1.The roles and administration methods of insulin in the early treatment of acute brain injury
Jiang TANG ; Limin ZHOU ; Han JIANG ; Rong LI ; Jianqiang HAN
Journal of Chinese Physician 2012;(11):1472-1474
Objective To explore the roles of insulin in the early treatment of acute brain injury and its administration methods.Methods 253 patients were randomly divided into the intensive insulin therapy group and the conventional therapy group.Infection rates,the short-term effect (APACHE Ⅱ assessment),and long-term efficacy (GOS prognosis) was compared between two groups.Results The results of the strengthen treatment group in the rate of infection (25.95% vs 39.34%,x2 =5.17,P <0.05),the short-term effect (11.33 ± 7.66 vs 16.49 ± 14.97,u =3.42,P < 0.05) and the long-term efficacy (40.46%,55.73%,3.82% vs 25.41%,68.85%,5.74%,x2 =7.62,P <0.05) were significantly better than the conventional therapy group with the statistically significant differences (P < 0.05).Conclusions The hypoglycemic effect,neuroprotective effect,regulatory role,and nutrition role of insulin occurred in the early treatment of acute brain injury.After acute brain injury,patients with hyperglycemia should be treated early with an enough volume,continuous,and uniform insulin.
2.Effects of midazolam on hERG K+ channel.
Sheng-na HAN ; Pei WANG ; Wei ZHANG ; Li-rong ZHANG
Chinese Journal of Applied Physiology 2015;31(2):143-147
OBJECTIVETo investigate the effect of midazolam on human ether-a-go-go (hERG) K+ channels exogenously expressed in human embryonic kidney cells (HEK-293) and the underlying molecular mechanisms.
METHODSWhole-cell patch clamp technique was used to record WT, Y652A and F656C hERG K+ current expressed in HEK-293 cells.
RESULTSMidazolam inhibited hERG K+ current in a concentration-dependent manner, the half-maximum block concentrations (IC50) values were (1.31 ± 0.32) µmol/L. The half-activation voltage (V1/2) were (2.32 ± 0.38) mV for the control and (-1.96 ± 0.83) mV for 1.0 µmol/L midazolam. The half-inactivation voltage (V1/2) was slightly shifted towards negative voltages from (-49.25 ± 0.69) mV in control to (-57.53 ± 0.53) mV after 1.0 µmol/L midazolam (P < 0.05). Mutations in drug-binding sites (Y652A or F656C) of the hERG channel significantly attenuated the hERG current blockade by midazolam.
CONCLUSIONMidazolam can block hERG K+ channel and cause the speed of inactivation faster. Mutations in the drug-binding sites (Y652 or F656) of the hERG channel were found to attenuate hERG current blockage by midazolam.
Dose-Response Relationship, Drug ; Ether-A-Go-Go Potassium Channels ; drug effects ; HEK293 Cells ; Humans ; Midazolam ; pharmacology ; Mutation ; Patch-Clamp Techniques ; Potassium Channel Blockers ; pharmacology
3.Application and future prospect of 18F-FLT PET-CT in guiding delineation of biological target volume.
Da-li HAN ; Wan-rong JIANG ; Jin-ming YU
Chinese Journal of Oncology 2009;31(1):1-4
Dideoxynucleosides
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False Positive Reactions
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Fluorine Radioisotopes
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Fluorodeoxyglucose F18
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Humans
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Inflammation
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diagnosis
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Neoplasm Staging
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Neoplasms
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diagnosis
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metabolism
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pathology
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radiotherapy
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Positron-Emission Tomography
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methods
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Radiotherapy, Intensity-Modulated
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Sensitivity and Specificity
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Tomography, X-Ray Computed
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Treatment Outcome
4.The strategies of bone marrow mesenchymal stem cells proliferation in vitro
Ruini LI ; Weiwei RONG ; Shizhu JIN ; Mingzi HAN
Journal of Medical Postgraduates 2015;(5):549-552
The approach of stem cell transplantation to treat different types of diseases has been becoming a hot issue in the field of regenerative medicine.Potentially, bone marrow mesenchymal stem cells (BMMSCs) possess strong proliferation and multi-di-rection differentiation ability .Under special certain kind of induction circumstances , BMMSCs can trans-differentiate into myocardial cells, osteoblasts, fat cells, neurons, endothelial cells.It is difficult that how to obtain enough BMMSCs for basic and clinical studies using BMMSCs treating diseases .Currently , there are many methods of in vitro proliferation of BMMSCs with advantages and disadvan -tages.In this paper, an overview of BMMSCs proliferation strategy in vitro is provided .
