The Wnt/β-catenin signal transduction pathway is essential for regulating cell proliferation, movement, differentiation and embryo development. Researchers have found that Wnt pathway is an important regulation factor in the early stimulation of the cardiogenesis and contacts closely the several key events during cardiac morphogenesis. The article reviews the recent studies about the relation between cardiac development and Wnt pathway in early embryo.

The Wnt/?-catenin signal transduction pathway is essential for regulating cell proliferation,movement,differentiation and embryo development. Researchers have found that Wnt pathway is an important regulation factor in the early stimulation of the cardiogenesis and contacts closely the several key events during cardiac morphogenesis. The article reviews the recent studies about the relation between cardiac development and Wnt pathway in early embryo.

Objective Based on the result that a gene coding for human HSS had been identified and prokaryotically expressed, this study is aiming to investigate the proliferative and protective effect of recombinant HSS on hepatoma cells. Methods hHSS protein was produced in BL\|21 strain of E.Coli containing pET\|42a vector and purified with His\5Tag affinity chromatography. A various dosages of 80\|400??g/L were administrated into cell culture. The cell proliferation was analyzed by MTT, cell\|count and flow cytometry methods. Furthermore, cellular growth signaling as indexed by phosphorylation of mitogen\|activated protein kinase (MAPK) was determined with Western blot. In addition to cell growth, protection of hHSS on the cells against H\-2O\-2 was also observed. Results It was showed that recombinaht HSS of 400??g/L was able to promote DNA synthesis by 21\^5% as compared to non\|treated cells. After 24?h of HSS action, the cell division measured by MTT method as well as cell\|count was significantly enhanced. Meantime, it was indicated that the phosphorylation of MAPK Thr202/Tyr204 was increased in 79\^0% as compared with that of the non\|treated cells. Pretreatment of the cells with hHSS for 12?h prior to H 2O 2 injury preserved the survival of them.Conclusion It is postulated that hHSS is an active protein to stimulate liver proliferation. [

Atrial Flutter ; diagnosis ; etiology ; therapy ; Electrocardiography ; Female ; Humans ; Infant, Newborn ; Infant, Premature ; Treatment Outcome

Since the oral rehydration salts solution(ORS) has been used to treat acute diarrhea of children, there are different kinds of ORS in order to satisfy the need of clinical work. They are the initial WHO-ORS, other substances-supplemented ORS and the reduced osmolarity ORS, each of which has their own efficiency and advantage.

Objective To study the differences between diet induced obesity (DIO) and diet induced obesity resistance (DIO-R) during insulin resistance (IR) in rats. Method Male SD rats were fed by high-fat diet 1 (45 % fat kJ) after adaptation. At 3rd week, rats were divided into 3 groups according to their body weight: rats belonging to the middle 1/3 of their body weights were the control group, and was fed by control diet (10 % fat kJ). The heaviest 1/3 and the lightest 1/3 belong to DIO and DIO-R group, respectively, and were fed by high-fat diet 2 (60 % fat kJ). Five rats were selected randomly from each group and sacarified at 3rd, 6th, 8th, 10th and 11th week. The energy intake, body fat contents, fasting blood glucose (FBG), serum lipid, serum insulin, insulin sensitive index(ISI)and leptin of rats were monitored dynamically. Results DIO rats were significantly different from DIO-R and control rats in energy intake, body fat content, FBG, lipid, serum insulin and leptin. Conclusion Fed by the same high-fat diet, DIO rats are more sensitive than DIO-R rats to become IR.

OBJECTIVE: To evaluate the in vitro MTT assay for determining the drug sensitivity in fresh human tu- mors. METHODS: We tested the samples from 75 patients with gastric cancer, 55 patients with colorectal carcinoma, 55 patients with bladder cancer and 6 patients with carcinoma of tongue. RESULTS: On the average, the MTT sensitive drug in the above - mentioned first three kinds of solid tumors corresponded with the drug used by clinican experience. The response rate in MTT sensitive group was 73. 8% (48/65) while only 34.0% (17/50) in the control group and 0% (0/10) in MTT resistant group. CONCLUSION: MTT assay appears to be useful in exploring the possible anticancer effect of arsenic trioxide on tongue cancer. This test demonstrats that the MTT assay is clinically useful on an individual basis to optimize chemotherapy.

Objective To discuss the effect of preventing postoperative incision fat necrosis by the method of evacuated suture of reducing tension. Methods The method of evacuated suture of reducing tension was used in 158 cases. Age of them was 22～83 years old,mean 52 years old. There were thick fat tissues in the sub-skin of all patients.The fat average thickness was from 4 0～8 0cm.Results The treatment group had 158 cases, of which, lumbar incision had 62 cases, subcostal incision had 5 cases and hypogastric median incision had 91 cases. Among 158 cases,there were 4 cases of incision fat necrosis, 3 cases of incision infection and the others were one-stage healing.The control group had 97 cases. Among them, there were 18 cases of fat necrosis, 4 cases of incision infection and the others are one-stage healing. Conclusions Method of evacuated suture of reducing tension to prevent incision fat necrosis has satisfactory efficacy and worth of clinical popularization and application.

Objective To evaluate the effect of preoperative intramuscular injection of of dextromethorphan in three dosages on fentanyl consumption in postoperative PCA in order to optimize its pre-emptive analgesia dose. Methods 80 ASA Ⅰ-Ⅱ patients underwent spine operations under general anesthesia were randomly divided into four groups, who respectively received intramuscular dextromethorphan 10mg (DM10 group), 20mg (DM20 group), 40mg (DM40 group), or normal saline (CON group) respectively. The PCA fentanyl consumption as well as VAS pain scores were observed 4h, 24h, and 48h after the operation. Results Although there was no significant differences in pain VAS score between the four groups, the fentanyl consumption was significantly lower in groups DM20 and DM40 compared with group CON and DM10. However, there was no significant difference between group DM20 and group DM40. Conclusion Preoperative IM dextromethorphan shows a dose-dependant fentanyl spare effect on postoperative PCA, and a dose of 20mg seems to be optimal for preoperative use.

OBJECTIVE: To design the dosage regimen of aminophylline using reported pharmacokinetic parameters (mean group value). METHODS: The calculation of oral maintain dose, the infusion rate of intravenous drip maintain dose and the loading dose of intravenous drip were introduced. RESULTS: Different dose were required for different pathophysiological conditions insteading of aminophylline 200 mg three times a day (p.o.) for every patient. CONCLUSION: It is recommended that all hospitals which are unable to determine plasma concentration should design dosage regime of aminophylline using pharmacokinetic parameters.