Objective To compare the bond strength between three different treatment factors.Methods Sixty extracted human premolars were randomly divided into 3 groups(n=20).Edgewise bracket was bonded on each tooth.The 24 h shear bond strength and tensile bond strength in 3 groups were tested respectively.The adhesive remnant index was recorded.Results The significantly higher shear bond strengths were observed in the polishing group with tental politure and the group that bonded with light-cured fluoride glass ionomer cements after polishing(Group 1∶5.973?1.519,Group 2∶9.017?3.278,Group 3∶7.862?2.437,P

By means of literature investigation and analysis, the papers related to exercise-induced Achilles tendon rupture and operative restoration were summarized, which provide evidences for further analysis. Results demonstrated that exercise-induced Achilles tendon rupture was associated with anatomic structure and local affection of Achilles tendon, as well as athletic techniques. Operative therapy was recommended when the Achilles tendon was completely ruptured. With the development of tissue engineering tendon, more and more biological patch materials have been used in the clinical repair of Achilles tendon due to the superiority on the prevention of postoperative adhesion and the substitution of Achilles tendon.

Objective To study the evaluation function of Tei index for right heart function of elderly patients with chronic cor pulmonale .Methods Choosed 98 cases of elderly patients suspected chronic cor pulmonale and 68 cases of the medical health elder-ly population from March 2009 to March 2012 in our hospital as group A and B ,ultrasonic inspection and Tei index were measured , ultrasonic inspection indicators contains ,right room transverse diameter ,the main pulmonary artery diameter ,wall thickness and be-fore in pulmonary artery systolic pressure .Results A group of 31 patients(31 .6% ) with a clear cor pulmonale ultrasonic signs ,42 patients(42 .9% ) did not see clearly cor pulmonale ultrasonic signs ,but there was three tricuspid valve and (or) pulmonary valve re-gurgitation ,27 cases(25 .5% ) patients were not seen clear cor pulmonale ultrasonic signs ,and did not see the tricuspid valve and pulmonary valve regurgitation ,three types of Tei index patients were significantly higher than the group B (P<0 .05) .Conclusion Tei index can reflect patients right heart function and can be used as a sensitive index evaluation .

Objective To investigate the distribution and the characteristics of drug resista-nce of coagulase negative Staphylococcus,and to provide the reference for properly choosi-ng antibiotic therapy of coagulase negative Staphylococcal infecs.Methods Contrasted 484 coagulase negative Staphylococcus(CNS) species isolated in hospital from 2001 to 2003 and 512 species from 2004 to 2007.Nosocomial CNS was identified and then drug resistance test was performed by K-B method,Nitrocefin method was utilized to detect β-lactamase.Clinical and Laboratory Standards Institute.Perfor-mance Standards forantimicro-bial susceptibility testing;Fifteenth information supplement.CLSI/NCCLS document M100-S17[J].Clinical and Laboratory Standards.Results The drug-resistant rate of.CNS to penicillin,erythromycin,oxacillin,amoxicillin/clavulanate,clindamycin,cepzolin,ceftriaxone and cefepime was high(>70%),whereas that to rifampin,nitrofurantoin,amikacin,tetracycline and trimetholvrim/sulfamethoxazole was low(<50%).No drug-resistant CNS strains to vancomyein were found.Conclusions,and no outstanding imparity from two groups.Conclusion The patterfi of resistance has changed greatly in the past 3 years.Clinical physicians should pay attention to properly choosing and using antibiotics.

Objective To investigate the expression of CD44V6 and Bcl-2 in cervical carcinoma and clinical significant in the progress of th is type of cancer. Methods A total of 153 cases of cervical carcinoma, normal cervical epithelium and cervical dysplasia were investigated for the expression of CD44V6 and Bcl-2 with immunohistochemistry SP method. Results ① The positive rate of CD44V6 was 69.44% in cervical carcinoma, 45.45% in cervical dysplasia and 8.33% in normal cervix epithelium ( P 0.05 )and pathological grades( P 0.05 ).Conclusion The overexpression of CD44V6 and Bcl-2 may be l inked to the pathogenesis, development and metastasis of cervical carcinoma. Det ermination of CD44V6 and Bcl-2 may be useful for properly estimating the progno sis of cervical carcinoma.

