Objective To investigate the clinical effect of inhaled ribavirin on the therapeutic actions and the expression of interleukin 6 (IL -6),interleukin 10 (IL -10)and CysLTs in children with RSV bronchitis.Methods 81 cases of children with RSV bronchitis,were chosen and randomly divided into observing group and control group. Two groups of children were inhaled pulmicort respulas and accessed others support treatment,until the symptoms dis-appeared.But observing group were added with ribavirin atomization.Treatments time and symptoms disappearance time in the hospital were compared,and the serum IL -6,IL -10 and CysLTs level changes before and after treatment were observed.Results Treatments time and symptoms(wheezing,coughing,dry and wet rale)disappearance time in observation group were shorter than those of the control group with (6.58 ±1.36)d vs.(8.92 ±1.12)d,(1.21 ± 0.59)d vs.(2.68 ±1.22)d,(4.86 ±1.31)d vs.(7.34 ±1.39)d and (4.00 ±1.52)d vs.(6.37 ±1.58)d,which with significant differences (t =-8.387,-7.012,-8.267,-6.887,all P <0.01 ).Before the treatment,two groups had no significant difference (P >0.05 ).After treatment,the levels of serum IL -6 and CysLTs were significantly lower in the observation group,and the levels of IL -10 were higher with (76.59 ±10.77)ng/L vs. (116.81 ±10.81)ng/L,(34.40 ±7.19)ng/mL vs.(48.26 ±8.36)ng/mL and,(43.25 ±3.97)ng/L vs.(31.39 ± 4.81)ng/L.The significant differences were observed in two groups (t =-16.74,-8.024,12.315,all P <0.05). Conclusion Compared with general therapy,the curative effect was significant.Combined inhaled ribavirin and pul-micort respulas in RSV bronchitis early,can reduce patients'clinical symptoms and the treatment time.

Objective To investigate the clinical and pathological manifestation of acute kidney injury ( AKI) following infusion of clindamycin .Methods 12 patients were diagnosed as the infusion of clindamycin induced AKI .The clinical and pathological manifestations of these patients were investigated .Results 8 patients (66.7%) had episodes of gross hematuria .Oliguria and anuria was in 6 patients(50.0%).The histological diagnosis of acute interstitial nephritides(AIN) included 5 patients(83.3%).The immunofluorescent examination was negative in all cases .Renal replacement therapy were delivered to four patients .Prednisone was prescribed to 7 patients .All of patients discharged from the hospital and free of renal replacement therapy .The level of serum creatinine decreased to normal 6 months later .Conclusion Most of the AKI associated with clindamycin was oliguria with episodes of gross hematuria ,while the manifestations of skin rash were uncommon .The histological changes revealed AIN .The recent prognosis was relatively good ,but the long-term prognosis should be investigated .

Desensitization, Immunologic ; Humans ; Rhinitis, Allergic, Perennial ; therapy ; Rhinitis, Allergic, Seasonal ; therapy

Child ; Guidelines as Topic ; Humans ; Invasive Pulmonary Aspergillosis ; diagnosis ; therapy ; Lung Diseases, Fungal ; diagnosis ; microbiology ; therapy

Child ; Chronic Disease ; Cough ; diagnosis ; Humans ; Practice Guidelines as Topic

Chronic Disease ; Consensus ; Heart Failure ; diagnosis ; therapy ; Humans ; Integrative Medicine

Objective To investigate whether insulin glargine can increase compliance and improve hyperglycemia or not in solitary senile patients with type 2 diabetes.Methods Thirty-two solitary senile patients with type 2 diabetes were given insulin glargine once daily besides the previous oral hypoglycaemic agents.Results FBG decreased(2.6? 1.5)mmol/L and(0.3?0.1)mmol/L in observing and control group respectively(t=1.691,P

AIM: To investigate the influence of tumor necrosis factor-α (TNF-α) induced apoptosis through knocking down Bax gene by RNA interference (RNAi) in human alveolar type II epithelial cells (A549). METHODS: A549 cells were cultured in vitro and divided into 4 groups according to RNAi treatment: control group, TNF-α treated group, Bax siRNA group and control siRNA group. Chemically synthesized small interfering RNA (siRNA) directed against human Bax gene was transfected into A549 cells by cationic liposome. The effect of RNAi was investigated by reverse transcription PCR, Western blotting and immunohistochemisty, and the rate of apoptosis was investigated by flow cytometry. RESULTS: Bax gene was knocked down effectively in Bax siRNA group (P<0.05). Apoptosis was induced by TNF-α in TNF-α treated group and control siRNA group. However, it was abolished in Bax siRNA group by the downregulation of Bax gene compared to TNF-α treated group and control siRNA group (P<0.05). CONCLUSION: This result suggests that Bax gene has a significant role of pro-apoptosis in A549 cells and knocking down Bax gene by RNAi can effectively inhibit TNF-α-induced apoptosis in A549 cells.

AIM:To investigate the influence of tumor necrosis factor-? (TNF-?) induced apoptosis through knocking down Bax gene by RNA interference (RNAi) in human alveolar type II epithelial cells (A549). METHODS: A549 cells were cultured in vitro and divided into 4 groups according to RNAi treatment: control group,TNF-? treated group,Bax siRNA group and control siRNA group. Chemically synthesized small interfering RNA (siRNA) directed against human Bax gene was transfected into A549 cells by cationic liposome. The effect of RNAi was investigated by reverse transcription PCR,Western blotting and immunohistochemisty,and the rate of apoptosis was investigated by flow cytometry. RESULTS: Bax gene was knocked down effectively in Bax siRNA group (P

Eukaryotic elongation factor 2 kinase (eEF2K) is well known as a Ca2+/calmodulin (CaM)-dependent kinase. eEF2K catalyzes the phosphorylation of eEF2 and subsequently inactivates eEF2 by impairing its ability to bind to the ribosome, thereby negatively modulates protein synthesis. The high expression of eEF2K has been found recently in several types of malignancies. As participating in the progress of tumor, eEF2K emerges a potential target for future cancer therapy. The relationship between eEF2K and tumor, and the latest progress of eEF2K inhibitors were summarized in this article.
Elongation Factor 2 Kinase ; antagonists & inhibitors ; metabolism ; Humans ; Neoplasms ; metabolism ; Peptide Elongation Factor 2 ; metabolism ; Phosphorylation