Objective If IL-2 and IFN-? are united,perhaps we may see a more significant antitumor effect.A fusion gene of IL-2 and IFN-? was designed and constructed by DNA engineering.Methods CEA promotor、IL-2cDNA and IFN-?cDNA were obtained by PCR and RT-PCR.SDS-PAGE was used to examine the results;pcDNA-IL-2、pcDNA-IFN-?、pCEA-IL-2/ IFN-?、pEGFP-IL-2/ IFN-? were transfected to CEA high expression cell line(Lovo)by use of liposome.The fusion protein expression was examined by fluorescence microscope and Western blotting.Results IL-2/ IFN-? fusion gene can be expressed in tumor cells.Its expression is more obvious in CEA high expressed cells(Lovo),and can be located in cell plasma.Conclusion The fusion gene can be expressed in plasma of CEA high expressed cell lines.It may have a new anti-tumor effects.

Objective: To investigate the effect of prenatal tobacco and alcohol exposure on stature and neural development of offspring. Methods: Female mice were exposed to tobacco smoke and alcohol drink from D6 of pregnancy to parturition. The early physical development, neurobehavioral and antioxidative functions were measured in offspring mice. Results: Compared with control, offspring subjected to prenatal exposure showed significant reduced body and organ weights as well as reduced gestational body weight gain. It also resulted in remarkable prolonged neonatal reflexes and reduced motive, coordinative and locomotor activities. Step-down and Y-maze tests revealed marked destroyed memory and learning ability. Futhermore, MDA in offspring brain cytosol and mitochondria increased significantly and the activities of SOD, GSH-Px, CAT and GST were decreased. Conclusion: Prenatal tobacco and alcohol exposures interfere with the early physical and neural development in mice, probably related to decreased antioxidative functions.

Textbooks are the base of teaching.The physiology textbooks used in medical education was compared between China and the United States.Guyton and Hall Textbook of Medical Physiology was chosen as the major representative of the United States.The overall framework, the selection and arrangement of the contents, the application of tables and pictures, and the relationship between basic medicine and clinical medicine were discussed.This paper may provide some information for remodeling of specialized course system and teaching contents of physiology, especially for improving the constructive quality of textbooks in the future.

Objective:To investigate the expression of latent membrane protein-1(LMP-1),NET-1,and vascular endothelial growth factor(VEGF)in non-keratin nasopharyngeal carcinoma(NK-NPC)and its clinical significance.Methods :Sixty biopsy specimens from pathologically-confirmed NK-NPC patients,who were treated in the Affilitated Hospital of Nantong University from May 1999 to May 2003,were included in the present study.Using immunohistochemical techniques (Envison two-step),we examined the expression of LMP-1,NET-1 and VEGF protein in the specimens.The relationship between their expression and elnicopathological parameters and the prognosis was anayzed.Ten specimens of chronic nasopharyngitis served as control.Results:(1)The positive rates of LMP-1,NET-1 and VEGF in NK-NPC were significantly higher than those in the chronic nasopharyngitis(P0.05).(3)NET-1 and VEGF expression in NK-NPC specimens were positively correlated with lymph node metastasis and distant metastasis(P

The lung is a major damage tissue in paraquat(PQ)-caused acute poisoned patients. Most deaths from acute PQ poisoning can be attributed to acute lung injury(ALI). Complement activa?tion product C5a causes inflammatory cells,such as neutrophils and macrophages,to accumulate into the lung and get activated. These activated inflammatory factors generate a large number of oxidants and inflammatory sequelae named cytokine storm to mediate ALI induced by PQ poison. The review focused on the role and mechanisms of complement C5a in PQ-induced ALI.

