Oligodeoxynucleotide containing unmethylated cytosine phosphate-guanosine motif(CpG ODN) may induce high expression of CD80, CD86, CD83, HLA I and HLAⅡ molecules on dendritic cells(DC) and stimulate DC to produce high level of IL-6, IL-12, TNF-? and IFN-?. CpG ODN is demonstrated in vivo to be a very potent adjuvant for Th1 cells, regulating Th0 cells to develop toward Th1 cells. Its role for DC is characteristics of CpG ODN sequence specificity and species specificity. CpG ODN is, at present, considered as a pathogen associated molecular pattern which binds its specific receptor,Toll-like receptor 9,then functions through TLR/IL-1R signaling pathway. It may represent a new therapeutic drug for broad applications in infectious disease, autoimmune disease, allergy and cancer therapy.

Objective To explore the relationship between subjective well-being (SWB) and behavioral inhibition/activation system (BIS/BAS) of older adults.Methods Using cluster sampling method and convenient random sampling method respectively for older adults in the nursing home and living home,we got 185 older subjects; then using BIS/BAS scale and SWB scale to test 185 older adults by oral questionnaire interviews face to face.Results (1) The SWB average score was 27.39±10.96,the BIS average score was 11.29±4.30,the BAS average score was 32.96±9.13.The SWB of older adults had a significant difference between older adults in the nursing (29.81±9.91) and living home (25.09± 11.47) (t=-2.69,P＜0.01).The negative experience of SWB had significant differences between older adults in the nursing (4.25±3.37) and living home (4.25±3.37) (t=3.58,P＜0.01),and also between males (4.25±3.37) and females (5.91±3.83) (t=-1.96,P＜0.05).The BAS fun seeking had a significant gender difference (t=2.05,P＜0.05),which was higher in males (12.60±3.48) than in females (11.40±3.67).(2) BAS was positively correlated with SWB (r=0.40,P＜0.01),and BIS was negatively correlated with SWB (r=-0.40,P＜0.01).(3) The BAS drive could forecast the 19％ variance of SWB.Conclusion BIS/BAS are correlated with SWB for older adults and the BAS drive is one of factors contributing to the SWB of older adults.

AIM: To study the effect of oligonucleotides containing unmethylated CpG motif (CpG ODN) on mouse bone marrow-derived dendritic cells (BMDCs) in anti-HcaF cytotoxicity. METHODS: BMDCs were stimulated by CpG ODN in combination with tumor antigen (TAg). The expression of CD80 on BMDC surface was analyzed by FCAS. Level of IL-12 (p70) in supernatants of BMDC culture was detected by ELISA. The proliferation of T cells was examined by MTT assay. Cytotoxicity of CTL induced by CpG ODN combining with TAg was detected by MTT assay. RESULTS: CpG ODN combining or not combining with TAg up-regulated the expression of CD80 on BMDC surface and stimulated BMDCs to produce a high level of IL-12. CpG ODN-activated BMDC promoted the proliferation of T cells. CTL induced by CpG ODN in combination with TAg appeared strong specific cytotoxicity on Hca-F cells. CONCLUSION: CpG ODN may effectively induce the functional maturation of mouse BMDC in vitro. CpG ODN in combination with TAg can enhance the anti-HcaF cytotoxicity of CTL. [

Objective To explore the impact of executive function intervention on the executive functioning of elderly patients with cerebral infarction,and to seek the best way to improve executive function in such patients.Methods Eighty elderly patients with cerebral infarction were randomly divided into an intervention group and a control group.A series of executive function interventions were administered to those in the intervention group with no intervention in the control group.The two groups were given the same conventional medication.The Behavioral Assessment of the Dysexecutive Syndrome (BADS) was taken before the intervention,then 3 months and 6 months after recruitment.Results In the intervention group the average score and the total standard score had increased significantly after 3 months except for the action program test and the modified six elements test.Both indicators were still significantly increased from the baseline afar 6 months except for the modified six elements test.The average score and the total standard score had increased significantly between 3 months and 6 months except on the key search test.Both the time determination test and the total standard score had statistically significant average differences.The control group showed no significant differences.After 3 months,the average scores and the total test standard scores in the intervention group were signifieantiy higher than those in control group except on the action program test.The rules conversion card test,the time determination test and the total standard score difference all showed statistically significant differences between the groups.After 6 months the intervention group's scores remained significantly higher than those of the control group except on the action program test.Conclusions Executive function interventions are effective in delaying the progress and improving the prognosis of executive dysfunction in elderly patients with cerebral infarction,and the therapy has persistent effects.

