Objective To explore the clinical value of the immune rate nephelometry (IRN)in detecting the rate of serum ? and ?,and diagnosing multiple myeloma.Methods Twenty-five cases of multiple myeloma (MM),28 cases of M protein sickness and 120 cases of healthy people were detected for the rate of serum ?/? by the Immage Protein Machine the results were analyzed.Results 0.8% of healthy people was positive, the positive rate of ? and ? type M protein sickness were 23.1% and 26.7% respectively, the positive rate of ? and ? type multiple myeloma was 92.3% and 100% respectively. Conclusion The method of IRN has high sensitivity and stability in detecting the light chain of serum ? and ? on/with the Immage Protein Machine.The accuracy value of prognosing MM with the rate of serum ? and ? is better.

Objective To compare the values of helical CT Ray sum projection(RSP)with shaded surface display (SSD)in diagnosing pelvic lesion.Methods Ray sum images in 18 cases of ankylosing spondylitis,5 cases of bone tumor,15 cases of pelvic fracture were analyzed and compared with shaded surface display(SSD),plain films and CT scans.Results The positive rate of 18 cases ankylosing spondylitis was 93.5% for RSP,38.7% for SSD and 83.9% for plain films.In 12 cases of 1～2 stages articular sacroiliaca arthritis,RSP can detect sacroiliac joints sclerosis and vesicula osteonecrosis as well as the axial view,it had higher positive rate than plain film or SSD(?

ObjectiveTo detect the ultrastructure of neural stem cells (NSCs) cultured in vitro .MethodsNSCs separated from the cortex of 17—19 days Wistar rat fetus were cultured and induced to differentiate in vitro. Electron microscopes were used to visualize the ultrastructure of these cells before and after differentiation.ResultsNSCs had the similar cellular size, morphology and intracellular structures pre-differentiation. Cells were able to proliferate via mitosis. The nucleus/cytoplasm ratio was very high. The nucleus was poly-morphological. Cells had very little cytoplasm and no mature organelles. After differentiation, several processes protruded out from cellular surface. Cells became flat shape, the volume of cytoplasm increased dramatically and various kinds of mature organelles appeared in the cytoplasm. Cells differentiated into two kinds of cells,neural cells and glial cells,with quite different morphology and intracellular structure. ConclusionNSC is one kind of original cells which can be induced to differentiate into mature neural cells and glial cells.

AIM: To study the causes of pyogenic granuloma after hydroxyapatite(HA) orbital implants.METHODS: HA orbital implants (250 cases) in our hospital (68 pegged implants) were reviewed.All patients were followed up from 18 months to 10 years. Implants were removed after medical therapy which was proved to be ineffective.RESULTS: Ten of 250 cases of HA orbital implants developed pyogenic granuloma. Pyogenic granuloma occurred in 1 unpegged implants patient and 9 patients after pegging and drilling of HA implantation over 4～7 years. The pyogenic granulomas were not controlled by medical therapy effectively. Implants were removed in 9 cases except 1 case denied removing and continued medical therapy.CONCLUSION: Pyogenic granuloma was serious complication that occurred after HA orbital implants. Partial vascularization, implant exposure, xenogenic sclera implant, pegging and drilling of HA implantation are risk factors that affect the development of pyogenic granuloma.Pyogenic granuloma hasn't relation with implanted peg material. Pyogenic granuloma denotes the potential implant infection, and all implants should be removed finally.

OBJECTIVETo study operative effects for the treatment of multiple ligament injuries of knee joints.

METHODSFrom 2008 to 2013, 26 patients (17 males and 9 females) with multiple ligament injuries of knee joints were treated surgically. The average age was 40.7 years old, ranging from 29 to 55 years old. All the patients were treated with arthroscopic reconstruction of cruiate ligament with autogenous or allogeneic hamstrings and tendon, and at the same time received repair of medial collateral ligament and lateral collateral ligament, as well as the treatment of exterior and interior complex injuries. Nine patients received second stage operation after the initial operation for mistake or missed diagnosis, and other patients were treated at the first stage. The Lysholm scoring system was used to evaluate function and stability of knee joints before and after operation.

RESULTSAll the patients were followed up for an average duration of 1.6 years (ranged, 0.8 to 3.2 years). The mean awaiting time for operation was 1.2 months. The Lysholm score was improved from preoperative 42.5 +/- 4.5 (ranged, 33 to 48) to the latest follow-up 78.1 +/- 3.9 (ranged, 57 to 95). The function of knee joint was improved obviously in the arthroscopic reconstruction patients, with joint range of motion exceeding 900 and with Varus & Valgus tests near to normal. All the patients had negative findings in the Lachman test at 70 degrees of flexion.

