Objective To investigate the infectious state and drug resistance of Haemophilus par lainfluenzae in respiratory tract infection, so as to instruct the rational use of antibiotics. Methods Haemophilus par lainfluenzae were isolated from the pa-tients with respiratory tract infection and performed by K-B methods. Nitrocefin slip test was applied into detecting β-lactamase. Re-sults A total of 45 Hacmophilus par lainfluenzae were isolated from 760 specimen, with the isolation rate being 5.92%. Amoxicil-lin/claulanic acid,Azithromycin, Imipenem,Ceftazidime and Cefuroxime had the better antibiotic function and the average drug re-sistance was lower than 5 % ,The β-lactamase positive rate was 37.78 %. Conclusion The high drug resistance and β-lactamase pos-itive rate of Haemophilus par lainfluenzae isolated from respiratory tract characterized by multi-resistance must be taken seriousely.

This article analyses the chemical test results of several common repair mesh, and concludes the currently several common repair mesh are safe and reliable.
Prostheses and Implants ; Surgical Mesh ; standards

The cognitive conflict between doctors and patients mainly manifests in the different cognition of diseases,the expectation of medical skill and the understanding of medical science.The way to dissolve the cognitive conflict between doctors and patients lies in strengthening exchange and communication between them.

Animals ; Cell Culture Techniques ; Cell Differentiation ; Cell Proliferation ; Embryonic Stem Cells ; cytology ; metabolism ; Germ Cells ; cytology ; metabolism ; Germ Layers ; cytology ; metabolism ; Humans ; Induced Pluripotent Stem Cells ; cytology ; metabolism ; Mice ; Pluripotent Stem Cells ; cytology ; metabolism ; Reproductive Techniques, Assisted

Objective To investigate the differences in expression of hypoxia-inducible factor-1? (HIF-1?) in placental trophoblasts between normal pregnancy and pregnancy-induced hypertension (PIH) and the relationship of HIF-1? expression with magnesium sulfate treatment of PIH. Methods Immunohistochemical streptavidin biotin-peroxidase complex technique (SABC) was used to examine the expression of HIF-1? in 20 cases of PIH and 20 cases of normal placenta. Three groups of PIH were divided according to the degrees of severity. The results were analyzed by computer-assisted imaging and compared by t-test. Results There was significant difference in the expression of HIF-1? between the PIH group and the control group (P

Herbal drug-induced liver injury is a hot issue in the fi eld of TCM researches.Rapid screening and evaluating of herbal drug-induced liver injury have become one of the key techniques of herbal research.This text carried on the discussion on the research status of it from active,toxic ingredient,toxic mechanism and decreasing toxicity of Chinese herbal drugs.At the same time,the text viewed the application foreground of gene chip technique in the study of it.The current status and methods of it was discussed.In addition,most of the reports adopted in this paper were about studies on crude drugs,and discussion on how to correctly treat herbal drug-induced liver injury.

Acupuncture Points ; Acupuncture Therapy ; methods ; Terminology as Topic ; Translating

Objective:To investigate the acid resistance of tooth hard tissues irradiated by different power outputs of Er,Cr∶YSGG laser.Methods:Samples in laser groups were irradiated using Er,Cr∶ YSGG laser with the power outputs of 2.5,3.5 and 5 W for enamel and 2 W,3 W and 4 W for dentin,respectively.The calcium and phosphate ion dissolved was measured after decalcification in lactate buffer solution for 24 h.The atomic percentage of calcium and phosphate on the surface of samples was examined by X-ray energy dispersive spectroscopy and the morphological changes were investigated by SEM.Results:In all laser groups of enamel samples except 2.5 W group calcium and phosphate ion dissolved less than in control group and block group (P＜0.05).There's no statistic difference between different power groups.Compared with control group and block group,Ca/P ratio increased(P＜0.05).There's no statistic difference of the atomic percentage of Ca and P on the surface of dentin samples between each 2 groups.SEM observation showed that the surface of the laser irradiated samples was rouph,the space among enamel fibers was increased,the dentin around dentinal tuble orifice was protruded and looked like collar flange.Conclusion:Er,Cr∶YSGG laser irradiation with a range of power is effective in increasing acquired acid resistance of dental hard tissue.There was no relationship between laser power outputs and acid resistance.

