OBJECTIVE:To determine the serum concentration of Deoxyschisandrin by RP-HPLC.METHODS:The chr-omatographic separation was performed on Phenomenex C18(250mm?4.6mm,5?m) column with column temperature at 30℃.The mobile phase consisted of methanol - water (80∶20) at a flow rate of 1mL?min-1. The detection wavelength was set at 252nm. RESULTS:The calibration curve of Deoxyschisandrin was linear in the concentration range from 0.52 to 33.28?g?mL-1 (r=0.999 6). The average recovery of deoxyschisandrin was (97.27?4.11)%(RSD=4.23%,n=9).CONCLUSION:The method is simple, accurate and sensitive, and it can be used for the determination of serum concentration of Deoxyschisandrin.

An embryo of 4.3 mm is described. It comes to the laboratory in 10 per cent for-malin as an intact chorion measuring 22 by 16 mm. The embryo is in the shape of theletter C, and its tail is curved. The limb buds are round in shape. There are 4pharyngeal pouches, the 4th being very small. Between the bases of the 1st and 2ndbranchial arches, in the floor of the pharynx, the tuberculum impar has developed. Theotic vesicles are slightly elongated sacs and no longer connected with the covering ecto-derm. The eyes are represented by the optic vesicles and the thickened ectodermal an-lage of the lens. Trachea is still largely a groove in the ventral wall of the pharynx andesophagus. The heart is still in the so-called S shaped stage; the atrium lies on the leftside of the bulb, and has not yet doubled; the sinus venosus is not completely out ofthe septum transversum; in the atrium, near the sinus venosus, the endothelial tube isclosely attached to the wall. There are 4 pairs of aortic arches: the lst pair breaks intocapillaries imbedded in the mandible. The other three join the dorsal aortae which runcaudal and finally unite to form one median dorsal aorta. The anterior cardinal, pos-terior cardinal, common cardinal and vitelline veins, etc. are all paired and symetricallyarranged. The embryo herein described falls in group Ⅷ of Streeter.

ObjectiveTo observe the effect of two kinds of combination of acupuncture points on postoperative enteroparalysis.Methods96 patients with postoperative enteroparalysis were divided into the group A (n=44) and group B (n=52). The patients of the group A were treated with acupuncture on Mu points and lower-he point, while those of the group B on Back-shu points and distant lower-he point. The therapeutic effect of two groups was compared.ResultsThe two therapeutic methods had good effect, but the Back-shu points and distant lower-he points was superior to the Mu points and distant lower-he points ( P<0.05).ConclusionAcupuncture with combination of special points has better effect on postoperative enteroparalysis.

Objective To evaluate the value of plasma soluble urokinase?type plasminogen activator receptor (suPAR)and D?dimer for assessing disease severity and prognosis in patients with traumatic brain injury(TBI). Methods A serial of 112 patients with traumatic brain injury admired to our hospital were divided into mild TBI (GCS score 13~15),moderate TBI(GCS score 9~12)and severe TBI(GCS score 3~8). The levels of plasma suPAR and D?dimer were monitored within 24 h after patient admission. Results The levels of suPAR and D?di?mer in TBI group were obviously higher than those in the control group(15.86±7.33 vs 2.79 ± 0.69,P<0.01;3.50 ± 2.78 vs 24 ± 0.15,P<0.01). The levels of suPAR and D?dimer in the severe TBI group were also obviously high?er than those in the moderate TBI group(P<0.05). Plasma suPAR levels and GCS score had significant negative correlation(r =- 0.854,P < 0.01). D?dimer levels and GCS score showed a negative correlation(r =- 0.738, P < 0.01). Plasma suPAR and D ? dimer was positively correlated(r = 0.753,P < 0.01). The area under the curve of D?dimer(AUC)was larger,0.854,95%CI 0.763~0.945;the AUC of suPAR was 0.801,95%CI 0.698~0.903. Conclusion Higher levels of suPAR and D?dimer in TBI patients is not only relevant to the injury sever?ity ,but also closely to the prognosis.

Objective:To assess the compliance and efficacy of mirtazapine or armitriptyline in the maintance treatment of depression. Methods:60 depressive outpatients were randomly allocated to two groups treated with mirtazapine or armitriptyline for 52 weeks. Before treatment and at the ends of 12 weeks and 52 weeks of treatment, to assess the efficacy using the Hamiltion Depression Scale (HAMD)and Clinical Global Impression(CGI),to assess the compliance and the adverse-effects using the Hamilton Depression Scale (HAMD)and Clinical Global Impression(CGI),to assess the compliance and the adverse-effects using Treatment Emergent Symptom Scale (TESS).Results:After 12 weeks, significant improvement was observed respectively in the two groups(P

Objective To study into the influence of Ze Xie and Dan Shan root on the immunity regulation and the expression of radical in mice with chronic pelvic infection. Methods The model of mice with chronic pelvic infection was established by putting phenol into the endometrium of the mice. The density change of serum IL -2, TNF, SOD and MDA of the mice was measure by the method of radiation immunity and the method of biochemistry. Results The expression of serum IL - 2 and SOD in Ze Xie and Dan Shan root group was obviously higher than that in the model group( P

