Mitochondrial calcium homeostasis plays a key role in maintaining various cellular characteristics and mediating cellular physiological function and pathological processes .Although it has long been known that mitochondria takes up Ca2+, the molecular identities of the channels and transporters involved in this process are revealed only recently . Here, we review the structure and function of the channel-forming subunit, mitochondrial calcium uniporter (MCU) and its regulators, which include MICU1, MICU2, and MCUR1.

Objective To understand clinical specimen hemolysis Staphylococcus (SHA)distribution characteristics and re-sistance and sensitivity to 20 kinds of antibiotics,reasonable to guide the clinical treatment of SHA infection.Methods Rou-tinely cultured and isolated bacteria.Used the United States BD Phoenix-100 automated microbial identification and suscepti-bility instrument to identify bacteria and susceptibility testing,and susceptibility testing all used the instrument broth dilu-tion method,according to the USA CLSI2015 [1]regulations standards.Results 162 strains of SHA from the distribution of age,children under the age was one of the highest (30.9%),and from the distribution department,mainly distributed in ped-iatrics (30.9%),department of critical care medicine (22.2%),medicine (17.3%),surgery (12.3%).From the specimen type distribution,were mainly distributed in the blood (33.3%),sputum (25.9%),wound (11.1%) and discharge (9.9%).In the 162 strains of SHA,the proportion of the MRSH was 93.8%,of which 152 strains of MRSH incidence of multiple drug resistance (MDR)was as high as 61.8%.Compared with MSSH,antibiotic resistance rate of MRSH was sig-nificantly higher.The resistance rate of MRSH to ampicillin,cefoxitin,penicillin G,erythromycin was extremely high,more than 98.7% the former of cefoxitin,penicillin G,ampicillin,erythromycin resistance was extremely high,more than 98.7%. The sensitive rate of both to rina thiazole amine,vancomycin,amikacin was 100%,and the rate to Fusidic acid,teicoplanin, nitrofurantoin was also high,more than 9 5.5%.Conclusion Linezolid,Vancomycin,Amikacin,Fusidic acid,Teicoplanin and Nitrofurantoin because all can be used as empiricaluse of SHA infection,other antibiotics chooses to in addition to drug sen-sitivity tests results.

Dry age-related macular degeneration(AMD)is a prevalent form of irreversible blindness worldwide in the elderly population with an increasing incidence in China.It is found that some dry AMD will develop to wet AMD and therefore damage visual function.Pathogenesis of AMD is below understand now,but it is thought to be related to the light-induced ocular damage,oxidative stress response,extracellular disposition and immunology.Up to now,effective and safe prevention and treatment methods of AMD are still studying,especially wet AMD,but the concern on dry AMD is not enough.Some basic and clinical pharmaceutics researches on AMD include antioxidants,anti-extracellular disposition drugs and anti-inflammatory agents.With the rise of aging population,we should pay special attention to the research on pathogenesis,diagnosis and treatment of dry AMD.

Objective To evaluate the clinical value of infusing dehydrated alcohol on pancreatic pseudocyst (PPC). Methods Twenty one patients with D'Egidio type I PPC underwent CT or ultrasound (US) guided percutaneous catheter drainage, and CT or US were used to re-evaluate the efficacy of drainage. If no residual cavity was observed, the drainage tube was clamped for 3~7 d, and then the tube would be pulled out. Non-curative cases with catheter drainage for 21 days were given dehydrated alcohol 20 ml infusion. Results The curative rate by using percutaneous catheter drainage was 71.43% (15/21). Among the remaining 6 patients, the treatment was effective in 3 patients and ineffective in other 3 patients. 2 cases of non-infective mutli-antrum pancreatic pseudocyst, 1 case of infective single antrum and 3 cases of infective multi-antrum were included in the 6 non-curative cases, and all of them recovered completely 6~21 d after dehydrated alcohol infusion. No complication and recurrence were found during the 6~12 follow-up. Conclusions Percutaneous puncture and dehydrated alcohol infusion for infective or mufti-antrum pancreatic pseudocyst was effective and safe.

Objective:To explore features and relevant influencing factor of ecological executive function in children with childhood absence epilepsy (CAE).Methods:Forty children with CAE (CAE group) and 40 healthy children with physical examination (control group) from April 2017 to July 2020 in Anhui Provincial Children′s Hospital were selected. The behavior rating inventory of executive function (BRIEF) parental questionnaire was used to evaluate the executive function of children. The differences of ecological executive function between 2 groups were compared.Results:The BRIEF total score, behavioral regulation index (BRI) and metacognition index (MI) in CAE group were significantly higher than those in control group: (52.03 ± 10.89) scores vs. (44.05±5.06) scores, (49.45 ± 9.93) scores vs. (43.85 ± 4.70) scores and (53.18 ± 11.24) scores vs. (44.95 ± 5.32) scores, and there were statistical differences ( P<0.01). Multiple stepwise regression analysis result showed that inhibit inhibition, shift, emotional control, initiation, working memory, planning, organization, monitoring, MI and total score were correlated with disease control ( P<0.01), and disease control had a negative predictive effect on them ( R2 = 0.174, 0.158, 0.234, 0.325, 0.383, 0.337, 0.378, 0.199, 0.463 and 0.435); BRI was correlated with onset age and disease control ( P<0.01 or <0.05), and onset age and disease control had a negative predictive effect on BRI ( R2 = 0.336). Conclusions:Children with CAE have ecological executive dysfunction. The control of the disease and the onset age are the main factors affecting the ecological executive function.

