1.Advances on Polyketide Biosynthesise by Microorganism and the Diversity of Polyketide Synthases
Li-Cheng REN ; Shi-Xiang BAO ;
Microbiology 1992;0(01):-
Polyketides are very large group of natural products with functional and structural diversity.Most of them are produced by microor- ganism and have medicinal activities,including antibiotic,anticancer,antifungal and antiparasitic properties.The researchs in this area have progressed greatly.More and more polyketides are discovered,on the other hand the mechanisms of biosynthesis of those various polyketides are researched more deeply and clearly.The article reviewed the progress of the research in the diversity of polyketide synthases and the mechanisms of polyketide biosynthesise.
4.Professor Dong Gui-rong's experiences in treatment of child cerebral palsy.
Guan-nan YANG ; Cheng-jia LI ; Chun-ling BAO
Chinese Acupuncture & Moxibustion 2014;34(9):899-901
According to the characteristics of the etiology and pathogenesis of child cerebral palsy, on the basis of "regulating the mind in treatment of all kinds of diseases" and "regulating the functions of five zang organs with back-shu points", Professor DONG Gui-rong applied the penetrating needling technique on the scalp points and acupuncture at back-shu points of five zang organs in the treatment of child cerebral palsy. The valuable clinical experiences have been summarized as "regulating the mind with scalp needling technique, benefiting the brain and opening the orifice", "regulating five zang organs with back-shu points" and "integration of acupuncture and rehabilitation, and function reconstruction". Two effective cases were introduced.
Acupuncture Points
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Acupuncture Therapy
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Cerebral Palsy
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therapy
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Child, Preschool
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Humans
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Male
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Treatment Outcome
5.Effects of Ang-Ⅱ on c-fos mRNA and pro-α1ⅠmRNA of cadiac FBC in rat embryo
Yi, JIN ; Bao-yu, LI ; Cheng-hao, GUO
Chinese Journal of Endemiology 2001;20(3):169-171
Objective To observe the effects of Ang-Ⅱ on expressions of c-fos mRNA and pro-α1[Ⅰ]mRNA in fibroblasts (FBC) of rat embryo in the course of collagen synthesis.Methods The change of free Ca2+([Ca2+]i) was tested in fluorometry and the expressions of c-fos mRNA and pro-α1[Ⅰ]mRNA were tested by RT-PCR method in incubated FBC.Results The level of[Ca2+]i in FBC and the expressions of c-fos mRNA and pro-α1[Ⅰ]mRNA were enhanced with the increase of Ang-Ⅱ concentration(10-8~10-5and 10-7~10-6mol/L).Conclusions The expressions of c-fos mRNA and pro-α1[Ⅰ]mRNA were induced by Ang-Ⅱ activating signal system in FBC.
6.Research progress of injectable bone tissue engineering scaffold material
Yamei LI ; Juan AI ; Fei BAO ; Cheng LIN
International Journal of Biomedical Engineering 2017;40(2):133-136,141
Injectable bone tissue engineering scaffold material has a certain form and mechanical strength.It can be loaded with seed cells for injection to the defected site of bone tissue in the form of fluid.After that,structural recovery and functional reconstruction are achieved,and the new bone is formed ultimately.This material has the characteristics of small trauma and good plasticity.It can repair the irregular bone defects and well associate with growth factor,which is the ideal method for repairing bone tissue defects up to now.Among the various injectable bone tissue engineering materials,bioceramic materials,polymer materials,etc.have been proved to have a high biological compatibility and good mechanical properties.That makes these materials become the focus of current research in bone tissue engineering materials.In this paper,the development and application of bioceramic materials,polymer materials,bioceramics and polymer composites were reviewed.
7.Comparison of131I-whole Body Scan and18F-FDG PET/CT on the Metastatic Management of Differentiated Thyroid Cancer After Operation
Yongjun LI ; Zhaoqiang XU ; Wei LIU ; Xu CHENG ; Lihua BAO
Chinese Journal of Medical Imaging 2014;(11):805-810
Purpose To explore the clinical value of131I-whole body scan (131I-WBS) and 18F-FDG PET/CT on the metastatic management of patients with differentiated thyroid cancer (DTC) after operation.Materials and Methods Serum thyroglobulin (Tg) of 27 patients after DTC surgery was measured one day before131I therapy, and the patients underwent131I-WBS on 3-5 days after131I therapy. According to the results of Tg and 131I-WBS, all the patients were divided into 4 types as follows: type I: Tg (+),131I-WBS (-); type II: Tg (+),131I-WBS (+); type III: Tg (-),131I-WBS (+); and type IV: Tg (-), 131I-WBS (-). Then the patients received18F-FDG PET/CT scan within a week after 131I-WBS. The results of131I-WBS and18F-FDG PET/CT scan were analyzed according to histopathologic findings or clinical and imaging follow-up of at least 6 months as diagnosis standard.Results The examinations uncovered that metastasis occurred in 52 lesions of 22 cases out of the 27 cases.131I-WBS revealed 24 metastatic lesions (46.2%) in 8 cases (36.4%), and18F-FDG PET/CT showed 35 metastatic lesions (67.3%) in 15 cases (68.2%), the difference with statistic significance (χ2=4.46 and 4.74,P<0.05). Metastasis were confirmed in 13 type I cases (100.0%); 5 type II cases (100.0%); 3 type III cases (100.0%) and 1 type IV case (16.7%), respectively. The rate of detecting metastasis by 18F-FDG PET/CT was 100.0% in type I, 20.0% in type II, 0% in type III respectively, the difference with statistic significance (χ2=9.14 and 10.11,P<0.05); and 100.0% in type IV, the difference without statistic significance (χ2=1.25,P>0.05). Based on that, therapeutic plans were modified for 13 patients with type I metastasis and 1 patient with type IV metastasis.Conclusion131I-WBS and18F-FDG PET/CT are complementary for detecting metastatic lesion and help to decide the following treatment of DTC patients after surgery. Moreover,18F-FDG PET/CT shows advantages in detecting metastatic lesion in Tg-positive and negative131I-WBS type of DTC patients after surgery, thus can provide an important clinical guidance for this type of patients.