Objective:To establish a fluid dynamics model of upper airway before and after surgery and explore the changes of three-dimensional fluid dynamics in patients with micrognathia.Methods:A patient with micrognathia and severe obstructive sleep apnea-hypopnea syndrome(OSAHS)accepted CT scan before and six months after mandibular advancement operation.Computation-al fluid dynamics model was built on the base of CT scan by Mimics 1 0.01 and ANSYS ICEMCFD1 4.0.The internal flow of upper respiratory tract was simulated by ANSYS-FLUENT 1 4.0 and the results were analyzed by Tecplot.Results:Fluid dynamics model of upper airway was constructed before and after the surgery respectively.The volume of the upper airway of the patient increased from 37.284 cm3 to 44.498 cm3;the most narrow area of upper airway was located in the lower bound of pharyngopalatiae,and it was augmented from 1 .1 35 cm2 to 2.297 cm2;the minimum pressure was decreased from1 01 308 Pa to 1 01 272 Pa;the maximum air velocity increased from 3.476 m/s to 4.978 m/s.Conclusion:Mandibular advancement may correct the occlusal deformity,ex-panse the upper respiratory tract,decrease the negative pressure and maintain the patency of the airflow in the treatment of patients with micrognathia and OSAHS.

Objective To investigate the mechanism of a dipeptidyl-peptidase-4 (DPP-4) inhibitor, saxagliptin, pro?moting the regeneration of islet beta cells in diabetic rats. Methods The male SD rats were randomly divided into three groups including control group (NC, n=10), diabetes group (DM, n=10) and diabetes treated with saxagliptin group (DM-S, n=10). DM-S group was treated with saxagliptin 1 mg/(kg·d) for twelve weeks. The pancreaticβcell function was analysed by hyperglycemic clamps. Immunohistochemistry with anti-PCNA was performed to observe the proliferation rate of pancreaticβcells. Immunofluorescence double staining with anti-insulin, anti-glucagon, anti-DPP-4 and anti-SDF-1 were performed to observe the expression of insulin, glucagon, DPP-4 and SDF-1 in pancreatic tissue. Western blot assay was performed to test the expression of Akt, p-Akt,β-catenin and free-β-catenin protein, and RT-PCR was performed to test the expressionlevels of c-myc and cyclinD1 mRNA in pancreatic tissue. Results Compared with NC group, there were significantly in?creased blood glucose, decreased islet function andβcell mass in DM group. Compared with DM rats, saxagliptin treatment significantly inhibited the expression of DPP-4, decreased the degradation of SDF-1, stimulated the proliferation ofβcells, and ultimately improved the islet function and histopathological changes of pancreas. Conclusion DPP-4 inhibitor saxa?gliptin can significantly improve islet function, which involved in the inhibition of the expression of DPP-4, the decreased degradation of SDF-1 and the stimulation of the proliferation ofβcells.

Objective To investigate the possible mechanisms of glucagon-like peptide 1 receptor agonists (GLP-1Ra) induced weight loss. Methods High fat diet induced obese c57BL/6 mice were divided into normal control group (N, n=8), high fat feeding group (HF, n=32) and GLP-1Ra group treated with GLP-1Ra (liraglutide 200μg/(kg·d) or 400μg/(kg·d) for 8 weeks). Changes of body weight, blood glucose and three acyl glycosides (TG) levels were observed in three groups. HE staining was used to observe the morphological changes. Immunofluorescence staining and real-time PCR were used to mea?sure the expression of UCP-1. Furthermore, the expression of PGC-1αin protein level was observed to explore the possible mechanism of GLP-1Ra induced browning in white fat (WAT). Results After 8-week liraglutide (Lira) administration, the body weights were significantly reduced in obese mice (P<0.05). The levels of blood glucose and TG were significantly high?er in HF group than those in N group, which reduced significantly in Lira (200μg·kg-1) and Lira (400μg·kg-1) administra?tion groups (P<0.05). HE staining showed adipocytes in perirenal and inguinal subcutaneous adipose tissue partly acquired brown-like morphological characteristics. The expression levels of UCP-1 protein and mRNA and PGC-1αprotein were ele?vated in adipse tissues, which increased more in Lira (400) than those in Lira (200, P<0.05). Conclusion GLP-1Ra can induce weight loss through white fat browning by activation of UCP-1.

ObjectiveTo investigate the relationship between the level of plasma D-dimer(D-D) and hepatic functional reserve status in patients with hepatitis B (HB).Methods77 cases with HB were divided into two groups, hepatic function compensated group and hepatic function decompensated group.Plasma D-D, hepatic functional indexes and PT of all patients were detected.ResultsThe level of D-D in the hepatic function decompensated group was higher than that in the hepatic function compensated group(299.0 ± 498.5) μg/L vs (27.9 ± 34.0) μg/L(t =3.1, P ＜ 0.01).D-D negatively correlated with ALB and PA(r =-0.6,-0.6, all P ＜ 0.01), and positively correlated with TBIL and PT(r = 0.4,0.6, all P ＜ 0.01).ConclusionThe level of plasma D-D related with hepatic functional reserve status,and detecting the level of D-D could be helpful to estimate hepatic functional reserve and prognosis of patients with HB.