Objective To explore the value of contrast-enhanced FLAIR imaging in diagnosis of leptomeningeal metastasis.Methods 23 cases of leptomeningeal metastasis with definite primitive-tumour were collected.All the cases were confirmed by clinic and laborotary examination.Of the 23 cases,7 cases underwent contrast-enhanced T1WI only,16 case underwent contrast-enhanced T1WI and FLAIR.Two groups were analyzed comparatively in diagnostic accuracy and displaying lesions between images of two sequence.Results(1)2 cases were missed,and 1 case was unsure diagnosed in T1WI contrast group;16 cases were all diagnosed accurately in both T1WI and FLAIR contrast-enhanced group.(2)11 cases with fine linear enhancement and 7 cases with small nodular enhancement were more obviously on contrast-enhanced FLAIR than that on contrast T1WI,but the thick linear enhancement were equal on two sequence.4 cases with more lesions were displayed on contrast-enhanced FLAIR images.The signal intensity on contrast FLAIR images were higher than that on contrast T1WI images at the same lesions.Conclusion Contrast-enhanced FLAIR has important value in diagnosing leptomeningeal metastasis.

The aim of this study is to clarify whether edaravone postconditioning had protective effect against renal ischemia/reperfusion injury and to compare the protective effect between ischemic postconditioning and edaravone postconditioning. Rats were subjected to 45 min ischemia followed by 24 h reperfusion. The rats were randomly assigned to seven groups: a sham-operated control group, an ischemia/reperfusion group, an ischemic postconditioning group, a normal saline vehicle postconditioning group and an edaravone postconditioning (1, 3, and 6 mg x kg(-1)) group. Renal function was assessed by serum creatinine and BUN concentration, while histological damage of renal tissue was assessed with HE staining. MDA content and SOD activity of renal tissue were determined. TUNEL staining was performed to analyze the apoptosis of the tubular epithelial cells, the protein level of Bcl-2 and Bax in renal tissue was examined by Western blotting. Compared to the ischemia/reperfusion group, edaravone postconditioning significantly decreased serum creatinine and BUN concentration, and ameliorated histological damage of renal tissue. MDA was less after 24 h reperfusion in the edaravone postconditioning group than that in the ischemia/reperfusion group, consistent with an increase in SOD activity. In addition, edaravone postconditioning decreased TUNEL-positive cells and Bax expression, and increased Bcl-2 expression. Results detected in the edaravone postconditioning group showed no significant difference from the ischemic postconditioning group. Edaravone administered during the last 3 min of ischemia, prior to reperfusion induces a pharmacological postconditioning in vivo against renal ischemia/reperfusion injury in rats. This protection is similar to that observed with ischemic postconditioning.

Objective To study the expression of CD147 and collagen Ⅳ protein in gastric carcinoma.Methods Immunohistochemical assay was used to detect the expression of CD147 and collagen Ⅳ protein in 119 cases of gastric carcinoma, 20 cases of normal gastric tissues, and the clinical data was analyzed.Results In 119 cases of gastric carcinoma, the positive expression of CD147 protein was 83.2 %(99/119), the positive expression of collagen Ⅳ protein was 31.9 %(38/119). The expression of CD147 and collagen Ⅳprotein had relationship with metastasis of lymph nodes,depth of invasion and clinical phases (P ＜0.05). There was negative significant correlation between the expression of CD147 and collagen Ⅳ protein in gastric carcinoma (P＜0.01). Conclusion The expressions of CD147 and collagen Ⅳ protein may be used as one of the marks to study gastric carcinoma.

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Bone marrow transplantation was considered effective treatment for leukemias and other haematological diseases. Acute graft v-host disease (GVHD) is a major complication of allogeneic bone marrow transplantation. The T lymphocytes were specially depleted by anti-CD5, CD2, CD8, CD27 monoclonal antibody-ricin immunotoxins. Inhibition of the protein synthesis and T-cell functions in the target cells was observed in vitro. At 10 9mol / L of the immunotoxin, 3 logs (99.9%) of target cells were killed, but no cytotoxicity on nontarget cells. At the same concentration, the anti T cell immunotoxin had no any influence on the proliferating rate of CFU-GM and BFU E. None of the ten patients who received T cell depleted bone marrow developed grade III or IV acute GVHD.