8.Electrophysiologic study of f-wave amplitude in chronic atrial fibrillation associated with rheumatic heart disease
Li LI ; Bao-Cheng JIA ; Bao-Ren ZHANG ; Zeng-Wei WANG ; Jia-Lin ZHU
Academic Journal of Second Military Medical University 2005;26(2):127-130
To investigate the electrophysiologic characteristics of f-wave amplitude and to evaluate its role in development and persistence of chronic atrial fibrillation (AF) associated with rheumatic heart disease (RHD). Methods: Epicardial mapping was performed in 44 patients with chronic AF of RHD who underwent heart valve surgery. Ten patients with supraventricular tachycardia served as the control group. Results:The f-wave amplitude of left atrium (LA) and middle and low LA posterior regions were significantly lower than those of the control group. The f-wave amplitudes of the upper, middle and low sections in LA posterior region were significantly lower than those in right atrium (RA) (P<0.05). The f-wave amplitudes were compared before and after electrocardioversion in 14 patients with chronic AF. The mean atrial electrogram amplitude during sinus rhythm was significantly higher than that during AF (P<0.01).The f-wave amplitude in left appendage was higher than that in LA posterior region (the upper,middle and the lower part),P<0.05.The f-wave amplitude in the upper section of LA was significantly higher than that in the middle section. The f-wave amplitude in AF group was not correlated to the diameter or volume of both atria. Conclusion: There are amplitudes differences between the upper, middle and lower LA,suggesting that the middle and lower sections of LA posterior wall may be the region producing anisotropy and reentrant circle.
9.C21 steroids from roots of Cynanchum otophyllum.
Xiang LI ; Mi ZHANG ; Cheng XIANG ; Yi QIN ; Jing HE ; Bao-Cai LI ; Peng LI
China Journal of Chinese Materia Medica 2014;39(8):1450-1456
Eleven C21 steroids were isolated from chloroform extract of roots of Cynanchum otophyllumby silica gel, MCI, ODS columns, and semi-preparative HPLC. Their structures were determined by spectroscopic data analysis as otophylloside B(1), caudatin-3-O-beta-D-cymaropyranosyl-(1-->4)-beta-D-oleandropyranosyl-(1-->4)-beta-D-cymaropyranosyl-(1-->4)-beta-D-cymaropyranoside (2), caudatin-3-O-beta-D-oleandropyranosyl-(1-->4)-beta-D-oleandropyranosyl-(1-->4)-beta-D-cymaropyranosyl-(1-->4)-beta-D-cymaropyranoside (3), caudatin-3-O-beta-D-oleandropyranosyl-(1-->4)-beta-D-digitoxopyranosyl-(1-->4)-beta-D-cymaropyranoside (4), otophylloside O (5), gagamine-3-O-beta-D-oleandropyranosyl-(1-->4)-beta-D-cymaropyranosyl-(1-->4)-beta-D-cymaropyranoside (6), sinomarinoside B (7), mucronatosides C (8), wallicoside J (9), stephanoside H (10), and qinyangshengenin-3-O-beta-D-oleandropyranosyl-(1-->4)-beta-D-cymaropyranosyl-(1-->4)-beta-D-digitoxopyranoside (11). Among them, compounds 2-3, and 6-11 were separated from the roots of this plant for the first time.
Cynanchum
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chemistry
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Drugs, Chinese Herbal
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chemistry
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isolation & purification
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Magnetic Resonance Spectroscopy
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Molecular Structure
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Plant Roots
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chemistry
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Steroids
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chemistry
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isolation & purification
10.Effect of Grp78 on the activation and expression of ERK 1/2 in human hepatocellular carcinoma tissues
Rongjian SU ; Zhen LI ; Liufang CHENG ; Hongdan LI ; Jia WEI ; Cuifen BAO
Acta Anatomica Sinica 2009;40(6):928-932
Objective We examined the Grp78, ERK1/2 and phospho-ERK1/2 expressions in hepatocellular carcinoma(HCC) tissue samples in vitro, we interfered the expression of Grp78 in SMMC-7721 cells to explore whether Grp78 is involved in ERK1/2 signal pathway. Methods The Grp78, ERK1/2 and phospho-ERK1/2 expressions were detected by immunohistochemistry and confirmed by Western blotting in 47 HCC tissue samples. The Grp78 expression in SMMC-7721 cells was interfered by plasmid transfection and siRNA, ERK1/2 phosphorylation and expression were determined by Western blotting. Results The Grp78 expression was significantly correlated with ERK1/2 and phospho-ERK1/2 in HCC tissue samples. Overexpression of Grp78 promoted ERK1/2 phosphorylation in SMMC-7721 cells and the increased ERK1/2 phosphorylation was inhibited by Grp78 knockdown. Conclusion Grp78 is involved in the regulation of ERK1/2 signal pathway and might be a potential target for the comprehensive therapy of HCC.