CONCLUSIONArthroscopic reconstruction should be the first choice in treating multiple ligament injuries of knee joints. If the anterior and posterior cruciate ligament injuries can't be treated simultaneously, the posterior cruciate ligament injuries should be treated preferentially at the first stage and the anterior cruciate ligament injuries should be treated at the second stage. The diagnosis of posterior cruciate ligament is easy to be missed.

Adult ; Arthroscopy ; Female ; Humans ; Knee Joint ; physiopathology ; surgery ; Ligaments ; physiopathology ; surgery ; Male ; Middle Aged ; Multiple Trauma ; physiopathology ; surgery ; Range of Motion, Articular ; Treatment Outcome

OBJECTIVETo explore surgical therapeutic strategies and clinical effects for floating shoulder injury.

METHODSFrom March 2010 to December 2013, 12 patients with floating shoulder injury were treated by open reduction and plate screw fixation,including 10 males and 2 females aged from 22 to 45 years old with an average of 31.7 years old. Preoperative X-ray and CT showed 11 cases were ipsilateral clavicle fracture with scapular neck fractures, 1 case was scapular neck fracture with ipsilateral acromioclavicular dislocation. Postoperative complications were observed and Herscvici functional scoring were used to evaluate clinical effects.

RESULTSTwelve patients were followed up from 8 to 26 months (averaged 15 months). All patients obtained bone union, and no inflammation, loosening and breakage of plate and screw were found. According to Herscvici scoring, 9 cases got excellent results, 2 good and 1 moderate.

CONCLUSIONReconstruction of anatomical structure and stability of floating shoulder joint injury could shorten time of shoulder joint brake and fixation, then got excellent clinical outcomes.

Adult ; Clavicle ; injuries ; Female ; Fractures, Bone ; surgery ; Humans ; Male ; Middle Aged ; Reconstructive Surgical Procedures ; Scapula ; injuries

Ethnic medicine industry is facing many problems such as narrow market, exhaustion of resource, decline of ethnic medicine and no qualified successors. Sustainable development theory was utilized to analyse the elements and problems of ethnic medicine industry, and the counter measures were put forward to get rid of the predicament and to realize the sustainable development which depends on the ethnic medicine resources, national medicine, industrial policy, personnel training and modern technology. The development issues of ethnic medicine industry can be solved by the coordination of enterprise, government and public. Finally the ethnic medicine can provide better services for society.
China ; ethnology ; Conservation of Natural Resources ; economics ; Drug Industry ; economics ; manpower ; Drugs, Chinese Herbal ; economics ; Humans ; Medicine, Chinese Traditional ; economics

Objective To investigate the prophylactic effect of thymosin α_1 on acute exacerbation of chronic obstructive pulmonary disease(AECOPD)in elderly patients.Methods One hundred and twenty COPD patients were randomized into two groups(n=60 for each).Patients in the treatment group received thymosin α_1 1.6 mg subcutaneously,2/week for 8 weeks;while patients in control group received normal saline instead.Serum CD3,CD4,CD8,IrA,IgG and IgM levers were measured,and the pulmonary function were examined before and 2.12 months after treatment.Results In treatment group the recurrence interval of AECOPD Was extended:the attack frequency and persistence time of AECOPD were significantly lower in cutuparison with those of the control group(P＜0.05).CD4 and CD4/CD8 levels were significanfly increased(P＜0.05)after treatment of thymosincd.Conclusion Thymosin α_1 Can protect acute exacerbation of chronic obstructive pulmonary disease in elderly patients.

Objective:To prepare for mAb of progesterone receptor. It would provide support for the immunohistochemistry behind. Methods:Target gene connected together with a carrier by seamless cloning method. The target protein that expression by inducing was collected. And with cell fusion method , the monoclonal antibodies were preparation. Then the mAb were detected by IHC. Results: The mAb ( clone 7C7 ) was detected and it found positive for the breast, uterine fibroid tissue, showed negative in colorectal cancer tissue, smooth muscle tissue, the goal of the claim were achieve. Conclusion: Finally, we found the method that prepare for mAb was far beyond our imagination. The result of IHC on different samples about mAb(7C7)obtained compliance with an-ticipation. Study on the difference between the PR-A and PR-B had significance.

Objective To observe the effect of Xiaoyu Huatan Decoction (XHD) on hepatic apoptosis and apoptosis- related gene Bcl-2,Bax expression in nonalcoholic fatty liver disease(NAFLD)rats,and to explore its therapeutic ef- fect for NAFLD.Methods The rat model of NAFLD was induced by feeding high fat forage.Dongbao Gantai was used as the positive control drug.Flow cytometry(FCM)was used to detect the hepatic apoptosis and expression of Bcl-2 and Bax,and optical microscope was used to observe the degree of hepatic steatosis.Results Moderate or serious steatosis of liver cells was tound under optical microscope,and there existed a few hepatic necrosis.The apoptotic rate and FI expres- sion of Bax in the model group were significantly increased (P