OBJECTIVE To discuss the constitution and drug sensitivity of pathogens that cause pulmonary infection in NICU. METHODS Sputa were collected from patients hospitalized in NICU from Mar 2006 to Mar 2007 and analyzed for distribution and drug sensitivity of pathogenic bacteria. RESULTS All 555 strains of pathogenic bacteria were isolated. The most common pathogens were Pseudomonas aeruginosa(183 strains,33.0%),Staphylococcus aureus(114 strains,20.5%) and Klebsiella pneumoniae(56 strains,10.1%). Nearly most pathogenic bacteria were multi-resistant to commonly used antibiotics. Meticillin resistant strains of S. aureus accounted for 97.4%. The percentange of fungi strains was increasing in NICU. The main isolated strains were Candida tropicalis(5.9%),C. albicans(3.8%)and C. glabrata(3.6%). And all of the fungi were sensitive to amphotericin B. fluconazole. and ketoconazole. CONCLUSIONS Pulmonary infections in NICU are mainly caused by Gram-negative bacteria with high rate of drug resistance. It can be of great importance to make drug sensitivity tests at regular intervals to guide the use of antibiotics.

Two hundred and thirty two patients with essential hypertension were divided into intervention and control groups (116 in each) ,by the end of study period (from January 2005 to January 2006) 106 patients in intervention group (91.4%) and 102 in control group (87.9%) completed observation.The main findings were as follows: the blood pressure control rate in intervention group was higher than that in controls (X2=23.92,P <0.01) ;the family adaptability(X2 =31.12,P <0.01),family caring(X2=37.90 ,P<0.01),family communication (X2= 17.91,P<0.01) and family function(X2 = 8.26,P<0.01)in intervention group were better improved than those in control group.The results suggest that family can be effective resources for improvement of patients with essential hypertension.

Acupuncture Therapy ; Female ; Humans ; Polycystic Ovary Syndrome ; etiology ; therapy

Female ; Humans ; Obesity ; complications ; Polycystic Ovary Syndrome ; complications ; therapy

Objective To detect the new views for pulmonary vascular by transesophageal echocardiography(TEE) during lung transplantation.Methods Seventeen cases with respiratory failure underwent lung transplantation.A series of scans were detected by TEE during preoperative anesthesia, preand pro-pulmonary vascular occlusion and pro-donor lung, and bronchus, pulmonary veins, pulmonary artery anastomoses.Left and right lung veins open and left and right pulmonary artery branch were key to observed.Pulmonary artery trunk and branch diameter, velocity of blood flow were measured.Results All left and right pulmonary artery,pulmonary vein images were given by TEE.TEE detected the normal blood flow velocity of pulmonary artery branches during pre-operation,average of 65 cm/s.After clipping the side of the pulmonary artery, the lateral pulmonary blood flow disappeared.TEE detected pulmonary veins,pulmonary artery anastomoses pro-operation.Except for 1 case of anastomotic pulmonary vein flow velocity increased,all the pulmonary vein and pulmonary artery branch anastomoses flow velocity increased slightly faster than those before transplantation (mean pulmonary artery branch of 160 cm/s, the average pulmonary vein 149 cm/s).Conclusions TEE can monitor diameter and flow velocity of pulmonary artery branch and pulmonary vein during lung transplantation,and can detect the rightventricular function.The views of left and right branch pulmonary artery and pulmonary vein branches play an important role during lung transplantation.

Female ; Humans ; Polycystic Ovary Syndrome ; therapy

BACKGROUND: Monoamine hypothesis has been demonstrated by researches. However, the correlation between the metabolite of plasma monoamine neurotransmitter and anti-depression treatment in patients with depression has less been reported.OBJECTIVE: To study the effect of different drugs on metabolite of plaama monoamine neurotransmitter, and the correlation between the metabolite of plasma monoamine neurotransmitter and anti-depression treatment in patients with depression.DESIGN: Case controlled study.SETTING: Neurological Department and Brain Institute of Nanjing Medical University.PARTICIPANTS: Forty patients with depression hospitalized in Nanjing Brain Hospital (depression group) were diagnosed with the second revised edition of China classification of mental diseases(CCMD-2) and the tenth edition of International classification of diseases. And the total score of Hanmilton rating scale for depression(HAMD) was more than 17. Healthy voluntary blood donators in the control group were from Nanjing Municipal Central Blood Station( n = 20).INTERVENTIONS: Antidepressant was used in the depression for 4 weeks: fluoxetine 20 mg per day; 5-serotonin selective reuptake inhibitor (SSRI) paroxetine 20 mg per day; venlafaxime 50- 100 mg per day;5-serotonin and morepinephrine selective reuptake inhibitor(SNRI) fluvoxamine 50-100 mg per day. High performance liquid chromatograpy(HPLC)was used to measure the level of metabolite of plasma monoamine neurotransmitter in patients with depression before and 42 week after treatment, and the HAMD was used to evaluate clinical effect of the patients.MAIN OUTCOME MEASURES: The levels of metabolites of plasma monoamine neurotransmitters in patients with depression: 5-hydroxyindoleace tic acid(5-HIAA), 3-methoxy-4-hydroxyphenylglycol(MHPG) and homovani llic acid(HVA) were measured before and 4th week after treatment.RESULTS: The levels of 5-HIAA, MHPG and HVA of the metabolites of plasma monoamine neurotransmitters in patients with depression before treatment [ (20.3±14.6), (124.8±103.6), (54.7±32.1) μg/L] were all lower than those in the normal control group[ (39.5±28.4), (334.5 ±107.3), (88.5±37.2) μg/L], with statistically significant differences (P＜0.05). After SSRI treatment, the 5-HIAA content[ (37.1±21.9)μg/L]was significantly increased as compared with that before treatment, whose difference indicated significant meaning ( P＜0.05), but the differences in MHPG and HVA had no significant meaning as compared with those before treatment(P＞0.05) . After SNRI treatment, 5-HIAA and MHPG contents [(35.4±25.2 ), (291.2±120.4) μg/L] both were significantly increased, which indicated significant difference as compared with those before treatment( P＜0.05); but HVA level had no significant changes.CONCLUSION:'The peripheral neurotransmitter metabolites in plasma can reflect their states in brain. The change of neurotransmitter metabolite in plasma can be regarded as an important reference index for the evaluation of depression.

