PCR-DGGE(denaturing gradient gel electrophoresis) and clone library analysis combined with culture dependent isolation methods were used to analyze the population composition and its stability over different production batches of one commercial biofertilizer.DGGE fingerprinting showed that samples from the same production batch had 80%~100% similarity coefficient and those from different batches had 80%~88%,an indication that population composition of the biofertilizer was relative stable within the same batch and from batch to batch.Isolation followed with 16S rRNA clone library analysis showed that the microorganisms contained in the products were not in agreement with the product's label.Only Lactobacillus,one of the 6 microorganisms labeled in the products,was detected in the samples.Other bacteria detected with the two methods were Bacillus,Monascus,Brevibacillus,Psudomonas and Penicillium,which were not listed in the product's label.These results showed that molecular ecological methods may provide a new technology for quality control of biofertilizers.

BACKGROUND: Mathematics ability is one of the basic abilities in human intelligence and mathematics disability is a universal type in key period of school-age children to master the mathematical concepts, perform the abstract calculation and form comprehensive mathematics ability, which is lack of a special rating scale and reference norm.OBJECTIVE: To establish a suitable evaluated norm for rating scale of pupil's basic mathematical abilities.DESIGN: Cross-sectional study of multi-phase stratified cluster random sampling.SETTING: The experiment was presided by Tongji Medical College collaborating with the Centers for Disease Prevention and Control of Beijing,Hebei province, Jiangsu province, shangdong province, Lengshuijiang,Hainan province and Hainan province, Herbin Medical University, Anhui Medical University, Sun Yat-sen University of Medical Sciences, West China University of Medical Sciences, Guiyang Medical College, Lanzhou Medical College and other units.PARTICIPANTS: Totally 22 039 people were investigated in nationwide,including 14 693 students (7 377 urban ones and 7 316 ones, respectively), who were randomly selected from Grade One to Six of urban and rural general elementary schools.METHODS: Between June and September 2003, students from Grade One to Six of general elementary schools were sampled. According to the Fifth National Population Census in 2000 and based on sex, nation, area and city or count, the Chinese Rating Scale of Pupil's Mathematic Abilities was applied in group-test on mathematic abilities of the cluster-selected students with multi-phase-stratified sampling method.MAIN OUTCOME MEASURES: Calculative ability, logical thinking and spatial-visual abilities and so on.RESULTS: The norm samples of 14 693 subjects including 7 377 urban students and 7 316 rural students all entered the result analysis. ①The mean scores of each sub-test for the two sampled subjects gradiently increased along with grades rising; the scores of subtests among various ages from urban and rural samples (paired comparison) had significant differences (P ＜ 0.05); the scores of sub-tests of urban samples in various grades were higher than those of rural samples (t=2.234-11.766, P ＜ 0.05). The urban (n=7 377) and rural (n=7 316) norms were established, respectively.②The reference norm was composed of converted T score and percentile P,the T scores in every mid-term and final-term were estimated to calculate Z scores; finally, the probability function P under normal distribution was achieved. ③The evaluation of mathematics abilities were classified into 5 grades: it was average with the scores of (-x)±s (P=16%-84%); good if between (-x)+s and (-x)+2s (P=84%-97.5%); excellent if exceeded (-x)+2s(P ＞ 97.5%); inferior if between (-x)-s and (-x)-2s(P=16%-2.5%); worse if lower than (-x)-2s (P ＜ 2.5%).CONCLUSION: The constituent ratio of normal samples is basically consistent with the data of total population and the distribution of samples in various grades and sexes is nearly equilibrate; there are significant differences in scores of most subtests on mathematics abilities between urban and rural children, which suggest that it is necessary to establish urban and rural norm, respectively.

