Acute Disease ; Adolescent ; Adult ; Benzene ; poisoning ; Cough ; etiology ; Dexamethasone ; therapeutic use ; Fatigue ; etiology ; Female ; Glucocorticoids ; therapeutic use ; Glutathione ; therapeutic use ; Humans ; Length of Stay ; Male ; Middle Aged ; Occupational Diseases ; etiology ; therapy ; Occupational Exposure ; adverse effects ; Treatment Outcome

Objective To observe the clinical effect of combination of clopidogrel and simvastatin on coro-nary heart disease and transient ischemic attack(TIA). Methods 76 patients with coronary heart disease and TIA were randomly divided into test group (n=40) and control group (n=36). The control group was treated with en-teric-coated aspirin 50 mg×2 every night after supper, and the test group was treated with clopidogrel 25 mg×2 and simvastatin 10 mg×2 every night before sleep. Liver and kidney function, blood coagulation function and blood lipids were measured before treatment and after. 1 year followed-up. Results The effective rate was 95.0% (38/40) in test group and 55.5% (20/36) in control group(χ2=6.45,P<0.01). LDL-C was (3.18±1.24) mmol/L and (2.60±1.03)mmol/L(t=2.67,P<0.01),TC was(5.18±1.24) mmol/L and (4.02±2.18) mmol/L(t= 4.91, P<0.01),TG was (1.50±1.02) mmol/L and (1.30±1.03) mmol/L(t=1.02, P>0.05), respectively in test group before and after treatment. However, there was no statistical difference in LDL-C, TC and TG (t=0.17, 0.00,0.52,0.57,P>0.05 for each) in control group. The two groups showed no difference after treatment (t= 1.51,2.55,0.57, P>0.05 for each). Conclusions Glopidogrel combined with simvastatin capsules is safe in pre-vention of TIA attack.

Objective To test the content of menthol in Chuanbei Pipa Syrup(CPS)by GC, and to evaluate its quality. Meth-ods The content of menthol in CPS was determined by capillary gas chromatography. DB-WAX capillary column (30 m ×0. 319 mm × 0. 50μm) was used with nitrogen as carrier gas. The precolumn pressure was 500 kPa and the column temperature was at 110 ℃ with split ratio of 25 : 1. The temperature was set at 250℃ for sample injection and for the detector. Results The content of menthol in CPS produced by Henan Pharmaceutical Co. , Ltd. , Zhengzhou Pharmaceutical Co. , Ltd. and Yunan Pharmaceutical Co. , Ltd. was 0. 154 mg/mL, 0. 203 mg/mL and 0. 109 mg/mL, respectively. Of them, the men-thol content in CPS produced by Zhengzhou Pharmaceutical Co. , Ltd. met the requirements of Pharmacopoeia(2005 edition), and that of the other two species was slightly lower. Conclusion The methods are convenient, reliable and accurate for the de-termination of menthol content in Chuanbei Pipa Syrup.

Objective To study the influence of hydrocortisone sodium succinct by intravenous infusion or continuous intravenous micro pumping on the vital signs,shock duration,blood glucose and mortality in the patients with severe sepsis secondary shock.Methods Ninety patients with septic shock treated in our hospital from June 2014 to September 2016 were selected and divided into the control group and observasion group by using the random number table method,45 cases in each group.The control group was given hydrocortisone sodium succinct 200mg/d by slow intravenous drip for 2h,while the observasion group was given hydrocortisone sodium succinct 8.33mg/h by continuous intravenous micro pumping.The two groups were treated for continuous 5 d.The blood glucose,hemodynamics and prognosis were compared between the two groups.Results In the observasion group,the blood glucose indicators such as the time window of high blood glucose,LAGE,MBG and CV levels were lower than those in the control group,the difference had statistical significance (P<0.05).The hemodynamic indicators such as HR,CVP and MAP between the two groups before treatment and at 6,24 h and 5 d after treatment had no statistical difference between the two groups (P>0.05).The shock duration,ICU stay length,total hospital stay and 28 d mortality in the observasion group were lower than those in the control group,but the difference between the two groups had no statistical significance (P>0.05).Conclusion Continuous intravenous micro pumping of hydrocortisone sodium succinct can stabilize blood glucose fluctuation range in the patients with septic shock,but the hemodynamic indexes and prognosis have no obvious improvement.

Objective Explore the feasibility of minute cells STR typing.Methods Minute cells which were obtained by micromanipulation method were amplified with MiniFiler kit,and detected by ABI 3130 genetic analyzer.Results Ten cells can be successfully genotyped.Complete genotyping results can be obtained for one,three or five cells but with stochastic effect.Conclusion Instability is observed in minute cells genotyping,so it can not be used in actual case work.Maybe increase the quantity of DNA template can improve the success rate.

0 05). The detective rate of (85 7%) CgA in the P53 protein positive group(21 cases) was significantly higher than that of 54 5% the P53 protein negative group(55 cases). Conclusions The positive rate of CgA was increased with corresponding to the lower down of differentiation grades . Very close relationship is found between the expression of P53 protein and the incidences of CgA positive cell. It suggested that the expression of P53 protein might play an important role in neuroendocrine differentiation in ovarian mucinous tumors.

