Replacement therapy with plasma derived or recombinant F Ⅷ (rFⅧ) concentrates is only effective way to treat hemophilia A.Currently,the first,second and third generation rFⅧ are commercially available.Of the three products,the third generation rFⅧ products are best,because of no additional human or animal plasma proteins at any period.The use of rFⅧ concentrates has greatly improved the safety of replacement therapy in hemophilia A and reduced the risk of blood-borne pathogen transmission.In this article we review the current knowledge about the three rF Ⅷ concentrates,analyzing their main characteristic and their clinical hemostatic efficacy and safety.

A series of innovations in EMP teaching have been carried out in the 7-year-program clinical medicine from such aspects as curriculum design,teaching methodology and strategies,form of examination and establishment of an open teaching system.This paper is to compare the 5 language skills before and after the innovation.

OBJECTIVETo search for an optimal protocol and freezing conditions for the cryopreservation of microamount round spermatids of the mouse.

METHODSWe compared the survival rates of frozen-thawed microamount round spermatids of the mouse achieved by vitrification or standard slow freezing with different concentrations of glycerol (5, 7, or 9%) and different lengths of equilibrium time (0, 15, 30, 45, or 60 min).

RESULTSUnder the conditions of 7% glycerol and 30 min equilibrium, both vitrification and standard slow freezing achieved high survival rates of spermatids, and the former obtained an even higher rate than the latter ([72.9 ± 15.4]% vs [58.2 ± 17.7]%, P < 0.05).

CONCLUSIONA high rate of frozen-thawed microamount round spermatids of the mouse can be achieved by vitrification under the conditions of 7% glycerol and 30 min equilibrium.

Animals ; Cell Survival ; Cryopreservation ; methods ; Cryoprotective Agents ; administration & dosage ; pharmacology ; Glycerol ; administration & dosage ; pharmacology ; Male ; Mice ; Spermatids ; Time Factors ; Vitrification ; drug effects

Objective To study the changes of cerebral haemodynamics in neonates with hypoxic-ischemic encephalopathy(HIE) treated with Shengmai injection compared with citicoline,and explore the clinical interventions of Shengmai injection on HIE.Methods Fifty patients with HIE were randomized into 2 groups: shengmai group treated with Shengmai injection(n=30),and citicoline group treated with citicoline(n=20).The haemodynamic parameters included peak systolic flow velocity(PSFV),end-diastolic flow velocity(EDFV),pulsation index(PI) and resistance index(RI) of anterior and middle cerebral artery were detected by color Doppler ultrasound before treatment and on 7~(th) day after treatment respectively.Control group(n=50) compared with the two groups.Results PSFV,PDFV and RI of HIE patients were significantly lower than those of control group(P

Objective To study the relationship among insulin-like growth factor 1 (IGF-1), insulin-like growth factor binding protein 3(IGFBP-3) and birth weight, length and placental weight and to discuss the endocrine factors which affected fetal growth by assaying levels of IGF-1、IGFBP-3 in umbilical cord serum.Methods The neonates were divided into appropriate for gestational age infant(AGA) and small for gestational age infant(SGA) according to birth weight and gestation. The birth weight, length and placental weight of neonates were measured and at meantime the levels of IGF-1 and IGFBP-3 were assayed by immunoradiometric assay.Results 1.There were 105 neonates, including 79 AGA and 26 SGA. There were significantly statistical differences in birth weight, length and placental weight (all, P