OBJECTIVE:To observe the safety and immunological effects of Measles-mumps-rubella(MMR)attenuated live at-tenuated vaccine in children. METHODS:300 children aged 8-12 months receiving inoculation were selected from Changsha Hospi-tal for Maternal and Child Health Care during Jan. 2015-Apr. 2016 to observe safety and immunological effects. Those children were divided into MMR group,measles vaccine group,mumps vaccine group and rubella vaccine group according to vaccine type, with 75 cases in each group. The occurrence of ADR in 72 h were compared among 4 groups after inoculation;venous blood of chil-dren was collected before vaccination and 5 months after vaccination,and the antibody positive test was carried out by micro coagu-lation inhibition (HI) test;HI antibody titer was recorded after immunization,and positive rate and genometric meantiter(GMT) were calculated. RESULTS:The incidence of ADR in 4 groups were 9.33%,8.00%,8.00% and 10.67%,respectively. No local ADR was found in 4 groups;among systemic ADR,the incidence of fever was higher than that of other clinical manifestations,be-ing 4.00%,4.00%,4.00% and 5.33%;there was no statistical significance in the incidence of ADR among 4 groups(P>0.05). Measles,mumps and rubella antibody positive rates of MMR group were 100%,92.00% and 100%,respectively;antibody posi-tive rates of measles vaccine group,mumps vaccine group and rubella vaccine group were 100%,85.33% and 100%,respective-ly;there was no statistical significance in same antibody positive rate among 4 groups(P>0.05). GMT of measles in MMR group and measles vaccine group were 1∶41 and 1∶27,that of mumps in MMR group and mumps vaccine group were 1∶6.3 and 1∶6.2, there was no statistical significance (P>0.05);GMT of rubella in MMR group and rubella vaccine group were 1∶320 and 1∶849, with statistical significance (P<0.05). CONCLUSIONS:Compared to traditional single vaccine,MMR dose not increase the inci-dence of ADR and not influence positive rate,but GMT of rubella increases significantly,to which should be paid attention.

During the past few years,mounting evidence has proved that the protection induced by vaccines or the prognosis of diseases is correlated with the quantity and quality of T cell responses in both mouse models and human infectious diseases.Examination of one or a few effector functions might not fully reflect the antigen-specific immunity.Recent studies demonstrated that after antigen stimulation,T cells could be induced to produce two or more effector functions simultaneously at single cell level,including cytokines,chemokines production and cytotoxic related molecules,which are named as polyfunctional T cells.Further studies indicated that these polyfunctional T cells are linked to protection from infectious diseases.Therefore,measuring multiple functions of T cells simultaneously is essential for the proper evaluation of specific immunity and may have substantial implications for the design of vaccines.

The causes of Cd-induced anemia and the preventive effect of Fe, Zn or Cu was studied. Day-old male chicken were randomly divided on body weight to 6 groups:A, Basal diet; B, Basal diet + Cd; C, Basal diet + Cd + Fe; D, Basal diet + Cd + Zn, E, Basal diet + Cd + Cu, F, Basal Diet + Cd + Fe + Zn + Cu. After 3 weeks feeding, the parameters to evaluate anemia of the chicken were observed.The results indicated: 1. Dietary Cd produced normocytic hypochro-mic anemia in chicken. Its causes were related to the abnormal size and decreased resistance of RBC and the deficiency of Fe and Cu in the chicken. 2. Dietary supplement of Fe and Cu was able to prevent Cd-induced anemia, but Zn had no effect. 3. Dietary Cd produced growth retardation in chicken and it could be prevented by supplementing Fe, Zn or Cu.

The blood levels of hemoglobin(Hb), cerrulo-plasmin (Cp), superoxide dismutase (SOD), glu-tathion peroxidase (GSH-Px), malonaldehyde(MAD) and the concentrition of Cd, Fe, Cu andSe in liver were measured 4 weeks after weanedSprague-Dawley rats fed on Cd-supplemented diet(Cd 100mg/kg). The results indicated that. theconcentrition of Fe, Cu and Se in liver and theblood levels of Hb, SOD and GSH-Px activity weredecreased, but the blood level of MAD was in-creased by dietary Cd. The abnormal changes andanemia induced by Cd were able to be partially al-leviated by oral supplementing Fe, Cu and Se. Itsuggested. that Cd was able to produce peroxidationof erythrocyte membrane lipids. It is a possiblemechanism of the decreased survival of erythrocyteand hemolysis by Cd.

