OBJECTIVE:To establish an HPLC method for simultaneous determination of Naringin,Hesperidin and Neohesperidin in the effective fraction of flavone in Fructus Aurantii Immaturus.METHODS:Hypersil C18 column(250 mm?4.6 mm,5 ?m) was used with the mobile phase consisted of acetonitrile -0.1% phosphoric acid solution(20∶80) at a flow rate of 1.0 mL?min-1,and the detection wavelength was set at 283 nm and the column temperature was maintained at 30 ℃.RESULTS:The calibration curves of Naringin,Hesperidin and Neohesperidin were in good linearity over the ranges of 0.44～2.20 ?g(r=0.999 5),0.025 6～0.128 0 ?g(r=0.999 3),0.54 ～ 2.70 ?g(r=0.999 9),respectively.And the average recoveries for the three constituents were 99.41%,100.33% and 99.69%,respectively with RSD at 1.14%,1.47%,and 1.16%,respectively.CONCLUSION:The method is simple,accurate and reliable,and can be used for the quality control of Fructus Aurantii Immaturus.

Objective To study the new anticoagulants, felling shaaban joint care used in joint replacement for prevention of deep vein thrombosis.MethodsThe Second People's Hospital of Chengdu orthopaedics in March 2016 to March 2017 were hospitalized patients with knee joint replacement and total hip replacement of the 158 patients, according to the stochastic indicator method were randomly divided into observation group and control group, 79 cases in each group, the observation group received new anticoagulants, cutting class joint care, the control group accepted low molecular weight heparin calcium in combination with care.Observe two groups of treatment of deep vein thrombosis prevention effect and adverse reactions.ResultsCompared with control group, the observation group with lower incidence of DVT and difference comparing the two groups have statistical significance (P<0.05).Observation group compared with control group, PT, APTT, preoperative and postoperative 2 day, five days did not have obvious difference, no statistical significance.Observation group compared with control group, subcutaneous ecchymosis, incision hematoma bleeding had no obvious difference).ConclusionThe laval shaaban joint care to prevent deep venous thrombosis after artificial joint replacement effect of low molecular weight heparin calcium is better, and coagulation function effect is small.

Disease Progression ; Humans ; Infant, Newborn ; Infant, Premature ; Infant, Premature, Diseases ; pathology ; Leukomalacia, Periventricular ; pathology

Glycogen synthase kinase-3βis a multifunctional Ser/Thr kinase , and its activity has been associated with many cell processes .The mitochondrial permeability transition pore is primed by ischemia to open upon reperfusion , leading to reperfusion induced cell necrosis .Phosphorylated GSK-3βpresumably inhibits mPTP opening by multiple mechanisms , including preservation of hexokinaseⅡin mPTP complex , prevention of interaction of cyclophilin-D with adenine nucleotide translocase , inhibition of P53 activation and attenuation of ATP hydrolysis during ischemia .

Objective To study action mechanism of rhamnocitrin in Oxytropis Falcata on enhance the immune function. Methods Kunming mice were randomly divided into blank control group, model group, positive control group, traditional Chinese medicine high, medium, and low dose groups. All treatment groups received gavage with relevant medicine for 10 days. From the 8th day, blank control group received intraperitoneal injection with normal saline, while other groups received intraperitoneal injection with cyclophosphamide for 3 days. Through macrophages carbon clearance test, macrophage phagocytosis of chicken erythrocytes test, and immunosuppressive mice organ index detection, effects of rhamnocitrin in Oxytropis Falcata on nonspecific immune function of immunosuppressive mice were observed. Results Compared with model group and blank control group, traditional Chinese medicine high, medium, and low dose groups could improve the phagocytic activity of immunosuppressive mice, clearance index, and phagocytic coefficient (P<0.05), and increase the organ index of the thymus and spleen. Compared with the positive control group, the efficacy of traditional Chinese medicine high, medium, and low dose groups was low. Conclusion Rhamnocitrin in Oxytropis Falcata can enhance the immune function, improve the formation of spleen cells of mice serum hemolysin of chicken red blood cell immune.