OBJECTIVE: To explore the anti-tumor and immunologic enhancement effects of Xiangling capsule.METHODS: The tumor-inhibition experiment was conducted in vivo in accordance with the screening criteria of anti-tumor drugs.Mice were treated with cyclophosphamide to induce hypoimmunity model.The effect of Xiangling capsule on mice was tested by determining leukocyte count,thymus index,spleen index,and the activity of NK cells.RESULTS: Xiangling capsules at different concentration showed inhibition on the growth of mice-transplanted tumor S180,H22 hepatomas and Lewis lung carcinoma with inhibition rates of 44.8%,52.2 %,and 43.3%,respectively(P

In the past decade, liquid chromatography-mass spectrometry has undergone rapid development in clinical laboratory medicine.Benefiting from the high sensitivity and specificity, as well as the capability of multi-component analysis in the single run, such technology is regarded as an excellent alternative to conventional immunoassays towards newborn screening for the genetic diseases, therapeutic drug monitoring and endocrine hormones detection.With the continuing progress in expertise-training, quality assurance and methodology standardization, it could be expected that liquid chromatography-mass spectrometry would become a dynamic and crucial part for routine clinical applications in the near future.

BACKGROUND:Peptide hydrogel has good plasticity, and it can fil the injured site very wel; therefore, to use this material as a scaffold is a feasible exploration in bone and cartilage tissue engineering. OBJECTIVE:To test the effect of bone marrow mesenchymal stem cels combined with injectable peptide hydrogel and chondrogenic factors for repair of articular cartilage defects in rabbits. METHODS:Bone marrow mesenchymal stem cels from rabbits were isolated and cultured. A ful-thickness bone-cartilage defect model, 5 mm in diameter and 3 mm in depth, was made on the left knee joint of rabbits, and the right knee joint of rabbits with no treatment was used as control after modeling. There were three experimental groups: self-assembling peptide hydrogel group, peptide hydrogel+chondrogenic factors group, and peptide hydrogel+chondrogenic factors+bone marrow mesenchymal stem cels group. Transforming growth factor β1, dexamethasone and insulin-like growth factor 1 were mixed as chondrogenic factors and added into self-assembling peptide hydrogel or bone marrow mesenchymal stem cels. Twelve weeks after treatment, animals were sacrificed for gross and histological observation, X-ray radiography, and histological evaluation using immunohistochemistry method. RESULTS AND CONCLUSION:At 12 weeks after treatment, the self-assembling peptide hydrogel group showed excelent results in the cartilage repair, and better achievements in safranin-O staining, colagen II immunostaining, and histological scores than the other groups (P < 0.05); the peptide hydrogel+chondrogenic factors group had better repair effects similar to the self-assembling peptide hydrogel group, but the expression of proteoglycans was higher than that in the control group (P < 0.01); there were poorer repair effects and more osteophytes in the peptide hydrogel+chondrogenic factors+bone marrow mesenchymal stem cels group than the the peptide hydrogel+ chondrogenic factors group. Experimental findings indicate that the self-assembling peptide hydrogel can repair cartilage defectsin situ and improve cartilage repair, which is expected to improve current repairing effects on cartilage defects.

Objective To observe the clinical efficacy of cluster needling at the anterior oblique line of vertex-temporal plus speech training in treating motor aphasia after cerebral infarction.Method Forty patients with motor aphasia after cerebral infarction were randomized into a treatment group and a control group, 20 in each group. Both groups were given basic neurological treatment. In addition, the treatment group was intervened by cluster needling at the anterior oblique line of vertex-temporal plus speech training, while the control group was by speech training alone. After 4-week treatments, the speech function and communication ability were observed and the clinical efficacies were compared.Result The total effective rate was 90.0% in the treatment group versus 80.0% in the control group, and the difference was statistically significant (P<0.05). After treatment, the speech function score and Chinese Functional Communication Profile (CFCP) score were changed significantly in both groups (P<0.01). There were significant differences in comparing the speech function and CFCP scores between the two groups (P<0.01,P<0.05).Conclusion Cluster needling at the anterior oblique line of vertex-temporal plus speech training is an effective approach in treating motor aphasia after cerebral infarction.

Dyslipidemia is one of the major risk factors for cardiovascular disease in elderly,but clinical practices in the management of elderly dyslipidemia are still not satisfactory.The cornerstone of treatment for elderly dyslipidemia consists of therapeutic lifestyle change and statins.Recent guidelines emphasize aggressive low density lipoprotein-cholesterol control with statins.However,intensive statin therapy may increase the risk of myopathy and diabetes.Therefore,it is important to consider risk benefit ratio in individual patients.The additional benefit of statins in combination with other hypolipidemic drugs on cardiovascular risk still needs verifying by further investigation.