The characteristics of knowledge exchange in medical field of China were analyzed by social network analysis with the medical data covered in CNKI Citation Database as its study object, which showed that both the inflow and outflow volumes of clinical medicine are the largest and form the core in knowledge exchange network, preclinical medicine is the theoretical base of other medical subjects and the important knowledge source in medical networks, the interchange is close between traditional Chinese medicine, surgery and other clinical subjects, oncology is rather active in interdisciplinary studies, the interchange between stomatology and psychology is rather rare with other clinical subjects in knowledge networks and stomatology and psychology are two independent subjects.Carrying out information push service for these subjects by making use of their characteristics can improve the targeted information service, step up the knowledge exchange between different medical subjects, and promote the cooperative interdis-ciplinary studies.

Objective To discuss the ultrasonographic features of immunosuppressants induced hepatotoxicosis after renal allograft. Methods The pre operative and postoperative ultrasonographic features of liver and gallbladder (GB) of 29 renal transplantation patients with clinical hepatotoxicity, after the use of CsA as the main immunosuppressive agent, were evaluated. Results Out of the 29 patients, 23 exhibited enhanced liver echo and 6 patients had features of fatty liver. The thickness of gallbladder wall was around 0.3cm in 21 patients. The morbidity rate of hepatotoxicity was 69% (20/29) in three months. Conclusion The main ultrasonographic features of immunosuppresive agent induced hepatotoxicity were enhanced liver echo, thickened gallbladder wall and unclear echo from GB. These findings provided important subsidiary diagnostic signs for hepatotoxicosis, especially three months after renal allograft. At this time, not only the allograft renal should be examined, but also the liver and gallbladder should be scrutinized with ultrasound. The ultrasonographic changes together with the clinical laboratory data could serve as a guidance for the administration of immunosuppressive drugs.

Objective To explore the risk factors of death in infant with muggy syndrome(IMS).Methods Sixty-three children with IMS were analyzed retrospectively, who admitted from Jan. 2002 to Feb. 2007,and these children were divided into groups according to the presence or absence of combined conditions including metabolic acidosis, hyperglycemia, hypocalcemia, ultrahyperpyrexia, pediatric critical illness score(PCIS) or number of the multiple organs failure. The ?2 test was used to examine the mortality between those groups.Results The total mortality of these children with IMS in-hospital was 22.1%. There were significant differences in mortality between combining metabolic acidosis and non-combining ones (RR=3.20,95%CI=1.0-10.24 ?2=4.76 P80 score (RR=12.73,95%CI=1.78-91.04,?2=13.24 P12 mmol/L and those

OBJECTIVE: To review the pathological and clinical features and treatment of sinonasal malignant melanoma. METHOD: A retrospective analysis of 23 cases of sinonasal malignant melanoma. All the patients were conformed by histopathology, the most common symptoms were nasal obstruction and epistexis. Eight cases were treated with surgery, 8 with surgery and radiotherapy, 5 with surgery and chemotherapy, 2 with surgery and ra- diotherapy plus chemotherapy. RESULT: Twenty patients were followed up, the survival rates of 3 and 5 years were 50% (10/20) and 35% (7/20), respectively. CONCLUSION Sinonasal malignant melanoma has an aggressive behavior and easy recurrence and poor prognosis. Early diagnosis and reasonable treatment can increase the survival rate of the disease.
Humans ; Melanoma ; mortality ; pathology ; therapy ; Nasal Obstruction ; Neoplasm Recurrence, Local ; Nose Neoplasms ; mortality ; pathology ; therapy ; Paraganglioma ; Paranasal Sinus Neoplasms ; mortality ; pathology ; therapy ; Retrospective Studies ; Survival Rate

OBJECTIVE To study the proteome of inferior colliculus and determinate the region-typical proteins which may be the candidate cause of the Central Auditory Processing Disorders. METHODS The telencephalon was taken as reference, and then identified and quantified the proteome of IC of adult rats with iTRAQ. Those with higher abundance in inferior colliculus than the other three regions were considered as IC-Region typical proteins,which may lead to functional specializations. RESULTS We identified 1937 cytomembrane proteins in total, among which there are 53 IC-Region typical proteins, which may lead to functional specializations of inferior colliculus.We used GO and KEGG pathway to analyze these proteins and then found that these proteins mainly take part in the regulation of neurons development and information integrations. CONCLUSION Our quantitative comparison of inferior colliculus has revealed two candidate proteins, including CaMKII and SV2A, which may play important roles in maintaining the balance of excitatory and inhibitory transmitters release. These proteins may be the candidate proteins for Central Auditory Processing Disorders.