AIM: To know the psychological conditions of various cataract patients in pre- and post-operative periods.METHODS: From June 2008 to June 2009, 278 cataract patients had been asked to complete a questionnaire anonymously of their self-evaluation of symptoms scale (SCL) before and after operation. In their pre-operative and post-operative surveys, the results were compared with the norms respectively. Patients were divided into two groups. Group A were age-related cataract patients; while Group B were cataract patients with glaucoma, trauma or metabolic disease.RESULTS: When cataract patients' masculine scores of somatization, depression, anxiety and fear factors before and after the operation rank higher than the norms, the differences had statistical significance(P＜0.05), especially the somatization factor(P＜0.01).While cataract patients with diseases such as glaucoma, trauma or diabetes got higher marks than age-related cataract patients in the aspects of anxiety, somatization, depression (P<0.05), and fear factors, the differences were of statistical significance(P<0.01).CONCLUSION: Cataract patients both in pre- and post- and operative periods have somatization behaviors and emotions of depression, anxiety and fear. Cataract patients with glaucoma, trauma or diabetes especially anxiety have more obvious symptoms than age-related cataract patients.

Objective To observe and evaluate the effect of ultrasound guided percutaneous ethanol injection for hyperparathyroidism secondary to chronic renal failure. Methods Fifty-three patients with secondary hyperparathyroidism in Nanjing General Hospital Military Command from January 2011 to May 2013 were treated by percutaneous ethanol injection. The echogenicity, size and blood supply of parathyroid glands were observed by color Doppler ultrasound before and after treatment. Parathyroid hormone (PTH) and volumes of parathyroid glands were analyzed and compared with non-paired t test. Results Before treatment, the parathyroid gland volume was (5.28±0.84) cm3. The enlarged parathyroid glands had homogeneous hypoechogenicity and abundant blood supply, and the PTH was (1041.6±37.1) ng/L. After treatment, the size of solitary parathyroid gland was (3.93±0.67) cm3 which did not change significantly compared with that before treatment. The echogenicity of parathyroid glands enhanced and blood supply decreased after injection ethanol into solitary parathyroid. PTH [(509.2±27.6) ng/L] decreased obviously (t=3.792, P < 0.05). Conclusion Color Doppler ultrasound guided percutaneous injection of ethanol has an important role in the treating of secondary hyperparathyroidism and the curative effect can be observed timely.

Vitreo-retinal surgery is one of the effective methods in treating severe vitreoretinopathy.However,there remains great variance in surgical quality,outcomes and success rates among hospitals of different areas in China.This article focuses on how to im- prove in surgical outcomes and success rate as well as to avoid complications of vitreo-retinal surgery.Specific issues are addressed on such as indications and timing of vitreo-retinal surgery,management of pre-operative patients,applications of new appliances and mate- rials,maneuver of surgical techniques and nurturing appropriate habits of surgery.

Objective To investigate the possibility of pulp regeneration of different irreversible pulpitis with absorbable gelatin sponge as the scaffold,and dental seed cells,induced molecules and MTA closed.Methods 150 patients with irreducible pulpitis,who were removed the crown infection pulp and retained the root living pulp,according to the visiting sequence,were randomly divided into two groups.The experimental group was given absorbable gelatin sponge as the scaffold and MTA closed.The control group was given calcium hydroxide coverage.The symptoms situation and pulp situation were observed.Results 6 months after surgery,the success rate of acute pulpitis and chronic closed pulpitis in the experimental group was 76.5％ and 47.0％,which were significantly higher than those of the control group (14.3 ％,8.8％) (P ＜ 0.01).In the experimental group,the success rate of chronic hyperplastic pulpitis and chronic ulcerative pulpitis was 33.0％ and 35.0％,which were higher than those of the control group(9.1％,6.0％),but the differences between the two groups were not statistically significant (P ＞ 0.05).Conclusion Pulp regeneration in vivo is possible with absorbable gelatin sponge as the scaffold and MTA,pulp regeneration of acute pulpitis and chronic closed pulpitis are more likely to induce.

Traditional Chinese medicine owns the duality of commodity property and quasi-public goods in the process of production and circulation. According to the duality of traditional Chinese medicine, giving full play to government function is the key to guaranteeing and promoting the quality and security control in the dryness process of traditional Chinese medicine. Government functions mainly focus on improving the quality and security control ability of production operators and stimulating quality and security control motive of producers. This article began with the duality of traditional Chinese medicine, expounded the government functions in the dryness process and quality control of traditional Chinese medicine from macroscopic and micro levels, and proposed the measures of improving dryness process based on government functions.