Objective:To explore the effects of acupoint massage combined with transcranial direct current stimulation on cognitive function and quality of life in patients with spontaneous cerebral hemorrhage, to provide direction for early nursing intervention of patients with spontaneous cerebral hemorrhage.Methods:A total of 214 patients with spontaneous cerebral hemorrhage in First Affiliated Hospital of Zhengzhou University from February 2018 to May 2020 were selected as the research objects. They were divided into 2 groups by random number table method: control group (107 cases) and observation group (107 cases). The control group was given transcranial direct current stimulation intervention, and the observation group was given acupoint massage intervention on the basis of the control group. The intervention lasted for 3 weeks. The intervention effects, cognitive function and quality of life of the two groups were evaluated by National Institutes of Health Stroke Scale, Montreal Cognitive Assessment, Stroke Specific Quality of Life Scale and compared.Results:The total effective rate was 91.59%(98/107) in the observation group, 80.37%(86/107) in the control group, and the difference was statistically significant ( χ2 value was 5.583, P<0.05). There was no significant difference in the cognitive function and quality of life before intervention between the two groups ( P>0.05). The visual space and execution, language, abstraction, delayed recall, orientation and total scores after intervention were (2.54 ± 0.51), (2.35 ± 0.37), (1.96 ± 0.31), (2.78 ± 0.38), (2.88 ± 0.41), (17.48 ± 2.01) points in the observation group, and (2.30 ± 0.62), (2.13 ± 0.36), (1.54 ± 0.38), (2.25 ± 0.43), (2.52 ± 0.38), (15.66 ± 1.89) points in the control group, and the differences were statistically significant ( t values were 3.092-9.554, P<0.05). The energy, family role, mood, personality, language, activities, self-care ability, social role, thinking, upper limb function, vision, work/labor and total scores after intervention were (12.85 ± 2.16), (12.94 ± 3.17), (23.46 ± 3.87), (12.53 ± 3.02), (23.76 ± 4.03), (27.52 ± 4.48), (24.92 ± 3.27), (20.94 ± 4.07), (13.15 ± 2.00), (24.54 ± 4.30), (12.20 ± 2.58), (13.54 ± 2.38), (222.35 ± 24.38) points in the observation group, and (10.04 ± 2.02), (10.52 ± 3.03), (21.25 ± 4.02), (11.15 ± 3.00), (21.46 ± 3.31), (24.37 ± 4.32), (22.38 ± 3.05), (18.60 ± 4.02), (12.47 ± 2.05), (22.08 ± 4.21), (11.42 ± 2.63), (11.05 ± 2.51), (197.49 ± 20.06) points in the control group, and the differences were statistically significant ( t values were 2.190-9.829, P<0.05). Conclusions:Acupoint massage combined with transcranial direct current stimulation can improve the cognitive function and quality of life of patients with spontaneous cerebral hemorrhage, and the effect is definite.

Objective To assess the anticonvulsant effect of midazolam, thiopental and propofol on the convulsion induced by ropivacaine in rats and the underlying mechanism.Methods Fifty male SD rats, 4-6 months of age, weighing 250-300 g were randomly divided into five groups of 10 animals each : (1) control group (C); (2) ropivacaine group; (R) (3) midazolam-ropivacaine group (MR); (4) thiopental-ropivacaine group (SR) and (5) propofol-ropivacaine group (PR) . In control group normal saline 2 ml was infused i.v. . In ropivacaine group (R) 0.75% ropivacaine was infused i.v. at 0.5 ml?min-1 until convulsion occurred. In midazolam - ropivacaine group (MR) midazolam 0.23 mg?kg-1 was injected i.v. 3 min before 0.75% ropivacaine infusion. In SR and PR groups thiopental 2 mg?kg-1 or propofol 1 mg?kg-1 was injected i.v. 1 min before 0.75% ropivacaine infusion. As soon as convulsion occurred ropivacaine infusion was stopped. The animals were decapitated and their brains were removed and kept in liquid nitrogen for determination of the contents of excitatory ammo-acids (aspartic acid and glutamic acid) and the inhibitory amino-acids [glycine and ?-amino butyric acid (GABA)] by high-performance liquid chromatography . The amount of ropivacaine infused in each group was recorded and calculated.Results The dose of ropivacaine needed to produce convulsion was 4.6?0.5 mg?kg-1 in group R and was significantly increased in the 3 groups pretreated with general anesthetics and was 2.96 times the dose in group R in MR group, 2.84 times in SR group and 1.54 times in PR group. The 4 amino-acid concentrations in the brain were significantly higher in group R than in control group. Compared to group R the Asp, Gly and GABA concentrations were significantly lower in group MR and PR but in group SR only Asp concentration was significantly lower. There was no significant difference in glutamic acid concentration among the4 roopivacaine groups (group 2-5).Conclusion Midazolam, thiopental and propofol can all increase the dose of ropivacaine needed to induce convulsion and midazolam and thiopental are more effective than propofol.

