BACKGROUND: The method and effect of construction of tissue-engineering heart valve scaffold with cell detergents are different, and the method of detergent combining with the trypsase and nucleese is more suitable than others. OBJECTIVE: To investigate the effect of different detergents (sodium deexycholate, sodium dodecylsulfate, and triton) combined with trypsase on decellularized porcine heart valve and the influence on the accellular scaffold. METHODS: Porcine aortic valve leaflets were sterilized by antibiotics for 12 hours, maintained in the solution of the trysin and the EDTA for 12 hours, and treated with sodium deexycholate, sodium dodecylsulfate, and triton. Finally, the sample was dip in nucleese solution for 12 hours to remove endothelial cells and interstitial cells. HE staining was used to detect whether the endothelial cells were removed completely, Masson staining was used to evaluate damage level of collagen fiber and elastic fiber, and electronic scanning was used to observe the microstructure. RESULTS AND CONCLUSION: All the three detergents completely removed the endothelial ceils; however, the effect of sodium decxycholate on collagen fiber and elastic fiber was light, and then sodium dodecylsulfate and triton. This suggested that the method of DOA combining with the enzyme digestion was a suitable technique to construct tissue-engineering heart valve scaffold.

Objective:To investigate the activated state of TH1/TH2 cytokines and T lymphocytes and to explore the pathogenesis of ankylosing sondylitis.Methods:Cytokine levels of Th1(IFN-?、TNF-?、IL-2)and Th2(IL-10、IL-5、IL-4) in plasma, percentages of CD3 +、CD4 +、CD8 + T cells、B cells(CD19 +) NK cells(CD16 +56 +)and CD3 +HLA-DR +、CD4 +HLA-DR +、CD8 +HLA-DR + T cells in peripheral blood lymphocytes were detected by Flow Cytometry.Results:In patients with AS ,plasma levels of TNF-?、IL-2 were significantly lower than that of healthy controls. IL-10 were significantly higher than that of healthy controls. In AS patients, percentages of CD3 +and CD8 + T cell from peripheral blood lymphocytes were significantly lower than that of healthy controls. Percentages of CD8 +HLA-DR + T cell were significantly lower than that of healthy controls. CD4 +HLA-DR +T cell was significantly highter than that of healthy controls.Conclusion:In patients with AS , lower levels of TNF-?、 IL-2 and higher level IL-10 at plasma indicate an inclination between TH1 and TH2, such as an impaired TH1 cytokine profile and an activated TH2 cytokine profile, especially in TNF-?.

Animals ; Female ; Hippocampus ; physiology ; Learning ; Long-Term Potentiation ; Male ; Memory ; Mice ; Mice, Inbred Strains ; Pregnancy ; Prenatal Exposure Delayed Effects ; psychology ; Tobacco Smoke Pollution

Endophytic fungi which reside in the tissue of mangrove plants seem to play an important role in the discovery of new biologically active substances. During the course of screening for the antimicrobial metabolites from the endophytic fugus Penicillium sp. FJ-1 of mangrove plant Avicennia marina, a new aurone glycoside (1) was isolated by repeated column chromatography on silica gel and recrystallization methods. The structure of 1 was elucidated as (Z)-7,4'-dimethoxy-6-hydroxy-aurone-4-O-β-glucopyranoside, on the basis of spectroscopic analysis. Compound 1 exhibited antifungal activity against Candida sp., with the potency comparable to amphotericin B and much better than fluconazole. Compound 1 can also inhibit extracellular phospholipase secretion in a concentration-dependent manner.
Antifungal Agents ; chemistry ; isolation & purification ; metabolism ; pharmacology ; Benzofurans ; chemistry ; isolation & purification ; metabolism ; pharmacology ; Candida ; drug effects ; Glycosides ; chemistry ; isolation & purification ; metabolism ; pharmacology ; Molecular Structure ; Penicillium ; chemistry ; genetics ; isolation & purification ; metabolism ; Seawater ; microbiology

Vasculogenic mimicry (VM) can promote tumor growth, invasion, and metastasis. The formation of VM is regulated by various proteins including epithelial cell kinase, phosphoinositide-3 kinase, and hypoxia-inducible factor 1. Local microenvironment also plays an important role in regulating VM formation.

