AIM: To investigate the cellular biological effects of matrine on K562 and K562/Vin cells and discuss the anticancer mechanism of matrine. METHODS: MTT assay was used to detect the IC_ 50 of matrine on these two cell lines and the reversal effect of matrine on K562/Vin cell's resistance to vincristine. In addition, the growth curve of cells was drawed. The p-glycoprotein (P-gp) expression was determined by immunohistochemistry analysis. The morphological changes of cells under light microscopy and the structural changes under transmission electron microscope were observed. RT-PCR assay was used to detect the hTERT-mRNA expression. RESULTS: The IC_ 50 of matrine was 3.4, 4.6 mmol?L -1 for K562 and K562/Vin cells, respectively. Matrine (4.0 mmol?L -1) inhibited the growth of K562, K562/Vin cells, 2.0 mmol?L -1 matrine inhibited expression of P-gp and with 492.4 reversal index. Matrine killed K562 cells by inducing the apoptosis and the same effect on K562/Vin cells was also observed. The hTERT-mRNA expression of K562 cells were also inhibited by matrine. CONCLUSIONS: Matrine enhanced the cytotoxicity of vincristine in K562/Vin cells, induced the apoptosis of K562 and K562/Vin cells, also inhibited the hTERT-mRNA expression in K562 cells. It shows that matrine would be an effective anticancer medicine.

ObjectiveTO study the mechanism of xingnao kaiqiao acupuncture for brain occlusion. Methodusing one-side middle cerebral artery occlusion(MCAo)rats by thermoregulation as focal cerebral ischemis experimental animal. The dynamic changes of c-fosprotein in sichemic region of brain tissue were observed by imnunohistochemitry method. Meanwhile the effect of acupuncture was stud-ied. ResultThe outcome of xingnao kaiqiao group was better than that of rourine group. Acupuncture could enhance the expression of c-fosprotein in all stages of ischemia, promote the stress ability of neuron produce adaptive changes to brain sichemia and improve recovery of braintissue. ConclusionThe dffect of xingnao kaiqiao acupuncture was better than that of routine acupuncture.

Objective The recurrence and metastasis of malignant tumor could affect the survival of patients. Early detection of cancer cells in peripheral blood and effective therapy promptly are of clinical importance. The present study was to investigate the carcinoembryonic antigen (CEA) mRNA expression and serum CEA level in blood from patients with colorectal cancer and to evaluate its clinical significance. Methods Peripheral blood samples were collected from 35 eligible patients with colorectal cancer and 9 post operative patients. CEA mRNA was detected by reverse transcriptase polymerase chain reaction (RT PCR) and serum CEA was detected by time resolved fluorometric methods. Results The positive rate of CEA mRNA in blood samples of 35 patients with colorectal cancer was 45.7%, and 2.7% in healthy volunteers. Two of the nine post operative patients (22.2%) showed CEA mRNA positive. The positive rate increases with the pathological staging and showed no significant relation with the malignant extent of cell differentiation. Serum CEA mRNA positive rates in patients with CEA positivity (72.2%) were significantly higher than those in patients with negative CEA mRNA expression (42.3%).Conclusion The expression of CEA mRNA in peripheral blood cells correlates with the pathological staging of colorectal cancer and it could be of potential use to monitor the micrometastases of tumor. Long term follow up is needed to evaluate its clinical significance.

Adolescent ; Antigens, CD20 ; metabolism ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Male ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; diagnosis ; metabolism ; pathology ; Prognosis

This paper analyzed the system,methods,content,scope and standards of last two competition in order to further improve the clinical skills competition for national medical students in China.According to the characteristics of medical education,the paper proposed several opinions and suggestions as follows:repechage should be cancelled in competition system and objective structured clinical examination should be used in competition; the content,scope and criteria of the competition should take the actual ability of the medical students at graduation into consideration.