Objective To explore the current status of sexual education and real needs of college freshmen in Kunming.Methods By applying random sampling,350 students from three universities in Kunming were investigated by seff-administered questionnaire,and 310 valid questionnaires were recovered.Fesults 23.9％ students confirmed that the schools do not provide courses related to sexual health,and 22.9％ of the surveyed students claimed that they even did not heard any sexual education courses in their schools.The main sources of students to acquire information in terms of sexuality and sexual health are network television,video magazines,friends and classmates.Both male and female students are more acceptable to carry out sex education lectures.There were significant differences between male and female students in the form of obtaining sexual education,the content of sexual education and the demand of sexual education in three aspects (P＜0.05).Girls tend to carry out sexual education in the form of major courses,but boys tend to the form of playing video or distributing books and periodicals (P＜0.05).Girls tend to obtain the courses on sexually disease,AIDS,heterosexual intercourse,or love psychology,but boys tend to contraceptive knowledge and skilIs,or Sexual value orientation (P＜0.05).Girls are more likely to acquire the knowledge of masturbation,sexual health,or sexual health,but boys prefer to the knowledge of sexual psychology,parasexuality,or homosexuality (P＜0.05).Conclusion As a main body of educational institute for young people,all universities should provide training courses related to sexuality and sexual health at the early stage of higher education in order to equip college students with essential knowledge and skills to have safer and positive impact on their college studies.

Objective To investigate the influence of different hemostatic way on ovarian function in laparoscopic excision of ovarian cysts. Methods 87 cases with unilateral ovarian cysts by laparoscopic excision were chosen from Jun. 2012 to Jun. 2013 in our hospital, and they were divided into the electrocoagulation group (44 cases) and the suture group (43 cases) according to hemostatic way. The electro-coagulation group were given bipolar coagulation while the suture group were given endoscopic hemostasis. The ovarian hormone levels and blood parameters before and after surgery were compared between the two groups. Results There was no statistically significant differenc in parameters of E2,FSH,LH,P before surgery between the two groups (P>0. 05), and there was no statistically significant differenc before and after surgery in the same group (P>0. 05). Compared with the suture group, there were statistically significant difference in terms of pa-rameters of E2,FSH in the electrocoagulation group(P<0. 05). There was no statistically significant difference in ovary sectional area and PSV between the two groups before surgery (P>0. 05). It was of no statistically significant difference in ovary sectional area between the two groups after surgery (P>0. 05), but it was of statistically significance in PSV (P<0. 05). Conclusion Compared with electrocoagulation, the laparoscopic excision of ovarian cysts with endoscopic hemostasis had less damage to ovarian, and it could protect the ovarian function.

Objective To analyze the clinical characteristics and nursing measures of infection in hospital ICU,and to provide the refer-ence for clinical nursing. Methods From January 2010 to June 2013,116 patients with nosocomial infection in surgical ICU were collected as observation group,and 72 patients with nosocomial infection in the surgery were as the control group. The risk factors of nosocomial infec-tion were retrospectively analyzed,and the problems related to nursing were discussed. Results The nosocomial infection rate was 8. 44% in ICU,which was significantly higher than the surgical infection rate(4. 43%) in general ward (P<0. 05). Whether patients in ICU or surgery ward,respiratory and urinary tract infections were the most common site of nosocomial infection. The mortality rate in ICU was 9. 48%,which was higher than that of nosocomial infection in surgical ward (4. 17%),P<0. 05. The nosocomial infections were obviously related to the age of patients,duration of hospitalization,the invasive operation,antibiotic and immunosuppressive agents and disturbance of consciousness in ICU and general wards (P<0. 01). Conclusion The ICU nosocomial infection rate was significantly higher than that in general ward,the main infection sites are respiratory tract and urinary tract,with a variety of factors,the key is to establish the mechanism of prevention and ef-fective nursing strategy.

0.05). Positive stains were located in cytoplasm of cancer and stromal cells, especially in invasive margin of cancer. Slight positive stains were observed in non-cancer tissues. But in the control samples, there wasn't specific stain. In ELISA, the uPA antigen levels in cancer and non- cancer tissues were (4.23?0.57)ng/mg protein and (1.26?0.14)ng/mg protein; the uPAR antigen levels in caner and non-cancer tissues were(4.25?0.21)ng/mg protein and (3.15?0.23)ng/mg protein respectively. Significant difference existed in both uPA and uPAR antigen levels in these two kinds of tissue ( t=18.963,P=0.000;t=13 693,P=0 000 ). Furthermore, these antigen levels in progressive and aggressive tumors significantly higher than those in non-progressive and non-aggressive tumors ( P

