BACKGROUND: Construction of lentivirus vector has the advantages of simplicity,it is regard as the most effective and successful method in transgene therapy. OBJECTIVE: To clone the metastasis suppressor gene KiSS-1 from human normal placenta tissue and construct its lentivirus vector. DESIGN,TIME AND SETTING: The open experiment was performed at the Laboratory of Fujian Normal University from September 2006 to December 2007. MATERIAL: The pNL-IRES2-EGFP vector was conservated by the Laboratory of Fujian Normal University. METHODS: Total RNA was extracted from human placenta tissue. The opening reading frame cDNA of KiSS-1 was isolated by using RT-PCR,and cloned into its lentiviral vector pNL-IRES2-EGFP to construct expression plasmid pNL-IRES2-EGFP-KiSS-1. MAIN OUTCOME MEASURES: Clone objective gene fragment of KiSS-1,restriction enzyme digestion and gene sequencing of the recombinant plasmid pNL-IRES2-EGFP-KiSS-1 were observed in the study. RESULTS: The nucleotide sequence isolated from the recombinant plasmid pNL-IRES2-EGFP-KiSS-1 was confirmed the same as expected by restriction enzyme digestion and gene sequencing. CONCLUSION: The recombinant plasmid pNL-IRES2-EGFP-KiSS-1 has been constructed successfully.

Objective To research the tissue structure changes of vessels wall in the patients with liver cirrhotic portal hypertension and to investigate the role of inducible nitric oxide synthase (iNOS) in development process of liver cirrhosis.Methods The left portal vein,left hepatic artery and left hepatic vein (n=27) were collected from the patients with liver transplantation as the case group and the corresponding vessels of traumatic hepatectomy were collected as control group (n=15).HE staining and Masson staining were performed respectively.The hepatic arteries were stained with elastic fibers and immunized with anti-human iNOS polyclonal antibody.Results Compared with the control group,the portal vein intima and tunica media in the case group were thickened,the smooth muscle cells of tunica media were hyperplasia and hypertrophy,partial smooth muscle cells protruded into the intima;the irregular proliferation of hepatic arterial intima,arterial luminal stenosis,internal elastic membrane flatten,and even fracture,the tunica media smooth muscle hyperplasia to the intima were also observed;no obvious structural changes were observed in the hepatic vein wall.The higher expression of iNOS was found in the cytoplasm of hepatic artery smooth muscle cells (P<0.01).Conclusion The walls of liver artery and portal vein in the patients with liver cirrhosis would secondarily develop the tissue structure reconstruction,which is the results of human body′s own compensatory mechanism,the higher expression of iNOS accelerats the occurrence and development of the tissue structure reconstruction.

Patients who have received coronary artery interventional therapy exhibit visible anxiety and depression, which is closely related to prognosis and life quality. Therefore, for coronary artery stent patients,exception of anticoagulation and antihyperlipedemic drugs, psychological support, cognition therapy and distorted behavior rectification should be applied. The medication of anti-anxiety or depression is of great importance in ameliorate mental state, relieve mental load, as well as enhance prognosis of patients.

A large number of epidemiological studies have indicated that periodontal disease and ischemic stroke have a certain correlation, which may be its independent risk factor, but the exact mechanism is not fully understood. This article reviews the epidemiological studies of periodontal disease and ischemic stroke as well as its possible mechanism of action.

Objective To understand the effect of environment variables on anxiety in the soldiers at high altitude.Methods A total of 267 soldiers at high altitude were investigated with the self-rating anxiety scale,the environmental scale and Eysenck personality questionnaire.Results Compared with norm(29.78±0.46),the soldiers at high altitude had higher sore of anxiety(37.22±6.64;t=17.92,P＜0.01).One-way ANOVA and independent.samples t test showed that the anxiety(38.37±5.93,36.65±6.951,36.75±6.88)of military people at high altitude had not evidently difference in different altitude(≥3587.5m,3587.5～2767.9m,≤2767.9m).Parental culture.length of military service(F=3.25,P＜0.05)and neuroticism(R=0.52,P＜0.01)had evidently effect on the depression of soldiers.Conclusion The occurrence of anxiety in the soldiers at high altitude has a higher level.Parental culture.1ength of military service and neuroticism had predictive value for depression.

Purpose To evaluate ultrasonography in diagnosis of high recurrence risk gastrointestinal stromal tumors (GIST) by retrospective studying the ultrasound (US) imaging features of GITS. Materials and Methods US features of 72 GIST cases confirmed histopathologically were retrospectively studied. The tumor size, shape, boundary, echogenicity and blood flow of GITS were observed and compared with the results of histopathology. Results Of all the 72 tumors, 34 were originated in the stomach, 14 in the intestine, 2 in the colon, 8 in the rectum, 9 in the mesentery, 1 in the posterior peritoneum, 1 in the omentum and 3 huge masses of non definite origin. There were 7 cases with very low recurrence risk, 14 cases with low risk, 11 cases with intermediate risk and 40 cases with high risk. High recurrence risk group showed no statistical difference in heterogeneous hypoechoes and blood flow (P>0.05), but showed significantly higher rates in following US features:size larger than 5.0 cm, irregular shape, obscure boundary and cyst-solid echogenecity (P<0.001), which showed the sensitivity of 95.0% (32/40), 72.5% (29/40), 25.0% (10/40), and 55.0% (22/40), respectively; and the specificity of 59.4% (19/32), 81.3% (26/32), 90.6% (29/32), and 87.5% (28/32), respectively. Conclusion US can serve as a mean of screening GIST. The recurrence risk can be evaluated based on the tumor size, shape, boundary and echogenecity to guide clinical decisions and prognosis evaluation.

Objective:To design a new rehabilitation system for cerebral apoplexy patients, and help patients complete rehabilitation treatment on the bed.Methods: Design the basic structure of the system through mechanical technology, make the realization of the function by using circuit and wireless control system, global-eye system was embedded in it by using the principle of the 3G to 4G intelligent technology for improving the humanized design of the system. Results:Through application in physical therapy, nursing, clinical rehabilitation, and clinical therapy in hospitals, it achieved good economic and social benefits, got the recognition of patients, clinicians and rehabilitation therapists. Conclusion: It can be used for the treatment of cerebral apoplexy, reduce medical expenditure, save amount of social resource and improve the work efficiency of medical staff.

Medicine, Chinese Traditional

Medicine, Chinese Traditional

AIM:To analyze the regulatory effect of Yigu capsule on core binding factor alpha 1 (cbf ?-1) gene expression in bone of ovariectomized osteoporosis (OP) rats. METHODS: Thirty-six 10-month old Sprague-Dawley female rats were randomized into three groups: sham-operated group, model group and drug group. After intervention by the corresponding methods, the femurs were collected, SYBR green Ⅰ fluorescence quantitative PCR technique was applied with the internal control of GAPDH according to the relative quantitative formula (2-Ct), the differentially expressed multiples between the model group or drug group and sham-operated group were calculated. RESULTS: Quantitative formula analysis showed that the level of cbf ?-1 gene expression in bone of model groups was decreased than that in sham-operated rats [compared with sham-operated group, P0.05). CONCLUSION: The results of this study show that cbf ?-1 gene expression in bone tissue of ovariectomized OP rat is decreased, and Yigu capsule increases the level of cbf ?-1 gene expression in bone of OP, indicating that Yigu capsule induces bone marrow mesenchymal stem cells into osteoblasts.