Objective To discuss the advantages and disadvantages of the mammography and ultrasound examination for breast cancer diagnosis.Methods Totally 84 breast cancer patients enrolled in our study.All of the patients underwent mammography before surgery,and 69 of them received both mammography and ultrasound examination.The data were studied retrospectively.Results 84 foci were found totally on mammography and were all diagnosed above ACR BIRAD grade 4 by X-ray.Two of the 13 cases smaller than 2 cm in diameter were diagnosed as grade 3 by ultrasound.Eleven cases with microcalcification were diagnosed as grade 2（1case）and 3（1case）by ultrasound.Only 1 of the 6 cases shown as distortion was diagnosed as 5 by ultrasound.Conclusion X-ray has the superiority to ultrasound for distortion and microcalcification.For the mass smaller than 2 cm in diameter,both of X-ray and ultrasound may underestimate.

Objective To quantitatively evaluate the difference in global longitudinal strain(GLS)and global circumferential strain (GCS)of three-layers of cardium between diabetes mellitus (DM)patients using layer-specific speckle-tracking imaging.Methods Fifty-seven DM patients and sixty matched controls were enrolled in the study.GLS and GCS of three-layer cardium were assessed using layer-specific speckle-tracking imaging,available by GE Vivid E9 and EchoPac workstation.Results GLS of three-layer cardium in DM patients were significantly lower than those in normal controls (all P <0.05).GCS of endo-and mid-cardium were significantly lower in DM patients than in normal controls (P < 0.05 ).There was no statistical difference of GCS of epi-cardium between the two groups (P > 0.05 ).GLS and GCS of three-layer cardium was highest in endo-cardium and lowest in epi-cardium.Conclusions GLS of three-layer cardium may be a sensitive indicator of early left ventricular systolic dysfunction in DM patients with normal ejection fraction.

Objective To investigate longitudinal systolic function of transplanted heart using two dimensional speckle tracking echocardiography and tissue Doppler imaging.Methods 56 consecutive patients with heart transplant were recruited,according to myocardial biopsy and 1 year's follow up,they were divided into non-rejection group (group A) and rejection group (group B).36 healthy controls (group C) were also randomly recruited.Left ventricular ejection fraction (LVEF),pulmonary arterial systolic pressure,tricuspid annular plane systolic excursion (TAPSE),tricuspid annular plane systolic velocity (TAS') and global longitudinal strain (GLS) were calculated via Qlab 9.0 analysis software offline.Results Compared with group C,LVEF was reduced in group B (P ＜0.01).Differences of GLS,TAPSE,and TA-S' among groups were all statistically significant (P ＜0.01),group B＜group A＜ group C.LVEF,TAPSE and TA-S' were correlated with GLS (r =-0.64,r =-0.69,r =-0.71 ; all P ＜ 0.01).Conclusions Left and right ventricles were a functional unity,the systolic function of which was impaired in patients with heart transplant.

The paper constructs the evaluation index system for novelty retrieval organizations according to the demand of quality evaluation in these organizations .This index system and its weight coefficients were determined using Delphi and Analytical Hierarchy Process ( AHP) methods.The whole evaluation index system based on novelty retrieval regulations , comprises of four first-level indicators and fourteen second-level indicators .The index system facilitates the construction of novelty retrieval organizations and the improvement of novelty retrieval quality .It also provides reference for setting evalua-tion standards of military medical novelty retrieval organizations .

Objective To understand the current status of hypertension in Jingzhuang Town,Yanqing District,Beijing. Method With randomized cluster sampling,3 653 residents aged over 30 yrs old were investigated by a standard questionnaire on hypertension epidemiology.Result The prevalence rate of hypertension in Jingzhuang Town was 33.3%,and increased with the age(P0.05).The main patients were those with degree 1 hypertension(62.8%).There were 37.8%of people with normal blood pressure who had high-normal values,the highest(44.3%)in 60～69 yr group.In population with hypertension,the rate of people taking medication was 24.5%,disease control rate was 8.6%,while in patients aged 30～49 yrs,these rates were 7.1%and 2.9% respectively.Conclusion The prevalence of hypertension in Beijing's suburb was serious with a younger trend.There was a large part of people with high-normal blood oressure.