?Retinoblastoma is a common eye malignant tumor which has high degree of malignancy.The traditional treatment methods are destructive, and the prognosis is poor. Hypoxia-inducible factor-1αhighly express in retinoblastoma and regulate the development and progression of retinoblastoma ( by influencing the proliferation and differentiation of tumor cells and involving in the generation of vascular mimicry ) . This article reviewed the role of hypoxia inducible factor-1αin the development of retinoblastoma which will initiate a new avenue for the treatment of retinoblastoma.

BACKGROUND: The copper-superoxide dismutase (Cu-SOD) can not play an effective role in free radical-scavenging in the process of hypoxia or reaeration because of blood-brain barrier, whether the treating effect can be enhanced by chitosan nanoparticles is uncertain. OBJECTIVE: To observe the ability of chitosan nanoparticles loaded the Cu-SOD plasmid on free radical-scavenging in ischemia-reperfusion brain tissue. DESIGN: An observation experiment. MATERIALS: The Cu-SOD plasmid was provided by the Center Laboratory of Tangdu Hospital, Forth Military Medical University of Chinese PLA. A total of 36 rats were prepared ischemia-reperfusion models, and randomly divided into the control, physiological saline (PS) plus Cu-SOD plasmid and chitosan nanoparticles loaded the Cu-SOD plasmid groups. METHODS: The chitosan nanoparticles were prepared by cross-linking method. The Cu-SOD plasmid was absorbed to chitosan nanoparticles by electrostaficforces, mixed the 50.0 ?L nanoparticles with 10.0, 25.0, 50.0, 75.0, 100.0 ?L SOD plasmid respectively. The electrophoretic analysis was used to examine the combination condition of the chitosan nanoparticles with superoxide dismutase plasmid, and the embedding rate was calculated with ultraviolet spectrophotometer. Chitosan nanoparticles, SOD and Cu-SOD plasmid were infused into the rat after brain ischemia-reperfusion. Further more, to detect the content of malondialdehyde and superoxide dismutase in brain tissues. MAIN OUTCOME MEASURES: The combination condition of the chitosan nanoparticles with superoxide dismutase plasmid, and the activity of malondialdehyde (MDA) and SOD were measured. RESULTS: Chitosan nanoparticles and superoxide dismutase in volume ratio of 50: 75 could harvest the largest embedding rate with 100%, which had good protective effect on DNA. The contents of MDA in brain tissues of the Chitosan nanoparticles loaded the Cu-SOD plasmid group were obvious lower than that of the control (P

OBJECTIVE:To compare economy among sodium valproate (VPA),topiramate (TPM) and dipheninum (DPH) in the treatment of epilepsy (EP). METHODS:Retrieved from ProQuest,PubMed,Springer,CJFD,VIP,Wanfang database,re-searches about economy among VPA,TPM and DPH in the treatment of EP were collected. TreeAge Pro 2011.1.0.12.1 software was used to establish Markov model,and cost-effectiveness ratios (CER) of them were calculated to evaluate their economy. RE-SULTS:CER of DPH,VPA,TPM were 29.99,2 664.52 and 6 657.25,in ascending order of DPH

Objective:To study the antibacterial properties of pure titanium treated with benzalkonium chloride solution.Meth-ods:10 mm ×10 mm ×1 mm titanium specimens were processed by the benzalkonium chloride solution at 1%,0.5% and 0.1%respectively followed by treatment in the cultured bacterial suspension,and then the antibacterial properties of the titanium plates were examined.Additionally,the thermal cycling test was carried out for the 1% benzalkonium chloride-treated titanium plates, and subsequently put the plates into cultured bacterial suspension,the duration of antibacterial properties was observed.Results:0.5% and 1% benzalkonium chloride solution-treated titanium plates significantly inhibited the growth of candida albicans(P <0. 05),1% solution was more effective than 0.5% solution.After 1 000 and 2 500 thermal cycling,the pure titanium still retained the antibacterial ability,but the plates treated by 5 000 cycling showed no antibacterial effect.Conclusion:A certain concentration of benzalkonium chloride can make the pure titanium obtain antibacterial properties.The treated plates may maintain the antibacte-rial properties for a minimum of 3 months.

Objective In order to get reference data for diagnosis of clinical genetic disease through analyzing chromosome ab‐normality types and rates in 885 patients who ask for cytogenetics consultation in recent years .Methods 324 newborns who asked for cytogenetics consultation because of high risk factors in down′s screening during pregnancy or found abnormality in physical ex‐amination after birth and 561 patients with history of spontaneous abortion ,infertility or fetal death ,growth or mental retardation , sexual abnormality were examined for karyotype analysis .Results 116 cases of chromosome abnormal karyotypes were detected , count for 13 .11% ,among which ,40 cases(34 .48% ) are chromosomal aberration .Chromosome abnormality types and rates are dif‐ferent in patients with different type of diseases .Conclusion Abnormalities in chromosome numbers and sex chromosome abnor‐mality account for the main causes of growth and mental retardation ,abnormalities of sexual differentiation and development ;but for couples suffered from spontaneous abortion ,polymorphism and structure abnormalities takes up the highest portion .

