1.Diversity of Endophtic Bacteria Isolated from Glycyrrhiza
Min ZHANG ; De-Long SHEN ; Xiao-Li RAO ; Feng-Ming CAO ; Xin JIANG ; Jun LI ;
Microbiology 1992;0(04):-
120 strains of endophytic bacteria identified by ERIC-PCR were isolated from wild and cultivated Glycyrrhiza uralensis plants which collected from Erdos Innermongolia province.The identified results indicated that Glycyrrhiza uralensis plants has plenty of endophytic bacterium in density and population,and the density is higher in root and leave than in stem.Partial sequence analysis of 16S rDNA gene of 82 strains indicated that these strains were in a high similarity with 19 known genus which belong to?、?、 ?-Proteobacteria、Firmicutes and Actinobacteria.The dominant genus were Bacillus sp.,Pseudomonas sp., Pantoea sp.and Serratia sp..
2.Jeune syndrome in a case.
Jia-xin ZHUANG ; Li-de RAO ; Kun-ming WANG ; Qiang WANG
Chinese Journal of Pediatrics 2003;41(9):696-696
Abnormalities, Multiple
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diagnosis
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therapy
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Asphyxia
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pathology
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Female
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Humans
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Infant
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Musculoskeletal Abnormalities
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diagnosis
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therapy
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Syndrome
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Thorax
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abnormalities
3.Antimicrobial resistance of Escherichia coli isolated from different samples in children.
Hai-Jiao ZHU ; Guan-Li RAO ; De-Zhong ZHANG
Chinese Journal of Contemporary Pediatrics 2010;12(5):348-350
OBJECTIVETo investigate the possible differences in antimicrobial resistance of Escherichia coli isolated from different samples in children.
METHODSSix hundred and twenty-nine samples from urine, sputum, blood and secretion were collected from June 2004 to May 2009 for bacterial identification by VITEK-32 automatic system and antimicrobial susceptibility tests by Kirby-Bauer method. The drug resistance rate of Escherichia coli isolated from different samples was compared.
RESULTSTwo hundred and sixty strains of Escherichia coli were isolated , and 108 of which were from urine , 64 from sputum, 54 from secretion and 23 from blood. ESBLs were detected in 96 (36.9%) of the 260 isolates, AmpC enzymes in 32 (12.3%), and ESBLs+AmpC in 8 (3.1%). The ESBLs positive rate of Escherichia coli isolates from sputum was significantly higher than that from other samples (P<0.05). The antimicrobial resistance rate of Escherichia coli strains from different samples to amoxicillin/clavulanic acid, ticarcillin/clavulanic acid, piperacillin, cefotaxime, cefuroxime, cefepime, gentamicin, cotrimoxazole, and nitrofurantoin was different. The resistance rate of the strains from sputum samples was higher than that from the other samples (P<0.05).
CONCLUSIONSEscherichia coli isolated from different samples have different antimicrobial resistance rates in children, so the selection of antibiotics for infections confirmed by bacterial cultures from different samples should based on drug sensitivity results.
Adolescent ; Child ; Child, Preschool ; Drug Resistance, Bacterial ; Escherichia coli ; drug effects ; enzymology ; isolation & purification ; Female ; Humans ; Male ; beta-Lactamases ; analysis
5.Isolation of Endophytic Bacteria from Glycyrrhiza and Identifying of Antagonistic Bacteria
Xiao-Li RAO ; De-Long SHEN ; Jun LI ; Xin JIANG ; Li LI ; Min ZHANG ; Rui-Hua FENG ;
Microbiology 1992;0(04):-
98 endophytic bacteria strains were isolated from different internal tissues of Glycyrrhiza uralensis plants collected from Innermongolia region. Results indicated that the population densities of endophytic bacteria ranged from 5.0?104cfu/g~2.9?107cfu/g fresh weight although it varied depending on tissue of the plant. Among these strains, Bacillus sp. was the most prevalent endophytic bacterium, which was amount to 30%.Of the 98 isolates, 6 strains exhibited extensive antagonistic activities against pathogenic bacteria. Characterization showed that these bacteria were Bacillus atrophaeus、Paenibacillus polymyxa、Bacillus subtilis、Paenibacillus ehimensis. This study indicated that selected 6 endophytic bacteria strains have potential for biological control of plant disease.