BACKGROUND: Taohong Siwu Tang is made up of Taoren, Honghua,Danggui, Chishao and Chuangxiong. Paeoniflorin is one of the major effective components in Radix Paeoniae Rubra Extracts and Taohong Siwu Tang, and it can promote blood circulation and remove blood stasis and has the effect of anti-acute myocardial ischemia.OBJECTIVE: To compare the serum concentration of paeoniflorin in mice after intragastric administration (i.g.) of Radix Paeoniae Rubra extracts and Taohong Siwu Tang.DESIGE: Rndomized controlled observation.SETTING: Department of Pharmacy and Department of Basic Medicine,Hebei North University.MATERIALS: The trial was performed in the first laboratory of Department of Pharmacy, Hebei North University during February to June 2005.Eighty involved Kunming mice of either gender and of clean grade, with body mass of 18 to 22 g, were purchased from Experimental Animal Center, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences. Taoren, Honghua, Danggui, Chishao, Shengdi and Chuanxiong 6 Chinese herbs were all purchased from Zhangjiakou Institute of Traditional Chinese Medicine.METHODS: ①Blank serum was isolated from eight mice which were fasted for 12 hours. Control sample solution of paeoniflorin was added into blank serum. Acetonitrile was used to deposit protein, the solution was centrifugated and isolated ,and 20μL supernatant was taken to obtain regression equation. ② Seventy-two Kunming mice were randomized into Radix Paeoniae Rubra extracts group and Taohong Siwu Tang group, with 36 mice in each. After being fasted for 12 hours, the mice in each group were intragastric administration of Radix Paeoniae Rubra extracts and Taohong Siwu Tang, respectively and the concentration of paeoniflorin was 300 mg/kg.1 mL blood was taken respectively at 15, 30, 60, 90, 120 and 180 minutes after intragastric administration and prepared into serum sample (6 mice at each time point in each group). The time to peak and difference in peak concentration of serum paeoniflorin were compared between intragastric administration of Radix Paeoniae Rubra extracts and Taohong Siwu Tang.The concentration of paeoniflorin was measured with high-performance liquid chromatography (HPLC). Chromatographic condition: chromatographic column YWG-C18 (250×4.6 mm,10 μm). The mobile phase was methanolKH2PO4 (0.05 mol/L) (38∶62) at a flow rate of 1.0 mL/min. The detective wavelength was set at 230 nm. The column temperature was 25 ℃.MAIN OUTCOME MEASURES: Serum concentration of paeoniflorin after administration of Radix Paeoniae Rubra extracts and Taohong Siwu Tang in mice.RESULTS: Seventy-two mice were used for Radix Paeoniae Rubra extr acts and Taohong Siwu Tang tests, and all of them entered the stage of result analysis without deletion. ① Chromatographic isolation results: Paeoniflorin could be well isolated from other components. The linear range for detection of paeoniflorin was 5.40-646.0 μg/mL. The mean recovery rate was 93.6% and the limit of detection was determined as 1.08 mg/L. ②Comparison of concentration of serum paeoniflorin of mice between two groups: The concentration of serum paeoniflorin in the Radix Paeoniae Rubra extracts group and Taohong Siwu Tang group reached the peak at 30 and 60 minutes after intragastric administration, respectively. The peak concentration of paeoniflorin of Taohong Siwu Tang group was lower than that of Radix Paeoniae Rubraextracts group [(36.27±5.72) mg/L vs.(46.82±5.29) mg/L, P ＜ 0.01 )].CONCLUSION: The concentration of serum paeoniflorin is significantly decreased after intragastric administration. It was further confirmed the decreased concentration of the crude paeonifiorin in the mice sera might result from promotion of its metabolic conversion by other chemical components in the complex prescription. This result needs further investigation.

