2.Quality Control and Administration of Disinfectant in Manufacturing Laboratory
Li ZHU ; Junjia XING ; Wanyu FENG
Chinese Journal of Nosocomiology 2006;0(06):-
OBJECTIVE To prevent infection during the processes of dispensation and distribution of disinfectant. METHODS All the key processes were put under strictly control including purchase,preservation of disinfectant,quality of dispensing processes and administration of the disinfectant distribution. RESULTS Both effective ingredients and microorganism were coincided with the definite criterion. CONCLUSIONS Quality control and administration of disinfectant in manufacturing laboratory is important to guarantee the quality of disinfectant dispensation and to prevent from nosocomial infection as well.
3.Investigation of catheter-related thrombosis around the port in patients with breast cancer after chemotherapy
Li CHEN ; Feng LUO ; Lei XING ; Hongyuan LI ; Hong LIU
Journal of Shanghai Jiaotong University(Medical Science) 2017;37(4):514-517
Objective·To investigate the incidence, risk factors and treatment of the catheter-related thrombosis (CRT) in breast cancer patients after implantation of totally implantable venous access port (TIVAP) in chemotherapy. Methods·A total of 190 cases after implantation of TIVAP were investigated. Color Doppler ultrasound was used to monitor the neck blood vessels to find whether there was CRT before chemotherapy and before taking out the port. The incidence of CRT, occurrence time, risk factors and treatment efficacy were observed. Results·There were 112 (58.9%) cases with CRT and 108 (56.8%) patients with asymptomatic thrombosis, and only 4 cases had symptomatic thrombosis, the incidence of which was 2.1%. Most thrombosis developed on the 21th day after catheterization, and the patients over the age of 60, with clinical stage Ⅲ - Ⅳ and chemotherapy regimens TEC (docetaxel combined pirubicin and cyclophosphamide) were the risk factors for thrombosis. All the patients with asymptomatic thrombosis accepted anticoagulant treatment with low molecular heparin, earthworms enzyme or aspirin, respectively, but there was no significant difference in efficacy in the three groups (P=0.743). Conclusion·Port catheter related symptomatic thrombosis incidence is low but the incidence of symptomatic thrombosis is high in the breast cancer patients after chemotherapy. Age, tumor stage and TEC chemotherapy regimens are the risk factors for catheter-related thrombosis.
4.Application of Flow Cytometry in Viability Detection of Pichia pastoris Cells
An-Feng XIAO ; Xiang-Shan ZHOU ; Li ZHOU ; Yuan-Xing ZHANG ;
Microbiology 1992;0(06):-
Cell viability of Pichia pastoris was detected by flow cytometry (FCM) with two reagents fluorescein diacetate (FDA) and propidium iodide (PI). Compared with FDA/PI double-stained dot plots and PI single-stained dot plots,the latter could divide dead and living cells into two separate zones,and get the correct proportion. Then PI single-stained method was used to detect the change of cell viability in Pichia patoris fermentation. At glycerol batch and fed-batch phase,little dead cells were detected. At methanol fed-batch phase,cell viability decreased when cell weight increased,and was only 73.8% at 88 h.
5.Study on the relationship between altered expression of annexin A4 and endometrial receptivity during the implantation window in infertile patients with endometriosis
Yaling JIANG ; Bing LI ; Fuqi XING ; Fang WANG ; Jianhuai FENG
Chinese Journal of Obstetrics and Gynecology 2012;47(5):324-327
ObjectiveTo identify the differential expressed proteins,and to investigate the relationship between altered expression of annexin A4 during window of implantation [ WOI ( at day-6 after ovulatory day )] in infertile patients with endometriosis and endometrial receptivity.MethodsTwo-dimensional fluorescence differential in-gel electrophoresis (2D-DIGE) and matrix-assist laser desorption ionization-time of flight-mass spectrometry (MALDI-TOF-MS) were used to detect protein expression in endometrial WOI in 10 infertile cases with endometriosis as endometriosis group and 10 infertile cases with tubal factors as control group.The semi-quantitative validation of annexin A4 in the eutopic endometrial tissue during WOI was analyzed by western blot.Results By comparing protein profiles,there were 7 meaningful differential proteins during WOI in infertile patients with endometriosis.One protein with an isoelectric point of 5.84 and relative molecular weight of 36 100 were down regulated 348% in samples of endometriosis group.It was identified as annexin A4 by mass spectrometry.By western blot,relative intensity of annexin A4 in endometriosis group was 7.2 ±0.9,which was lower than 17.8 ± 2.6 in control group significantly (t =7.654,P =0.002 ).ConclusionLower expresssion of annexin A4 during WOI in infertile patients with endometriosis might be associated with the decrease of endometrial receptivity.