BACKGROUND:Artificial teeth computer aided design and computer aided manufacture (CAD/CAM) is a high technology involving various subjects.Many CAD/CAM systems have designed.However,they have some disavantages and a high cost.Therefore,only the system with proprietary intellectual property can solve this problem.OBJECTIVE:To optimize the connector of the CAD/CAM fixed bridge.METHODS:Three-dimensional finite element models of the connector were established which was used to design different styles of connectors.Stress under different vertical loading conditions was compared.RESULTS AND CONCLUSION:The connectors with different appearances displayed varying stress.Stress on the connector with arc transition was less than connector without arc transition.Connector was supposed to design to style with arc transition.

Objective To analyze the skeletal structure features of temporomandibular joint (TMJ) by using cone beam computed tomography (CBCT) in patients with sleep bruxism. Methods CBCT scanning was performed in the intercuspal position in 30 patients with sleep bruxism (sleep bruxism group) and 30 health adults (normal group). The reconstruction of TMJ images were analysed by Invivo5 software. Measurements of horizontal section include antero-posterior diameter, medial-lateral diameter and axial angel. Measurements of sagittal section include acetabulum depth, aricular eminence inclination, anterior space, superior space and posterior space. Measurements of coronal section include force angle. Results There were no significant differences in both sides of TMJ between sleep bruxism group and normal group(P>0.05). Compared with control group, there were significant differences in antero-posterior diameter, medial-lateral diameter, acetabulum depth, anterior space and force angle in sleep bruxism group (P<0.05). There were decreased antero posterior diameter, me?dial-lateral diameter and increased acetabulum depth, anterior space and force angle in sleep bruxism group than those of control group. Conclusion The bilateral bone stuctures of TMJ are basically symmetry in patients with sleep bruxism. There is no significant change in condylar position in acetabulum , but there is a trend of absorbtion of anterior space and de?crease of condyle in patients with sleep bruxism.

Objective To explore the effects of Sanban manipulation of spine on ankylosing spondylitis (AS) at the early-middle stage.Methods 60 AS patients at the early-middle stage were divided into drug group (n=30) and manipulation group (n=30). The drug group wastreated with sulfasalazine and the sustained-release pills of Zhengqingfengtongning, while the manipulation group were treated with Sanbanmanipulation of spine additionally. Self-developed questionnaire were used to follow up before and 1, 3 and 6 months after treatment. ResultsThe Schober index and the finger-floor distance improved more in the manipulation group than in the drug group (P<0.05) 3 months aftertreatment. All the index improved more in the manipulation group than in the drug group (P<0.05) 6 months after treatment. ConclusionSanban manipulation of spine can improve the thoracolumbar activities in the AS patients at the early-middle stage.

Objective To establish the quality standard of Qingshen Granules. Methods Thin-layer chrmatography was used to identify Rheum officinale Baill., Hedyotis Diffusa Wild. and Coptis chinensis Franch.. The contents of rhein, emodin and chrysophanol were determined by HPLC. The HPLC consisted of Kromasil C18 (4.6 mm×250 mm, 5 μm), methanol-0.1% phosphonic acid (70∶30) as mobile phase, flow rate of 1.0 mL/min, detection wavelength at 245 nm, and the column temperature was 30 ℃. Results The results of TLC showed that relevant spots were clear without interference against the negative sample. The calibration curves for rhein, emodin and chrysophanol were found to be linear within the range of 0.003 3-0.42 μg (r =0.999 9), 0.008 0-0.51 μg (r=1.000 0), 0.009 9-0.32 μg (r=1.000 0), respectively. The average recoveries were 98.84%, 99.04% and 100.35% with RSD of 1.30% (n=6), 1.34% (n=6) and 1.89% (n=6), respectively. Conclusion The methods are accurate and quick in qualitative identification and quantitative assay, and can be used for the quality control of Qingshen Granules.

Objective To research the structure,imaging mechanism and quality of dry view laser imaging film. Methods We extracted 60 pieces of dry view laser imaging film,wet view laser imaging film and ordinary film respectively. Each tape of films was divided into 3 groups in 100 pieces each. They were observed in the same temperature and humidity. Results Group A was observed after 300 minutes,the dry view laser films image of 10 pieces without package,4 pieces packed with plastic and 1 piece packed with paper changed. After 120 minutes,group B was observed; the dry view laser imaging film in different packing had the different changes. To the contrast,the wet laser imaging film's images didn't change in the same humidity and temperature after 420 minutes test. Conclusion The imaging of dry laser imaging film with different temperature,humidity and package will easily affect the image quality.