AIM:To observe the histopathological changes of vascular calcificated rats following intervention with alendronate,further more,to verify the therapeutic action of alendronate on angiosteosis.METHODS:Thirty Sprague Dawley rats were divided into the normal,Vitamin D3 and alendronate groups by random number table after feeding for 1 week.Rats in the Vitamin D3 and alendronate groups were lavaged with 250 000 U/(kg ?d) Vitamin D3 or iso-capacity of sodium chloride at 0,24,and 48 hours after model preparation.From the fourth day,rats in the alendronate group were treated with 0.9 mg/(kg ?d) alendronate.All rats were sacrificed at the end of 6 weeks,removed aorta and aortic arch.The pathological change was analyzed by hematoxylin-eosin staining.In addition,calcium contents of blood vessel were measured.Von Kossa staining,Image-Pro Plus 6.0 image analysis software were used to determine the ratio of calcified plaque,and detected the content of blood fat.RESULTS:There had calcified plaque in the vessel wall of aortic arch in the alendronate group,but the number was obvious smaller than and the necrosis area was significantly lower than that of the Vitamin D3 group.The calcium contents of blood vessel,ratio of calcificated area,as well as the concentration of total cholesterol was decreased compared with the Vitamin D3 group(P

Objective To investigate the effects of mirtazapine ccombined with psychological treatment on depression of patients with epilepsy.Methods 102 patients with epilepsy and complicated with depression were divided into the control group and trial group with 51 cases in each group.The control group received conventional mirtazapine and the trial group received mirtazapine combined with psychological therapy.The course was six weeks.The follow-up was performed six months after treatment and all patients were evaluated with Hamilton Depression Scale(HAMD)and Clinical Global Impression(CGI-SI).The side effects were evaluated with Treatment Emergent Symptom Scale(TESS).Results After the treatment of six weeks,the HAMD and CGI-SI scores of patients of the two groups significantly increased(P<0.05)and the scores of the trial group increased more significantly than that of the cintrol group(P<0.05)six months later.Conclusion The effect of mirtazapine combined with psychological therapy on depression of patients with epilepsy is obvious.

Animals ; Embryo Culture Techniques ; Embryo, Mammalian ; Female ; Ganglia, Spinal ; cytology ; drug effects ; Glial Cell Line-Derived Neurotrophic Factor ; pharmacology ; Male ; Motor Neurons ; drug effects ; Neurons, Afferent ; drug effects ; Rats ; Rats, Sprague-Dawley

Objectives: To express and purify N-terminal peptide of human perforin(hPFP-N). 　Methods: The recombinant expressive plasmid pGEX-KG/hPFP-N was constructed and then introduced into a strain of E. coli BL21(DE3). Upon the induction of IPTG, GST/hPFP-N fusion protein was expressed. The expressed fusion protein was localized in inclusion bodies which could be solubilized by sonication after detergent lauroylsarcosine was added. The fusion protein was purified by affinity chromatography with glutathione agarose. After being cleaved by thrombin, GST and uncleaved fusion protein were removed by glutathione agarose beads once more, then purified recombinant rhPFP-N protein was obtained.　 Results and Conclusions: GST/hPFP-N fusion protein can be effectively expressed in E. coli and the protein hPFP-N was obtained after the purification process.

A 48-year-old female presented with a one-week history of painful and enlarged lymph nodes in the left neck.One-week systemic treatment with antibiotics resulted in no obvious improvement.Skin examination revealed palpable lymph nodes between the left lateral cervical papillae and clavicle,which appeared as a string of beads with a little mobility and obvious tenderness.The largest diameter of enlarged lymph nodes was about 2 cm.No enlarged lymph nodes were palpable in the other body sites.Histopathologically,histiocytes of various shapes,immunoblasts and plasmacytoid monocytes markedly proliferated with different degrees of karyorrhexis.Immunohistochemistry revealed that the lesions were positive for CD3,CDS,CD68,mouse macrophage inflammatory protein,and CD20.A diagnosis of histiocytic necrotizing lymphadenitis was made.

Objective To establish the quantitative method for detecting perforin mRNA. Methods The competitive templates were prepared by restriction endonuclease method.The quantitative RT-PCR assay was established and used to detecting the perforin mRNA level in peripheral blood of tumor patients and healthy adults.Results Average perforin mRNA level of six healthy adults is 0.51±0.40 pmol/ml.The perforin mRNA level of patients with digestive tumors are significantly lower than that of healthy adults(P<0.01).But compared with healthy adults,no significant difference is showed in patients with breast cancer or leukemia.Conclusion The decreased perforin mRNA level probably attributes to the high incidence of cancers.Except for perforin-mediated cytolysis,additional effective mechanism against tumor cells maybe exist.