Simultaneous distillation-extractor was used for extracting the volatile substances in Phellodendron chinense Schneid.41 Volatile components were identified by gas chromatography/mass spectrometry (GC/MS) method. Of them the main components are ketones(16.38%),aldehydes(13.94%),alcohols(8.27%),phenols(50.38%),esters(3.02%),acids(2.18%).The content of the 41 identified compounds makes up 97.50% of the total volatile substances detected

Objective To investigate the mRNA expression level of the hepatocyte growth factor receptor, c-met, and heterogeneous nuclear ribonucleoprotein, HnRNPA2/B1, in non-small cell lung cancer (NSCLC) patients and their relationships with invasion and metastasis of NSCLC. Methods The mRNA expressions of the c-met and HnRNP A2/B1 in postoperative samples of 46 patients with NSCLC and tissue samples of 30 patients with lung innocence disease as normal controls were detected by RT-PCR, and the efficacies of each marker and combining both of markers in the diagnosis of NSCLC were analyzed by Chi square test. Results The positive rates and relative expression quantity of c-met [65.2 % and (0.903±0.04)]and HnRNP A2/B1 [60.9 % and (0.162±0.04)] in NSCLC were significantly higher than those in control group [26.7 %, (0.205±0.06) and 20.0 %, (0.096±0.02), respectively] (P ＜0.05), and the positive rate of combining both was higher than that of single marker for NSCLC diagnosis(P ＜0.05) . The overexpression of two markers was also significantly correlated with the N stage and clinical stage, but not with age, gender and pathologic types. Conclusion The high expression of c-met and HnRNP A2/B1 in NSCLC may be of significance for the clinical stage. Combining test of two markers provides more sensitivity for NSCLC diagnosis. The expression change of two markers may involve in the carcinogenesis and development of NSCLC.

OBJECTIVE:To establish the method for simultaneous determination of 6 components in Lonicera japonica,and to study the content changes of them before and after before and after carbonized. METHODS:UPLC method was adopted. The deter-mination was performed on Agilent Eclipse Plus C18 RRHD column with mobile phase consisting of 0.1% phosphoric acid solu-tion-acetonitrile(gradient elution)at the flow rate of 0.2 mL /min. The detection wavelength was set at 350 nm,and column tem-perature was 25 ℃. The sample size was 1 μL. RESULTS:The linear ranges of chlorogenic acid,rutin,galuteolin,isochlorogenic acid A,isochlorogenic acid B and isochlorogenic acid C were 21.2-424 μg(r=0.9993),1.17-23 μg(r=0.9995),2.18-43 μg(r=0.9998),5.10-102 μg(r=0.9993),2.60-52 μg(r=0.9991),4.95-99 μg(r=0.9998),respectively. RSDs of precision,stability and repeatability tests were all lower than 2.0%. Recoveries were 97.11%-99.76%(RSD=1.20%,n=6),95.20%-99.90%(RSD=2.20%,n=6),95.71%-100.30%(RSD=2.20%,n=6),95.00%-96.98%(RSD=0.88%,n=6),96.47%-103.00%(RSD=2.40%, n=6),95.78%-103.80%(RSD=3.20%,n=6). Compared with before processing,the contents of rutin,isochlorogenic acid B and isochlorogenic acid C in L. japonica were increased along with processing,the contents of chlorogenic acid and isochlorogenic acid A were decreased significantly,while the content of galuteolin had no significant change. CONCLUSIONS:The method is sim-ple,precise,stable and repeatable,and can be used for simultaneous determination of 6 components in L. japonica. Those chemi-cal components have certain changes before and after carbonized.