Objectives The relationship between homocysteine ( Hcy ) and lipid levels in renal transplant patients was analyzed to investigate the effect of hyperlipidemia on renal function and the importance of lipid monitoring in renal function after renal transplantation.Methods 492 patients outpatient follow-up of renal transplants from November 2014 to April 2015 in the Department of Urology and 221 healthy subjects from Shanghai First People′s Hospital were enrolled and their serum Hcy, Total cholesterol( TC) ,triglycerides( TG) , High density lipoprotein cholesterol( HDL-C) , Low density lipoprotein cholesterol( LDL-C ) were tested.All the subjects were divided into normal group and abnormal group according to the serum Hcy levels.At the same time serum cysteine protease inhibitor C(CysC) level was tested to estimate glomerular filtration rate(eGFR),grouping according to the principle of Chronic Kidney Disease( CKD)《Kidney Disease Outcomes Quality Initiative》( KDOQI) staging criteria.The co-relationship between serum TC, HDL-C, TG, LDL-C levels and the possibilities of high cardiovascular risk factors, and the relationship between of serum Hcy and lipid levers, kidney function were analyzed based on the standard of“2007 China Adult dyslipidemia prevention and treatment guidelines”.Statistical methods using U test、chi square analysis, correlation and multiple regression analysis.Results The serum TC(4.48 -5.85),TG (1.05-1.91),HDL-C(1.33-2.01),LDL-C(2.53-3.72) and Hcy(11.99-20.23) levels in the renal transplantation group were higher than the control group′s TC ( 4.02-5.33 ) , TG ( 0.81-1.74 ) , HDL-C (1.11-1.58),LDL-C(2.21-3.32), Hcy(8.9-12.4) levels.(P=0.00,0.00,0.00,0.00,P<0.01) In the renal transplantation group,serum Hcy levels was negatively correlated with eGFR and HDL-C levels (r=-0.565, -0.197, P<0.01), and was positively correlated with TG levels.(r=0.107, P<0.05) In renal transplantation group ,the serum levels of HDL-C was significant lower in Hcy-abnormal group(1.15-1.77)than the normal group(1.35 -1.97) (P=0.001,P<0.01),while the serum levels of LDL-C (2.51-3.93) was higher than the normal group(2.44 -3.69) (P=0.023,P<0.05).In the renal transplantation group,the count ratio of lipid risk factors in the Hcy-abnormal group (0 item 55.4%,1 item 23.5%,2 items 14.2%,3 items 6.5%,4 items 0.4%)was higher than normal group(0 item 69.8%,1 item 17.2%,2 items 9.1%,3 items 3.9%,4 items 0.0%) ( P=0.019,P<0.05).Ranked serum Hcy levels with cardiovascular risk factors, high-risk factors count ratio of eGFR 45-60 ml/min/1.73 m2 group was significant higher than eGFR 60-89 ml/min/1.73 m2 group in each level(P=0.018,P<0.05).Logistic regression analysis was carried out on the risk factors of serum TG, LDL-C, Hcy levels respectively, the results show that serum TG,LDL-C,Hcy levels and differences in renal function have statistical significance (P=0.00,0.00,0.00,P<0.01).Conclusions The serum TG,LDL-C and Hcy levels in the renal transplantation group were consistent increase and associated with kidney injury.The differences in TG, LDL-C, Hcy levels of renal transplant patients may cause differences in renal function after transplantation.Monitoring of serum lipids and Hcy levels is helpful for forecast the possibility of kidney injury.

Adolescent ; Adult ; Female ; Humans ; Male ; Niemann-Pick Diseases ; genetics ; Pedigree

Objective To investigate the significance of testing serum homocysteine for renal transplant patients .Methods 445 renal transplant patients from outpatient follow‐up(renal transplant group) and 100 healthy subjects(control group) were enrolled in the study ,whose serum homocysteine(Hcy) ,Cystatin C(CysC) ,creatinine(Cr) were tested .Then according to the eGFR(refered to the principle of NKF‐K/DOQI) patients of renal transplant group were divided into six subgroups .Serum levels of Hcy and Cr were compared among different groups ,and the relationship between serum Hcy concentration and anti‐rejection drugs were ana‐lyzed .Results In the transplant group ,concentrations of Hcy were obviously higher than that in control group(P<0 .05) .There was no significant difference in Hcy concentrations among renal transplants who had taken different anti‐rejection drugs(P>0 .05) . Concentrations of CysC and Cr were significantly associated with Hcy in renal transplant group(r=0 .481 ,0 .456 ,P<0 .05) .There was significant difference between eGFR≥90 subgroup and control group in CysC concentration(P<0 .05) .Conclusion Concen‐tration of Hcy in renal transplant group was obviously higher than that in control group .With the eGFR decreased ,Hcy increased gradually ,and in transplant group was associated with the concentration of CysC and Cr .There was significant difference between eGFR≥90 subgroup and control group in Hcy and CysC concentrations(P<0 .05) .The different anti‐rejection drugs had no effect on serum Hcy levels .

Objective To established a method for the content determination of ginsenoside Rg1 in Yushangling Capsules by HPLC. Methods HPLC was used with the C18 column. The mobile phase was acetonitrile-0.5% phosphoric acid (24∶76). The flow rate was 1.0 mL/min, the column temperature was 25 ℃, and the detection wave was set at 205 nm. Results The calibration curve was linear in the range of 0.05～0.80 ?g. The regression equation was Y =3629375.5X+2517.1, r =0.9998. The average recovery was 100.48% and RSD was 1.59%. Conclusion The method is simple, accurate and suitable for the content determination of ginsenoside Rg1 in Yushangling Capsules.

