In recent years, microRNAs (miRNAs) have been found to be one of the key factors of post transcriptional gene regulation, which are involved in occurrence and development of many diseases. Peritoneal dialysis (PD) has become an effective alternative treatment approaches for patients with end stage renal disease (ESRD). Peritoneal fibrosis is one of the most important factors leading patients to withdraw from long-term PD, hence restricts the application and development of PD. MicroRNAs are closely related to the development of peritoneal fibrosis. This article reviews the role of miRNAs in the pathogenesis and treatment of peritoneal fibrosis.

Objective In this study, we explored the mechanism of anti-bone resorption of RGD-insulin using osteoclast-like cells (OLCs) from giant cell tumor of bone as an in vitro model. Methods The function of bone resorption was observed and the staining for tartrate-resistant acid phosphatase (TRAPase) was identified. The carbonic anhydrase Ⅱ (CAⅡ) gene expression, apoptosis and the adhesion of OLC that treated by RGD-insulin and echistatin were determined by in situ hybridization, TUNEL staining and adhesion test, respectively. Results OLCs were TRAP positive and had bone resorptive function. After OLCs were treated with 10 -5, 10 -6, 10 -7mol/L RGD-insulin and echistatin respectively, the numbers of TRAP-positive multinucleated cells were (179?3), (238?11), (351?10) per well that treated with echistatin and (229?20), (222?21), (265?17) per well that treat with RGD-insulin.The expression of CAⅡ mRNA decreased, the number of OLCs apoptosis increased in a dose-dependent manner (P

Humans ; Molecular Epidemiology ; Respiratory Syncytial Virus Infections ; epidemiology ; virology ; Respiratory Syncytial Virus, Human ; genetics

Intracranial hypertension easily causes brain herniation,brainstem compression,and leads to death.Western medical treatment mainly chose medicines that can change the permeability of plasma,but with noticeable side effects.Chinese medicines had manifested therapeutic effects to inuacranial hypertension with multiple approaches and no obvious side effects.Besides Chinese medicines can also adjust the overall state of patients,which can not be replaced by western medicine.The treatment of intracranial hypertension with Chinese medicines will become an important research subject and have a broad outlook.

BACKGROUND:Tissue-engineered bone, as an emerging method, is used to repair bone defects, but it is difficult to be used widely because of no nutrient metabolism of implants. Pre-vascularized tissue-engineered bone research is performed for this limitation. Forward-looking and procedural vascular construction prior to artificial grafting is perspective to supply the nutrient metabolism of implants.
OBJECTIVE:To analyze the research results and developmental tendency of pre-vascularized bone engineering in vitro using a multilevel analysis.
METHOD:Literature search was performed in CNKI database for Chinese literatures and PubMed database for English literatures from 2000 to 2012. The key words included“tissue-engineered bone, vascularization, implant, osteoblasts, endothelial cel s, co-culture”in Chinese, and“bone engineering, endothelial cel s, osteoblast, implant, cel s co-culture”in English. A review addressing pre-vascularized tissue-engineered bone was completed based on retrieved literature classified according to bone physiological studies, in vitro experimental research and material research.
RESULTS AND CONCLUSION:Total y 60 articles were included in result analysis. Studied have documented that microvascular regeneration in normal bone tissue has an important role in osteogenesis regulation. Current bone tissue engineering research is to simulate this physiological process of vascular regeneration in the human body, and lots of in vitro studies are designed to complete this angiogenic process in bone tissue engineering. It is confirmed that in vitro pre-vascular work is used for bone tissue engineering, especial y for in vivo survival and ossification of tissue-engineered bone, pointing out the developmental direction for the clinical application of tissue-engineered bone.