OBJECTIVE To investigate the prevalence of meticillin-resistant Staphylococcus haemolyticus(MRSH)and resistance in S.haemolyticus isolates. METHODS MRSH was detected by cefoxitin disc test and susceptibilities were tested by agar dilution method. RESULTS There were 86.4% of MRSH isolates.MRSH was highly resistant to penicillin,cefazolin,cefuroxime,ceftriaxone,tetracycline,ciprofloxacin,and clindamycin.The resistance rate to amikacin,rifampicine and chloramphenicol was 16.9%,11.2% and 28.1%,respectively.All isolates were susceptible to vancomycin and teicoplanin.Except for tetracycline,amikacin,rifampicin,and chloramphenicol,the resistant rate to other antimicrobial agents was significantly higher in MRSH than in MSSH. CONCLUSIONS MRSH is mostly occupied in S.haemolyticus isolates and resistant to most of antimicrobial agents.More attention should be paid to survey and detect these strains.

BACKGROUND:Bone marrow mesenchymal stem cellcontent is less under normal conditions, and easily confounded with other cells. Therefore, to establish a simple feasible in vitro cultured amplification method and to obtain a large number of stable bone marrow mesenchymal stem cells are of important theoretical significance and application value. OBJECTIVE:To establish a simple feasible in vitro culture and amplification method and to obtain numerous stable bone marrow mesenchymal stem cells. METHODS:Bone marrow mesenchymal stem cells were isolated from Sprague-Dawley rats and cultured in vitro using density gradient centrifugation and adherence separation screening. cellmorphological changes were observed under an inverted light microscope. Submicroscopic structure of cells was observed under a transmission electron microscope. Living cellnumber was counted using Trypan blue exclusion method. cellgrowth curve was drawn. cellcycle was analyzed using flow cytometry. The expression of c-kit and CD45 was measured using immunocytochemistry. CD45 expression was analyzed using flow cytometry. RESULTS AND CONCLUSION:(1) celladhesion and pseudopodia could be seen under an inverted light microscope at 24 hours after inoculation;cellcolony formed at 4 days;90%cellconfluence was found at 14 days. After passage, the cells were tended to homogenous and became fibroblast-like cells, which showed whirl-like growing or flamboyancy growing. (2) The size of passage 3 bone marrow mesenchymal stem cells was smal and nucleolus was large, with clear nucleoli under a transmission electron microscope, with the presence of sparse chromatin and low electron density. There was microvil us on the surface of cells, abundant ribosomes could be seen in the cytoplasm with few other cellular organs such as endoplasmic reticulum, mitochondria and Golgi complex. These showed that ultrastructural structure had undifferentiated features. (3) The number of passage 3 bone marrow mesenchymal stem cells was reduced at 1 day after inoculation. The cells began to grow at 2 days, and entered the period of exponential growth at 3 days, entered the flat period at 7 days and the number of cells began to decrease at 9 days. Growth curve exhibited“S”shape. (4) The percentage of S phase cells was 21.1%as detected by flow cytometry after passage 3 bone marrow mesenchymal stem cells were stained by DNA. (5) Immunocytochemistry and flow cytometry had proved that the c-kit positive rate of cells was 53.3%and CD45 positive rate was 1.68%. (6) After osteogenic induction of mesenchymal stem cells for 16 days, cells became oval, and short processes connected each other. The cytoplasm was dark, suggesting that it may be rich in rough endoplasmic reticulum and Golgi complex. They can secrete osteoid. Results verified that obtained bone marrow mesenchymal stem cells stably grew, and actively proliferated.

Objective To construct a finite element analytical model of multiloop edgewise arch wire(MEAW),and study its mechanical characteristics.Methods Three-dimensional(3D) model of dentition-bracket-arch wire was set up in an initial graphics exchange specification(IGES) format complex by Pro/E software.The multiloop edgewise arch wire was spliced on the lower dentition.After dividing the arch wire and brackets with quarter-tetrahedron element method,the model was analyzed by ANSYS(an engineering simulation software).Results The finite element analytical model of multiloop edgewise arch wire was successfully built up.Based on the model,when multiloop edgewise arch wire was subjected to a heavy strength in certain parts(such as tooth 3,4),the anti-force strength on the other brackets(such as tooth 1,2,5,6,7) was not so heavy.The subjected strength was light and soft,especially on the first molar.Conclusion The results suggest that the finite element analytical model of multiloop edgewise arch wire built up in the experiment may be used to study the integrated mechanical characteristics,reveal the orthodontic specialty,and discuss the orthodontic mechanisms of multiloop edgewise arch wire techniques.

Recently,a large family of G-protein coupled receptors (GPCRs) has been identified as mas-related genes (Mrgs),Which are specific expressed in small diameter sensory neurons in the trigeminal and dorsal root ganglia,suggesting a role in pain transmission.Mrgs receptors have been shown to modulate some physiological and pathological activities,such as pain and immunity.It is important to discover ligands of Mrgs for understanding and elucidating their potential physiological and pathophysiological roles.The studies on pharmacological spectrum of NPS make it an interesting target for pharmaceutical development.