This article was aimed to study the molecular network and bio-function ofBu-Fei-Yi-Shen (BFYS) decoction for chronic obstructive pulmonary disease (COPD) by bioinformatics analysis, in order to provide new ideas for research on pharmacological mechanism of Chinese medicine compound prescription. Components of herbs in BFYS decoction were searched in the databases. Targeted proteins of each component were found from PubChem. Comparison analyses were performed on molecular network, bio-function and canonical pathways by Ingenuity Pathway Analysis (IPA). The results showed that there were 239 target proteins of BFYS decoction. There were 9 molecular networks of BFYS decoction. The top 3 networks' functions were Cellular Development, Energy Production, and Cancer. The top 3 bio-function of BFYS decoction were Cellular Growth and Proliferation, Cell Death and Survival, and Inflammatory Response. The top 3 canonical pathways of BFYS decoction were Cell Cycle:G1/S Checkpoint Regulation, Chronic Myeloid Leukemia Signaling, and Cyclins and Cell Cycle Regulation. It was concluded that the search of target proteins for herbal compounds and bioinformatics analysis by IPA can be used to reveal the molecular network and bio-function of BFYS decoction.

Objective To study the difference in fibrinogen and D-dimer between the patients with ST-ele-vation myocardial infarction (STEMI) and those with normal angiography of coronary artery.Methods 100 patients with STEMI who underwent PCI and 100 patients with normal coronary arteriograms as controls from Jan.2005 to Dec.2007 were studied.Plasma concentrations of fibrinogen and D-dimer were compared.Results There was no significant difference in gender, age, history of hypertension and diabetes and smoking between the two groups.Plas-ma concentration of fbfinogen(Fg) was higher in control group [(2.65±0.68 )g/L ] than STEMI group [(2.38±0.91)g/L] (P<0.05).The square root of plasma concentration of D-dimer was higher in STEMI group [(13.23±5.08) μg/L] than control group [(9.40±5.03)μg/L ] (P<0.01).The square root of the rate between D-dimer and fibrinogen was higher in STEMI group (9.11±4.13 ) than control group (5.92±3.35 ) (P<0.01).Conclusion The levels of fibrinogen in patients with STEMI are significantly lower than that of control group, and D-dimer is higher in the former group than in the latter group, suggesting that fresh thrombosis and secondary fibrinolysis exit in STEMI patients at the acute stage.

Objective:To investigate the effects of pulsed neodymium yttrium aluminium garnet(Nd:YAG)laser in combination with scaling and root planing(SRP)in the treatment of moderate and server chronic periodontitis.Methods:30 patients with moderate or server chronic periodontitis were included in this split-mouth single blind randomized controlled clinical trial.The contralateral teeth with chronic periodontitis in the patients were randomly divided into 2 groups(n =55)and treated by SRP(group A)and SRP +Laser (group B)respectively.At base line,3 and 6 months after treatment.The clinical parameters including periodontal probing depth (PPD),vertical clinical attachment loss(CAL-V),bleeding index(BI)and CBCT data including alveolar bone density(ABD)and height(ABH)were measured.Results:3 months after treatment,the PPD and BI of group B was lower than those of group A(P <0.05).The gained CAL-V of group B was higher than that of group A(P <0.05).6 months after treatment,group B showed higher CAL-V and ABD at the mesial and distal directions than group A(P <0.05).Conclusion:SRP combined Nd:YAG laser is more ef-fective than SRP in the treatment of moderate and server chronic periodontitis.

Objective To study the effects and safety of levocarnitine and shuxuetong in treatment of the patients with chronic renal failure.Methods 94 patients with chronic renal failure were selected as research object,and they were randomly divided into group A(levocarnitine and shuxuetong group) 47 cases and group B(shuxuetong group) 47 cases.Group A were treated with levocarnitine oral solution and shuxuetong,and group B were treated with shuxuetong,then the percentage of effectiveness,incidence of adverse reactions of group A and group B and serum BUN,Scr,Hcy were analyzed.Results The percentage of effectiveness of group A was higher than that of group B,but the incidence of adverse reactions of the two groups had no difference,and the serum BUN,Scr,Hcy of group A and group B before the treatment had no differences(P＞0.05),those of group A and group B after treatment were compared there were significant differences(all P＜0.05).Conclusion The effects of levocarnitine oral solution and shuxuetong in treatment of patients with chronic renal failure were better.

ObjectiveTo analyze the association between single nucleotide polymorphisms (SNPs) in DISC1 gene.MethodsThe studied sample consisted of 528 patients with schizophrenia (264 males and 264 females) and 528 healthy controls (264 males and 264 females).Two function SNPs were selected and gcnotyped by Illumina Golden Gate assay.Genotype and allele frequencies were compared between patients and controls to assess the association to schizophrenia.The clinical features of the hospital first patients were further examined with the Positive and Negative Syndrome Scale (PANSS) before trcatment with antipsychotics.ResultsTwo SNPs were genotyped in subjects.A significant association was found between rs3737597 and schizophrenia in genotype (387:133:8,350:164:14) and allele frequencies (907:149,864:192,P＜0.05 ).Moreover,the haplotypes,A-A constructed from two SNPs showed significant differences between cases and controls ( P＜ 0.05).For rs821616,there were significant difference in positive syndrome score,delusions and poor rapport between patients with genotypes AA or not.ConclusionThis study describes a positive association between DISC(1) and schizophrenia in the Han Chinese population and DISC1 is the susceptible gene in schiz ophrenia.