Objective To investigate the morphological change of intratumoral microvessels after administration of thalidomide in the murine hepatic metastases. Methods Among 20 mice with hepatic metastases created by injection of colon-26 tumor cells into the spleen, 10 were treated with thalidomide (200 mg/kg) by intraperitoneal injection daily, the other 10 were treated with saline only by intraperitoneal injection daily. Fifteen days after inoculation of tumor cell, the intratumoral mierovessel of hepatic metastases with similar size in both groups were studied with in vivo microscopy (26 and 27 neoplasms in experimental group and control group respectively ) and immunohistochemistry for CD34 (52 and 55 neoplasms in experimental group and control group respectively). Two-tailed student t test was used to determine differences in intratumoral microvessel density (MVD), intratumoral branch density (BD) and CD34 positive intratumoral microvessel density (MVD-CD34) between the small ( < 400 μm in diameter) and large metastases in both groups, and that between thalidomide treated group and control group. Results For the control group, although the MVD and MVD-CD34 of larger metastases was more than that of small metastases respectively [(18.1±3.5)% vs (13.0±3.2) %, t =2.840,P<0.01;(22.9±2.8)vs ( 12. 8±2. 5) vessels per field, t = 9. 860, P < 0. 01 ], the BD was similar to that of small metastases [(110.0±20.5)vs(99.7±17.3) branches/rnm2, t = 1.040,P >0.05]. For the thalidomide treated group, despite the MVD-CD34 of larger metastases was more than that of small metastases [ ( 17.4±2. 3)vs (11.5±2. 5 ) vessels per field, t = 8. 770, P < 0. 01], the MVD and BD was similar to that of small metastases respectively [(14.7±3.5)% vs(13.2±3.3) %, t =0.826,P >0.05; (95.3±18.3)vs (97. 1±21. 0)branches/mm2,t=0. 347,P>0. 05]. The MVD, BD and MVD-CD34 of small metastases were similar to each other between two groups ( t = 0. 098, 0. 392,1. 190; P > 0. 05 ), however, that of large metastases were significantly lower in thalidomide treated group than in control group ( t = 3. 140,2. 870, 9. 850;P < 0. 01 ). Conclusions Thalidomide exerts antiangiogenic effect on the hepatic metastases with angiogenesis only, and the different vascular components in the tumor vasculature demonstrate variousresponses to antiangiogenic therapy.

Humans ; Liver ; injuries ; surgery

Objective To study the clinical significance of dynamic determination of serum CRP,IL-6 and fibrinogen(Fib) levels in unstable angina pectoris (UAP) patients.Methods 162 UAP patients were selected as UAP group(including 52 cases of low risk group,53 cases in medium risk group and 57 cases in high risk group),58 healthy people were selected as control group.The CRP,IL-6 and Fib contents were observed in UAP patients after cardiac events.Results At discharge,CRP,IL-6,Fib in UAP low risk group were (3.47 ±0.55)mg/L,(10.38 ± 1.57) ng/L,(1.64 ± 0.65) g/L,which were significantly lower than the admission contents [(6.50 ±0.87) mg/L,(14.35 ± 1.22) ng/L,(3.01 ± 0.28) g/L] (t =2.761,2.945,2.118,P =0.028,0.003,0.031).CRP,IL-6,Fib levels in the medium risk group were (6.87±0.75) mg/L,(14.35 ± 1.02) ng/L,(2.26 ± 0.33) g/L,which were significantly lower than the admission contents [(9.21±1.03) mg/L,(19.24±1.58) ng/L,(4.15 ±0.47) g/L] (t =2.985,3.157,3.548,P =0.003,0.001,0.000).CRP,IL-6,Fib levels in the high risk group were (8.71 ±0.84) mg/L,(17.37 ± 1.26) ng/L,(4.67 ±0.85) g/L,which were significantly lower than the admission contents [(12.48 ± 1.24) mg/L,(25.13 ± 1.84) ng/L,(7.28 ± 0.64) g/L] (t =3.472,4.018,5.097,P =0.000,0.000,0.000).Six months after hospital discharge,in CRP,IL-6,Fib elevated groups,the incidence rates of cardiac event were 40.20％,40.35％ and 47.50％,which were significantly higher than those in normal group (5.0％,4.17％,14.29％,x2 =23.655,2.211,14.442,P=0.000,0.000,0.000).Conclusion CRP,IL-6 and Fib levels in UAP patients were significantly higher than those in healthy people,and with remission,contents would be lower,which was the index to reflect the severity of UAP,but its specificity is bad,need to use caution.

