1.Silicon nanowire biosensor in detecting tumor markers
Chinese Journal of Tissue Engineering Research 2013;(47):8248-8254
BACKGROUND:Tumor markers are of great significance in early diagnosis of cancer, screening of high risk
group with cancer, differential diagnosis between benign and malignant tumors, judgment of cancer progression, evaluation of cancer therapeutic effect and prediction of cancer recurrence and prognosis.
OBJECTIVE:To realize the importance of tumor marker for cancer early diagnosis by introducing the working principle and clinical application of silicon nanowires biosensor in detecting tumor markers.
METHODS:The first author searched PubMed, CNKI and Google Scholar Databases to retrieve relevant articles about tumor marker, working principle and application of silicon nanowires biosensor published from 2003 to 2013. RESULTS AND CONCLUSION:Silicon nanowires biosensors with high sensitivity and without secondary marks show a good application prospect in detecting tumor markers. They have been used to detect tumor markers for prostate cancer, breast cancer, tumor markers of colorectal cancer, and primary liver cancer, and achieved an ideal limit of detection. However, due to the influence of debye length, most of detections can only be performed in the standard solution. There is a lack of the serum data of cancer patients.
4.Cloning and Analyzing Biological Activity of A2 Gene in Q? Phage
Journal of Medical Research 2006;0(06):-
Objective To construct A2 gene expression vector in Q? phage by gene recombination technology, and then analyze its physiological activities. Methods Amplified A2 gene in Q? genome by PCR, cloned it into pBAD-24 expression vector to construct pBAD A2 recombinant plasmid. The recombinant plasmid was identified by restrictive enzymes digestion and DNA sequencing, then to be transfected into host cell JM109. After induced by Arabinose, the expression level of A2 was detected by SDS-PAGE. The growth curve of E.coli was obtained by phototurbidometry to test the bacteriolysis activity of pBADA2 in various host cells. Results After certified by PCR screening, DNA sequencing and restrictive enzymes digestion, the expression vector of pBADA2 was successfully constructed. The gene expression level is high in JM109 and related with Arabinose concentration, which reach its peak when Arabinose is 0.2%. OD660 value demonstrates that pBADA2 has the function of bacteriolysis, which could dissolve JM109、HB101 and 594 in E.coli rapidly, but not BE110. Conclusion The highly expressed vector pBADA2 was successfully constructed. The protein expressed has the ideal function of bacteriolysis. All of these provide theoretical and practical bases for developing new anti-bacteria drugs.
5.Effect of Human Growth Hormone on Diamine Oxidase and Endotoxemia in Systemic Inflammatory Response Syndrome
Chen WANG ; Tong WANG ; Xusheng LI ;
Chinese Journal of Bases and Clinics in General Surgery 2003;0(03):-
Objective To investigate the changes of diamine oxidase(DAO) and endotoxin(ET) during the treatment of systemic inflammatory response syndrome with human growth hormone and the relationship between human growth hormone and intestinal mucosal barrier injury. Methods One hundred and forty six patients with systemic inflammatory response syndrome were randomly divided into operative group and non operative group, which were again randomly divided into the study group and control group.Plasma concentration of DAO and ET were determined before the treatment and 1 week after the treatment.Results Plasma concentration of DAO and ET in study group decreased after treatment with significant difference ( P
6.Antileukemic mechanism of resveratrol in vitro and in mice bearing L1210-tumor.
Journal of Integrative Medicine 2008;6(12):1267-74
To elucidate the molecular mechanism of resveratrol against leukemia both in vitro and in vivo.
7.lntravitreal injection with ranibizumab combined with triamcinolone acetonide sub-Tenon injection for macular edema due to CRVO
Ting-Ting, LI ; Tong-Tong, NIU ; Hai-Lin, WANG
International Eye Science 2015;(1):98-100
Abstract?AlM: To evaluate the efficacy of intravitreal injection with Ranibizumab combined with sub-Tenon injection with Triamcinolone acetonide ( TA ) for macular edema ( ME) due to central retinal venous occlusions ( CRVO) .?METHODS:Forty-six patients (46 eyes) were diagnosed ischemic CRVO with significant macular edema by fundus fluorescence-angiography ( FFA ) and optical coherence tomography ( OCT ) . All the patients had panretinal photocoagulation ( PRP ) , a week after the four times therapies. Twenty-three patients ( 23 eyes ) in group A were randomly chosen to receive intravitreal injection with ranibizumab ( lVR ) , another 23 patients ( 23 eyes ) in group B to treat with both lVR and sub-Tenon injection with TA ( PSTT ) . There was no significant difference on macular edema and best corrected visual activity ( BCVA) between the two groups. The changes in BCVA and central macular thickness ( CMT) before and 1wk; 1, 3, 6mo after treatments were analyzed.?RESULTS: One week after the treatment: the BCVA increased while the CMT decreased compared with that of pretreatment in groups A and B (P<0. 05). BCVA and CMT changes between two groups were statistically significant differences (P<0. 05). One month and three months after the treatment:the BCVA increased while the CMT decreased compared with that of pretreatment in group A and B (P<0. 05), the difference was significant between two groups ( P< 0. 05 ). Six month after the treatment:the BCVA increased while the CMT decreased compared with that of pretreatment in groups A and B. Compare BCVA difference between the groups was statistically significant change (P<0. 05), CMT difference was not statistically significant (P>0. 05).?CONCLUSlON:Not only lVR can decrease ME caused by CRVO and increase the BCVA, but also lVR combined with PSTT can. But combined therapies can be more rapidly and have more positive effect on decreasing the ME and protecting the visual function.
