1.The Development of Process Analytical Chemistry
Chinese Journal of Analytical Chemistry 2001;29(4):473-477
The recent development of process analytical chemistry(PAC) in the past years is reviewed. The content includes process measurement, sensor, chemometrics etc. The future of PAC is also discussed. 62 references are cited
3.Effect of pigment epithelium-derived factor on p38MAPK-CREB pathway and fibronectin in high glucose cultured human mesangial cells
Lan GAO ; Jing LI ; Ling GAO ; Hongmin CHEN ; Lian HONG
Chinese Journal of Nephrology 2012;28(3):212-216
Objective To investigate the effect of pigment epithelium- derived factor (PEDF) on p38MAPK-CREB pathway and the expression of fibronectin (FN) in human mesangial cells (HMCs) cultured with high glucose. Methods HMCs were treated with different concentrations of glucose and the osmotic control respectively in the presence or absence of PEDF for 24 h:normal glucose (5.6 mmol/L),24.4 mmol/L mannitol,high glucose (30 mmol/L),high glucose+PEDF(30 mmol/L glucose with 10 nmol/L PEDF,40 nmol/L PEDF or 100 nmol/L PEDF).After samples were collected,the expression of phospho-p38MAPK (p-p38) and p-CREB was assessed by Western blotting,while FN mRNA and protein expression was assessed with RT-PCR and ELISA respectively. Results In contrast to normal glucose and mannitol treatments,the expression of p-p38MAPK,p-CREB and FN increased significantly in high glucose group (all P< 0.01).However,PEDF abolished the up-regulation of p-p38MAPK,p-CREB and FN induced by high glucose (all P<0.05). Conclusion PEDF may inhibit fibrosis through P38MAPK-CREB pathway in diabetic nephropathy.
4.Protective effects of tea polyphenols on polycythemia induced by chronic hypoxia in rats
Hong LUO ; Wenxiang GAO ; Yuqi GAO ; Fuyu LIU ; Peng LI
Journal of Third Military Medical University 2003;0(14):-
0.05).Conclusion TP may have protective effects on chronical hypoxia induced polycythemia in rats.
5.Analysis and treatment of 13 histiocytic necrotizing lymphadenitis cases.
Hong-jun XU ; Ge GAO ; Li-feng AN
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2011;46(7):590-591
Adolescent
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Adult
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Female
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Histiocytic Necrotizing Lymphadenitis
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diagnosis
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therapy
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Humans
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Lymph Nodes
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Male
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Middle Aged
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Neck
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Young Adult
6.Effects of Spirulina's Complex Preparations on Morphologies and Protein Expression of HSP70 inMyocardium,Skeletal Muscle and Liver Tissue of Mice during Prolonged Exhaustive Eexercise
Meiju ZHU ; Shunsheng GAO ; Hong LI ;
Chinese Journal of Sports Medicine 2003;0(05):-
Objective Effect of spirulina's complex preparation(SACP) on morphologies and protein expression of HSP70 in myocardium, skeletal muscle and liver tissue were investigated in the mice during 4-week prolonged exhaustive exercise . Methods Male mice were divided into 5 groups as follow: N(control), M(exercise with moderate intensity), MS(exercise with moderate intensity plus spirulina supplementation), F(exercise with increasing intensity) and FS(exercise with increasing intensity plus spirulina supplementation). Light microscope and electron microscope were used to observe the effect of SACP on morphologies in myocardium, skeletal muscle and liver tissue in mice in group F and group FS. HSP70 protein expression was detected by immunohistochemical method. Results Under the light microscope, edema, degeneration and necroses in myocardium, skeletal muscle and liver tissues were found in the mice of group F, and fragmentation of muscle fiber, cell swelling, mitochondrial swelling and nucleoplasm solution in myocardium, skeletal muscle were found under the electron microscope in the mice of group F. The aforementioned lesions of myocardium, skeletal muscle and liver tissue in the mice of group FS were obviously alleiated. HSP70 protein expression in myocardium, skeletal muscle cells and liver cells in swimming mice was significantly higher than that in control (P0.05). Conclusions SACP had protecting effect against the microtrauma in myocardium, skeletal muscle and liver tissues caused by prolonged exhaustive exercise, and the effect was probably related to the improvement of the protein expression of HSP70 affected by SACP supplementation.
