Objective To investigate the characters of TCM syndromes of HIV/AIDS patients by intraveneous drug. Method Based on the TCM theories,related literature and experts' experience,the questionnaire was compiled and revised,170 patients were investigated one by one,and the data was analysed. Result The frequency lines in turn:spleen qi deficiency,liver depression with qi stagnation,kidney yin deficiency,phlegm-heat congestion of lung,disharmony of liver and stomach,lung qi deficiency,kidney deficiency of both yin and yang,qi deficiency,phlegm-damp congestion of lung,liver fire,heart yin deficiency. The excess syndromes lies in the liver and the lung. The deficiency syndromes lies in kidney,spleen and stomach,and lung. Conclusions The frequent syndromes in the study distinguished from the other congener study. It may be caused by many different contribution factors of patients and researchers.

Objective To explore the sleep problem and its related factors,and to provide evidence for improving sleep quality of patients with ankylosing spondylitis (AS). Methods Pittsburgh sleeping quality index (PSQI),self-rating depression scale (SDS),self-rating anxiety scale (SAS) and a self-designed questionnaire were applied to survey 318 patients with AS.Data were analyzed by the SPSS 17.0 software.The relationship between the various relevant factors were revealed by t-test,x2 test,Wilcoxon rank-sum test and Pearson correlation analysis.Results The mean total score of PSQI was 6.6±3.6, and 35.4％ of AS outpatients showed poor sleep quality.The difference of PSQI scores and detection rate of poor sleep quality between male and female were not statistically significant.There were significant difference of early morning stiffness(Z=-3.077,P=0.002),BASFI(Z=-4.766,P=0.000),BASDAI(Z=-6.880,P=0.000),nocturnal pain VAS(VAS)(Z=-6.502,P=0.000),total back pain VAS(Z=-6.675,P=0.000),ESR(Z=-2.370,P=0.018),CRP(Z=-2.063,P=0.039) between the poor sleep and the good sleep patients (P＜0.05).The PSQI score was positively correlated with age (r=0.165,P=0.003) but not gender and disease duration (r=0.078,P=0.165; r=0.094,P=0.097).PSQI score difference were also observed between patients with negative mood and patients without negative mood (t=-7.613,P=0.000; t=-7.287,P=0.000).Conclusion High incidence of sleep disorder exists among AS outpatients,and it is closely related with age,anxiety,depression and activity of AS.Attention should be paid to AS patients with sleep problems.

Objective To investigate whether unfolded protein response (UPR) plays a role in the pathogenesis of spondyloarthritis (SpA),and to assess UPR-related gene expression in SpA and other arthritis patients.Methods Eighteen patients with SpA,12 with rheumatoid arthritis (RA) and 6 with osteoarthritis (OA) were recruited.Macrophages were isolated from synovial fluid samples by immunomagnetic separation.The expression of UPR-regulated genes,including binding immunoglobulin protein (BiP),glucose-regulated protein 94 (GRP94),C/EBP homologous protein (CHOP),growth arrested and DNA damage-inducible 34 (GADD34),X-box binding protein 1 (XBP-1) and endoplasmic reticulum DnaJ homolog 4 (ERdj4),was tested by real time polymerase chain reaction (PCR).Results Compared with macrophages in OA patients,the expression of BiP and GRP94 mRNA [(6.06 ± 2.08) × 10-2 vs (1.11 ±0.72) × 10-2 for BiP mRNA,11.80(7.30-38.40) × 10-3 vs 1.27(1.02-4.18) × 10-3 for GRP94 mRNA,both P values ＜0.01] was significantly increased in macrophages in SpA patients.XBP1 mRNA was up-regulated [(12.70 ± 5.20) × 10-3 vs (4.14 ± 2.56) × 10-3,p ＜ 0.01] in SpA group as well.UPR-regulated gene expression in SpA patients with HLA-B27 positive or HLA-B27 negative was similar.However,none of UPR-regulated genes showed different expression between the SpA group and RA group except for GADD34 mRNA [7.30 (5.56-15.40) × 10-3 vs 21.30 (12.20-27.60) × 10-3,P =0.009].Conclusions Our data suggest that UPR possibly participates in the pathogenesis of SpA,although the relationship between HLA-B27 and UPR still needs further investigation.

Objective To elucidate that AIDS is not equal to any disease of TCM. Methods Previous literatures on differentiating the difference between AIDS and its similar TCM termed diseases as "Fuqiwenbing", "Wendu", and "Xulao" were reviewed and further discussion on the differences was performed by the author. Results Many scholars draw a conclusion that AIDS is not equal to any of TCM diseases or syndromes by their detailed research, comparison and analysis. Conclusion AIDS is not any of TCM termed disease, but it can be treated with the successful experience of TCM in treating other traditional diseases.