Objective To establish a method for simultaneous determination of icariin and naringin content in bushen huayu extract by HPLC. Methods Reverse-phase high performance liquid chromatography ( RP-HPLC ) separation was performed one C18 column (4.6 mmí250 mm,5 μm).The mobile phase was acetonitrile containing 0.2%H4PO3(pH=2.9). Gradient elution with a flow rate of 0. 8 mL·min-1 was applied to achieve the separation. The detection wavelength was set at 269 nm,and the column temperature was 30℃. Results Icariin had a good linear range from 0. 3-3. 0μg (r=0. 999 9),the recovery rate was 105. 6%,and RSD was 0. 95%. Naringin was linear within the range of 0. 12-1. 20 μg (r=0. 999 9). The recovery rate was 94. 0%and RSD was 0. 52%. Conclusion The method is simple,stable,accurate,and reproducible,which can be used as the quality control standard for bushen huayue extract.

Adolescent ; Adult ; Female ; Humans ; Male ; Methanol ; poisoning ; Occupational Diseases ; chemically induced ; diagnosis ; therapy ; Poisoning ; diagnosis ; therapy

Objective:This study aimed to identify the morphology of mast cells by using a modified toluidine blue staining scheme,so as to provide a powerful reference for the experimental basis research of mast cells.Methods:Bone marrow-derived mast cells were induced in vitro.After 4 weeks,the cells were collected,fixed,and stained.Mast cells were fixed at different temperature during different time.The optimum condition was determined by comparing the effects of toluidine blue staining.Results:Bone marrow cells were induced to differentiate into mast cells by SCF and IL-3 in vitro.When mast cells were stained with modified toluidine blue staining,the staining effect was better.Mast cells were round or oval and the cell membrane was complete and the cytoplasm was filled with a large number of purple particles.Conclusion:In this study,we successfully applied a modified toluidine blue staining method to mast cells cultured in vitro.The results showed that the condition at 37 ℃ full fixation with staining could reduce the degeneration of mast cells.This method was easy to operate with good stability.It was suitable for the morphological observation of mast cells cultured in vitro.

Objective To explore the relevance between lipoprotein(a) and atherosclerotic renal artery stenosis in adults.Methods Literature search was conducted in PubMed and EMBASE Database,using “atherosclerotic renal artery stenosis” as the search term as well as in Wanfang Database,China National Knowledge Infrastructure,and Cqvip Database,using “renal artery stenosis” and “lipoprotein” as the search terms,aiming to find case-control or cohort studies published before 2010.The qualities of all the literatures enrolled were evaluated using Newcastle-Ottawa scale and the data from which were analyzed by the Review Manager 5.0 software.Results Five eligible case-control studies (661 cases) entered the Meta analysis.The results showed that the lipoprotein(a) level was not significantly higher in the case group than that in the control group [ mean difference =0.0702 g/L,95％ CI ( - 0.0688,0.2092),P =0.32 ].Conclusion According to the existing studies,the relevance between lipoprotein(a) and atherosclerotic renal artery stenosis can not be established.

Objective To investigate the effects of the site for access to internal jugular vein (lateral versus anterior),lying position of patients (supine versus Trendelenburg),and head rotation (0°,20°,and maximum) during central venous catheterization on the location depth and cross-sectional area (CSA) of the right internal jugular vein (IJV).Methods Fifteen healthy volunteers were recruited in this prospective observational study from September 2008 to October 2008.Healthy volunteers were placed in flat supine position and 15°.Trendelenburg position separately.In each position,IJV were measured ultrasonographically from lateral site and anterior site with the head oriented at 0°,20°,and maximum rotation separately.Data of measured CSA and location depth of internal jugular vein in different positions were compared.Results The largest CSA (2.16 ±0.89) cm2 and location depth [(1.38 ± 0.43)cm] were occurred at the lateral approach in Trendelenburg position with head oriented at maximum rotation.The CSA in Trendelenburg position was larger than that in flat supine position.Only at the maximum head rotation,lateral approach got statistically larger CSA.The effects of head rotation varied with different degrees of rotation.Conclusions Site of approach,lying position and head rotation had noticeable effects on internal jugular vein cross-sectional area.Trendelenburg position increased the CSA of IJV.

ObjectiveTo evaluate the consistency of color Doppler ultrasonography (CDFI) and digital subtraction angiography (DSA) in diagnosis of carotid artery stenosis or occlusion. MethodsThe agreement and disagreement between CDFI and DSA examination results in 215 cases with unilateral carotid artery stenosis or occlusion were studied. By reviewing the CDFI sonogram and DSA dynamic image, the reason of discrepancy between two imaging modalities was analyzed.ResultsThe agreement rate was 90.7% (195 cases) between first CDFI scan and DSA, while disagreement rate was 9.3%(20 cases). Thus CDFI and DSA showed a good consistency (Kappa=0.7731,P<0.01). After reviewing the CDFI sonogram and DSA dynamic image, the discrepancy was attributed to treatment response in10 cases (4.7%), imaging principle difference in 3 cases (1.4%) , and inconsistent physician diagnoses in 7 cases (3.2%). ConclusionIn diagnosis of extracranial carotid artery stenosis or occlusion, the results of CDFI and DSA were highly consistent . In inconsistent cases, the examination results should be viewed under comprehensive and complementary analysis.