Objective To investigate whether the splenic metastasis of B16 melanoma cells in C57BL/6 mice occur through the blood.Methods Cultured B16 melanoma cells were collected,adjusted into 1?10~7/ml and inoculated into the left armpit of C57BL/6 mice,200 ?l each.After 15 days,the mice were sac- rificed and metastasis was examined in various tissues and organs by HE stain.1.5 % Evan Blue(EB),2 ml/kg, was administered to normal C57BL/6 mice through the tail vein,and the region stained blue was compared with that of metastasis.Results All of 12 mice inoculated were burdened with melanoma.Splenic metastasis occurred in 9 of them,among which 3 were accompanied by lung metastasis,and all of 9 mice showed differ- ent degree of local lymph node metastasis.The dorsal 1/4 part of the positive spleen was stained black by the metastatic tumor cells.Under the microscope,distribution of the metastatic tumor cells in the spleen was scat- tered,their growth inhibited,and maturation accelerated.The region stained blue by EB also located at the dorsal 1/4 part of the spleen,which was almost the same as the positive part of metastasis.Conclusions Metastasis of B16 melanoma cells in C57BL/6 miee occurs earlier in the spleen than in other organs,and this metastasis is achieved most possibly through the blood.

Adolescent ; Adult ; Aged ; Aged, 80 and over ; Child ; Dermatomyositis ; diagnosis ; Female ; Humans ; Male ; Middle Aged ; Otolaryngology ; Young Adult

Objectives To evaluate the diagnostic value of real-time quantitative fluorescence polymerase chain reaction( Q-RT-PCR ) assay and immunofluorescence assay for diagnosis of hMPV. Methods Totally 1 283 children with acute respiratory infection admitted in Jiaxing Maternity and Child Health Care Hospital for treatment from November 2008 to May 2009 were recruited in this study. The hMPV positive stains were separated and sequenced in this area. The sequences between the local hMPV stains and Holland stains NLD00-1 were compared. The specific primers and fluorescent probe were designed according to the sequence of epidemic hMPV strain. The Taqman methodology was applied in Q-RT-PCR. Negative pressure suction was used to acquire nasopharyngeal secretions specimens. Both Q-RT-PCR and immunofluorescence with FITC labeled monoclonal antibody were used to analyze them, respectively. The McNemar, test was applied to analyze the correlation between the two methods. Results Totally 1 283 specimens were analyzed with Q-RT-PCR and immunofiuorescence simultaneously. Q-RT-PCR analysis showed there were 59 cases positive. Immunofluorescence analysis showed there were 55 cases positive. Fifty-two cases were positive in both assays. There were 7 cases positive in Q-RT-PCR assay but negative in immunofluorescence assay and 3 cases negative in Q-RT-PCR assay but positive in immunofluorescence assay. If Q-RT-PCR method was set as the golden standard, the sensitivity and specificity for immunofluorescence detection method were 88. 1%and 99. 8%, respectively. Positive predictive value and negative predictive value were 94. 5% and 99. 4%,respectively. There was no significant difference ( χ2= 0. 9, P ＞ 0. 05 ) by McNemar' test between the two methods. Conclusion The diagnostic value of immunofluorescence assay is close to Q-RT-PCR assay.

Objective To investigate the clinical efficacy of acupuncture plus ashi point pricking bloodletting in treating acute gouty arthritis.Methods Seventy patients with acute gouty arthritis were randomly allocated to a treatment group of 38 cases and a control group of 32 cases. The treatment group received acupuncture plus ashi point pricking bloodletting and the control group, routine medication. Blood uric acid and C reaction protein contents were measured in the two groups before and after treatment. The clinical therapeutic effects were compared between the two groups.Results There were statistically significant differences in blood uric acid and C reaction protein contents after one and two courses of treatment compared with before treatment in the two groups (P<0.01). After two courses of treatment, there was a statistically significant difference in comparing C reaction protein contents between the two groups (P<0.05). The total efficacy rate was 84.2% in the treatment group and 81.3% in the control group; there was a statistically significant difference between the two groups (P<0.05).Conclusion Acupuncture plus ashi point pricking bloodletting is an effective way to treat acute gouty arthritis.

Objective To investigate clinical value of procalcitonin (PCT ) in guiding treatment of infection in elderly patients with heart failure .Methods A total of 120 cases of elderly patients with heart failure and infection were divided into control group (50 cases) ,receiving conventional anti-infection treatment ,and observation group(70 cases) ,receiving adjusted anti-infection treat-ment based on serum PCT levels .Treatment efficiency ,cost of treatment ,hospitalization duration and other indicators were com -pared between the two groups .Results Effective rate of observation group was 78 .57% ,which was not significantly different with the 78 .00% of control group ( P > 0 .05) .Cost of treatment ,hospitalization duration ,application time of antibiotics ,antibacterial drug costs ,superinfection and mortality were significantly different between the two groups (P< 0 .05) .After treatment ,levels of e-rythrocyte sedimentation rate ,C-reaction protein ,white blood cells and neutrophil in the two groups were significantly improved (P< 0 .05) .After treatment ,C-reaction protein level of observation group was lower than control group (P < 0 .05) .Conclusion PCT based anti-infection treatment could help shorten the treatment time ,reducing treatment costs and improving outcomes ,and might be worthy of clinical application .