Dendritic cells(DCs) are known as the most efficient antigen-presenting cells that are potent stimulators of both B and T cell immune responses.The biological characteristics and culture in vitro of DCs,preparation of DC vaccines,experimental and clinical studies in tumor immunotherapy by DC vaccines are reviewed in this article.

Objective To study the clinical and coronary characteristics of drug abuse women patients with acute myocardial in-farction .Methods Data of 70 women patients ,who were diagnosed with acute myocardial infarction(AMI)and underwent emergent coronary angiography in department of jurisdiction ,Guangdong Provincial Corps Hospital ,Chinese People′s Armed Police Force from January 2006 to July 2012 ,were analyzed .Results 66 of 70 were ST-segment elevated myocardial infarction ,4 were non-ST-segment elevated myocardial infarction ;67 of 70 were obstructive lesion ,underwent percutaneous coronary intervention .With the drug abuse time prolonging ,the combining risk factors increased ,the occurrence rate of AMI increased(P<0 .05) .Data of coronary angiography showed that the culprit vessels of most AMI patients with different drug abuse time were single vessel lesion ,which was significantly higher than the rate of three vessel and main stem .the most common culprit vessel was the left anterior descending artery ,followed by the right coronary artery and left circumflex artery lesion .three vessel and main stem lesion were short in pa-tients of drug abuse time <5 years ,with the prolonging of drug abuse time ,three vessel and main stem lesion increased .There was 1 death example in prison and 5 re-admission fore agnia during the follow-up .Conclusion Most AMI in drug abuse women patients with acute myocardial infarction is STEMI .Single vessel and obstructive lesion is the most common .The most common culprit ves-sel was the left anterior descending artery .Most female patients were complicated with congenital coronary malformation or autoim-mune disease .With the prolonging of drug abuse time and increasing of combining risk factors ,the rate of AMI increases .

OBJECTIVE:To investigate inhibitory effects of matrine on the proliferation of human bladder cancer BIU-87 cells and its mechanism. METHODS:The cell viability was detected by MTT assay and inhibitory rate of cell proliferation was calculat-ed after human bladder cancer BIU-87 cells were treated with 0(negative control),0.5,1.0,2.0 and 4.0 mg/ml matrine for 24,48 and 72 h,respectively. After treated with 0 (negative control),0.5,1.0,2.0 and 4.0 mg/ml matrine for 48 h,the cell cycle and apoptotic rate were detected by flow cytometry;the expression of Survivin,Caspase-3 and Caspase-7 protein were detected by Western blot assay. RESULTS:Compared with negative control,the proliferation of BIU-87 cells were significantly inhibited after incubated with 1.0-4.0 mg/ml matrine for 24,48 and 72 h(P<0.05 or P<0.01),and inhibitory rate of cell proliferation increased in concentration and time-dependant manner;after treated for 48 h,the percentage of G0/G1 phase cells and apoptotic rate in-creased,while the percentage of cells at S phase and G2/M phase were decreased (P<0.05 or P<0.01);the expression of Cas-pase-3 and Caspase-7 protein increased,while the expression of survivin protein decreased after incubated with 0.5-4.0 mg/ml ma-trine for 48 h. CONCLUSIONS:Matrine can inhibit the proliferation of human bladder cancer BIU-87 cells,block the cell cycle and induce apoptosis;its mechanism may be related to the expression regulation of Survivin,Caspase-3 and Caspase-7.

Purpose:To evaluate the clinical effect of late-course hyperfractionated (LCH) radiotherapy and conventionally fractionated (CF) radiotherapy for local advanced non-small cell lung cancer (NSCLC). Methods:From August 1995 to April 1998, 74 patients with inoperable stage Ⅲ NSCLC were randomized into two groups: CF and LCH. 37 patients in CF group received a fraction of 2 Gy daily, 5 days per week, to the total dose of 65～70 Gy /6.5～7 weeks. 37 patients in LCH group were treated with the same fractionation as CF group to the dose of 40 Gy, and then followed by LCH radiotherapy: 1.2Gy, twice daily, with the interval of 6 hours between fractions, to the total dose of 64 Gy/6 weeks. Results:The overall response rate in LCH group and CF group were 78.4%(29/37) and 62.2%(23/37) respectively, the difference was significant (P