6.Report on initiating clinical research for electrical and mechanical synchronism of selective region pacing in the right ventricular.
Lin CAI ; De-jia HUANG ; Chun-bo YAN ; Li RAO ; Jian-xiong LIU ; Han-xiong LIU
Chinese Journal of Cardiology 2007;35(2):147-150
OBJECTIVETo verify the electric synchronism, mechanic synchronism and hemodynamics of selective site pacing.
METHODSPacing in the right ventricular cardiac apex (RVA), the right ventricular His bundle region (His), and the septum of right ventricular high-positioned outflow tract (RVOT), CO and CI were recorded. The electrical synchronism was assessed by observing the width and shape in a 12-lead surface ECG. The mechanical synchronism was estimated by using the VVI (vector velocity imaging) technology of the Acuson Sequia 512.
RESULTSThe results showed that CO and CI were lower while pacing in RVA, but they were not significant different (P>0.05). The QRS width: (124 +/- 5.3) ms while pacing in His, (144 +/- 7.1) ms while pacing in RVOT and (156 +/- 8.6) ms while pacing in RVA. The QRS width while pacing in His and in RVOT were narrower than in RVA and there were significant differences (P<0.01). Vector velocity imaging showed that mechanical synchronism was better while pacing in RVOT than that in RVA.
CONCLUSIONPacing in RVOT seems better than pacing in traditional RVA, and the operation was no more difficult than the traditional operation.
Adult ; Aged ; Bundle of His ; physiopathology ; Cardiac Pacing, Artificial ; methods ; Electrocardiography ; Female ; Heart Ventricles ; physiopathology ; Humans ; Male ; Middle Aged ; Pacemaker, Artificial
7.Measurement of the amino acid sequence for the fusion protein FP3 with LC-MS/MS.
Xiang LI ; Xiang-Dong GAO ; Lei TAO ; De-Ning PEI ; Ying GUO ; Chun-Ming RAO ; Jun-Zhi WANG
Acta Pharmaceutica Sinica 2012;47(2):216-222
The amino acid sequence of the fusion protein FP3 was measured by two types of LC-MS/MS and its primary structure was confirmed. After reduction and alkylation, the protein was digested with trypsin and glycosyl groups in glycopeptide were removed by PNGase F. The mixed peptides were separated by LC, then Q-TOF and Ion trap tandem mass spectrometry were used to measure b, y fragment ions of each peptide to analyze the amino acid sequence of fusion protein FP3. Seventy-six percent of full amino acid sequence of the fusion protein FP3 was measured by LC-ESI-Q-TOF with the remaining 24% completed by LC-ESI-Trap. As LC-MS and tandem mass spectrometry are rapid, sensitive, accurate to measure the protein amino acid sequence, they are important approach to structure analysis and identification of recombinant protein.
Amino Acid Sequence
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Chromatography, High Pressure Liquid
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Molecular Sequence Data
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Peptide Mapping
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Recombinant Fusion Proteins
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chemistry
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Spectrometry, Mass, Electrospray Ionization
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Tandem Mass Spectrometry
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Vascular Endothelial Growth Factor A
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antagonists & inhibitors
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chemistry
8.The inhibitory effect of matrine on the growth of human colorectal cancer HT29 cells: an experimental observation.
Cheng CHANG ; De-Li RAO ; Xiao-Ming QIU ; Hong WANG ; Li XIONG
Chinese Journal of Integrated Traditional and Western Medicine 2014;34(1):62-65
OBJECTIVETo study the inhibitory and inductive effect of matrine (MA) on human colorectal cancer HT29 cells.
METHODSMTT assay was used to determine the cell growth inhibitory rate in vitro . Changes of cell cycle and the apoptosis of HT29 cells before and after MA treatment were observed using flow cytometry and electron microscope.