Objective To study the clinical features of the extra exocrine gland symptoms of primary Sjgren syndrome(PSS) in elderly people. Methods Clinical data of 106 cases of elderly and 120 cases of non elderly PSS were collected and analyzed. Results A total of 203 cases(89 8%, 203/226) manifested symptoms of extra exocrine glands, in which elderly and non elderly groups accounted for 106(100 0%) and 97(80 8%), respectively. The occurrence rates of arrhythma due to hypokalemia, peripheral neuritis(P0 05). Conclusions The common symptoms of PSS in patients, especially the elderly patients, are extra exocrine gland manifestations as the main complaints though the PSS is a systemic disease. These must be differenciated in order to avoid misdiagnosis.

Objective To analyze gerbil plague in northern Ningxia and the monitoring results,to master the plague epidemic dynamics,and to provide the basis for developmenting countermeasures.Methods Monitoring data of gerbil plague focus in northern Ningxia were collect in 2009,counted main host density,rate of dye fleas,flea body index and bacteriology,serology detect and analyzed the epidemic situation.Results An average density of main host was 1.74/hm2,the average rate of infected fleas was 28.60％,and the average rat body flea index was 0.76.Monitoring found 4 plagues from rat plague epidemic,plague bacteria were found in 16 strains,of which gerbils inspection bacteria 10 strains,Meriones unguiculatus 2 strains,the same type cheopis 2 strains,and bald disease fleas 2 strains.Indirect hemagglutination (IHA) was used to test 529 copies of samples,2 copies were found positive,and hemagglutination-positive rate was 0.38％; eight copies were examined by reverse indirect hemagglutination(RIHA),7 material were found positive,and hemagglutination-positive rate was 87.50％.Conclusions In recent years,the disease is active among animals in gerbil plague focus Ningxia.The density of rodents is higher,and local plague epidemic is found in rats.Monitoring efforts should be strengthened and scope of monitoring should be expanded.We should pay close attention to the epidemic dynamics,control the prevalence and spread of animal disease,and prevent the occurrence of human plague.

Objective To compare the mRNA expressions of interleukin (IL)-17 and IL-23 in peripheral blood of patients with psoriasis vulgaris versus healthy individuals,assess the relationship of these parameters with psoriasis area and severity index (PASI) score,and to investigate the therapeutic mechanisms of total glucosides of peony (TGP) for psoriasis vulgaris.Methods Fifty patients with psoriasis vulgaris and 40 healthy individuals were enrolled in this study.Of these patients,42 were treated with TGP of 600-900 mg twice a day for 8 weeks.Blood samples were obtained from all the healthy individuals,50 patients before treatment,42 patients after 4-week treatment,and 23 patients after 8-week treatment.Real-time fluorescence-based quantitative reverse transcription PCR (RT-PCR) was used to determine the mRNA expression levels of IL-17 and IL-23 in the blood samples.The severity of psoriasis was evaluated using PASI score before and after the treatment.Statistical analysis was done by t test,rank sum test,and Pearson correlation analysis using the SPSS16.0 software.Results The IL-17 and IL-23 mRNA expression levels (given in △Ct value) in the patients before treatment were significantly higher than those in the healthy controls (IL-17,-5.32 ± 0.80 vs.2.79 ± 0.76,t =47.71,P ＜ 0.05; IL-23,-5.43 ± 0.68 vs.-3.77 ± 0.86,t =10.38,P ＜ 0.05),and positively correlated with the PASI score (r =0.61,0.52 respectively,both P ＜ 0.05).A significant decrease was observed in the mRNA expression levels of IL-17 and IL-23 as well as PASI score in the 42 patients after 4-week treatment with TGP compared with those before treatment(IL-17,-2.24 ± 0.61 vs.-5.30 ± 0.78,t =20.40,P ＜ 0.05; IL-23,-1.97 ± 0.74 vs.-5.44 ± 0.68,t =21.69,P ＜ 0.05; PASI,5.8 ± 2.7 vs.9.4 ± 4.2,t =4.68,P ＜ 0.05),and in the 23 patients after 8-week treatment compared with those after 4-wek treatment(IL-17,-1.51 ± 0.78 vs.-2.21 ± 0.59,t =3.50,P ＜ 0.05; IL-23,-1.27 ± 0.81 vs.-1.89 ± 0.72,t =2.70,P＜ 0.05; PASI,3.8 ± 1.8 vs.7.3 ± 2.5,t =5.47,P＜ 0.05).Conclusions It seems that both IL-17 and IL-23 are involved in the pathogenesis of psoriasis vulgaris,and TGP treatment can reduce the mRNA expression levels of IL-17 and IL-23 as well as PASI score in patients with psoriasis vulgaris.

Objective To investigate the diagnostic value of EUS with miniprobe on upper gastrointestinal tract mesenchymal tumor(GIMT).Methods The EUS features of 38 patients with GIMT who underwent EUS with miniprobe were studied retrospectively,and the results were compared with the postoperative pathological findings.Results Among 38 GIMT cases detected by EUS,there were 25 cases of gastrointestinal stromal tumor,11 cases of leiomyoma,and 2 cases of leiomyosarcoma.Postoperative histopathological and immunohistochemical examinations confirmed 28 cases of stromal tumor.In which 6 cases were high-risk GIST,8 cases were leiomyoma,1 case was leiomyosarcoma,and 1 case was neurofibroma.The accuracy of diagnosis with EUS was 89%.Conclusion EUS is an accurate method in diagnosis of submucosal tumors,which can make better differentiation diagnosis between GIMT and other submucosal tumors.