limits of national standard,the maximum was 5.5,1.6 times of the limit respectively. The exceeding standard rate. of welding fume had positive correlation with the density of welding equipment(r= 0.72,P=0.01) and negative correlation with the ventilation area(r =-0.63,P =0.04) .The examination results showed that nobody suffered occupational diseases or occupational contraindications. Conclusion For the environmental risk factors existed in the electric welding operation site,more attention should be paid to the potentially occupational adverse effects of electric welding.

Objective To know the primary effect of nutrition intervention in farmers. Methods The questionnaires were used to do baseline and evaluation survey. The intervention ways were used in trial group, including holding lectures, posting posters, sending out healthy material, quizzes with prizes, recommending salts low in sodium to each family, training to make healthy food by the chef; but there were no measures in control group. Results There were statistical significance in healthy awareness of nutrition, exercises and chronic diseases between two groups after intervention; However, the average intake amounts of vegetables did not reach the recommendation of diet guidelines; After intervention, the intake frequency of vegetables and fruits had statistical significance between before and after intervention in trial group, and also had statistical significance in two groups. Conclusion The awareness of healthy knowledge and attitudes has been obvious improvement, but the diets and life styles of farmers have no obvious change. The diet structure and behavior of farmers have been increasingly rational but not enough. The farmers have started to increase the intake times of vegetables and fruits and built up the conception of eating more vegetables and fruits.