Objective To survey the thyroid-hormone changes in gravidas,and to establish the reference intervals for thyroid-hormones in each trimester of normal pregnancy in Pingdingshan.Methods 2 645 gravidas who established documents in our hospi-tal were enrolled,The gravidas were divided to different groups by age (≤30 and >30 years old)and 425 cases of early pregnancy women,1 657 cases of middle pregnancy women and 563 cases of late pregnancy women were collected as research group.4 kinds of thyroid functions in the six groups were detected by electrochemical luminescence.Results FT3 and FT4 gradually reduced as the growth of the pregnancy period,but TSH and TPOAb increased.There is statistical difference in all thyroid-hormones between all trimesters of pregnancy and different age groups.Conclusion There is significant difference in thyroid-hormones for different sta-ges of pregnancy and different age groups.Therefore,establishing reference intervals of thyroid-hormones during different stages of pregnancy may be important for clinical practice.

Objective To research the effect of Bifid Lriple Viable on validity for adjuvant treating ulcerative colitis,colon mucosa IgA and sera immunity indexes in patients with ulcerative colitis.Methods The patients with ulcerative colitis in our hospital from November 2004 to June 2006 were randomized into experiment group and control group.The patients in experiment group were treated with Bifid Lriple Viable and routine treatment,however the patients in control group received only routine treatment.The clinical symptom score,colon mucosa inflammation score,colon mucosa IgA expression,sera T lymphocyte subgroup,sera immunoglobulins and complement C3 and C4 were compared between two groups before treatment and two months after treatment.Results The clinical symptom score and colon mucosa inflammation score and colon mucosa IgA expression,sera T lymphocyte subgroup,sera immunoglobulins and complement C3 and C4 had no significant difference between two groups before treatment.Two months after treatment,the clinical symptom score and colon mucosa inflammation score descended significantly(P0.01),but the ascending value of ratio of CD4+ to CD8+ T lymphocyte was more in experiment group than control group(P

Aim To investigate the antagonistic action of EGCG on apoptosis of rat PC12 cell induced by MPP+.Methods PC12 cells were cultured and the apoptosis induced by MPP+(900 ?mol?L-1)was observed.The cells were randomly divided into 6 groups:blank group without any treatment,MPP+ control group,vitamin E group and EGCG groups(10,50,100 ?mol?L-1).After treatment of drugs,cell viability,leakage of LDH,morphological changes of mitochondria and apoptosis were detected by MTT,Hoechst 33342 staining,transmission electron microscope and flow cytometry,respectively.Results After treatment of cultured PC12 cells with MPP+,cell viability was decreased,leakage of LDH and apoptotic rate were increased,and mitochondria swelling,vacuole and cristae breakage were observed.Vitamin E and EGCG en-hanced cell viability,reduced the leakage of LDH and apoptotic rate,and decreased the damage degree of mitochondria.Conclusions EGCG possesses the ability of inhibiting rat PC12 cell apoptosis induced by MPP+,and its protective action may relate to its function of keeping mitochondria integrality.

Objective:To evaluate the effects of different denture cleaners on the discoloration of heat-cured denture base resin and artificial teeth.Methods:40 same specifications of the heat-curing denture base resin and 40 artificial central incisors were immerced in an acombination stain of coffee,tea and soy sauce for 4 weeks.Then specimens and artificial teeth were randomly distributed into 4 groups and soaked in Polident,Steradent,0.5％ sodium hypochlorite solution and distilled water for 4 hours respectivelly(n =10).Color differences(△E) were measured by using a colorimeter and a denture spectrophotometer before and after staining,and after cleaning.Results:Before or after staining there was no difference of △E among the groups of denture base risn or artificial teeth(P ＞ 0.05).After cleaning the denture base resin and the artificial teeth in the group of 0.5％ sodium hypochlorite solution presented higher △E than the other groups(P ＜ 0.05),in group of distilled water presented lower △E than the other groups(P ＜ 0.05).No significant difference was found between Polident group and Steradent group(P ＞0.05).The △E of the denture base resin and artificial teeth.in distilled water group before staining and after cleaning were the highest among the groups(P ＜0.05).Conclusion:0.5％ sodium hypochlorite solution,Polident and Steradent are effective in removing the discoloration from the heat-cured acrylic resin and artificial teeth.0.5％ sodium hypochlorite solution is the most effective,Polident and Steradent are the similar.

Objective To investigate the long-term efficacy of transendocardial injection of vascular endothelial growth factor (VEGF) into ischemic porcine myocardium.Methods Thirty pigs were divided into two groups:control group and treatment group (15 each).A angioplasty balloon was used to occlude the left anterior descending coronary arteries of the animals to reproduce a porcine myocardial ischemic model.Blank plasmid (control group) or constructed pIRES2-EGFP-hVEGF165 eukaryotic expression plasmid (treatment group) was directly injected into the ischemic myocardium through an transendocardial injection catheter of NOGA system,respectively.Before and one year after injection,left ventricular electromechanical mapping (LVEMM) was used to detect local linear shortening (LLS) and unipolar potential (UpV);M-mode local wall motion,cycle variation of integrated backscatter (CVIB) and left ventricular ejection fraction (LVEF) were detected by ultrasonagraphy.One year later,the animals were sacrificed for the observation of the capillary formation in myocardial tissue with immunohistochemical staining.Results One year after injection,as compared with that of control group,local linear shortening (LLS) showed a significant increase (P0.05),and ultrasonagraphy showed better heart function in the treatment group (P