Objective:To investigate the roles of vascular endothelial growth factor C(VEGF-C) and tumor infiltrating dendritic cells(TIDC) in invasion and metastasis of gastric cancer cells and to investigate the relationship between VEGF-C expression and TIDC infiltration in gastric cancer.Methods:Fifty-two patients,who were pathologically diagnosed with gastric carcinoma and received gastrectomy in the First Affiliated Hospital of Chongqing Medical University from 2004 to 2006,were enrolled in this study.The expression of VEGF-C and infiltration of dendritic cells were examined by immunohistochemical staining(S-ABC method),using anti-VEGF-C polyclonal antibody and anti-S-100 polyclonal antibody,and the relationship between them was investigated.Results:(1) In poorly differentiated and well/moderately differentiated cancer patients,the rates of positive of VEGF-C expression were 75.86% and 44.23%,respectively(P 0.05);the rates of serosa invasion in the high density group and low density TIDC patients were 36.36% and 70%,respectively(P

High - risk corneal transplantation immunological rejection is a primary factor affecting the success or failure of the corneal transplantation. As a result, application of immunosuppressive agent in high - risk corneal transplantation rejection reaction occupies an irreplaceable position. However, many local and systemic adverse reactions appeared in the process of application. In order to improve the achievement ratio of the corneal transplantation, experts and scholars at home and abroad have obtained remarkable achievements in dosage form, route of administration and drug combination;meanwhile, a considerable progress was also made in genetic engineering. This paper is a review of prevention and treatment on high - risk corneal graft rejection reaction.

Objective To explore the value of adenosine deaminase (ADA),interferon (IFN)-γand soluble interleukin-2 receptor (sIL-2R) in differential diagnosis of tuberculous and malignant pleural effusion.Method The ADA activity,IFN-γ and sIL-2R levels of 48 patients with tuberculous pleural effusions and 32 patients with malignant pleural effusion were measured by colorimetry and ELISA respectively.Results The ADA activity,IFN-γand sIL-2R level in tuberculous pleural effusion was higher than that in malignant pleural effusion (P < 0.05).According to the receiver operator characteristic (ROC) curve,when 45 U/L was regarded as critical value of ADA,the sensitivity,specificity and accuracy of ADA in diagnosing tuberculous pleural effusion was 82%,91% and 86% ; when 100 ng/L was regarded as critical value of IFN-7,it was 85%,94% and 89%; when 450 μg/L was regarded as critical value of sIL-2R,it was84%,81% and 83%.When combined detection of three targets was done.it was 88%,100% and 94%.Conclusion The combined detection of ADA,IFN-γand sIL-2R can improve sensitivity,specificity and accuracy in diagnosing tuberculous pleurisy.

Carcinoma, Squamous Cell ; surgery ; Head and Neck Neoplasms ; surgery ; Humans ; Neoplasm Recurrence, Local ; prevention & control ; surgery ; Prognosis

Head and Neck Neoplasms ; surgery ; Humans ; Robotics

Objective Endovascular tubular stent grafting has emerged as an alternative therapeutic modality for patients with aortic dissections. However, its application has been limited to the descending thoracic aorta distal to the origin of the left subclavian artery. The initial clinical experience of endovascular branched stent graft repair for aortic arch dissections involving the left subclavian artery was reported. Methods From February 2004 to January 2005, the endovascular branched aortic stent-grafts made by Beijing YuHengJia SciTech Co. were applied in 42 patients with Stanford type B aortic dissection with the entry tears just beyond (

