Vaccination in children suffering neurological diseases is a troublesome issue that people es-pecially pediatricians are reluctant to mention. Due to worrying about the emergence of adverse reactions and concerning on the heat induced seizure,doctors and parents tend to cancel or do not recommend these children to be vaccinated. Additionally,the heat sensitive seizures,such as febrile seizure( FS) ,generalized epilepsy with fe-brile seizure plus(GEFS+),Dravet syndrome account for the largest proportion of the seizure related disease and epilepsy syndrome. Their pathogenesis has been proved relating to the mutation of SCN gene of the sodium channel. For children with FS,GEFS+,and Dravet syndrome,vaccination may lead to fever,which even may bring about convulsions,but it will not result in worse prognosis.

OBJECTIVE:To classify the herbs for promoting blood circulation to remove blood stasis.METHODS:Database of 33 commonly used herbs for promoting blood circulation to remove blood stasis was established by means of SPSS10.0 software package;and the nature,tastes,meridian attributions and actions of the herbs were collected as variables showing the characteristics of classification,and the names of the herbs served as variables for measurement to proceed Q Cluster in Hierarchical Cluster.RESULTS:When the herbs were classified into 4 groups,the first group included the herbs for promoting blood circulation to restore menstrual flow,the second the herbs for promoting blood circulation to arrest pain and dissipate tumor,the third the herbs of herbs for activating blood circulation to dissipate blood stasis and kill pain,the fourth the herbs for severely activating blood circulation to dissipate blood stasis.When the herbs were classified into 5 groups,the fifth group included herbs for activating blood circulation to cure wound.When the herbs were classified into 6 groups,the third one included the herbs for activating blood circulation to exclude wind and the sixth the herbs for activating blood circulation to stanch blood.CONCLUSION:Fuzzy grouping analysis can be applied to classify herbs of different kinds yet with the same efficacy and to classify the same group of herbs according to their drug actions.

Objective To explore the effects of neuronal apoptosis in the hippocampal CA 1 region by the selective nitric oxide synthase(NOS) inhibitor, 7 nitroindazole (7 NI).Methods 72 postnatal 7 day old Wistar rats were randomly divided into 3 groups.(1)Sham operation group:12 rats were cut in their neck centre without ligature in common carotid artery;(2)Obersevertion group(7 NI group):the abdominal cavity of 30 rats were injected intraperitoneally into 7 NI (25 mg/kg) after anoxic ischemic damaged models were made;(3)The control group(the normal saline):30 rats were injected intraperitoneally into the normal saline the same volume after HIBD.The sham operative group was later than the operative one.The rats in the observation group and the control one by killed later,they were cut heads and brains in 48h.and were detected by HE and TUNEL stainning,the numbers of cerebral apoptosis were detected under the lightmicroscope.Results Typal apoptotic cells in the hippocampal CA 1 region in the control group were detected by HE stainning. They showed cell pyknosis,cytoplasm deep stain,nucleus concentration,fragment,karyoplasm unclear,apoptosis corpuscle was formed;in 7 NI group the above apoptosis decresed significantly;There was significant difference between Group 2 and Group 3(TUNEL positive apoptotic cells in Group 2)( P

Objective To understand brucellosis infective status of Shapotou District, Zhongwei City of Ningxia. Methods The methods of stratified random sampling of epidemiological survey were used among high risk groups in 2008, who underwent erythromycin red plate cohesion test(RBDT) and test-tube agglutination test (SAT), in accordance with the diagnostic criteria for confirming cases of brucellosis(GB 15988-1995). Results Totally an occupational group of 2480 brucellosis in 10 townships were investigated, and 604 sera were tested. Tweenty eight cases were infected, the infective rate was 4.64%(28/604), 15 cases were diagnosed as brucellosis patients, prevalence rate was 2.48% (15/604); cattle attendants had the highest infection rate of 6.45% (26/403). Conclusions Brucellosis epidemic in Shapotou District, Zhongwei City of Ningxia, and it is place related to vocation.

Objectives To investigate the associations of serum superoxide dismutase ( SOD) , malondialdehyde (MDA), and uric acid (UA) with blood pressure and the prevalence of hypertension in a Chinese population. Methods We measured serum superoxide dismutase (SOD), malondialdehyde (MDA) and uric acid (UA) in 506 randomly selected participants. For database management and statistical analysis, we used the SAS 9.13 Software. Results Compared with normotensive participants, hypertensive patients had significantly lower serum levels of superoxide dismutase activity ( =0.0002), and higher serum levels of malondialdehyde and uric acid ( <0.05) .Univariate analysis indicated that serum superoxide dismutase activity was negatively associated with blood pressure, and serum levels of uric acid (P 0.0001);while serum levels of malondialdehyde was positively associated with blood pressure, and serum levels of uric acid (P 0.05) . There was significant difference in systolic (Ptrend=0.02) and diastolic blood pressure (Ptrend=0.04) between three groups defined by tertiles of serum levels of superoxide dismutase, with a lower blood pressure in the highest tertile than in the lowest tertile. Furthermore, the systolic (Ptrend=0.01) and diastolic blood pressure (Ptrend=0.003) in the highest tertile, which defied by the serum levels of malondialdehyde, were significantly higher than those in the lowest tertile. Conclusions Hypertensive patients have a disturbed oxidative and anti-oxidative balance. Oxidative stress may play a role in the pathogenesis of hypertension in Chinese.

