1.Protective Effect of Electroacupuncture at Neiguan (PC 6) on Neurotransmitters Catecholamines in Rats with Myocardial Hypertrophy
Guangwei ZHU ; Song WU ; Jia LI
Shanghai Journal of Acupuncture and Moxibustion 2016;35(7):881-885
Objective To discuss whether acupuncture at Neiguan (PC 6) inhibits myocardial hypertrophy via monoamine neurotransmitters.Method Sixty female Sprague-Dawley rats were randomized into a control group, a model group, a sham electroacupuncture (EA) group, and an EA group, 15 cases in each group. The rat models of myocardial hypertrophy were developed by subcutaneous injection of Isoprenaline hydrochloride (ISO) for 14 d. The EA group was intervened by EA at Neiguan (PC 6), and the sham EA group was by EA at a sham acupoint [5 mm away lateral to Neiguan (PC 6). The Electrocardiogram (ECG) of each group was recorded, electron microscope was used to observe the microstructure of the rats’ myocardial tissues, and high performance liquid chromatography (HPLC) was adopted to detect the contents of catecholamines in myocardial tissues.Result The heart rate, R-R interval, ST-elevation, and the contents of catecholamines in rats’ myocardial tissues (adrenaline, noradrenaline, and dopamine) in the model group and sham EA group were significantly different from that in the control group (P<0.05). The heart rate, R-R interval, ST-elevation, and the contents of catecholamines in rats’ myocardial tissues (adrenaline, noradrenaline, and dopamine) of the EA group were significantly different from that of the model group (P<0.05).Conclusion EA at Neiguan (PC 6) can improve myocardial hypertrophy, which is related to the regulation of the topical neurotransmitters; it has provided laboratory evidences for the relationship between Neiguan (PC 6) and heart, i.e. the relationship between acupoints and internal organs.
2.Examination of Digital EEG Instrument
Liwei SONG ; Jiange JIA ; Yongxue LI
Chinese Medical Equipment Journal 1993;0(06):-
The digital EEG instrument is the use of that the electrical activity of cerebral cortex are extracted and recorded by electrodes, which diagnose and treat the human brain and nervous system. The examination methods that is strong interoperability is introduced based on JJG 954 -2000 Digital Electroencephalogram Mapping & Brain Electric Activity Mapping and combined with practical work experience for years.
3.Simultaneous Determination of the Contents of Geniposide and Liquiritin in Xinshenning Tablets by HPLC
Jia SONG ; Shuqing DUAN ; Yunxia LI
China Pharmacy 2015;(27):3862-3863,3864
OBJECTIVE:To establish a method for simultaneous determination of the contents of geniposide and liquiritin in Xinshenning tablets. METHODS:HPLC was performed on the column of Inertsil ODS-3 with mobile phase of acetonitrile-water (gradient elution) at flow rate of 1.0 ml/min,the column temperature was 25 ℃,detection wavelength was 238 nm and the vol-ume was 10 μl. RESULTS:The mass concentration range was 16.3-407.9 μg/ml for geniposide(r=0.999 9) and 7.4-185.9 μg/ml for liquiritin(r=0.999 9);RSDs of precision,stability and reproducibility tests were no more than 1.63%;average recoveries were 99.05%(RSD=1.28%,n=9)and 98.00%(RSD=1.84%,n=9),respectively. CONCLUSIONS:The established method is accu-rate,sensitive and simple,and can be used for the contents determination of geniposide and liquiritin in Xinshenning tablets.
4.Study on the relationship between chest radiograph changes and clinical features
Chinese Journal of Infectious Diseases 2001;0(06):-
Objective To observe the radiological feature of chest X ray in SARS patients. Methods The chest films of 54 cases of SARS were reviewed in the study. The chest film features and site of lung involvement were studies in early stage, most severe stage and recovery stage. The correlation between the score of lung involvement and clinical course was also studied. Results 79.63% cases had abnormal chest films in early stage. Most of them (72.22%) were found in 3~7 days after onset. The lung involvement was most common in bilateral (88.89%) and most in middle and lower field (46.30%). Exudation and consolidation lesions showed to be dominant. The lesions progressed quickly and showed to be variable. There are 17 (31.48%) cases had second peak in X ray, the mean time of onset is 14.71 days. The chest X ray is not parallel with clinical syndrome. The absorption of lung infiltrates is correlation with glucocorticosteroids treatment in 20 patients, and the correlation is not found in 26 patients.Conclusions The chest X ray of SARS has some features and its changes can reflect the clinical situation.
