Objective To cultivate human retinal capillary endothelial cells (HRECs) and establish two-dimensional model of human retinal vessels in vitro. Methods In a fibronectin-coated raising pound, HRECs were cultured by non-serum human-endothelial-cells substrate and two-dimensional model of human retinal vessels was established. Horseradish peroxidase was used to detect the permeability. Some of the vascular models were cultivated with 5 ng/ml vascular endothelial growth factor (VEGF), whose changes of permeability was compared with which of the models without cultivation with VEGF. The effect of VEGF on vascular permeability was observed. Results Meshy vascular structure came into being due to the confluent HRECs after 2 to 4 days. Comparatively complete two-dimensional vascular model after about 6 days. VEGF increased vascular permeability and promoted the formation of blood vessels. Conclusion HRECs can be cultivated successfully with human-endothelial-cells substrate; standard retinal two-dimensional vascular model in vitro can be established by using cellular raising pound and non-serum human-endothelial-cells substrate.

Objective To study the efficacy and toxicity of tetrakis(hydroxymethyl)phosphonium sulfate(THPS)in indoor air disinfection.Methods The orthogonal experiments were employed with three factors including concentration of THPS,temperature and time,and four levels including the concentrations of THPS,0.05%,0.1%,0.5%,1%,and the temperature,15℃,20℃,25℃,30℃,and the time,2 h,4 h,8 h,16 h.The germicidal effect of THPS with those factors was assessed.Using modified method,Daphnia magna straus(Cladocera crustacea),Brachydanio rerio,mice were randomly divided into 5 dose groups and a control respectively.For Daphnia magna straus(Cladocera crustacea),123.03 mg/L,134.90 mg/L,147.91 mg/L,162.18 mg/L and 177.83 mg/L,for Brachydanio rerio,42.66 mg/L,57.54 mg/L,77.62 mg/L,104.71 mg/L and 140.90 mg/L,for mice,183.60 mg/kg,293.75 mg/kg,470.00 mg/kg,752.00 mg/kg and 1 203.20 mg/kg.The data for 2th,4th and 7th day of treatment were collected for calculation of the 48h-EC50,the 96h-LC50 and the acute oral LD50 in Daphnia magna straus(Cladocera crustacea),Brachydanio rerio and mice.Results The maximal germicidal rate of THPS in indoor air was over 86%.At the concentration of 0.5% THPS,4 h reaction time,germicidal efficacy of THPS was optimal.The toxicity test indicated that the acute oral LD50 for mice was 492.72 mg/kg,the 48 h-EC50 for Daphnia magna straus(Cladocera crustacea)was 143.61 mg/L,the 96h-LC50 for Brachydanio rerio was 84.17mg/L.Conclusion The toxicity of THPS is low and can be used for indoor air disinfection with a good efficacy and the potential benefit for environment.

OBJECTIVE:To establish a HPLC method for the determination of 5-fluorouracil in human plasma METHODS:The plasma samples were extracted with ethyl acetate-isopropanol(85∶15,V/V)following precipitation of plasma proteins with ammonium sulfate The solvent was evaporated to dryness under a stream of nitrogen The dry extract was dissolved in mobile phase and then injected into the Diamonsil C18 column(150mm?4 6mm,5?m)and 0 01mol/L KH2 PO4(pH5 5) was taken as mobile phase The flow rate was 1 5ml/min,UV detection wavelength was 267nm The internal standard was 5-bromouracil RESULTS:The minimal detectable drug concentration in plasma was 0 025?g/ml The calibration curve was linear over a range from 0 3?g/ml to 10?g/ml The intra-day RSD≤5 0%(n=5) and the inter-day RSD≤11 5%(n=5) CONCLUSION:The method can be used for studying the pharmacokinetics and bioavailability of 5-fluorouracil

Acupuncture Points ; Acupuncture Therapy ; Adolescent ; Adult ; Aged ; Facial Paralysis ; therapy ; Female ; Humans ; Male ; Middle Aged ; Young Adult

Animals ; Electric Stimulation ; Neurons ; pathology ; physiology ; Pain Threshold ; Rats ; Rats, Sprague-Dawley ; Red Nucleus ; pathology ; physiology

Objective To evaluate the efficacy of different doses of dexmedetomidine mixed with ropivacaine for brachial plexus block.Methods One hundred and twenty ASA physical status Ⅰ or Ⅱ patients of both sexes,aged 20-60 yr,weighing 40-70 kg,scheduled for upper extremity surgery under brachial plexus block,were randomly divided into 6 groups (n =20 each):ropivacaine group (group R) and different doses of dexmedetomidine groups (groups RD1-5).Brachial plexus block was performed using the interscalene approach.In group R,interscalene brachial plexus block was performed using 0.5 ％ ropivacaine 25 ml (single injection).In groups RD1.5,interscalene brachial plexus block was induced with a mixture (25 ml) of dexmedetomidine 0.25,0.50,0.75,1.00,1.25μg/kg and 0.5 ％ ropivacaine,respectively.The onset time and duration of sensory and motor block were recorded.The adverse effects such as adverse cardiovascular events,excessive sedation,and pneumothorax were also recorded.Results Compared with group R,the onset time was significantly shortened,and the duration of block was prolonged (P ＜ 0.05).Compared with groups RD1 and RD2,the onset time was significantly shortened,and the duration of block was prolonged in groups RD3 5 (P ＜ 0.05).There was no significant difference in the parameters mentioned above between groups RD1 and RD2 or among groups RD3,RD4 and RD5 (P ＞ 0.05).Some patients developed bradycardia,hypotension or excessive sedation in groups RD4 and RD5,while no adverse effects were observed in the other groups.Conclusion Dexmedetomidine 0.75 μg/kg mixed with 0.5 ％ ropivacaine 25 ml can be safely and effectively used for brachial plexus block in patients.