RESULTSMTT showed that 2-32 mg/mL MA inhibited the proliferation of HT29 cells (P < 0.05) in a dose- and time-dependent manner. Cells treated by 4, 8, and 16 mg/mL MA at G0/G1 phase were obviously higher than those in the negative control group (P < 0.05), indicating that the cell cycle was arrested at G0/G1 phase. Morphological apoptosis of HT29 cells could be seen under transmission electron microscope.
CONCLUSIONMA inhibited the proliferation of HT29 cells, and its mechanism might be associated with stagnation at G0/G1 phase and inducing apoptosis of HT29 cells.
Alkaloids ; pharmacology ; Apoptosis ; drug effects ; Cell Proliferation ; drug effects ; HT29 Cells ; Humans ; Quinolizines ; pharmacology
9. Piezol involved in electrical remodeling in atrial myocytes induced by high hydrostatic pressure through Src kinase
Yuan FANG ; Qian LI ; Guan-Hao LUO ; De-Wei PENG ; Lu-An LI ; Qiao-Qiao LI ; Xue-Shan LUO ; Chun-Yu DENG ; Yu-Mei XUE ; Fang RAO ; Shu-Lin WU
Chinese Pharmacological Bulletin 2022;38(3):422-428
Aim To investigate the role of mechano- sensitive ion channel Piezol in regulating electrical re-modeling of atrial myocytes induced by hypertension and to further explore the potential mechanisms.Methods Spontaneously hypertensive rats ( SHR ) aged 30 - 32 weeks treated with or without valsartan (30 mg • kg 1 • d 1 ) were used.Wistar rats were used as control.Western blot was used to detect the protein expression of Piezol , Src and Cavl.2 in atrial appendages of rats and in atrial myocytes ( HL-1 cells) exposed to different levels of high hydrostatic pressure (20 and 40 mmHg) , Piezol inhibitor (GsmTx4) and agonist ( Yodal ) in vitro.Whole-cell patch clamp technique was employed to detect L-tvpe calcium current (ICa, ) and action potential duration ( APD) of atrial myocytes.Results Compared with Wistar rats in control group, the protein expressions of Piezol and Src significantly increased and the expression of Cavl.2 decreased in SHR group (P < 0.05 ), while the a- bove changes could he reversed in SHR treated with valsartan( P < 0.05 ) .Meanwhile, higher hydrostatic pressure (40 mniHg) could increase the expressions of Piezol and Src in HL-1 cells( P <0.05) and decrease the protein expression of Cavl.2 (P <0.05 ) , accompanied by a shortened APD and a decreased ICa,.GsmTx4 could significantly reverse the above changes.In addition, Piezol agonist Yodal could simulate electrical remodeling and related signal molecule changes in atrial myocytes induced by the high hydrostatic pressure.Conclusions Mechanosensitive ion channel Piezol participates in electrical remodeling induced by hypertension via activating Src kinase signaling pathway and then leading to the decrease of ICa ,.
10. Sacubitril/valsartan reduces susceptibility of atrial fibrillation by reversing structural remodeling of atrial in spontaneously hypertensive rats
Qian LI ; Xin LI ; Lu-An LI ; Hui-Shan ZHOU ; De-Wei PENG ; Chun-Yu DENG ; Fang RAO ; Shu-Lin WU ; Qian LI ; Xin LI ; Lu-An LI ; Hui-Shan ZHOU ; De-Wei PENG ; Meng-Zhen ZHANG ; Zhao-Yu WANG ; Su-Juan KUANG ; Chun-Yu DENG ; Fang RAO ; Shu-Lin WU
Chinese Pharmacological Bulletin 2021;37(5):631-637
Aim To observe the effects of sacubitril/valsartan (Sac/Val, LCZ696) on atrial remodeling and atrial fibrillation (AF) susceptibility in spontaneously hypertensive rats (SHR). Methods Twenty-four 7-week-old male SHR were randomly divided into SHR group, SHR + Val group (30 mg · kg