Objective To investigate different scoring systems in predicting the severity of acute pancreatitis(AP).Methods The clinical data of 1 56 patients with AP were retrospectively reviewed.Ser-um c-reactive protein(CRP)levels were measuredat admission.According to the Chinese guidelines for the management of acute pancreatitis(2007),all the patients were categorizedas either mild acute pancre-atitis(MAP)or severe acute pancreatitis(SAP).Ranson,acute physiology and chronic health evaluation (APACHE)-Ⅱ,bedside index for severity in acute pancreatitis(BISAP),and computed tomography se-verity index(CTSI)scoring systemswere calculated according to the corresponding grading standardsin all patients.Patients were divided into MAP group(APACHEⅡ <8,Ranson <3,BISAP <2,CTSI <3 and CRP <21 .4)and SAP group (APACHEⅡ≥8,Ranson≥3,BISAP≥2,CTSI≥3 and CRP≥21 .4)ac-cording to the scoring results.ROC curve was used to compare the difference among the systems.Results Among the 1 56 patients,21 (1 3.5%)were classified as SAP and 1 35 as (86.5%)MAP.AUCs for Ranson,BISAP,APACHEⅡ,CTSI,and CRP in predicting SAP were 0.69 (95%CI:0.62-0.76),0.74 (95%CI:0.66-0.80),0.78 (95%CI:0.70-0.84),0.69 (95%CI:0.61 -0.76),and 0.68 (95%CI:0.57-0.78),respectively.There were no significant differences among these scoring systems.Conclusion There were no significant differencesin predicting the severity of AP among these scoring systems. Therefore,the early prediction of SAP should consider multiple scoring systems,and the referential signifi-cance of accessing and applying a simpler laboratory indicator deserves further studies.

Objective To investigate the impact of Ruai-Kangfu decoction on the quality of life of the patients with breast cancer after operation.Methods A total of 107 patients with breast cancer who met the inclusion criteria were randomly divided into the control group (54 patients) and observation group (53 patients). The control group was treated with the radiotherapy and chemotherapy and symptomatic support treatment. The observation group was treated with the Ruai-Kangfu decoction on the basis of the control group. The 2 groups were treated for 12 months, followed up for 6 months. The European Organization for research and treatment of cancer life quality scale (quality of life questionnaire-core 30, QLQ-C30) and the living quality of breast cancer (quality of life questionnaire-breast table cancer module 23, QLQ-BR23) were used for assessing the quality oflife of the patients.Results After treatment, the scores of QLQ-C30 scale the body function (76.4 ± 16.6vs. 70.4 ± 16.4,t=-2.087), role functions (59.2 ± 12.8vs. 54.6 ± 12.8,t=-2.085), emotional function(77.2 ± 17.0 vs. 54.7 ± 12.8,t=-2.075), cognitive function (38.5 ± 14.7 vs. 34.3 ± 11.6t=2.104), social function(59.3 ± 12.4 vs. 54.6 ± 12.8,t=-2.105) and the overall health status (76.7 ± 19.7 vs. 65.5 ± 18.6,t=-2.152) in the observation group were significantly higher than those in the control group (P<0.05). The weary (40.9 ± 8.4 vs. 45.3 ± 9.6, t=2.149), nausea and vomiting (42.9 ± 8.8 vs. 44.9 ± 10.1,t=2.145), pain (36.9 ± 7.6 vs. 40.9 ± 8.7,t=2.143), dyspnea (44.8 ± 9.2 vs. 49.6 ± 10.6,t=2.148), insomnia (41.8 ± 8.6 vs. 46.3 ± 9.9,t=2.152), loss of appetite (35.0 ± 7.2 vs. 38.7 ± 8.2,t=2.113), constipation (15.9 ± 3.3 vs. 17.6 ± 3.8,t=2.057), diarrhea (15.2 ± 3.1 vs. 16.9 ± 3.6, t=2.092) scores in the observation group were significantly lower than those in the control group (P<0.05). The scores of QLQ-BR23 scale body type (74.7 ± 18.5 vs. 64.8 ± 15.4,t=-2.153), the function of sex (36.3 ± 9.0 vs. 42.8 ± 10.2,t=2.045), sexual fun (41.4 ± 10.2 vs. 48.9 ± 11.6,t=2.046), view of the future (67.6 ± 16.7 vs. 58.6 ± 14.0,t=-2.132), side effects (48.2 ± 10.4 vs. 55.2 ± 12.2,t=2.149), breast symptoms (39.6 ± 8.6 vs. 45.5 ± 10.2, t=2.127), arm symptoms (30.3 ± 6.6 vs. 34.8 ± 7.7,t=2.129), trouble from alopecia (36.1 ± 7.8 vs. 41.4 ± 9.1, t=2.113) in the observation group were significantly higher than the control group (P<0.05).Conclusions The Ruai-Kangfu decoction can adjust the mental status and improve the quality of life of patients with breast cancer after operation.