Background and purpose:In China, incidence of gastric cancer is high but early diagnosis is low and treatment results remains poor.The role of neoadjuvant chemotherapy for patients has been explored.In this study, we evaluated the effects and safety of preoperative intra-arterial infusion chemotherapy(PAIC) for advanced gastric cancer.Methods:One hundred and five patients with clinic stage-Ⅱ above advanced gastric cancer were submitted to preoperative intra-arterial infusion chemotherapy using the Seldinger technique.The regimen consisted of 5-FU 750 mg/m2, MMC 10 mg/m2, and DDP 60 mg/m2.Seven to ten days after the PAIC, all 105 patients received operation.Other 95 patients underwent surgery directly as control group.The responses by the tumor lesion and chemotherapy toxicity were observed.The long term survival rate were also evaluated.Results:Pathological examination showed that the tumor cells had changed by chemothrapy.Common adverse effects of PAIC were grade Ⅰ-Ⅱ gastrointestinal discomfort and bone marrow depression.No gastric rupture had occurred.Ninety-one cases(86.6%) underwent curative resection.The 1, 3, and 5-year survival rate was 96.2%, 68.6%, and 52.4% respectively.Among 95 patients with control group, sixty-five cases(68.6%) underwent curative resection.1,3,and 5-year survival rates were 88.4%,38.9% and 28.4%,respectively.Conclusion:PAIC is an effective, and safety chemotherapeutic treatment for advanced gastric cancer.It can affer treatment downstage of disease and enhances resectability.It may also improve the prognosis of advanced gastric cancer.

Objective To analyze the quality of life(QOL)of patients with acute stroke,and to explore the effects of early specialized rehabilitation on their QOL.Methods Seventy patients with acute stroke were randomly divided into a rehabilitation group(n =35)and a control group(n =35).The rehabilitation group received specialized rehabilitation treatment while the control group received self-rehabilitation.QOL we evaluated with the short form-36(SF-36)instrument before and after.Results Before treatment,QOL scores in the both groups were much lower than the norms for healthy people.The differences on each dimension of the SF-36 were not statistically significant between the two groups.The SF-36 scores of both groups improved during the experiment,but after 1 month and 6 months the improvement on each SF-36 dimension was significantly better in the rehabilitation group than in the control group.Conclusions The QOL of patients with acute stroke is significantly impaired,but specialized rehabilitation treatment is effective in improving it.

Objective: To investigate the effects of clinical therapeutic and pharmacological actions “Shenkang Oral Liquid” on sexual function failing, senile nocturia. Results: Index of phagocyte, weight of seminal vesicle gland and content of testosterone in experimental rats with yang deficiency were observed by experiment.Results: “Shenkang Oral Liquid” could significantly raise index of phagocyte (k=0.0258?0.012), weight of seminal vesicle gland (24.07?1.99mg/10g) and content of testosterone (363?50.56pmol/mL) in rats with yang deficiency. Conclusion: “Shenkang Oral Liquid” shows enhancement effects on immune and sexual functions.

Objective To investigate the comparability of K + ,Na+ and Cl- measurement between Cobas8000 analyzer and AU640 analyzer .Methods According to the EP9‐A2 document of the National Committee for Clinical Laboratory Standards ,the precision of AU640 analyzer for the detection of K + ,Na+ and Cl- was evaluated ,and the correlation between the detected results of the two analyzers were also analyzed .Results AU640 analyzer were with high precision for the detection of K + ,Na+ and Cl- ,in‐tra‐assay and inter‐assay coefficient of variation of which were both less than 2% .The correlation coefficients of the two analyzers were higher than 0 .975 .Conclusion Cobas8000 analyzer and AU640 analyzer might be with fine comparability for the detection of K+ 、Na+ and Cl- ,both of which could provide accurate detected results .

After the institutional scientific research management at present was analyzed according to the literature investigation, expert consultation, comparative and comprehensive analysis, the following were advanced, including the principles, target, function module and structure module for institutional scientific research management infor-mation system , and realization of its functions using the J2 EE technology-based B/S framework .