7.Effects of different concentrations of propofol on apoptosis in hippocampal neurons in neonatal rats in vitro
Chinese Journal of Anesthesiology 2012;(11):1350-1352
Objective To investigate the effects of different concentrations of propofol on the apoptosis in hippocampal neurons in neonatal rats in vitro.Methods Primary hippocampal neurons were prepared from newborn Sprague-Dawley rats (aged 7 days) and cultured for 7 days.The neurons were randomly divided into 7 groups (n =8 each):control group (group C),propofol 4,8 and 12 μg/ml groups (groups P1-3),and fat emulsion 4,8and 12 μg/ml groups (groups F1-3).The cells were cultured for 24 h in the culture medium containing propofol 4,8 and 12μg/ml in groups P1-3,respectively.The cells were cultured for 24 h in the culture medium containing fat emulsion 4,8 and 12 μg/ml in groups F1 3,respectively.The cell morphology was examined by microscopy after 24 h culture.The expression of caspase-3 (by immuno-histochemistry) and neuronal apoptosis were detected.The neuronal apoptosis rate was calculated.Results Compared with group C,the neuronal apoptosis rate and caspase3 expression were significantly increased in groups P1-3 (P < 0.05 or 0.01).The neuronal apoptosis rat and caspase-3 expression were increased in a concentration-dependent manner in groups P1-3 (P < 0.05).The neuronal apoptosis rate and caspase-3 expression were significantly lower in groups F1-3 than in groups P1-3 (P < 0.05).There was no significant difference in the neuronal apoptosis rate and caspase-3 expression between groups F1-3 (P > 0.05).The damage to neurons was induced in groups P1-3 and most severe in group P3.Conclusion Propofol can promote the apoptosis in hippocampal neurons in neonatal rats in vitro in a concentration-dependent manner.
8.Analysis of Test Results of Immunoglobulin E and C3 of Patients with Chronic Urticaria
Hong GAO ; Yi QIU ; Xuezhen LI
Journal of Zhejiang Chinese Medical University 2006;0(05):-
[Objective] To observe the sera immunoglobulin E(IgE) and Complement C3(C3)level changes in patients with chronic urticaria.[Methods] To detect sera IgE and Complement C3 content of 60 cases of chronic urticaria patients and 60 cases of healthy persons by chemiluminescence and Immunity Transmission Turbidity.At the same time,we tested sera IgE and Complement C3 content of both penetration needling and drug groups before and after treatment of 60 cases,and anaylysed the correlation of IgE and Complement C3.[Results]Higher level of sera IgE was detected in patients with chronic urticaria(P 0.05);the correlation of IgE and Complement C3 was negative in both groups.[Conclusions]Detecting Sera IgE and Complement C3 level have reference value in the diagnosis and treatment of chronic urticaria.
9.Laparoscopic cholecystectomy combined with intraoperative cholanggiography and endoscopic sphincterotomy for the treatment of cholecystocholedocholithiasis.
Defei HONG ; Junda LI ; Min GAO
Chinese Journal of Minimally Invasive Surgery 2002;0(S1):-
Objective To evaluate the effects of Laparoscopic cholecystectomy (LC) combined with intraoperative cholanggiography (IOC)and intraoperative endoscopic sphincterotomy (IOEST) for the treatment of cholecystocholedocolithiasis. Methods 106 cases with cholecystocholedocolithiasis diagnosed and treated by LC combined with IOC and IOEST were reviewed retrospectively. Results 60 40%(64/106)patients diagnosed cholelithiasis pre-LC were found choledocholithisis; LC combined with IOC and IOEST was successfully performed on 99 of 106 cases(93 40%),and their CBD stones were completely cleared in 98 of 99 cases(99 00%). Six cases(6 1%) were complicated with mild acute pancreatitis, 1 case(0 01%) with duodenal perforation,1 case(0 01%) with bile leakage, and 2 cases(0 02%) with stomach stasis. Conclusions LC combined with IOC and IOEST was a safe ,effective approach to diagnosis and minimally invasive treatment of cholecystocholedocholithiasis.
10.Infarct-related-artery analysis of acute high lateral wall myocardial infarction
Tao HONG ; Penghe LI ; Wei GAO
Chinese Journal of Interventional Cardiology 1993;0(03):-
Objective To study the characteristics of infarct-related-artery (IRA) in acute high lateral wall myocardial infarction and to give clinical clue for deducing the location of coronary artery lesions. Methods Ninety-six patients with acute high lateral wall myocardial infarction (77 males, 19 females, mean age 57.3?10.7 years) were enrolled in the study. They all had infarction for the first time and were confirmed to have single vessel coronary artery disease by coronary angiography. The patients were divided into 3 groups according to the ECG changes at the onset of infarction. The first group (group A) was simple high lateral wall infartion (23 cases). The second one (group B) was high lateral with anterior wall infarction (58 cases, among them 27 cases with anteral-septal infarction and the other 31 with extensive anterior infarction). The third group (group C) was high lateral with lateral wall infarction (15 cases). Lesion characteristics of IRA were analyzed. Results The infarction-related lesions were mainly on left anterior descending arteries in group B (56/58), and on left circumflex in group C (13/15). But those in group A could be on either diagonal (12/23) or left circumflex (11/23). The difference of distribution of IRA among these groups was statistically significant (P