This paper reports a new solid self-emulsifying drug delivery system--self-emulsifying microsphere prepared by membrane emulsification technology with the hydrophobic berberine hydrochloride as a model drug. Solubility test and pseudo-ternary phase diagram were employed to select the optimal prescription of liquid self-emulsifying drug delivery system. The self-emulsifying microsphere was prepared by membrane emulsification technology with the solid carrier in a gel formed by sodium alginate and calcium chloride. The results showed that the optimal prescription of liquid self-emulsifying drug delivery system was Capmul MCM/Cremophor RH40/Labrasol/1-2propanediol = 20 : 32 : 32 : 16. The solid self-emulsifying microsphere had average diameter of 10.92 microm, encapsulation efficiency of 32.57% and the droplet size of reconstituted micromulsion of 156.5 nm. Berberine hydrochloride was dispersed in microsphere in non-crystalline form. In vitro release of the self-emulsifying microsphere showed pH response characteristics. These results indicated that the self-emulsifying microsphere prepared by membrane emulsification technology might become a new dosage form for poorly water soluble drugs.

AIM: To compare the visual effects of optimized aspheric transition zone Laser in situ keratomileusis (OAT z-LASIK )versus conventional Laser in situ keratomileusis (con. LASIK).METHODS: In a prospective study 44 patients (88 eyes)underwent OAT z -LASIK and 50 patients (92 eyes) received conventional LASIK surgery. Visual acuity,refractions、contrast sensitivity (CS),glare sensitivity (GS), and increment of Q-value after surgery were studied.RESULTS: All of operations on 94 cases were successful without severe complication after 3 months' follow-up. There was no significant difference in term of postoperative refractions between the two groups (t-test,P＞0.05). The uncorrected visual acuity (UCVA), CS, GS, and increment of Q-value of OAT z group were significantly better compared to the tradition group 3 months after the surgery (P＜0.05).CONCLUSION: OAT z - LASIK is a more effective procedure to improve visual quality compared to conventional LASIK.

Objective To compare the clinical efficacy of esomeprazole or omeprazole triple therapies on gastric ulcer patients complicated with type 2 diabetes mellitus.Methods Seventy-five patients with Hprelated gastric ulcer and type 2 diabetes mellitus were enrolled and were randomly divided into group A(n=39)and group B (n =36).Patients in group A were treated with esomeprazole,amoxicillin and clavulanate potassium dispersible tablets,Clindamycin.Patients in group B were treated with omeprasole,amoxicillin and clavulanate potassium dispersible tablets,Clindamycin.Patients in both groups were treated with hypoglycemic therapy.The efficacy and the Hp eradicate rate of the two groups were observed and compared.Results The cure effect in group A was 97.44％(38/39),much better than that of group B(80.56％(29/36);x2=5.598,P=0.025).The Hp eradicate rate in group A was 92.31％,also much better than that of group B(75.00％;x2 =4.172,P =0.041).Conclusion The clinical egicacy and the Hp eradicate rate of esomeprazole triple therapies on gastric ulcer patients complicated with type 2 diabetes mellitus is better than that of omeprazole triple therapies.Esomeprazole triple therapies and it is worthy of being clinically promoted.

AIM: Firstly to evaluate the changes of corneal sensation and its effect to dry eye syndrome after laser in situ keratomileusis (LASIK); secondly to evaluate the relation between the changes of corneal sensation and the laser ablation depth. ·METHODS: 30 patients (60 eyes) had bilateral LASIK with a superior-hinged flap. Every patient underwent preoperative and postoperative (after one week, one month and three months) evaluations, including corneal sensation, Schirmer's basic tear secretion test, tear breakup time (TBUT), corneal fluorescein stainting, and a questionnaire evaluating dry eye obtained from each patient.·RESULTS: Compared with preoperative level, after one week and one month, the difference of corneal sensation was very significant (P＜ 0.01); after three months, there was no statistical difference (P ＞ 0.05). After one week, there was no statistical difference (P ＞ 0.05) in the subjective dry eye symptoms, after one month, the difference was significant(P=0.025 ＜0.05) and after three months, the difference was very significant (P=0.001 ＜ 0.01). Compared with preoperative level, the basic tear secretion decreased significantly after one week and one month(P＜ 0.01), it did not return to the baseline level after three months (P ＜ 0.01). Tear breakup time decreased significantly after the surgery, and after three months, there was still statistical difference compared with preoperative level (P ＜ 0.01); with cornea fluorescence staining, we found corneal epithelial defect was most serious after one month after LASIK, and did not return to the baseline level after three months (P ＜ 0.01). Linear regression analysis and relevant were used to evaluate the relation between the changes of corneal sensation and the laser ablation depth (r=0.798, P ＜ 0.01). ·CONCLUSION: The central corneal sesation decreased noticeable after LASIK, and it recovered three months postoperatively, but the indicators of dry eye syndrome did not return to their preoperative levels following it. We have found that the decreasation of corneal sensation had positive correlation with the laser ablation depth.

OBJECTIVEThrough a simulation of interstitial fluid pressure (IFP), we developed an in vitro model to explore the change law of biological characteristics of adenoid cystic carcinoma (ACC) under different IFP.

METHODSA pressure cooker was refitted into a controllable pressure device. Cultured ACC-2 cells were subdivided into different groups, namely, negative control (untreated ACC-2) and experimental group (stressed for 3, 6, 12, 24 h under pressure of 7.551, 7.649, 7.747 kPa). CCK-8 and immunofluorescence of Ki67 were used to reflect proliferation ability. Transwell chamber assay was performed to observe the invasion ability of cells.