6.Observation on anricular-point plaster on children with simple obesity
Shuhua CUI ; Guangyao LI ; Yanjun XING ; Feng WANG
International Journal of Traditional Chinese Medicine 2010;32(1):66-67
Objective To observe effects of auricular-point plaster therapy on children simple obesity.Methods A total of 97 children of simple obesity were treated with auricular-point plaster.Body mass index was measured before and after the treatment.Therapeutic effects and positive changes of auricle were also observed.ResultsBody mass index decreased significantly(P<0.01)after the treatment as compared to the pre-treatment(t=3.142,P<0.01).Positive changes of auricle reduced.Auricular-point plaster showed therapeutic effects on obesity in different extents.Conclusion Auricular-point plaster was effective in treating children with simple obesity.
7.The clinical effect of video-assisted thoraeoscopic surgery (VATS) for chronic empyema
Dongshan WEI ; Hu LI ; Guoqing WANG ; Xing FENG
Chinese Journal of Primary Medicine and Pharmacy 2011;18(18):2489-2490
ObjectiveTo explore the clinical effect of video-assisted thoracoscopic surgery(VATS) for chronic empyema. Methods74 patients with chronic empyema were divided into experimental group and control group,control group was given decorticationoflung,and experimental group was given VATS. ResultsThe duration of chest tube drainage, hospitalization and complications in experimental group was significantly better than that in control group,it showed better clinical effect. ConclusionFor part of patients without the serious disease change of chronic empyema, if the surgery indication could be controlled strictly, the VATS was considerable.
8.Prospective randomized comparison of single pacemaker implantation using the subclavian vein puncture versus the cephalic vein cutdown
Boning XU ; Xing HUO ; Jiajian ZHAO ; Wenhui FENG ; Yonghua LI
Clinical Medicine of China 2013;(7):732-734
Objective To compare the value of pacemaker implantation using the subclavian vein (SCV) puncture and thecephalic vein(CV) cutdown approach.Methods One hundred and forty-six patients were randomized into SCV group (98 patients) and CV group (48 patients).We observed the success rate,surgery duration,fluoroscopy time and complications of the two groups.Results The SCV group had significantly higher success rate,shorter duration of surgery and fluoroscopy than the CV group (success rate:100% (98/98) vs.89% (43/48),x2 =10.5,P < 0.05 ; duration of surgery:(118 ± 35) min vs.(256 ± 75)min,t =12.12,P <0.01 ; duration of fluoroscopy:(15 ±5) min vs.(35 ± 10) min,t =13.08,P <0.01).Both groups had one case who developed compilations which were pocket hematoma and wire electrode dislocation respectively.The rate of complications was not significantly different between the two groups (P > 0.05).Conclusion The SCV approach should be considered a preferable access in pacemaker implantation.
9.Effect of nerve growth factor on adrenergic nerve in rals after myocardial infarction
Xiuqin NI ; Xing LI ; Jia FENG ; Linghui HAO ; Changwei JI
Clinical Medicine of China 2008;24(10):991-993
Objective To confirmthe protective effect of nerve growth factor (NGF)on cadiac adrenergie nerve in rats after acute myocardial infarction(AMI)and its mechanism.Methods 120 Wister rats were divided in-to sham-operated group.AMI group and NGF group.The samples were taken after6 h, d,4 d-7 d and 14 d sepa-lately.Immunohistochemistry method WaS used to show the distribution of adrenergic nerve fibers.The density of them were calculated by Medical Pathobgical Image Analysis Processing System.Results ①The densities of adrenergic nerve fibers in AMI group were obviously lower than that of sham-operated group greatly in 6 h and 2 d.4 d-7 d and14 d later.②The densities of adrenergic innervation in NGF group were obviously higher than that of AMI group 7 dand 14 d later.Conclusion NGF has protection effect 011 cardLac adrenergic nerve after AMI in the rat.
10.Risk Factors of Laryngeal Carcinoma:A Case-control Analysis
Shuai FENG ; Xing GUO ; Yan WANG ; Xiaotian LI
Journal of China Medical University 2010;(6):474-475,484
Objective To analyze the risk factors of laryngeal carcinoma.Methods The data of laryngeal carcinoma inpatients of our hospital from October 2005 to November 2007 were reviewed retrospectively by a case control analysis.Results The males who have smoking addiction,alcohol consumption,family history of malignant tumor and have more risks to suffer from laryngeal carcinoma.For The family history,OR is 2.01 in the single factor analysis,and in the multivariate analysis the OR is 2.27.Conclusion Gender,smoking,alcohol and family history of malignant tumor have great effect on the risk of laryngeal carcinoma.Family history of malignant tumor is an independent risk factor.