Asthma ; immunology ; Humans ; Rhinitis ; immunology

Allergens ; immunology ; Child ; Desensitization, Immunologic ; Humans ; Rhinitis, Allergic, Perennial ; immunology ; therapy ; Treatment Outcome

Desensitization, Immunologic ; Humans ; Rhinitis, Allergic, Perennial ; therapy ; Rhinitis, Allergic, Seasonal ; therapy

China ; Chronic Disease ; Humans ; Phenotype ; Rhinitis ; genetics ; immunology ; Sinusitis ; genetics ; immunology

AIM: Pancreatic and duodenal homeobox gene 1(pdx-1) is a crucial transcription factor in pancreatic islet development and differentiation. This study was conducted to evaluate whether pdx-1 delivered by adeno-associated virus (AAV) could induce liver cells to differentiate into insulin-producing cells in diabetic rats and thus provide more information for cell replacement therapy for diabetes. METHODS: Recombinant AAV vector was employed to deliver pdx-1to STZ-induced diabetic rats via portal vein (4×1011). Blood glucose and body weight were monitored. Gene expression of pdx-1 and insulin were determined by RT-PCR and immunocytotochemistry (ICC) at the 6th week after the injection. RESULTS: AAV-pdx-1 group showed obvious gene expression of pdx-1 and insulin by RT-PCR analysis and the presence of more insulin-positive cells by ICC. Hyperglycemia was partially ameliorated and body weight was also increased in AAV-pdx-1 treated diabetic rats, though still significantly different from those in the non-diabetic group. CONCLUSION: The data indicate that AAV-pdx-1 can induce more rat liver cells into insulin-producing cells in vivo, thereby ameliorate hyperglycemia. Further experiments are needed to explore which subpopulation of liver cells responds to this development shift and the mechanism of this development shift induced by pdx-1 in order to improve the differentiation efficiency.

Objective　To study the effect of rehabilitation training on the expression of Fos in the central nervous system(CNS) of rats with cerebral infarction. Methods　60 SD rats were made as cerebral infarction models and divided into 2 groups at random 24 hours later: rehabilitation group was given balancing, grasping, rotating and walking and other trainings everyday, while immobiligation group was fixed in cages. Immunohistochemistry was used to detect the Fos expression 1 d, 7 d, 14 d, 21 d and 28 d after infarction respectively. Results　Fos-positive immune reaction appeared in cortex,thalamus, hypothalamus, reticular form and posterior gray horn of the spinal cord of both groups　.Rehabilitation group responded stronger than the immobilization group significantly (P<0.01).Conclusion　Rehabilitation training can activate cortical neurons around the infarted focus and in its opposite side of the CNS, and thus promote the recovery of rats' motor functions.

Objective The study aimed to investigate the effect of lipoxin A4 (LXA4) on lipopolysaccharide (LPS)?induced oxidant stress in human renal tubular epithelial cells (HK2 cells) and possible underlying mecha?nisms. MethodsHK2 cells were divided into three groups: Control ,LPS and LPS+LXA4 groups. After cells were treated with indicated conditions,morphological changes were observed. The expressions of Nrf2 were detected by immunofluorescence and cells were collected for RT?PCR experiments.Results HK2 cells seemed disrupted and necrotic with the administration of LPS. However ,LXA4 could prevent cells from injury induced by LPS. LPS decreased Nrf2 expression and promoted it to translocate to cytoplasm ,while LXA4 could increase its expression and promote it to translocate to nucleus. Moreover ,LPS could decrease Nrf2 and its downstream molecule mRNA expressions,but LXA4 could reverse this effect. Conclusion Our results demonstrated that LXA4 effectively inhibit?ed HK2 cell oxidant stress via Nrf2 pathway.

Female ; Humans ; Infant ; Male ; Ultrasonography ; Urachus ; abnormalities ; diagnostic imaging

Objective To evaluate the effect of methylene blue and visible light by FQ-PCR assay on the inactivation of plasma hepatitis B virus,and to establish a direct and impersonal basis for the surveillance of cural inactivation.Methods Four samples of plasma of HBV-DNA positive were added with methylene blue. Then the plasma was processed with visible light radiation(30 000 Lux) for 0, 5, 10, 15,30 min respectively. Moreover,the concentration of HBV-DNA samples was quantitatively detected by the method of FQ-PCR and compared with untreated samples to evaluate the effect of HBV inactivation and its relationship with the time of radiation.Results The original concentration of HBV-DNA was 5.6?107, 3.2?106, 1.8?106,3.5?105 copies/ml respectively. After being added with methyene blue, the concentration of HBV-DNA was significantly decreased(P

3 months) showed that 47. 2% of 36 patients had persistent relief without pain recurrence. Besides pain relief, the interventional endoscopy resulted in weight gain and improvement in quality of life in 63. 9% of 36 patients. Furthermore, the incidences of hyperamylasemia and bleeding which recovered after medication only within 3 days were 13. 9% and 5. 6% , respectively. Stent occlusion and detachment occurred respectively in 12. 5% and 4. 2% of 24 patients undergoing stent placement. Conclusion Endoscopic therapy for patients with pancreatic duct stricture appears to be safe and effective.