Objective To investigate the efficacy and safety of oxcarbazepine combined with levetirac-etam on children with epilepsy. Methods According to different treatments, 62 cases of children with epilepsy were divided into the OXC group (treated with oxcarbazepine, 40 cases) and the OXC combined with LEV group (treated with oxcarbazepine and levetiracetam, 20 cases). Then the therapeutic efficacy, EEG changes and ad-verse reactions of patients in the two groups were studied. Results The total efficacy was 87.5% in the OXC group, and was 95.5% of the OXC combined with LEV group, with no significant difference. EEG improvement rate was 52.5% in the OXC group and 81.8% in the OXC combined with LEV group , with significant difference (P < 0.05). The rate of adverse reactions in the OXC combined with LEV group was lower than that in the OXC group (P < 0.05). Conclusion Both levetiracetam and oxcarbazepine has good curative effect on children with epilepsy , but combination with levetiracetam can significantly decrease the epileptic discharges on EEG , with higher security.

AIM: Determination of total alkaloids in Dendrobium nobile Lindl.METHODS: Dried materials were subjected to exhaustive extraction with ethanol: water (9:1,v/v) under reflux.Following filtration and condensation,the residue was dissolved in 3% hydrochloric acid and filtered.The solution was refined using a strongly acidic cation exchange resin column through sequential elution.After elution of deionized H2O,the eluent with 50% of alcoholic plus 5% of ammonia was collected until alkaloids were completely desorbed and evaporated under reduced pressure.The test sample was obtained.The test sample was dissolved in glacial acetic acid and titrated with perchloric acid titrand(0.1 mol/L) with automatic titrator.The herb should contain alkaloids,calculated as dendrobium(per 1ml of perchloric acid titrand is equivalent to 26.3 mg dendrobium).RESULTS: The results of three batches of material were 0.85% ,0.63% ,1.03% respectively.CONCLUSION: The method is convenient,fast,effective and reliable.Automatic potentiometric titration can be used for determination of alkaloids in Dendrobium nobile Lindl.

Objective The incidence of pancreatic cancer appears to be increasing and the prognosis is poor. Recent studies have revealed that angiotensin Ⅱ type 1 receptor (AT1) stimulates the growth and angiogenesis of tumor, and selective AT1 antagonist inhibits these effects. This study is investigated the expression of AT1 in pancreatic cancer cell lines. Methods The pancreatic cancer cell lines used in this study were SW1990, PaTu8988s and PANC-1. RT-PCR was used to detect the AT1 mRNA expression, and ABC immunocellularchemical staining and sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) were used to detect the AT1 protein expression. Results Both AT1 mRNA and protein were expressed in the three cell lines. The AT1 protein was found in the cell membrane and cytoplasm of these cells. The AT1 protein ( M r 44?10 3) was also demonstrated by SDS-PAGE. Conclusion The results suggest AT1 may play an important role in pancreatic cancer growth, and inhibition of AT1 might be a useful therapeutic strategy.

OBJECTIVE To study the relationship between HLA-DRB1*03 genes and expression of cytokine IL-18 in chronic hepatitis B patients.METHODS A total of 153 chronic hepatitis B patients and 138 normal healthy people were detected.HLA-DRB1*03 alleles were conducted in all the patients with polymerase chain reaction-sequence specific primer(PCR-SSP),and IL-18 was detected by ELISA.RESULTS Thirty-four individuals with chronic hepatitis B patients were HLA-DRB1*03 genes positive,the frequency was 22.22%,which was higher than the frequency of HLA-DRB1*03 in heathy individuals.The average level of IL-18 in chronic hepatitis B patients with HLA-DRB1*03 negatives was significantly higher than in HLA-DRB1*03 positives.CONCLUSIONS HLA-DRB1*03 closely relates with susceptibility to chronic hepatitis B.In chronic hepatitis B patients,the average level of Th1 cytokines in HLA-DRB1*03 negatives is higher than that in HLA-DRB1*03 positives.