5.Validation method for microbial limits of A,wei Capsule
Rong HAN ; Changlin GAO ; Xiaoyan LI ; Jie WUE
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(12):-
Objective:To eliminate the bacteriostatic action ofA,wei Capsule in microbial limit examination so as to establishing the method ofmicrobial limit for A,wei Capsule.Methods:The plating and culture medium diluted methods were used to determine the antimicrobial effect ofA,wei Capsule by recovery rates with 5 control trains.Results:According to the results, the recovery rates were higher than 70% by culture medium diluted method, which indicated no antimicrobial effect with them.Conclusion:According to the results, the culture medium diluted method can eliminate the bacteriostatic action ofA,wei Capsule preferably with accurate result and reaching the objective.
6.Adjusting effect of human chorionic gonadotrophin in the vascular endothelial growth factor in rabbit phallus with hypospadias
Xiaofeng YANG ; Debiao LI ; Xuebing HAN ; Dongwen RONG ; Jing LIU
Chinese Journal of Urology 2008;29(9):643-645
Objective To study the function of human chorionie gonadotrophin(HCG) in adjus-ting the vascular endothelial growth factor(VEGF) in rabbit phallus with hypospadias. Methods Rabbit hypospadias mode was made by Finasteride. After the spontaneous delivery, 35 rabbits were divided into 5 groups with 7 in each group. Four groups accepted HCG intramuscular injection for 7 consecutive days with dosages of 100,200,400 and 600 U, respectively. The control group accepted the same dosage of saline injection. Another 7 normal rabbits were used as normal controls without in-tervention. After 3 weeks, the rabbit phallus tissue was collected and the VEGF levels were detected by ELISA. Results Rabbits with hypospadias accepted HCG 100, 200, 400, 600 U injection had the phallus tissue VEGF level of 5.00±2.37,5.63±1.73, 10.35±2.34 and 16.91±2.34 pg/ml, respectively. While the rabbits accepted saline injection had VEGF levels of 3.99±1.19 pg/ml. The normal rabbit phallus tissue contained VEGF level of 14.82±3.32 pg/ml. There were significant differences between normal group and the rabbits accepted HCG 100, 200, 400 U injection (P< 0.05), but there was no difference with the rabbits accepted HCG 600 U injection (P>0.05). The VEGF level in rabbits accepted 400 U HCG injection had significant difference with rabbits accepted 100, 200, 600 U HCG (P<0.05). The VEGF levels in rabbits accepted 100, 200 U HCG injection had no difference with rabbits accepted saline injection(P>0.05). Conclusions The VEGF in rabbit phallus with hypospadias is decreased. HCG of certain dosage could increase VEGF level in rabbit phallus with hypospadias.