Objective To investigate the blood-retinal barrier(BRB)function of porcine retinal pigment epithelial(RPE)cells cultured in vitro. Methods Primary porcine RPE cells were cultured,and the third generation were inoculated in a microporous filter with the filter membrane of polyvinylpyrrolidone(PVP)-free polycarbonate membrane.After 1,2,3 and 4 weeks of culture,the surface of filter membrane was observed by light microscope,and after 2 weeks of culture,the section of filter membrane was observed by light microscope and transmission electron microscope.Transepithelial electrical resistance(TER)was detected and the permeability was measured with fluorescein sodium(FS)and horseradish peroxidase(HRP). Results Primary porcine RPE cells were cultured successfully.RPE cells converged1week after inoculation; 2 and 3 weeks after inoculation,the density of RPE cells did not changed obviously; 4 weeks after inoculation,the density of RPE cells decreased.The characteristics of polarized growth of monolayer were found in RPE cells on the surface of filter membrane; 2 weeks after inoculation,the TER of RPE cells was(97.44?11.36)?/cm~2 which maintained till the 3rd week after inocubation.After incubated for 30 minutes,only 0.27% of FS and 0.17% HRP reached the inferior filter membrane,and the permeability rate of SF with low molecular weight was higher than which of HRP with high molecular weight. Conclusions The filter with PVP-free polycarbonate membrane may be used to set up the model of RPE cells with polarized growth of monolayer and investigate the barrier function of RPE cells.

Celiac disease (CD) is a chronic autoimmune gastrointestinal disorder induced by ingestion of gluten-containing foods.In the past CD was thought prevalent only in Western countries,whereas it has been found common throughout the world and increasing numbers of Chinese patients has been reported recently.However,there are still many deficiencies in management of patients with CD in China and either the diagnosis or the treatment of CD is lacking evidence-based clinical pathway.We report a case of a young woman with CD presenting with chronic diarrhea,abdominal distention,and weight loss,whose outcome was improved by following evidence-based guidelines of medical nutrition therapy.Diagnosis and treatment of the patient was analyzed with review of foreign and domestic literature to provide readers reference of the standardized nutritional intervention of CD.

This paper introduces the automatic control system of leechdom management in hospital by computer.It can enhance the managerial level of the medicine and reduce the cost,so the managerial level in hospital can be raised through it.

[Objective]To evaluate the effect of bone mesenchymal stem cells(BMSCs) on healing of tendon-bone junction in a bone tunnel and to provide experimental evidence of labeling and tracing the stem cell.[Method]BMSCs from rats were harvested by adherent separation screening method and labeled by SPIO and DiI.Thirty-nine 8-week-old rats were randomly divided into two groups,21 rats injected with double-labeled BMSCs and Pluronic F-127 as experimental group and 18 rats injected with Pluronic F-127 alone as control group.In each group,biomechanical analysis was carried out to assess the effect of healing at 2,4 and 8 weeks.The transplanted BMSCs were traced by fluorescence microscope at 2,4,8 weeks and 7.0T MR instantly,3 and 7days after operation.[Result]BMSCs were labeled effectively by SPIO and DiI.DiI-labeled positive cells were observed between tendon and bone with fluorescence microscope at 2,4 and 8 weeks.No significant signal changes of tendon-bone interface could be observed by 7.0 T MR.Biomechanically,maximum pullout load had no statistical difference between experimental group and control group at 2 weeks.At 4 and 8 weeks,the experimental group had significantly higher maximum pullout load compared with control group(P

Objective To explore the significance of PCNA and ICAM-1 expression in cervical carcinoma. Method Pre-treatment biopsy was conducted and immunohistochemical method was used after treatment to determine the expression of PCNA and ICAM-1 in the tissues of 43 patients with cervical carcinoma. Results The post-treatment evaluation was based on WHO standard. The section staining showed that 38.2% of the CR and PR patients was negative(―) and 31.3% was positive plus() in terms of PCNA expression while none of the NC and PD patients was found negative(―)and 77.8% was found positive plus(). The expression difference was significant(P