6.Accuracy of stroke volume variation in monitoring changes in blood volume during laparoscopic surgery
Aihua ZHAO ; Weiai JIA ; Chao LI ; Huiqun JIA ; Zixian SONG ; Shuxian LI
Chinese Journal of Anesthesiology 2015;35(4):447-449
Objective To evaluate the accuracy of stroke volume variation (SVV) in monitoring the changes in blood volume during laparoscopic surgery.Methods Forty ASA physical status Ⅰ or Ⅱ patients of both sexes,aged 40-64 yr,with body mass index ranged from 20 to 25 kg/m2,scheduled for elective laparoscopic surgery under general anesthesia,were studied.After induction of general anesthesia,baseline registrations of variables were obtained.After establishing pneumoperitoneum,6% hydroxyethyl starch (HES 130/0.4) 500 ml was infused over 30 min.Before pneumoperitoneum (T1),at 5 min after pneumoperitoneum (T2),immediately before volume expansion (T3) and at 3 min after volume expansion (T4),cardiac output (CO),cardiac index (CI),SV,stroke volume index (SVI) and SVV were monitored and recorded.The changing rate of CI (△CI) was calculated.The criterion for effective volume expansion was △CI ≥ 15%.The ROC curve for SVV in determining the volume expansion responsiveness was plotted,and the diagnostic threshold was determined.The area under the curve and 95% confidence interval were calculated.Results SVV was significantly lower at T2 than at T1.CO,CI,SV and SVI were significantly higher,and SVV was lower at T4 than at T3.The results of ROC curve analysis showed that a 9.2% SVV threshold discriminated between responders and non-responders with a sensitivity of 61% and a specificity of 50%,and the area under the curve (95% confidence interval) was 0.567 (0.378-0.757).Conclusion SVV is not a suitable index in monitoring the changes in blood volume during laparoscopic surgery.
7.Impact Factors of Hand Hygiene among Healthcare Workers:Investigation and Analysis
Lihong SONG ; Huixue JIA ; Jianxia JIA ; Xiuli ZHAO ; Yanchun ZHAO ; Linping LI ; Liuyi LI
Chinese Journal of Nosocomiology 1994;0(01):-
OBJECTIVE To know the compliance and it′s impact factors of hand hygiene of healthcare workers.METHODS The wards and healthcare workers were sampled randomly.Survey the compliance,qualified rate and impact factors of hand hygiene of healthcare workers when they didn′t know to be observed by special training staffs.RESULTS The compliance and the qualified rate of hand hygiene was 30.2% bothly,and be affected by the time before or after operation,occupations and work post.The factors causing low qualified rate were anenough time and improper technique for washing hand,and drying hand wrong.CONCLUSIONS The hand hygiene for healthcare workers in hospitals should be strengthened.
8.Preliminary evaluation of color power doppler ultrasonography in diagnosis of sacroiliitis in patients with ankylosing spondylitis
jia-an, ZHU ; bing, HU ; nian-song, WANG ; xiao-guang, ZHANG ; sheng-li, KUANG ; jia, LI
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(09):-
Objective To preliminarily evaluate the value of color power doppler ultrasonography in the diagnosis of sacroiliitis in ankylosing spondylitis(AS). Methods Fifty-seven sacroiliac joints in 31 patients with active AS and 40 sacroiliac joints in 20 volunteers were detected by color power doppler ultrasonography.The color flow signs inside the sacroiliac joints were observed,and the resistance index(RI) was measured. Results In active AS,color flow signs were seen in 55 joints,and the mean RI value was 0.53?0.08 in 45 joints,while the other 10 color flow signs represented reversed phase in diastolic phase on pulse doppler ultrasonography.In the volunteers,color flow signs were seen in 16 joints,and the mean RI value was 0.97?0.01 in 6 joints,while the other 10 color flow signs represented reversed phase in diastolic phase on pulse doppler ultrasonography. Conclusion The abnormal flow signs at the sacroiliac joints can be detected by color power doppler ultrasonography.Low RI values provide diagnosis evidence for active AS.
9.The characteristic of regional cerebral glucose metabolism in Wilson's disease using 18F-FDG PET
Xiang-song, ZHANG ; Ming, SONG ; Jia-jun, ZHUANG ; Li, LI ; Tian-zi, JIANG ; Xun-hua, LI
Chinese Journal of Nuclear Medicine 2008;28(5):333-335
Objective The aim of this study was to investigate the characteristic of regional cerebral glucose metabolism in patients with Wilson's disease (WD) using 18F-fluorodeoxyglucose (FDG) PET.Methods Thirteen WD patients and 12 normal controls were studied by brain 18F-FDG PET, and the data were analyzed by visual analysis, semi-quantification and statistical parametric mapping (SPM). The radioactivity ratios of lenticular nuclei, caudate, thalamus and cerebellum to cerebral cortex and the ratio of lenticular nuclei to caudate were calculated, respectively. SPSS 11.0 software for statistics was also used to analyze the data. Results In WD patients, radioactivity of lenticular nuclei and candate was significantly decreased compared with controls. The radioactivity ratios of lenticular nuclei and caudate to cerebral cortex in WD patients were both significantly lower than that in normal controls (0.95±0.05 vs 1.26±0.05, t =15, P < 0.05 ; 1.02±0.06 vs 1.17±0.05, t = 8, P < 0.05), and the ratio of lenticular nuclei to caudate in WD patients was significantly higher than that in normal controls (0.93±0.06 vs 1.09±0.06, t =9, P< 0.05). Conclusion As compared with normal controls, patients with WD had significantly decreased glucose utilization in the basal ganglia, especially in the lenticullar nuclei.