Objective To increase the appreciation of manifestations in autoimmune pancreatitis (AIP) and to decrease misdiagnosis rate by investigating the clinical characteristics of AIP. Methods Clinical data were collected and laboratory ,imaging and histopathology were analyzed from the Chinese PLA General Hospital from 1995 to 2009. Patients with AIP were included in the study. Results Eight patients (male 6, female 2) aged 35-69 (52.4 ±9.4) years were diagnosed as AIP from 2006 to 2009. The main clinical manifestations include intermittent jaundice in 6 cases (6/8), abdominal pain in 5 cases (5/8),weight loss in 4 cases (4/8), and accompanied with other diseases of immune system in 4 cases (4/8).The imaging showed head of pancreas enlargement in 3 cases (3/8) and whole pancreas enlargement in 5 cases (5/8). There is a "banana-peel like" ring around the pancreas and irregular stenosis of pancreatic duct. Massive lymphocytes and plasma cells infiltration and parenchymal fibrosis were shown in pancreatic tissues, bile duct,salivary gland and liver in pathology. Clinical manifestations, laboratory examinations and images in 7 cases (7/8) were improved after treatment with prednisone. Twenty-two of them were misdiagnosed as pancreatic or biliary carcinoma and 21 were performed laparotomy in the period of 1995-2005 ( 23 cases). After 2006, however, the misdiagnosis rate significantly decreased from 95.7% ( 22/23 ) to 0. Conclusions AIP seems to be a systemic autoimmune disease rather than an isolated disorder,markedly overlapping with other autoimmune diseases. Definitive diagnosis can be improved by the detection of immune parameters and pathological examination.

Through PubMed, CNKI and other databases to retrieve original documents about the maintenance treatment of advanced colorectal cancer, collect and analyzed research theory and progress in the maintenance treatment of advanced colorectal cancer. The clinical efficacy of maintenance therapy are evaluated and the patient's disease-free survival and quality of life are observed. The Result shows that the maintenance treatment can improve disease-free survival of advanced colorectal cancer patients, and the toxicity of maintenance treatment can still be tolerated. So the maintenance treatment of advanced colorectal cancer has better clinical efficacy, it is worth to study in the future.

ObjectiveTo investigate the correlation of TIMI risk score and plasma brain natriuretic peptide (BNP) in patients with Acute myocardial infarction. MethodsTIMI risk score were calculated in 63 patients with acute myocardial infarction;At the same time plasma brain natriuretic peptide were measured by enzyme-linked immune absorption assay(ELISA). Analyze the correlation of TIMI risk score and plasma brain natriuretic peptide. ResultsNine patients of 63 occurred cardiavascular events during hospitalization or follow-up( 14.3％ ). The incidence of MACE increased significantly with the rise of TIMI risk score and BNP level. The TIMI risk score was positively correlated with the plasma BNP level( r =0.73 ,P ＜ 0. 05 ). ConclusionThe higher TIMI risk score and BNP level, the worse the prognosis, it could be use to predict the prognosis.

Objective To study the influence of aging and chemotherapeutic drugs of breast cancer patients on chemotherapy-induced amenorrhea (CIA) and to guide the further selection of drugs for chemotherapy.Methods We prospectively studied the CIA of the premenopausal breast cancer patients and compared the difference in CIA between the patients who were at different aging periods or received different chemotherapeutics. Results 137 patients were followed up.The occurrence rate of CIA and long-term chemotherapy-induced amenorrhea(LCIA)were 73.72%(101/137)and 43.80%(60/137)respectively.The rate of CIA and LCIA was significandy higher in older women(＞40 years)than young women(≤40 years,X2=25.32,18.42,P＜0.05).The recovery rate of CIA in young women was 61.90%(13/21)which was significantly higher than the older women[35.00%(28/80);X2=4.99,P=0.025].The difference in the rate of LCIA was statistically significant between the older women (＞40 years)who received different anthracyclinebased chemotherapy(X2=6.92,P=0.031).Conclusion Age is an important factor for the CIA.Chemotherapy has less influence on the menstruation of younger women(≤40) and most amenorrhea is reversible.For older women,the rate of LCIA is different in patients who receive imported epirubicin,domestic epirubicin or pirarubicin.