Objective:To develop an HPLC-DAD method for the simultaneous determination of seven active constituents chloro-genic acid, 3,5-dicaffeoylquinic acid,linarin, luteolin-7-O-β-D-glucoside, apigenin-7-O-β-D-glucoside , luteolin and acacetin in the different parts of Dandrantherna morifolium( Ramat. ) Tzvel. Methods:The chromatographic separation was performed on a Diamonsil C18 column (200 mm × 4. 6 mm,5 μm) with 0. 2% phosphoric acid-acetonitrile as the mobile phase with gradient elution at the flow rate of 1 ml·min-1 . The detection wavelength was set at 348 nm,and the column temperature was maintained at 30 ℃. Results:Chlorogenic acid, 3,5-dicaffeoylquinic acid, linarin, luteolin-7-O-β-D-glucoside, apigenin-7-O-β-D-glucoside , luteolin and acacetin was linear within the range of 0.014-0.560 μg(r =0.999 5) ,0.031-1.260 μg(r =0.999 6), 0.022-0.900 μg(r =0.999 8), 0. 036-1. 440 μg(r=0. 999 6),0. 029-1. 160 μg (r=0. 999 7) , 0. 005 5-0. 220 0 μg (r=0. 999 8) and 0. 006 5-0. 260 0 μg (r=0. 999 9), respectively. The average recovery was 97. 91%, 97. 91%, 97. 55%, 96. 43%, 96. 44%, 97. 07% and 97. 48%, and RSDs was 1. 24%, 1. 29%, 1. 48%, 1. 83%, 0. 67%, 1. 88% and 1. 80%, respectively. Conclusion: The method is convenient, accurate and reproducible in the quality control of the multicomponents in different parts of Dandrantherna morifolium( Ramat. ) Tzvel.

Objective To investigate the cooperative antifungal effect of antibacterial peptide MUC7 combined with Bifidobacte‐ria in vitro .Methods The antifungal effect was observed and measured by viable count and the Oxford cup method .Results Two methods exhibited more potent antifungal effect on Candida albicans ,Candida albicans ,Candida krusei and Candida parapsilosis in MUC7 combined with Bifidobacteria group .The colonies′numbers in MUC7 combined with Bifidobacteria group were 2 .00 ± 1 .13 , 2 .00 ± 1 .42 ,5 .00 ± 2 .03 ,2 .00 ± 1 .39 respectively by viable counting ,which was lower than thoes in the saline group and Bifidobacterium group (P<0 .01) ,these two groups were significant lower than those in MUC7 group (P<0 .05);the inhibition zone in MUC7 combined with Bifidobacteria group were (29 .00 ± 2 .17) ,(31 .00 ± 3 .25) ,(29 .00 ± 2 .89) ,(30 .00 ± 3 .36)mm de‐tected by the Oxford cup method ,which showed a significantly difference with the saline group ,Bifidobacterium group and MUC7 group(P< 0 .05) .Conclusion Antibacterial peptide MUC7 combined with Bifidobacterium exhibits good antifungal effect which may provide a foundation for the further research on a new generation of antifungal Bifidobacterium preparation .

Objective To investigate the level of depression in patients with systemic lupus erythematosus (SLE).Methods Fifty six in patients and out patients with SLE were investigated with questionnaire of self rating depressive scale (SDS).Results Of these patients 41% had clinical features of depression.Tiredness,hebetude,hyposexuality,despair,irritability,depression,thinking difficulty,insommia,uneasiness and vacancy were more common.Most of these patients are manual worker,poorly educated ( P 0 05).Conclusion Patients with SLE have symptoms of depression to a certain extent.Doctors should pay close attention to their mentality and emotion in diagnosis and treatment of the prtients with SLE.

AIM To study the thrombolytic effect of non hemorrhagic fibrinolytic enzyme from Agkistrodon acutus venom on animal thrombosis. METHODS By means of pulmonary embolism,arterial thrombosis model in rabbits and venous thrombosis model in rats. The non hemorrhagic fibrinolytic enzyme from Agkistrodon acutus was given by intravenous injection at 0 14, 0 7, 1.4 mg?kg -1 three different doses respectively. Normal saline was used as negative control and thrombolytic effects was observed. RESULTS It was showed that moist weights of arterial thrombosis, venous thrombosis and pulmonary embolism were markedly lightened and has statistical significance compared with normal saline ( P