AIM: To optimize the preparation process of Fructus Schisandrae Chinensis dispensing granules METHODS:Schizandrin,schisantherin A,deoxyschizandrin,r-schizandrin were viewed as the indexes of judgement criteria,the influencing factors of extraction process as extract solvent,medical material particle diameter were studied and extraction parameters were optimized. RESULTS: Active components of dispensig granules can be extracted effectively by alcohol when medical materials were crushed.The best conditions were as follows:medical material were ground into 40 mesh,extracted two times by 70% alcohol,2 h per time. CONCLUSION: After being optimized,The process of dispensing granules is better than that of products on market.

Objective:To investigate the effect of GA on telomerase activity of breast cancer MDA-MB-435s cell.Methods:Human breast cancer cell line MDA-MB-435s was used in the study.Proliferation of MDA-MB-435s cells was measured with MTT assay.Cell cycle was analyzed by flow cytometry.The telomerase activity was measured by TRAP-ELISA assay.Results:GA inhibited the proliferation of MDA-MB-435s cells on a time-and dose-dependent manner.Aftertreatment of GA,MDA-MB-435s cells arrested at G2 /M phase.The telomerase activity of cells was decreasing compared with that of control cells(P

Objective To evaluate the clinical value of thinprep cytologic test(TCT)and the Bethesda system(TBS)for the diagnosis of early cervical diseases.Methods A total of 635 patients in our hospital had been examined by TCT and TSB from December 2005 to 2006.the abnormal cases which had been selected by TCT were detected by colposcopical pathological examination and Hybrid Capture Ⅱ HPV testing.Results We found 66(10.23%)abnormal smears out of 635 cases,in which atypical squamous cell of undetermined significance(ASCUS)37 cases(5.83%),low grade squamous intraepithelial lesion(LSIL)18cases(2.80%),high grade squamous intraepithelial lesion(HSIL)10 cases(1.57%),quamous cellcarcinoma 1case(0.16%).The rates of coordinance with pathological results of colposcopy and HCⅡ were 92.95% and 87.00% respectively.Conclusions TCT and TBS,sometimes combined with the colposcspy and HPV DNA test,may be a reliable method to screen and diagnose the precancerosis of cervix.

Objective To explore the effect of IL-23-stimulated synovial fibroblasts (FLS) in rheumatoid arthritis (RA) on the formation of human osteoclast-like cell. Methods Fibroblasts from patients with RA and osteoarthritis (OA) were stimulated with various concentrations of IL-23 (1, 5 and 10 ng/ml). After incubation for 72 hours, the level of receptor activator of nuclear factor kappa B ligand (RANKL) mRNA were, measured by using real time PCR. Human monocytes (MN) were isolated and cocultured with IL-23-stimulated RA or OA FLS, and subsequently stained for tartrate-resistant acid phosphatase (TRAP) activity. Osteoclast-like cell molecule markers were measured using real time PCR. Results Various concentrations of IL-23 upregulated RANKL expression in rheumatoid FLS, but failed in OA FLS. In the system of IL-23-stimulated RA FLS cocuhured with human MN, TRAP-positive osteoclast-like cell was enhanced and high level expressions of molecule markers were induced. Whereas in the system of non IL-23-stimulated RA FLS or IL-23-stimulated OA FLS cocuhured with human MN there was no TRAP-positive osteoclast-like cell formation. Conclusion The results suggest that IL-23 induced osteoclast-like cell formation is via RANKL expressions in rheumatoid FLS.

OBJECTIVE:To observe the efficacy and safety of lisinopril combined with losartan potassium in the treatment of chronic heart failure (CHF). METHODS:72 patients with CHF were randomly divided into observation group and control group. All patients were given salt restriction,diuretics and other conventional treatment. Based on the treatment,control group was orally treated with lisinopril tablets 10 mg,once a day,the dose was increased to 2 times after 1-2 weeks until the maximum dose of 40 mg,orally,once a day. Based on the treatment of control group,observation group was additionally treated with losartan potassi-um tablets 50 mg,orally,once a day. The efficacy was evaluated,and cardiothoracic ratio,LVEDD,LVESD,LVEF,HR,SBP,DBP and incidence of adverse reactions were observed after 2 months treatment. RESULTS:The total effective rate in observation group was significantly higher than control group(P<0.05). After treatment,cardiothoracic ratio,LVEDD,LVESD,HR,SBP and DBP in 2 groups were significantly lower than before,and observation group was lower than control group,LVEF was significantly high-er than before,observation group was higher than control group,there was significant difference(P<0.05). There was no signifi-cant difference in the incidence of adverse reactions(P>0.05). CONCLUSIONS:Based on the coventional treatment,lisinopril com-bined with losartan potassium has better efficacy than lisinopril in the treatment of CHF,with good safety.