Objective To assess the family environment of neurosis patients and its affect to the prognosis. Methods The gender,knowledge,contact time for each week,yearly income,city/countryside and the factors of FES-CV were compared between the patients" family and normal family and the correction between the relapse and the family factors of the patients were analysed.Results Expressed emotion and intimacy of the patients' family were evidently lower than those of the normal family,but the factor of control the former was higher than that of the latter.Expressed emotion and intimacy were correlative with the relapse.Conclusion Family environment has the important influence on the maintenance and the relapse of the neurosis.

Objective In order to find a more sensitive and specific noninvasive assay for the diagnosis of bladder carcinoma, the authors tested the exfoliated cells from patient′s voided urine for the presence of telomerase activity and evaluated its clinical significance.Methods PCR-ELISA assay was used to determine the presence of telomerase activity in voided urine samples from patients with bladder carcinoma, fhematuria of benign causes and from normal, healthy volunteers.Results Telomerase activity was detected in 14 of 18 tumor samples, while none of the hematuria samples and normal, healthy volunteers′ samples. Conclusions Telomerase activity could be detected in most urine samples of bladder carcinoma patients and it is more sensitive and specific than other assays, therefore, would be a good diagnostic marker.

Objective To investigate the clinical effect by early mechanical ventilation and perfluorocarbons gasification inhala-tion on patients with severe acute pancreatitis (SAP) complicated with acute respiratory distress syndrome (ARDS) .Methods 34 cases of SAP and ARDS patients accepted early mechanical ventilation and vaporization of perfluorocarbon inhalation therapy (ob-servation group) ,and 34 patients received mechanical ventilation for early treatment alone (control group) ,The efficacy ,oxygenation index(PaO2/FiO2 ) and inflammatory cytokines IL-1 ,6 ,8 ,and TNF-a level of two groups were compared .Results The total effi-ciency of the observation group was 94 .12% ,which was significantly higher than the control group that was 76 .47% (P<0 .05) . After treatment ,the arterial oxygen saturation (SaO2 ) ,arterial oxygen pressure (PaO2 ) ,PaO2/FiO2 of the observation group in-creased significantly ,and higher than the control group(P<0 .05) .After treatment ,IL-1 ,6 ,8 ,TNF-αof the observation group were significantly lower than the control group(P<0 .05) .Conclusion The treatment of early mechanical ventilation with perfluorocar-bon inhalation gasification could control the inflammation in patients with SAP complicated with AROS ,which could improve the cure rate .

Peyronie's disease is characterized by local fibrosis of the tunica albuginea and relatively rare clinically. Few relevant basic researches could be retrieved, which might be attributed to the absence of a robust animal model of the disease as well as to its rareness. At present, some animal models available for Peyronie's disease have their own merits and demerits. TGF-β1-induced and Fibrin-induced models are lack of penile curvature and calcification/ossification. A surgical model might be established for the acute phase of the disease. The characteristic of a widespread fibrotic process involving many organs in the spontaneous model is quite different from that of human Peyronie's disease. Therefore, choosing the right model is essential for researches. This paper presents an overview of the animal models of Peyronie's disease, meant to provide some reference for the basic research of the disease.
Animals ; Disease Models, Animal ; Fibrin ; Fibrosis ; Humans ; Male ; Penile Induration ; chemically induced ; pathology ; Penis ; pathology ; Transforming Growth Factor beta1

Humans ; Hypertension, Pulmonary ; metabolism ; Iron ; metabolism ; Metabolic Diseases

China ; Drug Discovery ; Drugs, Chinese Herbal ; pharmacology ; Gastritis, Atrophic ; drug therapy ; Humans

Adult ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Medicine, Chinese Traditional ; Methotrexate ; therapeutic use ; Mifepristone ; therapeutic use ; Phytotherapy ; Pregnancy ; Pregnancy, Ectopic ; diagnosis ; drug therapy

Objective　The aim of this paper was to investigate endothelial dysfunction，platelet activation, and inflammation in elderly hypertensive patients and those complicated with cerebral infarction. Methods　Twenty-eight elderly hypertensive patients complicated with cerebral infarction(within 72 hours after onset of neurological symptoms),thirty-one Stage Ⅰ～Ⅱelderly hypertensive patients and twenty-eight elderly healthy individuals were selected as subjects.Their plasma concentrations of von Willebrand factor(vWF), alpha granule membrane protein-140(GMP-140), and serum concentrations of C-reaction protein(CRP) were examined. Results　Elderly hypertensive patients complicated with cerebral infarction had significantly higher plasma vWF, GMP-140, and serum CRP than elderly hypertensive patients and elderly healthy individuals. Plasma vWF and GMP-140 were higher in elderly hypertensive patients than elderly healthy individuals, whereas serum CRP was increased slightly and there was no significant difference between elderly hypertensive patients and elderly healthy individuals. Conclusion　This study suggests that elderly hypertensive patients complicated with cerebral infarction have obvious endothelial dysfunction, platelet activation, and inflammatory change. Stage Ⅰ～Ⅱ elderly hypertensive patients have obvious endothelial dysfunction and platelet activation as well.

Objective To construct prokaryotic expression vector of NS5A transactivating protein 7 of hepatitis C virus (NS5ATP7), induce the expression of NS5ATP7 in E. coli, and to predict its structure and function by bioinformatics analysis. Methods NS5ATP7 gene was amplified by reverse transcription PCR (RT-PCR) using HepG2 cells mRNA as template, and was ligated into pGEM-T cloning vector. After verifying the sequence of the inserted fragment by restriction enzyme digestion identification and sequencing, NS5ATP7 was cloned into inducible prokaryotic expression vector pET-32a(+) and transfected into competent E. coli BL21. The protein expression was induced with IPTG and the product was analyzed by SDS-PAGE and Western blotting hybridization. The structure and function of NS5ATP7 were predicted using bioinformatics techniques. Results NS5ATP7 gene with about 891bp was amplified by RT-PCR, which was coincident with that we expected, and it was then inserted into expression vector pET-32a(+). Under the induction of IPTG, the recombinant NS5ATP7 was expressed successfully. SDS-PAGE and Western blotting assay showed that this recombinant protein possessed good immunogenicity. Bioinformatics method such as ExPASy, TMHMM and SignalP analysis indicated that NS5ATP7 was full of helices, had neither transmembranous structure nor signal peptide. Conclusions The recombinant NS5ATP7 gene could be successfully expressed in prokaryotic expression system of E. coli, which might lay the foundation of studying the immunogenicity and biological charactersitics of the NS5ATP7. Bioinformatics analysis may provide a new method to analyze the structure and function of a new protein.

Objective To investigate the knowledge degree of the risk of intrauterine adhesions in patients with induced abortion.Methods A total of 360 patients,who accepted induced abortion in gynecological clinic from June to November 2015,were evaluated using self-edited Assessment on Doctor-patient Communication in Gynecological Outpatient Clinic.Results 83.9％ patients could provide previous pregnancy and fertility history,24.2％patients could repeat clearly therapeutic advice and attentions after induced abortion,13.1 ％ patients understood the risk of uterine adhesion,9.7 ％ patients understood the influnce of uterine adhesion on fertility and 31.9 ％ patients wished to prevent uterine adhesion with drugs,patients with spontaneous abortion and multiple abortion history understood the risk with 67.6％ and 31.5％ respectively,overall 61.9％ patients felt the pressure and wished to get more attetions.Conclusion We need to strengthen the risk informing of intrauterine adhesions after induced abortion.