8.Epigenetic inactivation of SPOCK2 in the malignant transformation of ovarian endometriosis
Fang REN ; Danbo WANG ; Tong LI
Chinese Journal of Obstetrics and Gynecology 2011;46(11):822-825
Objective To investigate epigenetic inactivation of SPOCK2 gene in the malignant transformation of ovarian endometriosis ( EM ) by comparing the methylation status and protein expression of SPOCK2 gene in the malignant tissues,ectopic endometria and the eutopic endometria of endometriosis.Methods From Jan.2005 to Jan.2011,22 paraffin-embedded specimens diagnosed as malignant transformation of ovarian endometriosis ( EAOC ) including 11 cases with ovarian endometrioid carcinoma,8 cases with clear cell carcinoma,2 cases with serous cystadenocarcinoma and 1 case with mucous cystoadenocarcinoma matched with 22 cases with ovarian endometriosis and 16 cases with normal endometrium form cervical intraepithelial neoplasia(CIN) patients as controls in Department of Obstetrics and Gynecology of Shengjing Hospital.Twenty-two malignant tissues,15 ectopic endometria and 10 eutopic endometria were captured by microdissection in EAOC group; 22 eetopic endometria and 17 eutopic endometria were captured in EM group; 22 endometrium were captured in the NE group.The methylation statue of SPOCK2 was determined by combined bisulfite restriction analysis,and the protein expression of SPOCK2 was evaluated by immunohistochemistry.Results ( 1 ) Methylation of SPOCK2:in the EAOC group,the frequency of SPOCK2 hypermethylation in malignant tissue was 45% (10/22),which was significantly higher than 1/15 in the ectopic endometrium (P<0.05).There was no statistical difference of the frequency of SPOCK2 hypermethylation in ectopic endometrium in the EAOC group(1/15) and EM group (5%,1/22) (P>0.05).(2) SPOCK2 protein:the loss rate of SPOCK2 was 44% (11/22) in malignant tissue in EAOC group,which were significantly higher than 2/15 of in ectopic endometrium of EAOC (P < 0.05).However,there was no remarkable difference in loss rate of SPOCK2 protein between ectopic endeometrium of EAOC and endometrium of EM [ 2/15 vs.5% ( 1/22 ),P > 0.05 ].No significantly difference in loss rate of SPOCK2 in eutopic endometrium was observed among three groups ( P > 0.05 ).(3) The abnormal methylation of SPOCK2 could lead to loss expression of protein ( P < 0.05 ).Conclusion Epigenetic inactivation of SPOCK2 gene is involved in the malignant transformation of ovarian endometriosis.
9.Anti-androgenic Activity of Cypermethrin and Fenpropathrin in Hershberger Assays
Junwang TONG ; Ying WANG ; Jun LI
Journal of Environment and Health 2007;0(07):-
Objective To observe the anti-androgenic activity of two pyrethroids (cypermethrin and fenpropathrin) in vivo. Methods SD rats aged 6 weeks were castrated ,and then housed for one week. The rats were randomly divided into groups, six in each: negative control group,cypermethrin and fenpropathrin group, positive control group. The rats were subcutaneously injected with testosterone propionate (TP) 100 ?g(/kg?d)for 7 consecutive days. Peanut oil, cypermethrin [ 45, 90, 180 mg/(kg?d)], fenpropathrin[10, 20, 40 mg/ (kg?d) ], flutamide[ 20 mg(/kg?d) ] were subcutaneously injected for 7 consecutive days at the same time. The sex accessory glands and serum hormone were detected. Results Compared with the negative group, no significant differences were observed in the sex accessory glands/tissues (seminal vesicles with coagulating glands, dorsolateral prostate, the ventral prostate and levator ani muscles) and the levels of serum hormones of the pyrethroids groups. Compared with the negative group, the masses of all the sex accessory glands/tissues decreased significantly in the positive group . Compared with the negative group, the level of LH increased significantly in the positive group. Conclusion No obvious anti-androgenic effects induced by cypermethrin and fenpropathrin in castrated SD rats has been observed.
10.Benzene Series Compounds Pollution inside Passenger Cars in Tangshan
Junwang TONG ; Ying WANG ; Jun LI
Journal of Environment and Health 1993;0(03):-
Objective To understand the situation of benzene series compounds pollution inside some Passenger cars in Tangshan and provide data for the formulation of corresponding countermeasures for the prevention and control of the pollution. Methods One hundred and twenty-two cars used for different periods of time were selected from March, 2009 to May, 2009 and the concentrations of benzene,toluene and xylene were determined.At the same time the basic situation (car type, date of manufacture) was investigated with questionnaire. Results The disqualified rate of benzene, toluene, xylene were 14.8%(18/122), 26.2%(32/122) and 20.5%(25/122) respectively. Eighty-one cars did not meet the standard, accounting for 66.4 %(81/122). The median of benzene, toluene, xylene in the cars used within six months were 0.086, 0.900, 0.400 mg/m3 respectively. The exceeded rate of benzene, toluene, xylene inside the cars which were used for not more than six months were 28.6%(10/35), 57.1%(20/35), 60.0%(21/35) respectively. The concentrations of benzene series compounds decreased gradually later on. The concentrations of benzene inside the cars with adsorbent were lower, the concentrations of toluene inside the highgrade cars were higher and the concentrations of xylene inside the cars with leather seats was higher. Conclusion Benzene, toluene, xylene pollution inside cars has been showed in this investigation in Tangshan. The concentrations of benzene series compounds are associated with using time, adsorbent, car grade and the seat material.