Objective To synthesize of water-solubility derivant of 6,7,4′- trihydroxyisoflavone(T2 ),to characterize its structure and to evaluate its anti-tumor activity.Methods The sulfonic group(-SO3 H)was grafted to 3'position in loop B of T2 through sulfonation reaction,then strong aqua was added to above production,and obtained a water-solubility elevated compound named T2-SO3 (NH4 )2 through ammoniation.The construction of T2-SO3 (NH4 )2 was characterized by 1 H-NMR,13 C NMR,MS and element analysis.Its activity for killing human breast cancer cells (Hela)was analyzed by CCK-8 assay and flow cytometry.Re-sults The two kinds of water-solubility derivant of T2-SO3 H·2H2 O and T2-SO3 (NH4 )2 were obtained through above methods, and their yield rates were 96% and 75% respectively.The biological experiments showed that the anti-tumor activity of T2-SO3 (NH4 )2 was significantly enhanced.Conclusion Compared to T2,T2-SO3 (NH4 )2 exhibit higher biocompatibility and anti-tumor activity with vast biological application prospect.

Sleep deprivation (SD) has been taken as an independent predictor for cardiovascular risks, which was closely related to the increased morbidity and mortality in coronary heart disease (CHD). In this article, after reviewing the impact of modern medical method sleep deprivation on CHD and studies on principle method recipe medicines for preventing and treating CHD, the authors observed the autonomic nerve dysfunction, hormonal metabolism dysfunction, endothelial dysfunction and inflammatory responses after sleep deprivation, which can cause or aggravate CHD. On the basis of the traditional Chinese medicine theories of "heart dominating the blood and vessels and the mind", the authors considered that traditional Chinese medicines can tonify heart and soothe the nerves, reducing all of the risk factors through multi-target and multi-pathway, and improve sleep and decrease the risk factors caused by sleep deprivation, which provides a new idea for the prevention and treatment of CHD.
Animals ; Coronary Disease ; drug therapy ; etiology ; prevention & control ; Drugs, Chinese Herbal ; administration & dosage ; Humans ; Sleep Deprivation ; complications

Child, Preschool ; Diagnosis, Differential ; Humans ; Male ; Myositis Ossificans ; diagnosis ; diagnostic imaging ; pathology ; physiopathology ; Prognosis ; Tomography, X-Ray Computed

Asaprimarilyused plasticizerin polyvinyl chloride,d(i2-ethylhexy)lphthalate(DEHP)is extensively used in consumer products,flooring and wall coverings,food contact applications,and medical devices.Because DEHP has been detected in waters,soils and air,and its metabolites have also been detected in human urinary,sufficient evidences show that DEHP is ubiquitous in the environment worldwide.DEHP is already in the blacklist of priority pollutants in water in China.Many researches indicate that DEHP is one kind of endocrine disrupting chemicals in animals,with carcinogenicity,teratogenicity and mutagenicity.Based on the researches and review on DEHP,this paper principally discussed the environmental level,metabolism and biological markers of DEHP,as well as the research progress on human health effects.

Event-Related Potentials, P300 ; physiology ; Humans ; Individuality ; Male ; Memory, Short-Term ; physiology ; Neuropsychological Tests ; standards ; Young Adult

Antineoplastic Agents ; administration & dosage ; therapeutic use ; Child ; Female ; Humans ; Injections, Intralesional ; Interferon-alpha ; administration & dosage ; therapeutic use ; Male ; Neoplasms ; drug therapy ; Recombinant Proteins ; Treatment Outcome

Objective To analyse the clinical and pathological characteristics,diagnosis,treatment and prognosis of vulvar Bowen's disease.Methods Clinical data including pathological characteristics,diagnosis,treatment methods and follow-up of 18 cases with vulvar Bowen's disease admitted to Cancer Hospital,Chinese Academy of Medical Sciences during January 1991 to June 2011 were retrospectively analyzed.Results The median age of the 18 patients was 37 years (range:23 to 64 years).Sixteen patients had symptoms of vulvar itching and two patients had no symptom.Five cases were single neoplasm focus and the other 13 cases were multiple focuses.The diagnosis of vulvar Bowen's disease was according to the pathological diagnosis.Its diagnostic characteristic was giant round or ovoid cells with mono nucleolus in the whole layer of epidermis.All the patients received operation,eleven with simple vulvectomy and other seven cases with lumpectomy.The median follow-up time was 123 months (range:5 to 197 months).Relapse was found in two cases.One patient relapsed five months postoperation and received vulvectomy.Another patient relapsed fifteen moths post-operation and received lumpectomy again.And they were follow-up for 192months and 55 months respectively after second operation without relapse.Conclusions The diagnostic characteristic of vulvar Bowen's disease is giant round or ovoid cell with mono nucleolus in the whole layer of epidermis,itsdiagnosis is according to the pathological diagnosis.Operation could get very good curative effect for patients with primary vulvar Bowen's disease and even for the recurrent patients.The prognosis of vulvar Bowen's disease is good.

Carcinoma, Squamous Cell ; metabolism ; pathology ; Cell Line, Tumor ; Humans ; Laryngeal Neoplasms ; metabolism ; pathology ; Matrix Metalloproteinase 9 ; biosynthesis ; genetics ; Oncogene Proteins, Viral ; genetics ; Papillomavirus E7 Proteins ; Plasmids ; RNA, Messenger ; metabolism ; Repressor Proteins ; genetics ; Transfection ; Vascular Endothelial Growth Factor A ; biosynthesis ; genetics

Objective To analysis the constituents of urinary stones in southeast Chongqing district,and provide the percep-tion and treatment of urinary stones.Methods Retrospective analysis of 1 628 random urinary stones samples in southeast Chongqing district from 2008 to 2014.The chemical constituents of stones were analysis with the clinical data.Results Urinary stones were more often found in those 30 to 70 years old,especially in males.The upper system stones were frequently founded.The percentage of mixed stones and pure stones were similar,The constituents of stones were similar to male and female,the most com-mon component of the urinary stones are calcium oxalate,which were found in 82.9% of the stones,infection stones were found 1 9.2%,but only found in renal and ureteral stones,and also increase gradually.The most common component of urinary stones in Qianjiang and Pengshui district were calcium oxalate,the infection stones were frequently found in Youyang and Xiushan district. Conclusion Chemical composition analysis of urinary stones is simple,quick and accurate,it is very important in providing infor-mation to the etiology,treatment and prevention of urinary stones.

BACKGROUND:Structural and functional changes of endothelial cels are the common pathological basis of cardiovascular disease. Severe structural and functional damage of endothelial cels are found in patients with hypertension or coronary heart diseases.
OBJECTIVE:To explore a new treatment method for hypertension from the perspective of vascular endothelial progenitor cels.
METHODS: PubMed and Wanfang databases were retrieved using the keywords “hypertension, EPCs” and approximately relevant 200 English and 100 Chinese literatures were obtained. Forty-nine eligible literatures were screened finaly.
RESULTS AND CONCLUSION:Endothelial progenitor cels have strong differentiation and proliferation capacities. This review may provide a new insight into potential sources of cels for diagnosis and treatment of hypertension.