To discuss the feasibility of the pharmacodynamic evaluation method for traditional Chinese medicine (TCM) formula particles, with traditional decoction for reference and the intervention of Magnoliae Officinalis Cortex in rats with ulcerative colitis (UC). First of all, the similarity of traditional Magnoliae Officinalis Cortex decoction and formula particles of different manufacturers was defined by using the IR fingerprint. The UC rat model was established and given Houpo formula particles of different doses and manufacturers, with the decoction for reference, in order to observe disease activity index (DAI), colon mucosa damage index (CMDI), pathologic changes, nitric oxide (NO), endothdin (ET), substance P, vasoactive intestinal peptide (VIP). Their intervention effects on UC rats were compared to study the difference between Sanjiu and Tianjiang Houpo formula particles, in order to demonstrate the feasibility of the pharmacodynamic evaluation method for Houpo formula particles. According to the results, Houpo formula particles showed similar pharmacodynamic actions with the traditional decoction. The pharmacodynamic comparison of Houpo formula particles of different manufacturers showed no statistical significance. The experiment showed that on the basis of the TCM compounds, a prescription dismantlement study was conducted to define target points of various drugs. The traditional decoction was selected for reference in the comparison of corresponding formula particles for their pharmacodynamic equivalence. This method could avoid controversies about single or combined boiling of formula particles, and give objective comments on the pharmacodynamic effect of the formula particles. The method is proved to be feasible.
Animals ; Chemistry, Pharmaceutical ; methods ; Colitis, Ulcerative ; drug therapy ; metabolism ; Dosage Forms ; Drug Evaluation, Preclinical ; Drugs, Chinese Herbal ; adverse effects ; chemistry ; pharmacokinetics ; Humans ; Magnolia ; chemistry ; Male ; Rats ; Rats, Wistar ; Substance P ; metabolism ; Vasoactive Intestinal Peptide ; metabolism

Objective To explore the factors related to the prognosis and survival duration of primary liver cancer patients after hepatectomy.Methods The data of primary liver cancer patientswho were treated by surgical resection were analyzed retrospectively.Kaplain-Meier method was used to evaluate survival rates.Log-rank test and Cox regression analysis were used to screen out related clinical phathology factors.Results The median survival time was eighteen months.Univarivate analysis showed that liver function Child-Pugh classification,cirrhosis,tumor size,HBV infection,AFP,portal vein tumor thrombus significantly correlated with survival rates (P ＜ 0.05).Multivariate analysis showed that liver function Child-Pugh classification,tumor size,AFP and portal vein tumor thrombus were the independent prognostic factors of primary liver cancer (P ＜ 0.05).Conclusion Many factors are related to the prognosis of primary liver cancer after operation.Liver function Child-Pugh classification,tumor size,AFP and portal vein tumor thrombus affect prognostic independently.

Objective To analyze the relationship between plasma aldosterone level and gestational diabetes melli?tus. Methods All subjects were divided into three groups (n=20 in each group):gestational diabetes mellitus (GDM) group, pregnancy with normal glucose group,non-pregnant healthy women group. Pre-pregnant body mass index (BMI) was calcu?lated, and fasting blood glucose (FBG), blood lipid level, Fins, HOMA-IR index, RASS, PRA, ATIi and ALD were examined besides glucose intolerance test. Results The plasma ALD level was significantly higher in GDM group than that of NGT group and control group(pmol/L:1 130.15±135.45 vs 1 053.98±108.86 vs 544.31±127.97). The plasma ALD level was posi?tively correlated with FBG,1 h PBG ,2 hPBG,Fins , HOMA-IR and blood triglyceride (r=0.657, 0.757, 0.713, 0.429,P<0.05),but negatively correlated with HDL-C(r=-0.267,P=0.039). Stepwise regression analysis revealed that plasma aldo?sterone was the independent risk factor of GDM. Conclusion RASS is excessively activated in pregnancy, which contrib?utes to gestational diabetes mellitus.

Animals ; Breast Neoplasms ; metabolism ; Humans ; NF-kappa B ; metabolism ; Neoplasms ; metabolism ; Precursor T-Cell Lymphoblastic Leukemia-Lymphoma ; metabolism ; Proto-Oncogene Proteins c-bcl-2 ; metabolism ; Receptors, Notch ; metabolism ; physiology ; Signal Transduction

Apoptosis ; Cell Proliferation ; DNA Methylation ; Humans ; Intercellular Signaling Peptides and Proteins ; genetics ; metabolism ; Membrane Proteins ; genetics ; metabolism ; Neoplasm Invasiveness ; Neoplasm Metastasis ; Neoplasms ; metabolism ; pathology ; Neovascularization, Pathologic ; Nerve Tissue Proteins ; genetics ; metabolism ; Promoter Regions, Genetic ; Receptors, Immunologic ; genetics ; metabolism ; Signal Transduction