Objective To study the effect of listening to music with headphone or background music on pain reduction of patients undergoing colonoscopy. Methods 180 patients undergoing colonoscopy were randondy selected as the headphone group,background music group and the control group.The headphone group listened to relaxed music with headphone after measurement of vital signs.The background music group listened to relaxed music played by CD after measurement of vital signs.The control group did not listen to music.Pain degree was measured by pain intensity number scale during colonoscopy.Heart rate,blood pressure,saturation of blood oxygen and appraisal to the music by the former two groups were observed.Results Pain degree of the headphone group and the background music group was lower than that of the control group(P＜0.05).Pain degree of the headphone group was lower than that of the background music group with no significant difference(P＞0.05).Heart rate of the control group after colonoscopy was faster than before colonoscopy(P＜0.05).Conclusions Relaxed music can alleviate pain of patients undergoing colonoscopy.Listening to background music is a convenient and economical method for alleviation of pain without any side effects for patients undergoing colonoscopy.

Objective To study the effect of the evidence-based nursing in the treatment of acute myocardial infarction by the early thrombolytic therapy with urokinase.Method 138 patients with acute myocardial infarction were randomly divided into the observation group and control group in equal number: the former received the evidence-based nursing and the latter conventional nursing care.The two groups were compared in terms of recanalization rate,fatality and incidence of angina.Result The recanalization rate,fatality and incidence of angina in the observation group were all significantly lower than those in the control group(all P<0.05).Conclusion The evidence-based nursing used to nurse the acute myocardial infarction patient to be treated by is cared by early thrombolytic therapy with urokinase may prevent such complications as angina and reduce recanalization rate and fatality.

Objective:To observe the acoustic characteristics of glottal stops produced by patients with cleft palate.Methods:Twenty boys with cleft palate who were judged to be glottal-stop-producers and 20 normal peers were included. In their own way, all the children were asked to produce following sounds: /iu/ /pai/ /t′u/, /s/ /k′/ /p′u/ /t′u/, /tA/ /ku/ and /i/ /k′u/ /ti/ /suan/. Then the speech spectroanalysis and the comparison were performed by one investigator.Results:The acoustic characteristics of glottal stops changed a lot when they substitute or accompany with different oral stops, which included the emergence of spikes, changes of voice onset time, the formant transitions and spetroanalysis of spikes. Conclusion:The acoustic changes of glottal stops are complicated. Every single aspects of acoustic analysis should be considered in the identification of the acoustic characteristics.

Objective To investigate the influence of fast track surgery (FTS) on insulin resistance indexes and inflammatory reaction in colorectal cancer patients who received laparoscopic surgery.Methods A total of 62 patients were randomly divided into control group (n=31) or FTS group (n=31).The postoperative first flatus and defecation time, postoperative hospital stay, hospitalization expenditure, and postoperative complications were recorded.Insulin resistance indexes and inflammatory reaction were measured before operation (T0) as well as on the 1st, 3rd, and 7th postoperative days (T1, T3, and T7), including fasting blood-glucose (FBG), fasting insulin (FINS), serum level of interleukin-6 (IL-6), C-reactive protein (CRP), and tumor necrosis factor (TNF-α).The insulin resistance index (HOMA-IR) was calculated at the same time points.Results The postoperative time of flatus and defecation in the FTS group was significantly shorter than that in the control group (t=3.088, P=0.003;t=4.227, P=0.000), the postoperative hospital stay and total hospitalization expenditure were significantly lower in FTS group (t=3.937, P=0.000;t=3.478, P=0.003).No significant difference was found in the complication rate between the two groups (χ2=0.267,P=0.605).The inflammatory indexes and insulin resistance indexes in the two groups at T1 and T3 time points were higher than those at T0 time points (P<0.05).Compared to the FTS group at T1 and T3 time points, the levels of IL-6, CRP, TNF-α, FINS, and HOMA-IR in the control group increased more obviously (P<0.05), but the elevation blood FBG was not obvious (T1: t=0.870, P=0.388;T3: t=0.870, P=0.388).There were no significant differences in IL-6 and TNF-α in the two groups between the T7 and T0 time points (P>0.05), but the levels of CRP, FBG, FINS, and HOMA-IR at T7 time points were significantly higher than those at the T0 time points in the two groups (P<0.05).The levels of IL-6, TNF-α, FBG, FINS, and HOMA-IR at T7 had no significant differences between the two groups (P>0.05), but the level of CRP in the control group was significantly higher than that in the FTS group (t=-4.527, P=0.000).Conclusion FTS may promote the early recovery of gastrointestinal functions, reduce stress reactions and postoperative insulin levels, thus being conducive to fast rehabilitation in colorectal cancer patients with laparoscopic surgery.

Objective To study the role of different subtypes of PKC in the neurite outgrowth of primary cultured neurons from embryonic rat spinal cord. Methods We observed the expression and distribution of protein kinase C(?、?Ⅰ、?Ⅱ、? subtypes) by using immunohistrochemical technique in the neurite outgrowth of the primary cultured neurons from the embryonic rat spinal cord by means of computer image analysis system to measure the lengths of neurites and the areas of bodies. Results The areas of the cultured neuronal bodies increased and the neurites prolonged while the expression of the four subtypes of PKC gradually increased before 7div(day in vitro )during primary cultured neurons growth. The expression both in bodies and neurites of neurons decreased after 7div and the processes gradually shrinked and at the same time bubbles appeared inside the bodies. Relative assay demonstrated that ?Ⅱ subtype was most relative to the outgrowth of neurites( r =0 73, P