Objective:To explore the long-term effects of physical exercises on penicillin induced recurrent convulsion with learning and memory deficits in developing rata and its mechanism.Method:Fifty-six male SD rats(postnatal days [PD]21)were randomly divided into four groups:control group (CONT1),control plus exercises group(CONT2),convulsion group(EXP1)and convulsion plus exercises group(EXP2) ,rwenty rats were assigned for two control groups,each n=10;the surplus 36 rats were kindled by penicilln onceper d,consecutive 6d for creating convulsion models.Control rats were injected equal amount of normal sodium(NS) into abdominal cavity at the same time.The 20 qualified models of convulsion rats were randomly divided into two EXP groups.On PD39-PD43 and PD61-PD64,subjects of the four groups were tested with Morris water maze,and from PD49 to PD54.the rats of CONT2 and EXP2 were given exercises of running wheels,twice a d for consecutive 6d.The expression of glutamicacid receptor 2(GluR2)in hippocampus was detected. Result:①In the first Morris water maze test,there were significant differences of latency among four groups(F=5.56,P<0.01),and the latency of two EXP groups were significantly longer than two CONT groups(P<0.05);In the probe trial,the frequency of two EXP groups passing through target area were significantly less than that of two CONT groups.②In the second water maze test.there were significant effects of exercises on the latency of EXF2 group comparing to EXP1 group (P<0.05);the frequency of two EXP groups passing through target area were still significantly less than that of two CONT groups after exercises training(P<0.05),and there Was no significant difference between EXP1 and EXP2 groups.③In the immanohistochemical staining of GluR2,the gray values in hipocampus of two convulsion groups were significantly lower than that of two control groups(P<0.05). Conclusion:Penicillin induced recurrent convulsion could cause long-term effects on learning and memory in rats,which may be associated with down-regulated GluR2 expression in hippocampus.Physical exercises could improve learning capacity,of convulsion rats but not memory capacity.

Objective: To explore the therapeutic efficacy and mechanism of transcutaneous electrical acupoint stimulation (TEAS) for menopausal insomnia. Methods: A total of 80 patients with menopausal insomnia were randomly divided into a control group and an observation group, with 40 cases in each group. The patients in the control group received conventional Western medication treatment, and the patients in the observation group received TEAS on the basis of conventional Western medication treatment. The treatment for both groups lasted for 4 weeks. Before and after treatment, Pittsburgh sleep quality index (PSQI) and modified Kupperman scale were evaluated, and the serum levels of estradiol (E2) and follicular stimulating hormone (FSH) were measured. The therapeutic efficacy was evaluated after treatment. Results: After treatment, the total effective rate of the observation group was significantly higher than that of the control group (P<0.05); in the control group, the improvement of PSQI score was significant (P<0.05), while the change of modified Kupperman score was insignificant (P>0.05); the PSQI and Kupperman scores in the observation group were significantly improved after treatment (both P<0.05), and there were significant differences between the observation group and the control group in PSQI and Kupperman scores (both P<0.05). After treatment, the serum E2 and FSH levels in the control group were not statistically different from those before treatment (both P>0.05); the serum E2 level was significantly increased (P<0.05), and the FSH level was decreased (P<0.05) in the observation group after treatment, and the between- group differences in serum levels of E2 and FSH were significant (both P<0.05).Conclusion: TEAS plus conventional Western medication in treating menopausal insomnia is effective, and can significantly improve the symptoms of insomnia and menopause, which may be related to the regulation of serum E2 and FSH levels.

Objective To investigate the effects of different concentrations of sevoflurane on proliferation and invasion in human prostate cancer PC3 cell line. Methods The cells were randomly divided into four groups:control group and three sevoflurane groups (exposed to 1.7%, 3.4%and 5.1%sevollurane for 2, 4 and 6 h respectively). The ability of prolifera-tion and invasion of PC3 cells were evaluated by MTT and Transwell invasion assays. Western blot analysis was performed to detect the protein expression of hypoxia inducing factor 1 alpha (HIF-1α) in PC3 cells. Results The ability of proliferation and invasion of PC3 cells was significantly increased after being exposed to sevollurane for 2, 4 and 6 h (P<0.05), and the expression of HIF-1αprotein in PC3 cells was significantly higher after being exposed to sevollurane for 2,4 and 6 h (P<0.05), showing the Treat 3 group>Treat 2 group>Treat 1 group (P<0.05). Conclusion Sevoflurane can promote prolifer-ation and invasion in PC3 cells, which are gradually increased with time and concentration of sevoflurane treatment. The mechanism may involve in the up-regulation of HIF-1αexpression.

Objective To evaluate the polymorphism and forensic applicability of Y - DNA STR locus DYS391. Method 111 unrelated male samples from Guangzhou Han population were investigated.DYS391 primer was labeled with FAM. PCR products were detected using denatured PAGE with GenScan Software on the ABI Prism 377 DNA sequencer.Results Frequency data of 3 different DYS391 alleles were obtained. Conclusion The results demonstrated that the DYS391 locus is of high human specificity. No mutation was found.