Objective: To evaluate the electron beam computed tomography(EBCT) three-dimensional reconstruction in the diagnosis of bowel obstruction.Methods: Seventy-eight patients suspected of bowel obstruction underwent EBCT with two-phase enhancement scanning and 32 of them were re-established by corona-MPR and sagittal-MPR.All the EBCT results were analyzed and compared with the clinical findings.Results: Seventy-three of the cases were diagnosed as bowel obstruction by EBCT,which were all confirmed by surgery or follow-up,with an accuracy rate of 100% for location diagnosis and 86.3% for etiology.Conclusion: EBCT 3D-reconstruction has great advantages in determining the location,etiology and blood-supply of bowel obstruction.

Objective To introduce a new appointment registration way in the outpatient department.Methods A WeChat appointment registration platform based on HIS was established with smart phone and WeChat public platform,which was gifted with the functions of online registration and payment and etc.The processes including preliminary diagnosis and return visit,cancelling appointment,treatment of appointment breaking,and the limitations during the appointment could be accomplished by the appointment registration.Results The platform realized appointment registration,information feedback and etc.Conclusion The appointment registration based on WeChat public platform contributes to satisfying the patient,enhancing medical service and distributing medical resources rationally.

Objectives To explore the effect of NF-?B signaling pathway on multicellular resistance(MCR) to 5-Fu in colon carcinoma cells.Methods The multicellular spheroids(MCS) of colon carcinoma HT-29 cell line were cultured by using liquid overlay technique,and the monolayer cells by using routine procedure.The morphology of MCS was observed with light microscope and scanning electronmicroscope(SEM).The expression of proliferating cell nuclear antigen(PCNA) was determined by immunohistochemistry.24 hours after adding 50?g/ml SN50 to spheroids cells,the 50% inhibiting concentration(IC50) for 5-Fu was determined by using radial outgrowth assay.The activity of NF-?B was measured by using electrophoretic mobility shift assay(EMSA) and the specific bands were observed.Apoptosis of spheroids cells,which were pretreated with 5?g/ml 5-Fu for 24 hours,was identified by TUNEL,the apoptosis related proteins caspase-3,Bcl-2 and Bax were detected by Western blotting and analyzed by semi-quantitative method.Results A necrotic core formed in the centre of MCS,and the peripheral cells were actively proliferating.Compared with the monolayer cultured cells,the activity of NF-?B was increased in MCS.When the activity of NF-?B in spheroid cells was inhibited by SN50,5-Fu readily induced cells to apoptosis(15.75%?1.02% vs 8.71%?0.73%,P

Two systems which covered nearly 1. 1 billion people in China’s social health insurance, namely the New Rural Cooperative Medical Scheme ( NRCMS ) and the Urban Residents’ Health Insurance System ( URHIS ) , have been depended mainly on government support with high percentage of premium subsidies. This paper deals with the current situation and probes into the main subsidy policy problems by making an overall analysis on the policies of 31 cross-country provinces , and in result points out four problems including the long-term mechanism shortage, in-distinct responsibility between governments at different levels, the equity deficiency and the subsidies inefficiency. Finally, the paper concludes with enlightening some corresponding policies and suggestions.

Objective To investigate the value of imaging in the diagnosis of reflex sympathetic dystrophy(RSD).Methods The clinical manifestations and imaging findings of RSD in five patients were analyzed.Results The onset of clinical symptoms of patients was followed by an injury in all cases.Severe pain,swelling,limited range of motion and vasomotor instability were main complaints.Patchy bone demineralization could be found both on plain radiographs and CT.CT scan was superior to plain radiographs in detecting bone demineralization.Increased scintigraphic uptake in the involved extremities were demonstrated in all four patients who examined by bone scintigraphy.Bony erosions which were not easily visible on plain radiographs could be clearly seen on MRI in one patient at early stage.Conclusion Patchy bone demineralization on plain radiographs and CT is the most outstanding imaging finding in RSD.Bone scintigraphy and MRI can help early in diagnosis of the disease.

Objective To enhance the understanding of the clinical features,diagnosis,treatment and prognosis of reflex sympathetic dystrophy (RSD).Method Three patients with RSD in PLA General Hospital were analyzed.Results In the three patients the symptoms appeared following an injury,they had severe pain,swelling,limited range of motion,vasomotor instability,skin changes,patchy bone demineralization,and increased scintigraphic uptake in the peripheral joints of involved extremities.Two of them had shouder hand syndrome and one had obvious skin thickening.The patients showed better responses after the treatrment of physiotherapy,nonsteroidal antiinflammatory drugs,corticosteroids and tricyclic antidepressants.Conclusion Early diagnosis is very important and appropriate treatment must be initiated as early as possible for RSD patients.