RESULTSThe proliferation ability was positively correlated with treatment time, and the peak value was obtained after the cells were subjected to 7.649 kPa of stress for 24 h. The invasion ability of ACC-2 cells was upregulated under stress.

CONCLUSIONWe successfully developed an in vitro model of IFP and found that high IFP can stimulate cell proliferation ability and upregulate invasion ability.

Low-frequency repetitive transcranial magnetic stimulation (rTMS) is a non-invasive brain stimulation method that has been used in the treatments of stroke patients. Our purpose was to investigate the effects of low-frequency rTMS on the hand motor function recovery for stroke patients. After searching from electronic database from January 1990 to April 2012, we collected randomized controlled trials explored the low-frequency rTMS effects on the hand motor function rehabilitation. A total of 7 comparisons (N = 216) from 5 articles were included in this study. We summarized the effect size by calculating the standardized mean difference (SMD). Summary of effect size (SES) indicated positive effects of low-frequency rTMS on the finger motor ability (SMD = 0.58) and hand function (SMD = -0.98). Few adverse effects occurred. The evidence showed that low-frequency rTMS could improve the paretic hand recovery after stroke.
Hand ; physiopathology ; Humans ; Recovery of Function ; Stroke ; therapy ; Transcranial Magnetic Stimulation

Humans ; Occupational Diseases ; diagnosis ; Occupational Health ; Occupational Health Services ; United Kingdom

Amyloidosis ; complications ; pathology ; Heart Diseases ; etiology ; pathology ; Humans ; Male ; Middle Aged

Objective To explore the effect of isofluranee on receptor for advanced glycation end products (RAGE) expression of hippocampus and learning and memory function in rats. Methods 24-month Sprague-Dawley male rats (n=45) and 4-month Sprague-Dawley male rats (n=45) were randomly divided into six groups with 15 rats each group. Group C1 (aged control group),group C2(adult control group) breath 30% oxygen and air mixed gas; Group S1(single inhalation of isoflurane aged group),Group S2(single inhalation of isoflurane adult group)were anesthetized with 1.5% isoflurane,breath 30% oxygen and air mixed gas for 2h;Group R1(Repeated inhalation of isoflurane aged group), group R2(Repeated inhalation of isoflurane adult group) were anesthetized with 1.5% isoflurane,breath 30% oxygen and air mixed gas 2h a day for three days. Eight rats randomly selected from each group were killed and their hippocampus were immediately isolated for detection of RAGE expression by immunohistochemistry and RT-PCR after accomplished treatment 24h. The remained rats' learning and memory function were assessed using Morris water-maze test. Results The results of Morris water-maze test showed that the times of acerossing the original platform and the time consumption of staying the original platform quadrant was shorter in group S1 and group R1,but the escape latency was longer than group C1(escape latency C1 (9.42± 2.63)s,S1(13.20±3.85)s,R1(17.20±3.44)s, F=12.773, P<0.05). In the group R2,the escape latency was longer but the times of accrossing the original platform and the time consumption of staying the original plat-form quadrant was shorter than group C2 (times of accrossing the original platform C1(7.30±2.40), S1(3.90± 2.42),R1(3.44±2.40), F=7.448, P<0.01).To contrast with the group C2,there were no significant differ-enees in spatial probe test in the group S2(P>0.05). The levels of mRNA and protein of RAGE in hippocampus was significantly higher in group S1 and group R1 than group C1(RAGE mRNA expression C1(0.11±0.02),S1 (0.56±0.09), R1(0.73±0.14), F=179.447, P<0.01). To contrast with the group C2, there were no differ-ences found in the levels of mRNA and protein of RAGE in group S1(P>0.05), but it was higher in the group R2 (RAGE mRNA express C2(0.22±0.04), R2 (0.41±0.08), F=40. 209, P < 0. 01). Conclusion Isoflurane can reduce learning and memory function in both aged and adult rats, but aged rats are particularly significant im-pacted. This effect may be induced by the increase of RAGE expression in hippocampas.

Objective To investigate the effects of inhalation of different concentrations of isoflurane on the expression of cytochrome c ( Cyt c) in hippocampus in aged rats.Methods Sixty-three aged male SD rats (20 months) weighing 500-600 g were randomly divided into 3 groups(n=21each):control group inhaling 30%O2 for 2h (group C) and 2 isoflurane groups anesthetized with 0.75 % and 1.5 % isoflurane in 30 % O2 for 2 h respectively (group Ⅰ1 and Ⅰ2 ).Arterial blood samples were obtained from 5 rats at 30 min, 1 and 2 h of anesthesia for blood gas analysis. Eight animals were killed at 24 h after anesthesia in each group.Their hippocampi were immediately removed for determination of Gyt c expression by immuno-histochemistry and Western blot analysis.Cognitive function was assessed by Morris water maze test the day before experiment and once a day for 6 consecutive days starting from the 1st postoperative day.Results The Cyt c expression in hippocampus was significantly increased in Ⅰ1 and Ⅰ2 groups in a concentration-dependent manner as compared with group C.The escape latency was significantly prolonged and the frequency of crossing the original platform and the duration of staying at the original platform quadrant were decreased in group Ⅰ1 and Ⅰ2 compared with group C.Conclusion Inhalation of isoflurane anesthesia can decrease cognitive function through up-regulating the Gyt c expression in hippocampus in aged rats.