7.Passive Smoking in Beijing Public Places
Yu-Qing LI ; Xiu-Rong LIU ; Mei HAN ;
Chinese Journal of Prevention and Control of Chronic Diseases 2006;0(05):-
0.05).92.7%of the objects knew that passive smoking was harmful.71.9%knew that passive smoking made people suffering from cardiopathy more possibly.74.9%knew that the wife whose husband smoked were easier to catch lung cancer.And 84.4%knew that the child whose parent s smoked more possibly took asthma or respiration disease. The correct rates of the four knowledge points were different among different gender and the degree of education,which was higher in female than in male,and higher in high education degree than in the other(P
8.Effects of Inula Britannica on myocardial caspase-3 and cytochrome C expression following overtraining-induced acute myocardial injury in rats
Shuang HAN ; Yunxin WANG ; Wei CHEN ; Shenghong LI ; Junfang RONG
Chinese Journal of Anesthesiology 2012;32(1):107-109
Objective To investigate the effects of Inula Britannica on myocardial caspase-3 and cytochrome c ( cyt c) following overtraining-induced acute myocardial injury in rats.Methods Forty-eight male Wistar rats weighing 200-220 g were randomly divided into 3 groups:group control (group C,n =8) ; group exhausting swim (group E,n =24) and group Inula Britannica (group IB,n =16).The animal model of overtraining-indnced acute myocardial injury was developed by exhausting swim.The animals were forced to swim until they were exhausted.The animals sank to the bottom and no righting reflex or escape response was elicited when they were taken out of water in groups E and IB.In group IB oral Inula Britannica 25 ml/kg was given 24 h and immediately before overtraining.Blood samples were taken from inferior vena cava immediately and at 6,24 h after overtraining in group E and at 6,24 h after overtraining in group IB for determination of serum cardiac troponin I (cTnI) concentration (by ELISA).The animals were sacrificed after blood sampling and myocardial specimens were obtained for microscopic examination and determination of caspase-3 and cyt c expression (by immuno-histochemistry).Results Overtraining significantly increased serum cTnI concentration and up-regulated myocardial caspase-3 and cyt c expression in group E as compared with group C.Oral Inula Britannica significantly attenuated overtraining-induced increase in serum cTnI concentration and myocardial caspase-3 and cyt c expression in group IB as compared with group E.Conclusion Inula Britannica can reduce overtraining-induced acute myocardial injury by down-regulating caspase-3 and cyt c expression.
9.Association of HLA-Cw and -DRB1 alleles with psoriasis vulgaris in Mongolian population
Wenchao ZHAO ; Li SUN ; Jianwen HAN ; Rong HAI ; Rina WU
Chinese Journal of Dermatology 2012;45(6):408-410
ObjectiveTo investigate the association of HLA-Cw and -DRB1 alleles with psoriasis vulgaris,and to provide a clue to the study into the etiology of psoriasis.MethodsVenous blood samples were obtained from 81 patients with psoriasis vulgaris collected during 2006-2011 at the Department of Dermatology,First Affiliated Hospital of Inner Mongolia Medical College,as well as 100 age- and gender-matched healthy controls.Both the patients and controls are unrelated Mongolia in Inner Mongolia.PCR with sequence-specific primers(PCR-SSP) technique was used to genotype the HLA-Cw and DRB1 loci.ResultsThe patients with psoriasis vulgaris showed a significantly higher frequency of HLA-Cw*06(0.438 vs.0.175,Pc < 0.01) and DRB1*07(0.241 vs.0.110,Pc < 0.012),but a lower frequency of HLA-Cw*04(0.031 vs.0.150,Pc < 0.01 ) and DRB1*04 (0.093 vs.0.235,Pc < 0.01 ) than the healthy controls did.Increased frequencies of HLA-Cw*06 and DRB1*07 alleles were observed in patients with an onset before 40 years of age and those without a family history,together with a decreased frequency of HLA-Cw*04 and DRB1*04 alleles,compared with the healthy controls(Pc < 0.05).The frequency of HLA-Cw*06 allele was significantly higher in patients with a positive family history and patients with an onset of no younger than 40 years of age than in the healthy controls (both Pc < 0.05).ConclusionsHLA-Cw*06 and -DRB1*07 alleles may be susceptibility determinants to psoriasis vulgaris,while HLA-Cw*04 and -DRB1*04 alleles may be protective factors against psoriasis vulgaris,in Mongolia from Inner Mongolia.HLA-DRB1*07 allele may be a susceptibility gene for psoriasis,while HLA-Cw*04 and -DRB1*04 alleles may be protective factors against psoriasis,in patients with an onset before 40 years of age.