Objective To evaluate the clinical value of frozen-thawed blastocyst transfer and the blastocyst derived from frozen-thawed cleavage stage embryo transfer.Methods The data of 5 1 8 cycles in reproductive medicine center of the hospital from Sep-tember 2012 to August 2013 were analyzed retrospectively.According to the frozen-embryos type,all patients were divided into three groups,group A:frozen-thawed blastocyst transfer,129 cycles;group B:blastocysts derived from frozen-thawed cleavage stage embryos transfer,123 cycles;group C:frozen-thawed cleavage embryos transfer,266 cycles.The clinical outcomes of all groups were compared with each other,and the rates of blastocyst formation and cancellation were compared between group A and group B.Re-sults The rates of biochemical pregnancy,clinical pregnancy and embryo implantation in group A(70.5%,61.2%,42.3%)and group B(67.5%,58.2%,40.2%)were significantly higher than group C(53.0%,42.5%,23.1%)(P<0.05);there were no signif-icant differences in the rates of early abortion,ectopic pregnancy,multiple pregnancy among three groups(P>0.05);there were no significant differences in the blastocyst formation rates of the high quality cleavage embryos at D3 in fresh cycles and the frozen-thawed cleavage embryos(62.5%vs.57.7%)(P>0.05)and those two groups were both significantly higher than the poor quality cleavage embryos at D3 in fresh cycles(20.3%)(P<0.05).Conclusion Blastocyst transfer in vitrified-thawed cycles could get rel-atively satisfactory clinical outcomes.There are higher blastocyst formation rate and better clinical outcomes of transfer blastocyst derived from frozen-thawed cleavage embryo.

Objective To evaluate the clinical effect of different doses of leuprorelin acetate in in vitro fertilization-embryo transfer(IVF-ET).Methods From January 2011 to December 2011,the data of 268 patients undergoing IVF and (or) intracytoplasmic sperm injection (ICSI) in Reproductive Medical Center,Clinical College of PLA,Anhui Medical University were studied retrospectively.All the patients were divided into three groups based on with long protocol and controlled ovarian stimulation (COH) including 83cycles with 1.25 mg of leuprorelin in low dose group,68 cycles with 1.88 mg of leuprorelin in high dose group,117 cycles with 1.25 mg of diphereline in control group.The serum follicle stimulating hormone (FSH),luteinizing hormone (LH),estradiol (E2) and progesterone (P) before gonadotropin (Gn)administration on the days 3-5 of the menstrual cycle and on the day of hCG administration were detected,the dose and duration of Gn,number of oocytes retrieved,number of mature oocytes,the rates of fertilization,embryo cleaved,good-quality embryos clinical pregnancy and early miscarriage were compared among three groups.Results There were no significant differences in age,the level of LH and P on the day of hCG administration among three groups (P ＞ 0.05).The level of FSH was (3.8 ± 1.6) U/L in low dose leuprorelin group,(3.1 ± 1.4) U/L in high dose of leuprorelin group and (2.4 ± 1.3) U/L in diphereline group before Gn administration,which reached statistical difference (P ＜ 0.05).The mean length of Gn stimulation were (9.8 ± 1.7) days in low dose leuprorelin group,(10.5 ± 1.8) days in high dose of leuprorelin group and (11.1 ± 1.4) davs in diphereline group,which reached statistical difference (P ＜0.05).The mean dose of Gn was (24 ± 7) in low dose of leuprorelin group,which was significantly higher than (27 ± 9) in high dose of leuprorelin group and (28 ± 7) in diphereline group (P ＜ 0.05).The level of LH was (2.7 ± 1.6) U/L in low dose of leuprorelin group and (2.2 ± 1.0) U/L in diphereline group before Gn administration,which reached statistical difference(P ＜ 0.05).The cancel cycles were 5 in low dose of leuprorelin group,4 in high dose of leuprorelin group and 7 in diphereline group.The number of ovum was (14 ±7) low dose of leuprorelin group,(13 ±6) in high dose of leuprorelin group,(14 ±6) in diphereline group.The rates of fertilization was 66.26％ (758/1144)in low dose of leuprorelin group,67.01％ (589/879) in high dose of leuprorelin group and 68.54％ (1111/1621) in diphereline group,the rates of goodquality embryos was 64.22％ (472/735) in low dose of leuprorelin group,60.50％ (340/562) in high dose of leuprorelin group and 59.59％ (640/1074) in diphereline group,clinical pregnancy was 49％ (38/78) in low dose of leuprorelin group,42％ (27/64) in high dose of leuprorelin group and 50％ (55/110) in diphereline group,early miscarriage was 18％ (7/38) in low dose of leuprorelin group,15％ (4/27) in high dose of leuprorelin group and 15％ (8/55) in diphereline group,which did not show significant differences (P ＞0.05).Conclusions Both 1.25 mg and 1.88 mg leuprorelin acetate could obtain good downregulation effect and clinical outcomes.1.25 mg leuprorelin acetate could decrease patient's costs by reducing Gn dose and duration.