Objective To explore the clinical efficacy of polyp of vocal cord. Methods The clinical data of 187 cases were retrospectively analyzed. Results According to the 187 cases, 164/187 cases were cured, 21 cases had obvious improvement, 2 cases have no effect, general effective rate is 98.9%. Conclusion Suspension laryngoscope were visual field widen and visual, every kind of surgical instruments binding equipments improve operation accuracy and reduce complication to acquire better clinical therapeutic effects.

Objective To evaluate the safety and efficacy of adrenal autotransplantation for the treatment of persistent Cushing's disease after transsphenoidal pituitary tumor resection. Methods Four patients were treated by adrenal autotransplantation with attached blood vessels after bilateral adrenalectomy for persistent Cushing's disease from April 1991 to March 2008 in our institute. The four patients were 3 females and 1 male. Their ages ranged from 14 to 36 years, with an average of 30 years. Right adrenalectomy was performed 1 to 3 months before the left adrenalectomy. 30 % ?50 % of the left hyperplastic adrenal was placed in the left inguinal region with the anastomosis of the inferior epigastric artery and the central adrenal vein, and between the left saphenous vein and the incised adrenal envelope with the adrenal middle artery inside. The hormone replacement dosage decreased gradually after operation. They were followed up for 1.0, 1. 5, 8. 0 and 10. 0 years. The patient's symptoms, adrenal hormone (serum cortisol, urinary free cortisol and adrenocorticotroph) levels and the steroid replacement dosages were recorded and analyzed. Results The symptom of the Cushing's disease disappeared completely after operation and there was no Nelson's syndrome except one patient with slight darken skin. Through 4 ways of imaging examination, operation, clinical presentation and endocrine examination, the 4 transplanted adrenals functioned well with less steroid replacement dosage needed after operation. The 4 transplanted adrenal glands functioned steadily 1 year after the auto-transplantation, and no hyperplasia was detected in the transplanted adrenals with the stimulus of high dosage adrenocorticotroph. Conclusions The long-term effects of adrenal autotransplantation with attached blood vessels after bilateral adrenalectomy is effective and safe. Adrenal autotransplantation can be a feasible option for the treatment of persistent Cushing's disease.

12E7 Antigen ; Adult ; Antigens, CD ; metabolism ; Calbindin 2 ; Cell Adhesion Molecules ; metabolism ; Female ; Follow-Up Studies ; Humans ; Hysterectomy ; methods ; Inhibins ; metabolism ; Middle Aged ; Ovarian Neoplasms ; metabolism ; pathology ; surgery ; Ovariectomy ; methods ; Retrospective Studies ; S100 Calcium Binding Protein G ; metabolism ; Sex Cord-Gonadal Stromal Tumors ; metabolism ; pathology ; surgery ; Young Adult

Target of the treatment for advanced pancreatic carcinoma is always palliative without prolongation of survival time almost, therefore the aims of treatment should be concentrated on improving the quality of life and alleviateing the disease-related symptoms. We do not call for the treatment and medicines that can incur obvious adverse reaction with limited effectiveness. Interventional therapy provides superexcellent short term efficacy with alleviating the disease-related symptoms significantly, and without severe side effects and thus coincides to the principle of the treatment for advanced pancreatic carcinoma. The surpassing advantages provide popular recommendation for this very kind of therapy in the recent years.

Objective To investigate the comparability of K + ,Na+ and Cl- measurement between Cobas8000 analyzer and AU640 analyzer .Methods According to the EP9‐A2 document of the National Committee for Clinical Laboratory Standards ,the precision of AU640 analyzer for the detection of K + ,Na+ and Cl- was evaluated ,and the correlation between the detected results of the two analyzers were also analyzed .Results AU640 analyzer were with high precision for the detection of K + ,Na+ and Cl- ,in‐tra‐assay and inter‐assay coefficient of variation of which were both less than 2% .The correlation coefficients of the two analyzers were higher than 0 .975 .Conclusion Cobas8000 analyzer and AU640 analyzer might be with fine comparability for the detection of K+ 、Na+ and Cl- ,both of which could provide accurate detected results .